Inheritance Of Color Research Articles

High-Density Genetic Map Construction and QTL Detection for Cotyledon Color in Faba Bean Based on Double Digest Restriction-Site Associated DNA Sequencing (ddRAD-Seq)

Cotyledon color is one of the important indices for identifying faba bean variety purity and measuring processing quality. Therefore, an in-depth study of the genetic mechanism of cotyledon color is vital for promoting faba bean industry development. We used the yellow cotyledon variety Qingcan 16 and the green cotyledon variety Qingcan 17 as parent plants to construct hybrid combinations. F1-, F2-, BC1F1-, and BC2F1-generation single-plant cotyledon colors were counted to clarify cotyledon color inheritance. F2-generation individuals were genotyped using ddRAD-Seq to construct a genetic linkage map and identify QTLs for cotyledon color. Green cotyledons were controlled by one pair of recessive nuclear genes. Using the screened 1991 SNP markers, a high-density linkage map was constructed, with a coverage length of 1476.95 cM and an average map distance of 0.96 cM. The green cotyledon trait was located using WinQTL Cart, and a vfGC candidate interval explaining 34.30 to 49.40% of the phenotypic variation was identified at LG02 (101.952 cM to 115.493 cM) and at LOD = 16.0, corresponding to chr1L 1,077,051,302 bp to 1,636,400,339 bp (559.35 Mb). The above interval contained 2021 genes, 20 of which were involved in photosynthesis, but no SGR or genes with similar functions were identified. However, the published faba bean vfSGR was located within the vfGC candidate interval, confirming that our localization interval was reliable. The above findings provided further clues for the fine localization of genes regulating green cotyledons and the development of molecular linked markers in faba bean.

Inheritance of signs of parent species by hybrids of Russet, Yellow and Speckled ground squirrels (<i>Spermophilus</i>, Sciuridae, Rodentia)

The inheritance of size, color and bioacoustic characteristics of Russet (Spermophilus major), Yellow (S. fulvus) and Speckled (S. suslicus) ground squirrels in hybrids differing in their genetic status were studied. In a hybrid population of Russet and Yellow ground squirrels, 10 individuals of S. major, 10 individuals of S. fulvus and 40 hybrids of S. major × S. fulvus were analyzed; in a hybrid population of Russet and Speckled ground squirrels, 11 individuals of S. major, 11 individuals of S. suslicus and 24 hybrids of S. major × S. suslicus were analyzed. Hybrid individuals different in genetic status have been shown to demonstrate differentiation in the vector space of the variability range of the parental species. At the same time, both a shift in some categories of hybrids towards one of the hybridizing species and a significant deviation are noted. The results obtained indicate an increase in the level of variability in size, color and bioacoustic characters in hybrid populations, associated with the erosion of the divergent hiatus of specific features of the parental forms due to the formation of a differentiated hybrid population. As hybrid individuals with different combinations of parental traits accumulate in the population, the situation arises when a sufficient number of hybrids with an overall combination of parental traits (Hcomb) appear in the hybridogenic population. In the hybrid population of the Russet and Yellow ground squirrels, the proportion of such hybrids, according to our data, is 22.5% (n = 40), and in the hybrid population of the Russet and Speckled ground squirrels it is 12.5% (n = 24). This category of hybrid individuals can be considered as possible material for further microevolutionary events and the process of speciation.

V. Introduction and selection of a new species of Clarkia (C. purpurea) and development of a method for evaluating Clarkia pursh varieties for distinctiveness, uniformity and stability

During 2011–2023 in the south of Western Siberia, with the aim of introducing and selecting a new species of clarkia (C. purpurea), its biological characteristics and morphological characteristics were studied. Based on the data obtained and the use of other species and varieties of the genus Clarkia Pursh. For the first time, a methodology for conducting tests for distinctiveness, uniformity and stability (DUS) for this crop was developed. This technique is applicable to all varieties of the genus Clarkia Pursh, classified into sections: Godetia (C. purpurea (Curtis) A. Nelson & J. F. Macbr.), Phaeostoma (C. unguiculata Lindl.), Rhodanthos (C. amoena (Lehm.) A . Nelson & J. F. Macbr., Clarkia amoena ssp. lindleyi (Douglas) H. F. Lewis & M. Е. Lewis), fireweed family (Onagraceae Juss.). Many species of clarkia are characterized by long-lasting flowering and are promising for floral decoration of urban spaces, as well as for cutting. Most varieties are from very low – up to 25 cm tall, to very high – 96 cm or more; leaves are simple, lanceolate, narrow-lanceolate, wide-lanceolate, ovate, smooth, pubescent. The flowers are very decorative, varying in size from 2.0 to 8.0 cm tall, simple, semidouble, terry and strongly terry with or without fragrance, with a large variability of colors and types of floral pigmentation. Based on the study of the bioecological and morphological properties of the crop under study and in accordance with the rules approved by the Federal State Budgetary Institution “State Varietal Commission”, 36 characteristics were selected by which it is possible to test clarkey varieties. As a result of breeding work, a new variety of C. purpurea, Lilac Fairy, was created. When analyzing crossing, it was established that the lilac (violet) color of flowers is dominant in relation to pale pink (almost white), and when crossing F1 hybrids with light purple color, splitting 15:1 (15 violet from dark purple to pale purple was obtained and 1 is pale pink), which confirms the hypothesis about the polygenic nature of the inheritance of the main color of flowers in C. purpurea. The national methodology RTG/1157/1, created for the first time, for testing the DUS of Clarkia Pursh varieties will serve as a scientific basis for practical breeding.

PSIV-9 Pedigree tracing to determine the origin of the golden coat phenotype within the Golden American Saddlebred horse

Abstract With the incorporation of the Golden American Saddlebred Horse Association (GASHA) into the American Saddlebred Horse Association (ASHA), breeding specifically for coat color within the breed registry became less of an emphasis for horse breeders. Nevertheless, historical documentation of the horses making up the foundation of the GASHA through the application of pedigree tracing holds value in understanding the origin of the breed and its coat color. This can be of value to horse breeders targeting a specific coat color as the study of coat colors is a relatively new field of research within equine science. Thus, the objective of this study was to document the historical origins of the GASHA through pedigree tracing to determine the influence of the dilution genes that produce the golden coat phenotype. A random sample of 550 horses was obtained from the Official Directory of GASHA. Using the All-Breed Data Base, sampled horses were entered and examined for pedigrees suitable for analysis of color inheritance, and of these, 507 were found to have extended pedigrees with documented coat colors that were used for this study. Results determined that 43% of traced pedigrees carried the champagne gene, 36.1% carried the cream gene, and 20% had pedigrees that were non-informative as these pedigrees went to unknown ancestors or were found to be incorrect as to ancestry. Another 0.9% were determined to be obligate carriers for the cream gene; as the pedigrees presented conflict concerning color designation so that accurate conclusion of coat color phenotype associated with the cream dilution gene could not be verified. Of the 507, all traced back to 6 foundation mares and most of the breed traced back to 2 of these mares; with one mare, Maud, being a champagne mutation and the other, Allens Queen, a cream mutation (Table 1). In conclusion, through pedigree tracing the GASHA origins were documented giving an understanding as to the genetic contributions for today’s GASHA golden coat color. Further, these results will give breeders more input on how they might select for certain coat color phenotypes.

Inheritance of breeding traits in Karakul sheep under different selection approaches.

In the previous century, each intrabreed type of the Karakul sheep breed was characterized by significant numbers, representing a super population with rich genetic diversity. However, over time, the genetic diversity within the breed's gene pool has undergone significant depletion. At present, the Karakul breed is predominantly composed of only two small populations, distinguished by their fur colors: black and gray. Consequently, under such circumstances, genetic advancements in breeding endeavors are likely to be relatively limited, especially given the potential risk of these populations disappearing altogether in the future. Hence, the preservation and judicious utilization of the available genetic resources within the black and gray Karakul sheep populations hold paramount importance in breeding efforts. The primary objective of our research was to investigate the heritability of breeding traits among gray lambs through various selection options. The study was conducted at the "Kumkent" base farm in the Sozak district of the Kyzylorda region. Our findings revealed that the inheritance of gray and black fur colors across the different selection options occurred in a consistent ratio. In the first selection variant (a gray ram with even silver marking ♂ x a black jacket fur type ewe with intense pigmentation ♀), the proportion of gray offspring was 50.6%. Similarly, in the second selection variant (a black jacket fur type ram with intense pigmentation ♂ x a gray ewe with even silver marking ♀), the proportion of gray offspring was 49.6%. The percentage of black lambs obtained in both selection options was nearly equivalent, with 49.4% and 50.4% in the first and second variants, respectively.

Studies on inheritance of achene colour and markings among confectionary germplasm of sunflower

To study the genetic control of achene colour in sunflower, a cross was made between EC-734846 × EC734849-II having white and black kernels respectively and another cross was made between EC-734863-II and EC734846 having black striped and white achene colour. The first cross revealed typical monohybrid segregation of 3:1 for black to white seeds. The backcross with recessive parent exhibited 1:1 ratio suggesting achene colour to be single gene controlled with white seed colour controlled by recessive allele. The second cross revealed the ratios of grey seeds with white stripes, greyish black and white seeds with black stripes colour seeded plants to be 9:3:4 suggesting supplementary gene interaction among two genes controlling the seed colour and distinctive markings.

Performance, genetic diversity and inheritance of fruit colour and shape in eggplant (Solanum melongena L.)

Performance, genetic diversity and inheritance of fruit colour and shape in eggplant (Solanum melongena L.)

Flax seed and flower color inheritance is more complicated than once thought

AbstractFlower and seed color are two characteristics of flax (Linum usitatissimum L.) where the mode of inheritance can be obscured by different genetic backgrounds. In this study, eight genetically diverse genotypes of flax with pairwise contrasting seed and flower colors were carefully selected from a large diversity panel and crossed in all directions (56 crosses). The mode of inheritance of seed and flower color was studied in F1, F2, and F3 generations during 2017–2020. Segregation patterns in F1 and F2 generations indicated that seed color is primarily determined by maternal genotype, but parents’ genetic constitution finally defines character expression. Segregation analysis in the F3 generation indicated that seed color is governed by three loci designated as G, D, and Y1. Brown seed color was produced by the recessive allele of the Y1 locus and dominant alleles of the G and D loci (GGDDy1y1). The yellow seed color was attributed to the homozygous condition of two recessive genes and one dominant gene (ggDDy1y1 and GGddy1y1) or the homozygous condition of three dominant genes (GGDDY1Y1). Flower color was found to be controlled by three loci assigned as B1, B2, and C′, independent of the ones for seed color. The blue flower is governed by dominant alleles at all three loci, while homozygous recessive at any of the three loci will result in a white flower. Segregating ratios of 3:1, 9:7, 13:3, and 27:37 blue to white flowers suggested different epistatic interactions between these loci, including duplicate‐recessive and dominant‐recessive states.

Gene exchange and dissection of genetic character associations among Okra

Gene exchange and hybridization of okra [Abelmoschus esculentus (L.) Moench] are important for the development and utilization of new varieties. To study the existing diversity in attributes of okra germplasm and their potential for successful hybridization. The modes of inheritance of leaf shape, stem color, stem pubescence, leaf pubescence, petiole color, leaf color, fruit color, and fruit pubescence were studied to evaluate the possibility of gene exchange among okra varieties. Five okra varieties were characterized using morphological descriptors and parent plants were hybridized in all combinations. The parents, the F1 and F2 plants grown in the field were evaluated for growth and yield-related factors. The qualitative descriptors leaf shape and pubescence, presence, or absence of, anthocyanin in the leaf and stem, and chlorophyll intensity in leaves which are distinct contrasting characters were used for the mode of inheritance. Data collected based on the descriptors were analyzed for variance among parents and hybrid progeny. A chi-squared test was performed to determine the goodness of fit to the expected ratios in the F2 generation. There is significant variation (p<0.05) between parents and their crosses for vegetative growth and yield. All hybrids are elite as they started fruiting early. Most of the crosses performed better than their parents for growth and fruit-related characters. All qualitative characters in the F2 generation were monogenically controlled. Patterns of segregation in the F2 generation was statistically consistent with ratios of 3:1, 1:1 and 1:2:1, indicating that they are controlled by a single gene with complete (3:1 phenotypic ratio) and incomplete (1:2:1 phenotypic ratio) dominance. The gene exchange and mode of inheritance of characters among segregating generations provide information on which selection can be made to improve okra.

Coat color inheritance in American mink

BackgroundUnderstanding the genetic mechanisms underlying coat color inheritance has always been intriguing irrespective of the animal species including American mink (Neogale vison). The study of color inheritance in American mink is imperative since fur color is a deterministic factor for the success of mink industry. However, there have been no studies during the past few decades using in-depth pedigree for analyzing the inheritance pattern of colors in American mink.MethodsIn this study, we analyzed the pedigree of 23,282 mink extending up to 16 generations. All animals that were raised at the Canadian Center for Fur Animal Research (CCFAR) from 2003 to 2021 were used in this study. We utilized the Mendelian ratio and Chi-square test to investigate the inheritance of Dark (9,100), Pastel (5,161), Demi (4,312), and Mahogany (3,358) colors in American mink.ResultsThe Mendelian inheritance ratios of 1:1 and 3:1 indicated heterozygous allelic pairs responsible for all studied colors. Mating sire and dam of the same color resulted in the production of offspring with the same color most of the time.ConclusionOverall, the results suggested that color inheritance was complex and subjected to a high degree of diversity in American mink as the genes responsible for all four colors were found to be heterozygous.

Mapping of the AgPPur gene for the purple petiole in celery (Apium graveolens L.)

Celery (Apium graveolens L.) is a vegetable widely planted and eaten all over the world, and its petiole is the main edible part. To date, there are very few reports about the inheritance and gene cloning of celery petiole color. In this study, based on the purple petiole celery inbred line (ppur) discovered in the early stage of our research, its petiole is purple, and its dwarf stem and leaves are green. The F2 segregation population was configured by crossing it with inbred lines with white petioles. The genetic analysis showed that the purple petiole to white petiole segregation ratio was 3:1 and that the purple trait was dominantly inherited under single gene control. The ppur locus was localized within 324 kb on Chr09 by map cloning, and the gene AgPPur, whose homolog in Arabidopsis encodes the NAC transcription factor, was targeted by RT‒PCR and sequence comparison. Sequencing of both parents revealed a 4-bp deletion at the 5′ UTR in white petiole material, and the gene marker Indel-A4 developed based on this deletion was 100% accurate in both F2 populations. Validation in 30 celery materials with different colored petioles in the laboratory revealed that the marker could perfectly distinguish between purple/nonpurple petiolar materials. Therefore, AgPPur was considered to be the candidate gene controlling purple petioles in celery. Our results could not only improve the efficiency and accuracy of celery breeding but also help to understand the mechanism of anthocyanin synthesis in celery.

Pigment composition analysis of fruit pulp in the recombinant progenies reveals the polygenic nature of pulp color inheritance in guava (Psidium guajava L.)

Pigment composition analysis of fruit pulp in the recombinant progenies reveals the polygenic nature of pulp color inheritance in guava (Psidium guajava L.)

Experimentally testing mate preference in an avian system with unidirectional bill color introgression.

Mating behavior can play a key role in speciation by inhibiting or facilitating gene flow between closely related taxa. Hybrid zones facilitate a direct examination of mating behavior and the traits involved in establishing species barriers. The long-tailed finch (Poephila acuticauda) has two hybridizing subspecies that differ in bill color (red and yellow), and the yellow bill phenotype appears to have introgressed ~350 km eastward following secondary contact. To examine the role of mate choice on bill color introgression, we performed behavioral assays using natural and manipulated bill colors. We found an assortative female mating preference for males of their own subspecies when bill color was not manipulated. However, we did not find this assortative preference in trials based on artificially manipulated bill color. This could suggest that assortative preference is not fixed entirely on bill color and instead may be based on a different trait (e.g., song) or a combination of traits, or alternatively may be due to lower statistical power alongside the bill manipulations being unconvincing to the female choosers. Intriguingly, we find a bias in the inheritance of bill color in captive bred F1 hybrid females. Previous modeling suggests that assortative mate preference and this kind of partial dominance in the underlying genes may together contribute to introgression, making the genetic architecture of bill color in this system a priority for future research.

Inheritance of spike color in einkorn wheat (Triticum monococcum L.)

Aim: specify the spike color inheritance in einkorn wheat (Triticum monococcum L.) hybrids. Methods: reciprocal hybrids between the black-spikeed UA0300282 and white-spikeed UA0300311 cultivated einkorn accessions were created with the use of the “single cross” method. Four generations were analyzed using the segregation analysis method: P1, P2, F1, and F2 at autumn and spring sowing. Results: it was found that for the combination UA0300311 × UA0300282 at autumn sowing, the most suitable inheritance model is MX2-EA-AD, which implies the presence of two main genes with an equal additive effect plus polygene systems with an additive-dominant effect. In the plants of spring sowing, spike color is described by the MX2-CD-AD model, which suggests the presence of two major genes with full dominant effect plus polygenes with additive-dominant effect. In the reciprocal combination UA0300282 × UA0300311, the optimal model that describes best the spike color dispersion in plants of autumn sowing is MX2-ADI-AD, which suggests the presence of two main genes with an additive-dominant-epistatic effect plus polygenes with the additive-dominant effect. Distribution of the spring-sowing plants in terms of the spike color is well described by the MX2-ADI-ADI model – two main genes with an additive-dominant-epistatic effect plus a system of polygenes also with an additive-dominant-epistatic effect. The genes manifest themselves differently in the trait control depending on the weather conditions determined by the sowing time. In the group of direct combination plants (UA0300311 × UA0300282) of autumn sowing, heritability determined by the main gene is 97%, while that determined by polygenes is 2.7%; at spring sowing, these values are 67% and 32% respectively. In the reciprocal combination (UA0300282 × UA0300311) of autumn sowing, the main genes heritability effect is 99%, and the polygenic system accounts for 1%; in plants of spring sowing, respectively, 72%, and 28%. Conclusions: on the basis of the spike color expressiveness in the crossing combination of the einkorn kinds of wheat UA0300311 × UA0300282, the parental forms differ in two main genes and polygenes. The ratio of spike color heritability components depends on the growing conditions: at autumn sowing, 97–99 % of heritability is determined by the main genes, the polygenes account for 1–3 % of phenotypic variability; at spring sowing, the heritability component increases to 28–33 % due to the polygenic complex.

Mapping of the AgWp1 gene for the white petiole in celery (Apium graveolens L.)

Celery (Apium graveolens L.) is one of the most popular leafy vegetables worldwide. The main edible parts of celery are the leaf blade and especially the petiole, which typically has a white, green and red color. To date, there are very few reports about the inheritance and gene cloning of celery petiole color. In this study, bulked segregant analysis-sequencing (BSA-Seq) and fine mapping were conducted to delimit the white petiole (wp1) loci into a 668.5-kb region on Chr04. In this region, AgWp1 is a homolog of a DAG protein in Antirrhinum majus and a MORF9 protein in Arabidopsis, and both proteins are involved in chloroplast development. Sequencing alignment shows that there is a 27-bp insertion in the 3′-utr region in AgWp1 in the white petiole. Gene expression analysis indicated that the expression level of AgWp1 in the green petiole was much higher than that in the white petiole. Further cosegregation revealed that the 27-bp insertion was completely cosegregated with the petiole color in 45 observed celery varieties. Therefore, AgWp1 was considered to be the candidate gene controlling the white petiole in celery. Our results could not only improve the efficiency and accuracy of celery breeding but also help in understanding the mechanism of chlorophyll synthesis and chloroplast development in celery.

Chloroplast genomes and GMOs. History, features and perspectives on plastid transgenesis in plant biotechnology

Chloroplasts provide life on our planet, by creating carbohydrates, amino acids, lipids and O2 through the process of photosynthesis. Two billion years ago, a eukaryotic ancestor entered symbiosis with photosynthetic cyanobacterium, giving rise to chloroplasts. During evolution, chloroplasts transferred most protein-coding genes of free-living bacteria to the nucleus, but retained a semi-autonomous genetic status their own genomes and transcription and translation machinery.&#x0D; The genetics of plastids began in 1909, when Baur and Correns described the non-Mendelian, maternal inheritance of Pelargonium leaf color: green, white and variegated. The presence DNA in chloroplasts (chDNA) was prove in the 1960s, and later have been shown, that male chDNA in zygotes is specifically degraded, assuring maternal inheritance in most plants.&#x0D; The plastid transformation was first described in the unicellular green alga Chlamydomonas reinhardtii [1]. This method of plant biotechnology is an alternative to traditional introduction of foreign DNA into the nucleus, and has a number of advantages: (a) the large copy number of chDNA offers high-level transgene expression; (b) site-specific integration of foreign genes reduces the number of transgenic lines to be evaluated; (c) the lack of gene silencing or position effects in chloroplast genomes; (d) the chloroplast employs a prokaryotic gene expression system, and allows the expression of polycistronic genes [2]. Thus, chloroplasts are becoming attractive hosts for the introduction of new agronomic traits, as well as for the biosynthesis of high-value pharmaceuticals, biomaterials and industrial enzymes.

Volatile Compounds Governed by Single Recessive Gene Impart Aroma in Sponge Gourd (Luffa cylindrica L. Roem).

As a vegetable crop, sponge gourd is widely consumed worldwide due to its health promoting and nutraceutical value. This study describes genetics of an aromatic genotype VRSG-7-17 and deciphers the genetic control and volatile compound composition of sponge gourd. To study the inheritance of this trait, a cross was made between aromatic light-green-fruited VRSG-7-17 and non-aromatic dark-green-fruited VRSG-194 genotypes. The F1s were found to be non-aromatic and have a green fruit colour. Chi-square (χ2) analysis of backcross and F2 population segregating for aroma suggested that the inheritance of aroma in VRSG-7-17 is governed by a single recessive gene in a simple Mendelian fashion. The SPME-GC/MS analysis of the volatile compounds suggested that the compounds responsible for Basmati rice-like aroma were mainly hexanal, 1-octen-3-ol, 3-octanone and limonene. The aroma persists in the cooked VRSG-7-17 fruits, that did not lose fragrance traits at high temperatures. The inheritance of fruit colour was found to be controlled by a single gene with incomplete dominance. The segregation analysis showed that the aroma and fruit colour were not linked, and they segregated independently. The findings will lead to understanding the inheritance of the aromatic compounds in the sponge gourd and may be utilised in the breeding programmes for developing improved aromatic varieties.

Construction of A GBS-Based High-Density Genetic Map and Flower Color-Related Loci Mapping in Grasspea (Lathyrus sativus L.).

Grasspea (Lathyrus sativus L.), a legume crop with excellent resistance to a broad array of environmental stressors, has, to this point, been poorly genetically characterized. High-density genetic linkage maps are critical for draft genome assembly, quantitative trait loci (QTLs) analysis, and gene mining. The lack of a high-density genetic linkage map has limited both genomic studies and selective breeding in grasspea. Here, we developed a high-density genetic linkage map of grasspea using genotyping-by-sequencing (GBS) to sequence 154 grasspea plants, comprising 2 parents and 152 F2 progeny. In all, 307.74 Gb of data was produced, including 2,108,910,938 paired-end reads, as well as 3536 SNPs mapped to seven linkage groups (LG1–LG7). With an average length of 996.52 cM per LG, the overall genetic distance was 6975.68 cM. Both the χ2 test and QTL analysis, based on the Kruskal–Wallis (KW) test and interval mapping (IM) analysis, revealed the monogenic inheritance of flower color in grasspea, with the responsible QTL located between 308.437 cM and 311.346 cM in LG4. The results can aid grasspea genome assembly and accelerate the selective breeding of new grasspea germplasm resources.

Inheritance of fruit colour, fruit ridge pattern and related traits through generation mean analysis in bitter gourd (Momordica charantia L.)

Analysis of generation means in P1, P2, F1, F2, B1 and B2generations of two bitter gourd crosses viz., IIHR Sel-5-8 ×IIHR-144-1 and Arka Harit × IIHR-144-1 were performed todetermine the nature of gene action governing fruit traits.All the parents and generations differed significantly forthe observed traits. For the cross IIHR Sel-5-8 × IIHR-144-1,Chi-square tests in the segregating generations for fruitcolour and fruit ridge pattern were fitted well with theMendelian phenotypic ratio of 3:1. This indicates that greenfruit colour of bitter gourd is governed by single dominantgene and is dominant over white fruit colour. Fruit ridgepattern indicated that the discontinuous ridge of bitter gourdis also governed by monogenic dominant gene and isdominant over continuous ridge. All the quantitative traitsstudied (peduncle length, fruit length and fruit girth) showednon-allelic gene interaction in both the crosses indicatingpresence of epistatic gene action. Nature of gene actionvaried with the character and among crosses and bothadditive and non-additive gene action was involved inexpression of the characters having the duplicate epistasis.This indicates use of reciprocal recurrent selection forimprovement of these traits and also the possibilities ofarriving at better transgressive segregants by selfing thehybrids developed using such selected materials.

Expression of Coat Colour Gene, MC1R in Cattle

Background: Scanty reports are available in the literature about the coat colour inheritance and its related genes in cattle. Therefore, an experiment was conducted to know the expression of coat colour gene, MC1R in cattle. Methods: DNA was extracted from 85 whole blood samples of Red Chittagong cattle (RCC), Non-descriptive deshi and their crossbred of which 75 positive samples were sequenced by Sanger sequencer. Sequence alignment, pair and multi-alignment comparison of the MC1R gene of different genotype and a phylogenic tree constructed by MEGA6 software. Result: RCC has a dominant R gene for red colour. Point mutation was observed at 954 bp and substitution (395G→A) was found in the MC1R gene of RCC genotype. The evolutionary history of branching pattern showed the relatedness of MC1R nucleotide sequences of cattle and this gene have been regulating coat colour inheritance of cattle.