Comamonas Research Articles

The analysis of pathogens and drug sensitivity spectrum in acute appendicitis in children

Objective To study the major pathogens of the abdominal infection in children with acute appendicitis recently, and analyze the resistance of pathogens, combined with clinical efficacy, to learn about the spectrum of pathogens and sensitive antibacterial drugs, so as to guide rational use of antibacterial drugs. Methods Four hundred and ninety-three cases of pus specimens of acute appendicitis in children were tested by phoenix100 bacterial identification system for bacterial culture and sensitivity test, and the main types of pathogens and drug sensitivity were analyzed, and the treatment was followed up and the main pathogens and drug susceptibility spectrum were summarized. Results There were simple, suppurative and gangrenous appendicitis in this group, in which one single kind of bacte-rial infection in 1 case, 63 cases and 143 cases respectively; while two kinds in 0 case, 26 cases, and 62 cases respectively; and there was 1 case of gangrenous appendicitis with three kinds, though the comparison between pathological type appendicitis and bacterial infection type indicated that there was no significant difference(χ2=0.898, P>0.05). Four hundred and ninety-three cases of bacterial samples were detected with pathogens 296 cases, and the positive rate was 60.04%, 386 strains of bacteria were isolated, 341 strains(88.34%) of gram negative bacteria, 45 strains (11.66%) of gram positive bacteria; extended-spectrum β-lactamase producing strains were detected in total 126 strains(32.64%). The top three gram negative bacteria and gram positive bacteria were Escherichia coli(65.28%), Pseudomonas aeruginosa(12.18%), Comamonas testosteroni(5.44%)and Streptococcus anginosus(5.18%), Streptococcus constellatus(3.11%), Enterococcus raffinosus(0.78%), respectively.The sensitive rates of Escherichia coli, Pseudomonas aeruginosa, Comamonas testosteroni to Piperacillin/Tazobactam and Imipenem(tienam), Meropenem and Amikacin≥88.1%; the resistance rates of them to ampicillin, Tetracycline, Cotrimoxazole≥61.9%.The treatment program was Ceftizoxime sodium + Piperacillin/Tazobactam sodium + Metronidazole or Ornidazole. Conclusions The main pathogens are gram-negative bacteria in children appendicitis, and the most common bacteria is Escherichia coli, and it has significant resistance to the broad-spectrum Penicillins, Cephalosporins; In order to reduce the emergence of drug-resistance strains, germiculture and susceptibility test should be enhanced, use antibacterial drugs appropriately. Key words: Appendicitis; Child; Pathogens; Bacterial culture; Susceptibility test; Antibacterial drugs

Comparison of p-toluenesulphonic acid degradation by two Comamonas strains

Reference LBE-ARTICLE-1997-005doi:10.1023/A:1018319421605View record in Web of Science Record created on 2005-03-02, modified on 2016-08-08

Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10.

A Comamonas acidovorans strain, designated NBA-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. When grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. In cell extracts with NADPH present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. Partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-nitrosobenzoate to 4-hydroxylamino-benzoate. The substrate specificity of the enzyme was narrow and NADPH was 15 times more effective as a cofactor than NADH. The results provide evidence for a novel pathway for aerobic degradation of 4-nitrobenzoate, since neither 4-hydroxybenzoate nor 4-aminobenzoate were involved in the degradative pathway.