Colorimetric Detection Of Hydrogen Peroxide Research Articles

Cr2O3-TiO2-Modified Filter Paper-Based Portable Nanosensors for Optical and Colorimetric Detection of Hydrogen Peroxide.

In the present approach, a Cr2O3–TiO2-modified, portable, and biomimetic nanosensor was designed to meet the requirement of a robust and colorimetric sensing of hydrogen peroxide. Cr2O3–TiO2 nanocomposites prepared via the hydrothermal method were fabricated as a transducer surface on the filter paper using the sol–gel matrix. The color on the filter paper sensor changed from green to blue upon the addition of hydrogen peroxide in the presence of TMB. This change in the color intensity was linear with the concentration of H2O2. RGB software was used as a color analyzing model to evaluate the optical signals. This paper-based colorimetric platform provided us with an improved analytical figure of merit with a linear range of 0.005–100 μM with 0.003 μM limit of detection. The real sample analysis and excellent anti-interference potential results proved the good analytical performance of the proposed design, providing a more promising tool for colorimetric H2O2 detection. Introducing Cr2O3–TiO2 nanocomposite-based paper sensors, being a novel method for optical and colorimetric detection, can pave the way for the development of other sensing devices for the detection of different analytes.

Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide.

Metal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of H2O2 and Br−, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (Br4PR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratiometric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br−) system at wavelengths of 590 and 430, for quantifying H2O2, where the detection limit of the H2O2 is 3.25 μM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. Moreover, through enzyme kinetics monitoring, the Km (H2O2 and NH4Br) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for H2O2 and NH4Br than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future.

Enhanced peroxidase-like activity of copper phosphate modified by hydrophilic phytic-acid and its application in colorimetric detection of hydrogen peroxide

The Cu3(PO4)2·3H2O was modified by hydrophilic phytic-acid (PA/Cu3(PO4)2·3H2O) using a facile co-precipitation method. The peroxidase-like activities of Cu3(PO4)2·3H2O and PA/Cu3(PO4)2·3H2O were demonstrated by catalyzing the peroxidase substrate 3,3,5,5-tetramethylbenzidine in the presence of hydrogen peroxide. The catalytic activity of PA/Cu3(PO4)2·3H2O is about four times than that of Cu3(PO4)2·3H2O, indicating that the peroxidase activity can be enhanced by the introduction of PA. Then, the as-prepared PA/Cu3(PO4)2·3H2O was used to develop a colorimetric method for hydrogen peroxide detection with linear range of 0.1–5.0 mM and detection limit of 0.079 mM. Finally, the applicability of PA/Cu3(PO4)2·3H2O-based assay was validated by the detection of hydrogen peroxide in milk samples, and the recoveries were 92.4%-101.7%. The developed strategy highlights opportunity for the design of inorganic materials with enhanced inherently peroxidase-mimicking performance using PA.

Magnetically separable Fe3O4 NPs/MIL-53(Al) nanocomposite catalyst for intrinsic OPD oxidation and colorimetric hydrogen peroxide detection

Herein, we demonstrate the synthesis of Fe 3 O 4 nanoparticles based metal organic framework MIL-53(Al) nanocomposite [Fe 3 O 4 NPs/MIL-53(Al)] (MIL stands for Material of the Institut Lavoisier) via a facile chemical approach. The fabrication method of nanocomposite is based on the in-situ synthesis of MIL-53(Al) on the surface of pre-synthesized Fe 3 O 4 nanoparticles. The structural features of as-synthesised nanocomposite were elucidated by powder X-ray diffraction (PXRD) analysis, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), optical properties, Fourier transform Infra-Red (FTIR) spectroscopy and thermo gravimetric analysis (TGA). The nanocomposite exhibited excellent catalytic performance towards intrinsic peroxide oxidation of o -phenylenediamine (OPD). Fe 3 O 4 NPs/MIL-53(Al) nanocomposite was also used for the colorimetric detection of hydrogen peroxide and exhibited detection limit of 0.8499 μmol L −1 . Moreover, the nanocomposite was magnetically separable with commercially available magnet and showed significant catalytic stability after six successive cycles.

Three-dimensional MoS2 nanoflowers supported Prussian blue and Au nanoparticles: A peroxidase-mimicking catalyst for the colorimetric detection of hydrogen peroxide and glucose

Well-dispersed Prussian blue (PB) and Au nanoparticles (Au NPs) loaded three dimensional MoS2 nanoflowers (PB-Au@MoS2 NFs) was synthesized by a simple and economical method. The structure, morphology and composition of the hybrid were characterized by XRD, SEM and EDS. Similar to the reported literature, MoS2 nanoflowers showed peroxidase-like activity in catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). This peroxidase-mimicking activity could be enhanced with the introduction of PB and Au NPs. Herein, PB-Au@MoS2 NFs could be used to establish a new platform for the determination of H2O2 and glucose by the chromogenic reaction. Wide linear ranges with 0–15 μM and 0–120 μM for H2O2 and glucose detection were finally obtained. The detection limits were as low as 0.25 μM and 3 μM (with signal to noise ratio of 3), respectively. The established platform was also used successfully for the determination of glucose in human serum and fruit juice samples with excellent sensitivity and stability.

Colorimetric detection of hydrogen peroxide with gadolinium complex of phenylboronic acid functionalized 4,5-diazafluorene

Hydrogen peroxide (H2O2) is a member of reactive oxygen species (ROS) and has substantial physiological roles in living organisms. Currently, advanced techniques facilitate imaging of living systems with gadolinium based contrast agents and intense oxidation regions may be detected with convenient molecular design. Therefore, gadolinium complex of phenylboronic acid functionalized 4,5-diazafluorene (Gd-SCL) was designed for quantitative and colorimetric detection of H2O2. UV–vis, FTIR, 1H NMR, HPLC and ESI-MS methods were used in the characterizations. Conversion of phenylboronic unit to the phenol due to reaction with H2O2 was occurred in 35 min under alkaline aqueous medium with the color change from orange-yellow to transparent colorless and proved by mass analyses. Designed sensor exhibited a good response to H2O2 in the range of 0.5–500 µM with 0.41 μM limit of detection (LOD) in the prepared standard samples. The sensor showed an excellent selectivity for H2O2 in presence of interfering species and the optimum pH range for operation of the sensor was determined in the range of 7–12. Cosmetic product (hair bleach) with 9% peroxide content was used in real sample analyses and LOD was found as 3.83 nM. Considering the magnetic capability of Gd lanthanide, successful H2O2 detection with phenylboronic acid and presuming this research as pre-trial, Gd-SCL complex may be a candidate for magnetic resonance imaging (MRI) agent after further investigations.

Magnetic rod-based metal-organic framework metal composite as multifunctional nanostirrer with adsorptive, peroxidase-like and catalytic properties

Although magnetic stirring is frequently used to enhance the kinetics for adsorption, chemical and biochemical reactions, the introduction of stirrers inevitably leads to the adsorption of analytes and thus interferes with the efficiency of the chemical process or reaction. In this work, magnetic Fe3O4 nanorods with tunable length-to-diameter ratio were synthesized via a hydrothermal method and used as templates for the in-situ depositing of MIL-100(Fe) and gold nanoparticles. Such nanorod-based material can not only function as an adsorbent, nanozyme, and a heterogeneous catalyst for corresponding applications but also serve as a magnetic nanostirrer to enhance kinetics. As a proof-of-concept, the capture of bacteria pathogen, mimic-peroxidase-based colorimetric detection of hydrogen peroxide, and the catalytic reduction of selected organic pollutants were conducted using the as-synthesized Fe3O4@MIL-100(Fe)-Au nanostirrer with and without magnetic field. The results show that the rates of bacteria capture, mimetic enzyme reaction and catalysis were tremendously expedited. We believe this magnetic field-assisted approach holds great promise for future applications, because, not only does it eliminate the use of external magnetic stirrers and thereby decrease the risk of foreign pollution but also, is adaptable for nanoscale reaction systems where conventional stirring is not applicable due to size limitations.

Coral-shaped tin oxide incorporated graphitic carbon nitride nanosheets as peroxidase mimic for sensitive colorimetric and fluorescence quenching based detection of hydrogen peroxide

The enhanced peroxidase-like catalytic activity of coral-shaped graphitic carbon nitride (GCN) incorporated with tin oxide (SnO2) is here reported and applied for the sensitive and selective colorimetric detection of hydrogen peroxide (H2O2). The SnO2/GCN catalyzed the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine, and H2O2 which resulted in the appearance/change of color in the visible range. The results of peroxidase-like activity showed that the growth of SnO2 on GCN nanosheets improved structure, optical, and electronic properties considerably. SnO2/GCN-40% showed the best activity because of the optimal loading of SnO2, unique structural, electronic, optical, and electrical properties. The catalytic reaction of coral-shaped SnO2/GCN-40% followed the typical Michaelis–Menten equation, and the affinity of coral-shaped SnO2/GCN-40% to TMB and H2O2 was higher than that of horseradish peroxide. The present study showed a rapid, selective, and sensitive response toward the H2O2 bioassay in a linear range of 10 − 655 μM with a limit of detection of 0.3 μM (S/N ratio of 3). The study may provide a promising method of performance improvement for applications in catalysis, biosensors, and nanomaterial-engineering fields. Scheme shows the interaction of tin oxide with graphitic carbon nitride which resulted in a coral-shaped structure shown in SEM image. It also shows the interaction of tin oxide and graphitic carbon nitride with H2O2 in the presence of TMB (3,3′, 5,5′-Tetramethylbenzidine) and as a consequence, TMB gets oxidized and ended up in change in color.

Carbon-nitrogen conjugate-composited Cu1.8S with enhanced peroxidase-like activity for the colorimetric detection of hydrogen peroxide and glutathione.

In this work, cystine-glucose Maillard conjugates were composited with Cu1.8S microspheres (Cu1.8S-cgmc) to achieve higher sensitivity for the colorimetric analysis. Enhanced peroxidase-like activity was obtained in Cu1.8S-cgmc with a carbon elemental ratio of 1.83% compared to bare Cu1.8S. The catalytic activity of Cu1.8S-cgmc followed Michaelis-Menten kinetic behavior. The Michaelis-Menten constants of Cu1.8S-cgmc on the substrate was over 2-fold lower than that of the bare Cu1.8S, indicating the higher affinity of Cu1.8S-cgmc. The adsorption equilibrium constant of Cu1.8S-cgmc on the substrate was 9.89-fold higher than that of bare Cu1.8S based on thermodynamic investigations. The conjugated structure and carboxyl, hydroxyl and amino groups on Cu1.8S-cgmc improved its hydrophilicity and adsorption on the substrate. The affinity-improved Cu1.8S-cgmc was applied as a peroxidase mimic in colorimetric detection with promoted sensitivity. Compared to bare Cu1.8S, Cu1.8S-cgmc had a 25.5-fold and 19.8-fold lower LOD for H2O2 and glutathione, respectively. The Cu1.8S-cgmc-based colorimetric method showed excellent sensitivity and accuracy in practical colorimetric detection.

Non-enzymatic colorimetric detection of hydrogen peroxide using a μPAD coupled with a machine learning-based smartphone app.

In the present study, iodide-mediated 3,3',5,5'-tetramethylbenzidine (TMB)-H2O2 reaction system was applied to a microfluidic paper-based analytical device (μPAD) for non-enzymatic colorimetric determination of H2O2. The proposed system is portable and incorporates a μPAD with a machine learning-based smartphone app. A smartphone app called "Hi-perox Sens" capable of image capture, cropping and processing was developed to make the system simple and user-friendly. Briefly, circular μPADs were designed and tested with varying concentrations of H2O2. Following the color change, the images of the μPADs were taken with four different smartphones under seven different illumination conditions. In order to make the system more robust and adaptive against illumination variation and camera optics, the images were first processed for feature extraction and then used to train machine learning classifiers. According to the results, TMB + KI showed the highest classification accuracy (97.8%) with inter-phone repeatability at t = 30 s under versatile illumination and maintained its accuracy for 10 minutes. In addition, the performance of the system was also comparable to two different commercially available H2O2 kits in real samples.

Peroxidase-mimicking Pt nanodots supported on polymerized ionic liquid wrapped multi-walled carbon nanotubes for colorimetric detection of hydrogen peroxide and glucose

Pt nanoparticles have been actively researched as enzyme mimetic nanomaterials for bioanalysis, catalysis and environment treatment over the past years. However, the general issues with individual Pt nanoparticles are stabilization and reproducibility, which lead to the search of Pt based nanocomposite to enhance their activity. In this work, we developed a novel kind of surfactant-free nanocomposites (Pt-PIL-MWCNTs) containing Pt nanodots highly dispersed on polymerized ionic liquid wrapped multi-walled carbon nanotubes, and demonstrated their intrinsic peroxidase-like activity for use in colorimetric detection of hydrogen peroxide and glucose. The Pt-PIL-MWCNTs were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The as-prepared Pt-PIL-MWCNTs were used to catalyze the oxidation of a peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 to the oxidized colored product. Based on the excellent catalytic activity of Pt-PIL-MWCNTs, two colorimetric sensors for detecting H2O2 and glucose, respectively, were constructed with a wide linear range of 10–1000 μM (for H2O2) and 160–900 μM (for glucose), as well as a relative lower limit of detection [5.5 μM (for H2O2) and 50 μM (for glucose)]. The excellent performance of the sensor exhibited that the Pt-PIL-MWCNTs composite could be used as a promising mimetic peroxidase for applications in biosensing.

Cellulose nanofibrils/carbon dots composite nanopapers for the smartphone-based colorimetric detection of hydrogen peroxide and glucose

Fe-doped carbon dots (FeCDs) with high peroxidase (POD)-like activity were prepared by a rapid microwave-assisted method and immobilized on cellulose nanofibrils (CNF) to produce a composite nanopaper. The POD activity of the nanopaper was evaluated by the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and applied to the colorimetric detections of hydrogen peroxide (H2O2) and glucose. CNF acted as a support for the nanozyme, thus imparting it with reusability. It also adsorbed the chromogen during the reaction to induce a color change, thus acting as a test strip for portable on-site detection. A smartphone was employed to monitor this color change which made the whole detection process simple and economical. Under optimal conditions, this method afforded linear ranges of 6–42 μM and 10–70 μM and detection limits of 0.93 and 1.73 μM for the H2O2 and glucose, respectively. The results of the quantification of glucose in human serum samples were comparable to those of the standard glucose oxidase (GOD)–POD method. Further, the nanopaper was potentially reused for ten cycles and presented over a month of shelf life.

2D CTAB-MoSe2 Nanosheets and 0D MoSe2 Quantum Dots: Facile Top-Down Preparations and Their Peroxidase-Like Catalytic Activity for Colorimetric Detection of Hydrogen Peroxide.

We report the facile and economic preparation of two-dimensional (2D) and 0D MoSe2 nanostructures based on systematic and non-toxic top-down strategies. We demonstrate the intrinsic peroxidase-like activity of these MoSe2 nanostructures. The catalytic processes begin with facilitated decomposition of H2O2 by using MoSe2 nanostructures as peroxidase mimetics. In turn, a large amount of generated radicals oxidizes 3,3,5,5-tetramethylbenzidine (TMB) to produce a visible color reaction. The enzymatic kinetics of our MoSe2 nanostructures complies with typical Michaelis–Menten theory. Catalytic kinetics study reveals a ping–pong mechanism. Moreover, the primary radical responsible for the oxidation of TMB was identified to be Ȯ2− by active species-trapping experiments. Based on the peroxidase mimicking property, we developed a new colorimetric method for H2O2 detection by using 2D and 0D MoSe2 nanostructures. It is shown that the colorimetric sensing capability of our MoSe2 catalysts is comparable to other 2D materials-based colorimetric platforms. For instance, the linear range of H2O2 detection is between 10 and 250 μM by using 2D functionalized MoSe2 nanosheets as an artificial enzyme. Our work develops a systematic approach to use 2D materials to construct novel enzyme-free mimetic for a visual assay of H2O2, which has promising prospects in medical diagnosis and food security monitoring.

Photopolymerization-Based Synthesis of Uniform Magnetic Hydrogels and Colorimetric Glucose Detection.

Magnetic hydrogels have been commonly used in biomedical applications. As magnetite nanoparticles (MNPs) exhibit peroxidase enzyme-like activity, magnetic hydrogels have been actively used as signal transducers for biomedical assays. Droplet microfluidics, which uses photoinitiated polymerization, is a preferred method for the synthesis of magnetic hydrogels. However, light absorption by MNPs makes it difficult to obtain fully polymerized and homogeneous magnetic hydrogels through photoinitiated polymerization. Several methods have been reported to address this issue, but few studies have focused on investigating the light absorption properties of photoinitiators. In this study, we developed a simple method for the synthesis of poly(ethylene glycol) (PEG)-based uniform magnetic hydrogels that exploits the high ultraviolet absorption of a photoinitiator. Additionally, we investigated this effect on shape deformation and structural uniformity of the synthesized magnetic hydrogels. Two different photoinitiators, Darocur 1173 and lithium phenyl (2,4,6–trimethylbenzoyl) phosphinate (LAP), with significantly different UV absorption properties were evaluated based on the synthesis of magnetic hydrogels. The magnetic characteristics of the PEG-stabilized MNPs in hydrogels were investigated with a vibrating sample magnetometer. Finally, the colorimetric detection of hydrogen peroxide and glucose was conducted based on the enzyme-like property of MNPs and repeated several times to observe the catalytic activity of the magnetic hydrogels.

Encapsulation of Phosphomolybdate Within Metal–Organic Frameworks with Dual Enzyme-like Activities for Colorimetric Detection of H2O2 and Ascorbic acid

Developing artificial enzyme mimetics for the detection of small biomolecules are a current research interest because natural enzymes bear some serious disadvantages, such as their catalytic activity can be easily inhibited and they can be digested by proteases. Herein, a heteropoly acids (HPA) encapsulating metal–organic framework (MOF) with metal-carbene structure, [Cu10(H3trz)4(Htrz)4][PMo12VO41] (PMA-MOF) as bifunctional enzyme-mimetic catalyst for colorimetric detection of hydrogen peroxide (H2O2) and ascorbic acid (AA) was designed and synthesized. Thanks to the good stability and the synergistic effect of PMA and MOF, PMA-MOF exhibits the lower limit of detection (0.222 μM towards H2O2 and 0.0046 μM to AA), and the smaller Km value (0.0138 mM for H2O2 and 0.136 mM for o-phenylenediamine) compared to most reported MOF- and HPA-based enzyme-mimetic catalyst, to the best our knowledge.

Iron doped graphitic carbon nitride with peroxidase like activity for colorimetric detection of sarcosine and hydrogen peroxide.

The successful synthesis is reported of Mn, Fe, Co, Ni, Cu-doped g-C3N4 nanoflakes via a simple one-step pyrolysis method, respectively. Among them, the Fe-doped g-C3N4 nanoflakes exhibited the highest peroxidase-like activity, which can be used for colorimetric detection of hydrogen peroxide (H2O2) and sarcosine (SA), within the detection ranges of 2-100μM and 10-500 μM and detection limits of 1.8μM and 8.6μM, respectively. The catalytic mechanism of the Fe-doped g-C3N4 nanoflake was also explored and verified the generation of hydroxyl radical (•OH) through fluorescence method. It is believed that the Fe-doped g-C3N4 nanoflakes as enzyme mimics will greatly promote the practical applications in a variety of fields in the future including biomedical science, environmental governance, antibacterial agent, and bioimaging due to their extraordinary catalytic performance and stability. Graphical abstract.

Effect of pyridinium based ionic liquid on the sensing property of Ni0 nanoparticle for the colorimetric detection of hydrogen peroxide

In this work, we presented a new colorimetric technique for the easy and quick detection of hydrogen peroxide. Two types of ionic liquids namely imidazolium and pyridinium containing cations with an acetate anion were evaluated in this study. Ni nanoparticle is synthesized using the wet chemical method, which is further characterized using various analytical techniques. Ni nanoparticles coated with pyridinium based ionic liquid showed robust peroxidase-like activity, which resulted in better dispersion of nickel nanoparticle and is able to quickly catalyze the oxidation reaction of substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), leading to the generation of a blue-colored solution. On the basis of color development, colorimetric sensing of [Pyr]Ac- Ni0 was achieved with a detection limit (LOD) of 120 μM/L within a linear range of 400–4000 μM. The proposed method is significantly selective for [Pyr]Ac- Ni0and can be conveniently and affordably applied to the detection of hydrogen peroxide without any complicated instrumentation.

Microbial fuel cell-assisted biogenic synthesis of gold nanoparticles and its application to energy production and hydrogen peroxide detection

This paper reports the simultaneous synthesis of gold nanoparticles (AuNPs) with a spherical and stable structure using microbial fuel cell (MFC) biofilms. The green, facile, chemical stabilizers and capping-agent free AuNPs synthesis allow the binder-free in situ decoration of AuNPs on MFC anode electrode with the help of special interactions of biofilm. The MFC with AuNPs decorated carbon foam anode electrode produced 62.5% higher (46.37 Wm-3) power density than that of the MFC equipped with plain carbon foam anode (control). The AuNPs facilitated the good adhesion of bacteria, amplified the conductivity, and reduced the internal resistance, resulting in improved overall MFC performance. In addition, the peroxide-mimicking activity was evaluated and the MFC-synthesized AuNPs exhibited significantly higher peroxidase mimicking activity than the chemically synthesized AuNPs, thereby, allowing the easy and rapid colorimetric detection of hydrogen peroxide with a detection limit of 20 uM.

Using G‐Rich Sequence to Enhance the Peroxidase‐Mimicking Activity of DNA‐Cu/Ag Nanoclusters for Rapid Colorimetric Detection of Hydrogen Peroxide and Glucose

AbstractFour kinds of DNA‐based Ag and Cu/Ag nanoclusters (NCs) were synthesized and their fluorescence properties were investigated at pH 7.0 and 5.5. Among them the Cu/Ag NCs templated by DNA containing both C‐rich and G‐rich sequences (the c‐myc TBA−Cu/Ag NCs) give the strongest and the most stable fluorescence emission at ∼555 nm under neutral and acidic conditions. Unexpectedly, the c‐myc TBA−Cu/Ag NCs can catalyze efficiently and rapidly the reaction of hydrogen peroxide (H2O2) with 3,3′,5,5′‐tetramethylbenzidine (TMB) to produce blue TMB oxide (TMBox) under acidic conditions, indicating the high peroxidase‐mimicking activity. Based on this nanozyme the detection for H2O2 and glucose was performed using colorimetric method. The results indicate that the linear range of detection for H2O2 is from 0.1 to 1 mM with the detection limit (LOD) of 7.42 μM, while the linear range is from 0.1 to 0.7 mM and LOD was calculated to be 9.38 μM for glucose. Additionally, glucose can be successfully detected in diluted serum samples and has an acceptable recovery rate, indicating that this nanozyme has a potential application prospect.

Highly sensitive and simple colorimetric assay of hydrogen peroxide and glucose in human serum via the smart synergistic catalytic mechanism.

Due to the own defects of natural enzymes, artificial simulated enzymes are always concerned. Here, the fabricated graphene oxide (GO)/AuNPs nanocomposite exhibits strong synergistic catalysis of peroxidase-mimicking enzymes in combination with the novel property of GO catalytic interface and AuNPs-mediated electron transfer. It can efficiently catalyze the oxidation of enzyme substrate TMB by hydrogen peroxide to form blue TMB oxide. Based on this, the rapid and highly sensitive colorimetric detection of hydrogen peroxide was achieved. Because of the wonderfully synergistic coupling catalysis from GO/AuNPs nanocomposites, the developed artificial enzyme has ultra-strong catalytic activity. For the detection of hydrogen peroxide, the detection limit of this colorimetric analysis is as low as 4.2×10-8M, which is about 1-2 orders of magnitude lower than that of the assays using other single nanoparticles as nanozymes. And it shows high sensitivity. The catalytic oxidation of the prepared nanocomposites to TMB can be completed in minutes, and the response is extremely fast. Combined with the reaction of glucose and glucose oxidase, the colorimetric analysis also realizes the rapid and highly sensitive detection of glucose in human serum. The research results infer that the smart synergy is an effective way to improve the catalytic activity of mimic enzyme. Together with its simplicity in preparation, the GO/AuNPs nanocomposite has excellent development potential in biomedical detection and biosensor design.