Background: Breastfeeding is an art and a skill which needs to be learnt and mastered. Breastfeeding practice gets affected by many factors like Social, cultural, biophysical, demographic and psychological. One of the important factors for ineffective breastfeeding is the lack of adequate knowledge of women regarding breastfeeding, such as the time of initiation of feeding, feeding of colostrum and mother’s position while feeding the baby. Objective of the study was to find out association of antenatal counselling with the preference for breastfeeding positions, early initiation of breastfeeding and feeding of colostrum. Methods: This was a prospective cohort study where 120 primigravida who had taken structured counseling on breastfeeding during their antenatal period and ended up with vaginal delivery at labour room of SSG Hospital, Baroda were recruited. With every such mother, the next woman who delivers vaginally and has not taken structured counselling during ANC was taken as the control. After delivery, all the mothers were observed for early initiation of breastfeeding, feeding of colostrum and adoption of cross-cradle breastfeeding position in comparison with other positions. Results: The rate of early initiation of breastfeeding (EIBF) and number of mothers who fed colostrum to their babies was significantly higher in case group compared to the control group. The number of mothers preferring the cross cradle position was also higher in case group. Conclusions: Antenatal counseling on breastfeeding helps mothers to raise the rate of early initiation of breastfeeding and feeding of colostrum to babies. It also helped mothers to choose cross cradle position as their preferred method of breast feeding.