Reinforced concrete (RC) columns play a crucial role in the overall performance of modern RC frame buildings under blast loading. The RC columns under blast loads might experience various irreversible consequences such as concrete cracks, crushing and spalling, changing the status of both modal properties and load-carrying capacity. In this paper, the blast-induced damage assessment of the RC columns involving the measurement of modal parameters was introduced and validated. Firstly, a modal-based method for blast-induced damage assessment of RC columns was proposed, in which the damage is characterized as the reduction of axial bearing capacity and further expressed as the explicit function of the vibration frequencies and the mass-normalized displacement mode shapes. Then, the finite-element analysis program LS-DYNA was employed to establish the numerical model of RC columns under axial compression loads and lateral blast loads. The residual load-bearing capacities and modal properties of the RC columns were illustrated. By using the numerical results collected in the present study, the validity of the proposed damage assessment method was discussed and proved numerically. Further, field explosion tests and experimental modal tests were conducted, and the results showed that the modal-based damage assessment method exhibited remarkable agreement with the blast damage determined by residual bearing capacities. Based on the numerical and experimental results, the proposed modal-based damage assessment method is applicable for the non-destructive evaluation of blast-induced damage of RC columns.