Colorimetric Changes Research Articles

SO2-Induced Aging of Hematite- and Cinnabar-Based Tempera Paint Mock-Ups: Influence of Binder Type/Pigment Size and Composition

Hematite- and cinnabar-based paint mock-ups prepared with either rabbit glue or egg yolk binder were artificially aged in an SO2-rich atmosphere, as a model system for investigating the deterioration of tempera paints exposed to an industrial atmosphere. The overall research aim was to identify the type of degradation occurring in tempera paints and the different alteration mechanisms related to the physical, mineralogical and chemical characteristics of the paint. Tempera mock-ups were prepared by mixing binder (egg yolk or rabbit glue) and pigment (cinnabar of different particle sizes or hematite) and were then exposed to SO2 for 2 months in accelerated aging tests. The colour, gloss, reflectance, roughness and micro-texture of the surfaces of the mock-ups were determined before and after the tests. In addition, chemical and mineralogical changes were determined by X-ray Powder Diffraction (XRPD), Attenuated Total Reflection-Fourier Transform Infrared spectroscopy (ATR-FTIR) and Scanning Electron Microscopy with Energy-Dispersive X-ray Spectroscopy (SEM-EDS) analysis. Colorimetric changes were confirmed, mainly in the cinnabar-based paints containing egg yolk, and in the hematite-based paints containing rabbit glue. Neoformed mineral phases have not been detected by XRPD, but precipitation of gypsum on the exposed surfaces has been confirmed by SEM. For cinnabar-based paints, the amount of sulfate-rich deposits was higher on egg yolk mock-ups than on rabbit glue samples, though the opposite was observed for the hematite-based paints. This confirmed the influence of the binder composition and pigment-binder tandem in the susceptibility to SO2 deposition. Pigment particle size did not have a clear influence on the physical and chemical changes in the tempera mock-ups during the ageing tests.

A simple naphthaldehyde based sensor as optical and colorimetric for the detection of Hg2+/Cr3+ in real samples

A new naphthaldehyde based probe 1-((benzylimino)methyl)napthalen-2-ol (NB) synthesized in a single step was designed and characterized by various spectroscopic techniques (NMR, IR, HRMS) and FESEM. The colorimetric and optical sensing properties of the designed probe towards various metal ions were investigated through UV–vis absorption spectroscopy. The synthesized probe showed dual sensing behavior in methanolic solution for Hg2+/Cr3+ ions via colorimetric and spectral changes. Detection limit of the probe for Hg2+/Cr3+ ions was calculated as 2.7 × 10−8 M and 1.1 × 10−8 M respectively. Detection of these ions by the bidentate probe in 1:1 ratio is due to the presence of hydroxyl group and imine group. The synthesized probe works well in pH range 3 to 10. Theoretical calculations also supported the binding mechanism. The synthesized probe was also successfully implemented in different spiked water samples to calculate the percentage recovery. In order to check the practical efficiency, paper strip test was performed for Hg2+/Cr3+ ions detection.

ESIPT-based dual-emissive perimidine derivative as a rapid and sensitive sensor for Cu2+ and Al3+: Construction of memory device, 2-to-1 encoder and 1-to-2 decoder

An ESIPT based fluorescent perimidine derivative oPSDAN was developed and characterized by 1H NMR, 13C NMR and mass spectroscopy. The study of the photo-physical properties of the sensor unveiled its selectivity and sensitivity towards Cu2+ and Al3+ ions. The sensing of ions was accompanied by colorimetric change (for Cu2+) as well as emission turn-off response. The binding stoichiometries of sensor oPSDAN with Cu2+ ion and Al3+ ions were determined to be 2:1 and 1:1, respectively. The binding constants and detection limits for Cu2+ and Al3+ were calculated from the UV–vis and fluorescence titration profiles as, 7.1 × 104 M−1, 1.9 × 104 M−1 and 9.89 nM, 1.5 × 10−8 M, respectively. The mechanism was established by 1H NMR as well as mass titrations and was supported by DFT and TD-DFT calculations. The UV–vis and fluorescence spectral results were further utilized for construction of memory device, encoder and decoder. Sensor-oPSDAN was also tested for determining Cu2+ ions in drinking water.

An Accurate Sensing Technique for Estimation and Detection of Cr(III) in Water by Using Smartphone

This letter demonstrates a sensing technique for rapid detection and accurate quantification of Chromium(III) in water. The principle of the proposed technique is based on change of color of 3-Mercaptopropionic acid functionalized gold nanoparticle upon interaction with Chromium ion. With the increase in Chromium(III) concentration, color changes occur from red wine to blue. The colorimetric changes are captured by a smartphone for detection and quantification. Images are further processed by using MATLAB software and the concentration of Chromium(III) has been estimated. Chromium(III) concentration as low as 1.99 nM with sensitivity of 0.019 AU/nM has been detected successfully. Furthermore, the effects on the sensor response due to the presence of other interfering metal elements have also been studied. The novelty of the system is in its simple design, low-cost, rapid detection, portability, and easy to use.

Visual Assay for Methicillin-Resistant Staphylococcus aureus Based on Rolling Circular Amplification Triggering G-Quadruplex/Hemin DNAzyme Proximity Assembly.

Nowadays, infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have constituted a new challenge for anti-infective treatment. Precise identification and rapid clinical diagnostics of MRSA from other methicillin-sensitive strains entail assays with robust diagnostic efficiency and simple operation steps. Sensitive detection of MecA gene is promising to indicate MRSA infection, but it is challenged by the lack of isothermal and simple strategies. A visual assay based on isothermal rolling circular amplification and G-quadruplex/hemin (G4/hemin) DNAzyme proximity assembly was proposed for the immediate, efficient, and cost-effective detection of MecA in simple operation steps and in a single tube. The presence of MecA specifically drove the formation of circular templates, which further triggered isothermal amplification. The amplified product offered abundant binding sites for DNA-grafted hemin probes to form a novel proximity-assembled G4/hemin DNAzyme structure for colorimetric changing diagnosis. This tandem-repeated novel DNAzyme possessed higher catalytic activity and a lower background signal than traditional G4/hemin DNAzyme, ensuring sensitive discrimination of MRSA (limit of detection: 9.6 pM). Assay stability and antimatrix interference capability enable clinical application, which shows compared diagnostic ability with classic methods (100% sensitivity and 100% specificity) but possesses more simplified procedures and shorter turnaround time (<6 h). This colorimetric strategy in a nonsite-specific and hypersensitive manner holds foreseeable prospects in clinical diagnostic and research applications.

Colorimetric and ratiometric supramolecular AIE fluorescent probe for the on-site monitoring of fipronil.

The overuse of fipronil (FPN, a broad-spectrum insecticide) in agriculture has brought great concerns for environmental pollution and food safety. The development of a rapid, reliable, and portable analytical method for the on-site monitoring of FPN is therefore of great significance but is full of challenge. Herein, a novel supramolecular probe using human serum albumin (HSA) as the host and an aggregation-induced emission-active fluorescence probe LIQ-TPA-TZ as the guest was developed for the colorimetric and ratiometric detection of FPN, displaying fast response (30 s), high sensitivity (LOD ∼ 0.05 μM), and good selectivity and anti-interference performance. Moreover, portable paper-based test strips could be facilely obtained and utilized for the determination of FPN, showing colorimetric changes from yellow to orange. This supramolecular probe also demonstrated great potential in real applications for choosing the best cleaning method to reduce the residue rate of FPN on apples. This study provides a versatile tool for the fast and real-time analysis of FPN, which greatly benefits the on-site determination of pesticides with the use of simple testing apparatus.

A rhodamine based fluorescent and colorimetric chemosensor for the detection of Cr3+ ions and its utility in a molecular logic gate.

A new rhodamine based fluorescent and colorimetric chemosensor S1 was synthesized for the selective recognition of Cr3+, a trivalent metal ion. The interaction of S1 toward different metal ions has been studied via fluorescence and UV-visible spectroscopy. The studies revealed that the fluorescence and colorimetric changes of chemosensor S1 are prominent for Cr3+ over other competitive metal ions. Moreover, the chemosensor S1 exhibits 1 : 1 complex formation with Cr3+ as apparent from the Job's plot and the Benesi-Hildebrand (B-H) plot. Density functional theory (DFT) studies also revealed that the Cr3+ ion is coordinated to three atoms of S1, which validates the formation of a complex between S1 and Cr3+. The limit of detection (LOD) of chemosensor S1 for Cr3+ was 0.21 μM. Furthermore, to explore the recyclability of S1, ethylenediaminetetraacetic acid (EDTA) was added to the S1-Cr3+ solution. On the addition of EDTA to the solution of S1-Cr3+, the reversibility of the complex was observed, and a colorimetric variation was also observed on the addition of Cr3+ and EDTA to S1 which mimics the "INHIBIT "molecular logic gate. Chemosensor S1 also demonstrated practical utility through detection of Cr3+ in the solid state.

A coumarin-based fluorescent chemosensor as a Sn indicator and a fluorescent cellular imaging agent.

In the present study, fluorogenic coumarin-based probes (1-3) through condensation of 4-hydroxy coumarin with malondialdehyde bis(diethyl acetal)/triethyl orthoformate were prepared. The absorption and fluorescence emission properties of 2b and 3 in different solvents were studied, and a considerable solvatochromic effect was observed. The sensitivity of chemosensors 2b and 3 toward various cations and anions was investigated. It was revealed that compound 3 had a distinct selectivity toward Sn2+, possibly via a chelation enhanced quenching mechanism. The fluorescence signal was quenched over the concentration range of 6.6-120 μM, with an LOD value of 3.89 μM. The cytotoxicity evaluation of 3 against breast cancer cell lines demonstrated that the chemosensor was nontoxic and could be used successfully in cellular imaging. The probe responded to tin ions not only via fluorescence quenching, but also through colorimetric signal change. The change in optical properties was observed in ambient conditions and inside living cells.

Direct fluorescence labelling of NO inside plant cells.

Nitric oxide (NO) plays a key role in regulating plant growth, enhances nutrient uptake, and activates disease and stress tolerance mechanisms in most plants. NO is marked as a potential tool for improving the yield and quality of horticultural crop species. Research on NO in plant species can provide an abundance of valuable information regarding this. Hence, we have prepared a simple chemosensor (NPO) for the detection of endogenous NO in chickpea saplings. NPO selectively interacts with NO as determined through a chemodosimetric method to clearly show both the colorimetric and fluorometric changes. After the interaction with NO, the colorless NPO turns yellow as observed by the naked eye and shows bright cyan-blue fluorescence under a UV lamp. The 1 : 1 stoichiometric ratio between NPO and NO is determined from Job's plot resulting in a stable diazeniumdiolate product. The interaction mechanism is well established by absorption, fluorescence titration, NMR titration, HRMS, and DFT calculations. This method has successfully been employed in the plant's root and stem systems to label NO. Confocal microscopy images might help us to understand the endogenous NO generation and the mechanism that happens inside plant tissues.

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens.

Colorimetric readout for the detection of infectious diseases is gaining traction at the point of care/need owing to its ease of analysis and interpretation, and integration potential with highly specific loop-mediated amplification (LAMP) assays. However, coupling colorimetric readout with LAMP is rife with challenges including, rapidity, inter-user variability, colorimetric signal quantification, and user involvement in sequential steps of the LAMP assay, hindering its application. To address these challenges, for the first time, we propose a remotely smartphone-operated automated setup consisting of (i) an additively manufactured microfluidic cartridge, (ii) a portable reflected-light imaging setup with controlled epi-illumination (PRICE) module, and (iii) a control and data analysis module. The microfluidic cartridge facilitates sample collection, lysis, mixing of amplification reagents stored on-chip, and subsequent isothermal heating for initiation of amplification in a novel way by employing tunable elastomeric chambers and auxiliary components (heaters and linear actuators). PRICE offers a new imaging setup that captures the colorimetric change of the amplification media over a plasmonic nanostructured substrate in a controlled and noise-free environment for rapid minute-scale nucleic acid detection. The control and data analysis module employs microprocessors to automate cartridge operation in tandem with the imaging module. The different device components were characterized individually and finally, as a proof of concept, SARS-CoV-2 wild-type RNA was detected with a turnaround time of 13 minutes, showing the device's clinical feasibility. The suggested automated device can be adopted in future iterations for other detection and molecular assays that require sequential fluid handling steps.

A highly effective "naked eye" colorimetric and fluorimetric curcumin-based fluorescent sensor for specific and sensitive detection of H2O2in vivo and in vitro.

Hydrogen peroxide (H2O2) is involved in many important tasks in normal cell metabolism and signaling. However, abnormal levels of H2O2 are associated with the occurrence of several diseases. Therefore, it is important to develop a new method for the detection of H2O2in vivo and in vitro. A turn-off sensor, 2,2-difluoro-4,6-bis(3-methoxy-4-((4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)oxy)styryl)-2H-1,3,2-dioxaborine (DFCB), based on curcumin was developed for the detection of H2O2. The DFCB, an orange-emitting sensor, was constructed by employing 2,2-difluoro-4,6-bis(4-hydroxy-3-methoxystyryl)-2H-1,3,2-dioxaborine (DFC) as the main carrier, and 2-(4-bromomethylphenyl)-4,4,5,5-tetramethyl-1,3,2-doxaborolane as the recognition site. The recognition group on the DFCB sensor could be completely cleaved by H2O2 to generate the intermediate DFC, which would lead to a colorimetric change from bright orange to light blue accompanying by a significantly quenched fluorescence, which could be seen by the naked eye. This sensor exhibited a highly specific fluorescence response to H2O2, in preference to other relevant species, with an excellent anti-interference performance. The sensor DFCB also possessed some advantages including a wide pH response range (6-11), a broad linear range (0-300 μM), and a low detection limit (1.31 μM). The sensing mechanism of the DFCB sensor for H2O2 was verified by HRMS analysis, 1H-NMR titration and DFT calculations. In addition, the use of the DFCB sensor was compatible with the fluorescence imaging of H2O2 in living cells and zebrafish.

Exploring in situ Ni(II) complexation of pyrrole-benzimidazole ligands towards colorimetric sensing of fluoride ion in aqueous medium

Optical sensors with distinct colorimetric output upon binding with fluoride offer a convenient, fast and reliable methodology for its on-site detection. Most of such sensors lose their affinity towards fluoride in aqueous medium, limiting their applicability in real time analysis. In this report, we have been successful in selective, ratiometric and naked eye sensing of F− ion in aqueous medium by a pyrrole-benzimidazole conjugate based receptor R, in presence of Ni2+ salt. It is noteworthy that the receptor R interacts with neither F− nor Ni2+ ions alone in aqueous medium. As studied by UV–Vis spectroscopy and EPR analysis, the sensing mechanism involves F− induced deprotonation of the receptor R, followed by the formation of an in-situ Ni(II) complex, [Ni(II)R2]2−. This complex is responsible for the colorimetric change from dark yellow to tangerine orange. The structure of the complex is further elucidated by DFT calculations. The limit of detection (LOD) is found to be 0.98 ppm.

Selective and sensitive detection of Zn(II) in solution and nanofibers using phosphorescent iridium(III) complexes

Two iridium(III) complexes [(L1)2Ir(tpip)] (Ir1, L1 = 6-phenylnicotinaldehyde), [(L2)2Ir(tpip)] (Ir2, L2 = 6-(4-(trifluoromethyl)phenyl)nicotinaldehyde), bearing tetraphenylimidodiphosphinate (tpip) ancillary ligand, were developed as efficient luminescent sensor for the detection of Zn2+. Ir1 and Ir2 exhibited broad emission bands peaking at 613 nm and 603 nm with PLQY of 0.32 and 0.57 and lifetimes of 1.8 and 1.3 μs, respectively. They showed excellent response to Zn2+ with a high sensitivity and selectivity, low detection limit on the order of 10−7 M and short response time on seconds in solution. Such an outstanding detection was based on significant blue shifts of their emissions. Nanofibers with these complexes were fabricated by electrospinning to realize the on-site detection of Zn2+ in solid-phase with similar colorimetric changes of their phosphorescence as in solution.

A Colorimetric Distinct Color Change Cu(II) 4-{[1-(2,5-dihydroxyphenyl)ethylidene]amino}-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one Chemosensor and its Application as a Paper Test Kit.

In the current research work "4-{[1-(2,5-dihydroxyphenyl)ethylidene]amino}-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one" chemosensor (C1) synthesized by condensation reaction using "4-amino-1,2-dihydro-1,5-dimethyl-2-phenylpyrazol-3-one" and "2,5-dihydroxy actophenone" was used as the effective sensor of metal ion. The C1 shows absorption peak at 326nm due to the C = C bond (π-π*transition), while the absorption peak at 364nm is caused by the C = O bond (n-π*transition). In the presence of copper, C1 only demonstrated a redshift in absorption peak from 364 to 425nm. Even in the presence of other competing metal ions, the hypsochromic shift of the absorption band and the quenching of the fluorescence emission intensity were different for detecting Cu2+, in CH3OH-H2O (v/v = 6:4). The capacity of the C1 to bind with Cu2+ was further proved using DFT simulations. The complex C1 + Cu2+ has a HOMO-LUMO energy gap of 2.8002eV, which is lesser than C1 (2.9991eV) showing improvement in the stability of the C1 + Cu2+ complex. Using the Benesi-Hildebrand and Scatchard plots, calculated Kb values were to be 47,340 and 48369M-1 respectively, showing the creation of stable complexation between Cu2+ and C1 with 1:1 stoichiometry. The limit of detection (LOD) for Cu2+ ion was 649nM. Strip sheets were also built and tested to detect varying amounts of Cu2+ in aqueous solution, and their color change suggested that they might be used for on-site Cu2+ detection in polluted water.

Portable smartphone platform utilizing dual-sensing signals for visual determination of wide concentration ammonium in real samples

Ammonium (NH4+) is crucial for plant growth as well as various industrial processes, the accurate and field measurement of the NH4+ level is of great significance for agricultural precision fertilization and water quality monitoring, but excessive amounts of ammonium can lead to eutrophication of aqueous environments. Herein, the ability of the proposed probe solution and test paper to colorimetric change towards high-concentration NH4+ and ratiometric fluorescence response to low-concentration NH4+ has been developed for visual detection of ammonium in real samples. Specifically, high concentration of NH4+ (0.5–7 mM) could interact with the o-phthalaldehyde (OPA) in presence of K2SO3, resulting in the colorimetric transition from colorless to wine-red with increasing the NH4+ concentration. Subsequently, the addition of the red-emitting CdTe QDs (R-QDs) constructed the OPA/K2SO3/R-QDs ratiometric fluorescent probes for low-concentration NH4+ (less than 1 mM) sensing with a limit of detection (LOD) down to 18 nM, accompanied by a distinct fluorescence color shift from red to blue under UV light. Furthermore, the visualization detection integrated with smartphone was converted to data information (RGB value) through a Color Recognizer APP and successfully used for visual quantitative identification of NH4+. Actually, the proposed probe solution and test paper have been applied to the determination of NH4+ in soil extracts and wastewater samples. Compared with the standard methods, this established strategy can supply stable and reliable detection results, and allow fast sensing at room temperature, exhibiting great application potential for visual on-site detection of NH4+ at different concentration ranges in environmental samples.

Emissive Substoichiometric Covalent Organic Frameworks for Water Sensing and Harvesting.

Emissive covalent organic frameworks (COFs) have recently emerged as next-generation porous materials with attractive properties such as tunable topology, porosity, and inherent photoluminescence. Among the different types of COFs,substoichiometric frameworks (so-called Type III COFs) are especially attractive due to the possibility of not only generating unusual topology and complex pore architectures but also facilitating the introduction of well-defined functional groups at precise locations for desired functions. Herein, the first example of a highly emissive (PLQY 6.8%) substoichiometric 2D-COF (COF-SMU-1) featuring free uncondensed aldehyde groups is reported. In particular, COF-SMU-1 features a dual-pore architecture with an overall bex net topology, tunable emission in various organic solvents, and distinct colorimetric changes in the presence of water. To gain further insights into its photoluminescence properties, the charge transfer, excimer emission, and excited state exciton dynamics of COF-SMU-1 are investigated using femtosecond transient absorption spectroscopy in different organic solvents. Additionally, highly enhanced atmospheric water-harvesting properties of COF-SMU-1 are revealed using FT-IR and water sorption studies.The findings will not only lead to in-depth understanding of structure-property relationships in emissive COFs but also open new opportunities for designing COFs for potential applications in solid-state lighting and water harvesting.

Development of a responsive probe for colorimetric and fluorescent detection of bisulfite in food and animal serum samples in 100% aqueous solution

Bisulfite (HSO3−) has the functions of bleaching, antiseptic, antioxidant, inhibiting bacterial growth, and controlling enzymatic reactions in food. However, long-term consumption of foods containing excessive amounts of bisulfite can be harmful to health. In addition, large doses of sulfur dioxide (SO2) can cause diarrhea, hypotension, allergic and asthmatic reactions in susceptible individuals. Therefore, it is urgent and essential to explore some rapid, reliable, and convenient tools to detect HSO3− in food and SO2 gas. Herein, we exploited a fluorescent probe, NPO, to detect HSO3− in 100 % aqueous solution. The probe has the advantages of easy synthesis, excellent water solubility, significant colorimetric change, good selectivity, high sensitivity, and fast response (within 1 min). Probe NPO was successfully applied for testing strips to visualize the behavior of HSO3− and SO2 gas. Moreover, the probe has been used to monitor the behavior of HSO3− in real food samples and animal serum samples.

Heterogeneous Structural Color Conductive Photonic Organohydrogel Fibers with Alternating Single and Dual Networks.

Intelligent interactive electronic devices can dynamically respond to and visualize various stimuli, promoting the rapid development of flexible electronics. In this paper, an alternating single- and dual-network design strategy was developed for ingeniously constructing an interactive electronic fiber sensor with heterogeneous structural color (HSCEF sensor). The resulting sensor can rapidly output the synchronous electrical and optical dual signals under strain by adjusting the transport distance of conductive ions and the lattice spacing of the photonic crystal (∼200 ms). Meanwhile, the addition of low-freezing-point glycerol endowed the HSCEF sensor with excellent low-temperature tolerance (-25 °C) and cyclic stability. Notably, benefiting from the alternating single- and dual-network structure, the HSCEF sensor exhibits attractive heterogeneous structural color, which achieves colorimetric changes in the full visible light region with high mechanochromic sensitivity (2.25 nm %-1) and large wavelength shift (Δλ ∼ 225 nm). An intelligent wearable interactive sensor is finally used for real-time dynamic detection of joint movements, realizing precise resolution of different amplitudes. This work provides a general strategy to transform conventional photonic gels into heterogeneous structural color ones, and the developed new interactive sensor with rich optical information could be further used for visual health and exercise monitoring, intelligent soft robotics, wearable sensors, etc.

Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba

Acanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve the prognosis of the pathology. However, current diagnosis techniques require time, complex and costly materials making an early diagnosis challenging. Thus, there is a need for fast, accessible, and accurate methods for Acanthamoeba detection by practitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis. Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detection of Acanthamoeba using gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalized with thiolated probes and the presence of target Acanthamoeba genomic sequences, produce a colorimetric change from red to purple. This approach allows the detection of 0.02 and 0.009 μM of the unamplified Acanthamoeba genome by the naked eye in less than 20 min and by color analysis using a smartphone. Additionally, real samples were successfully analyzed showing the potential of the technology considering the lack of point-of-care tools that are mostly needed.

Silver nanoparticles as a nanoprobe for trace level simultaneous detection of streptomycin sulfate and isoniazid and anti-TB activity of their nanoparticles complexes

A facile method was developed for the simultaneous detection of two anti-TB drugs (i.e., streptomycin sulfate and isoniazid) by using maltol capped silver nanoparticles (McAgNPs) as a chemosensor. The interaction between anti-TB drugs and McAgNPs depends on aggregation due to the electrostatic interaction and involvement of an amine group in the formation of a drug nanoparticles complex. The colorimetric change from yellow to pink was observed when McAgNPs interact with streptomycin sulfate (STR.SO4) and leads to the surface plasmon resonance (SPR) quenching at 436 nm followed by a red shift at 664 nm. While interaction of isoniazid (INH) with McAgNPs produced a minor change in color intensity (light to dark yellow) with significant enhancement in SPR at 436 nm. A good linear relationship was observed in terms of drug concentrations (i.e., 0.04–100 and 0.1–100 µM), and the limit of detections (i.e., 0.044 and 0.045 µM) for STR.SO4 and INH, respectively. The proposed method was also validated in environmental, biological and pharmaceutical samples with excellent recovery rates. For practical applicability, the combination of McAgNPs with STR.SO4 and INH were successfully tested against Mycobacterium tuberculosis (MTB) sensitive strain (H37Rv). Obtained results showed that McAgNPs-drug complexes inhibited MTB significantly far below the critical concentrations of the drugs. Hence, McAgNPs not only have great potential as a dual sensor but also their complexes with STR.SO4 and INH for TB treatment.