Substrate Color Research Articles

Masking ability of bleach-shade resin composites using the multilayering technique.

Evaluate the masking ability of bleach-shade resin composites applied by multilayering techniques over colored substrates. Disc-shaped specimens were fabricated from bleach-shade resin composites using different multilayering techniques. Substrates were produced in opaque ceramic discs simulating colored substrates (A2, A3, A3.5, C2, C3, C4) and a bleached tooth (0M1). Color coordinates were obtained using a reflectance spectrophotometer. Color difference was calculated by CIEDE2000 using coordinates of 0M1 ceramic as standard reference. Data were analyzed by one-way ANOVA and Tukey's post-hoc test (p ≤ 0.05). Masking ability interpretation was based on perceptibility and acceptability thresholds. All combinations presented ΔE00 above the acceptable threshold (ΔE00 >1.8), ranging from 2.49 to 7.42, regardless of the restorative system, multilayering strategy or substrate color. Multilayering combinations placed over A2, A3 and A3.5 were moderately (1.8<ΔE00 ≤3.6) and clearly unacceptable (3.6<ΔE00 ≤5.4), combinations associated with C2, C3 and C4 were clearly unacceptable and, extremely unacceptable (ΔE00 >5.4). Lower ΔE00 was observed for combinations with 0.5 mm extra white enamel layer (2.49), or 1.0 mm white dentin layer (2.69), over A2 and A3.5 substrates. Lightness difference showed the greatest contribution to ΔE00 . Bleach-shade resin composite systems applied by the multilayering technique were not able to ensure effective masking ability. Bleach-shade resin composites applied with different combinations of the multilayering technique were not effective in masking colored substrates. Alternative strategies, techniques and dental materials should be explored to obtain acceptable masking.

Low Fluorine Colorless Polyimide Substrate for Flexible OLED Display

Flexible display has become the main stream for the next innovative display. To build the flexible electronics, polyimide varnish is usually coated on the carrier glass with the thickness of a few micrometer and various devices such as flexible solar cell, flexible display, flexible printed circuit board, flexible terahertz sensor, and smart window [1]. Currently available colorless polyimide varnish is usually made of fluorine contained 6-FDA, which has good characteristics such as high transmittance, low yellow index, excellent solubility, but also has bad characteristics such as low decomposition temperature, high coefficient of thermal expansion, high cost, and environmentally unfriendliness due to fluorine. In this study, we make low fluorine contained polyimide varnish by substituting 6-FDA to BPDA + ODPA.Fig. 1 shows the molecular structure of 6-FDA, BPDA, and ODPA. Low fluorine contained polyimide varnish is composed of 40% 6-FDA, 40% BPDA, 20% ODPA and it showed the excellent characteristics such as the glass transition temperature of 300 deg, the decomposition temperature of 366 deg, the yellow index of 2.2, the coefficient of thermal expansion of 20 ppm/deg, and the transmittance at 550 nm wavelength of 98.1%.Because polyimide substrate made from coating polyimide varnish on carrier glass have to be protected using inorganic barrier material such as SiO2 and Si3N4. Also, transparent electrode is also required to apply voltage to the OLED display. Because the color of the polyimide substrate can be changed with the variation of barrier material thickness and transparent electrode thickness, we used optical simulator, Macleod, to access the transmittance change with the thickness change of SiO2 and Si3N4 as shown in fig. 2. Optimal thickness is selected at SiO2 of 400 nm and Si3N4 of 50 nm. The experimental transmittance is well matched with the simulation transmittance as shown in fig. 3.Fabricated device will be detached using innovative laser lift-off (LLO) that utilized excimer laser which scan the interface between the polyimide substrate and the carrier glass from the backside [2]. Fig, 4 shows the laser irradiation and the detached wrinkle free polyimide substrate. Before the delamination, the polyimide substrate is clear, but shows yellowish color during delamination process. Optimum laser energy for the delamination is compared with the color index as shown in fig. 5.We will make the OLED display on the polyimide substrate. Yi et al., Solar Energy 195, 340 (2020),Bian et al., App. Sur. Sci. 499, 143910 (2020). Figure 1

Effect of electroacupuncture at Zusanli (ST36) and Zhongwan (CV12) on intestinal nutritional feeding intolerance in patients with severe acute pancreatitis

To observe the effect of electroacupuncture (EA) of Zusanli(ST36) and Zhongwan (CV12) on intestinal nutritional feeding intolerance in patients with severe acute pancreatitis (SAP). A total of 68 SAP patients (hospitalized from January of 2018 to December of 2019 in Cangzhou Hospital of Integrated Medicine) were randomly divided into control and EA groups (n=34 cases in each group). All patients of the two groups received the same early enteral nutrition treatment through nasojejunal tube. EA (5-15 Hz, 1-5 mA) was applied to bilateral ST36 and CV12 for 20 min, twice a day for 7 days. The incidence of feeding intolerance (abdominal distension, vomiting, diarrhea, constipation, gastrointestinal bleeding), time to reach energy target, intraperitoneal pressure and the number of borborygmus in 1 min were recorded. The contents of plasma high sensitivity -C reactive protein (hs-CRP), IL-6 and endotoxin were measured using Latex immunoturbidimetric method, chemiluminescence and Tachypiens Amebocyte Lysate Azo substrate color development method, respectively, and the contents of urinary lactulose and mannitol detected using high-performance liquid chromatography. The total protein and albumin levels in the blood were measured for assessing the patients' nutrition status, and acute physiology and chronic health evaluation scoring system (APACHE-Ⅱ) score was determined for assessing the severity of disease. Compared with the control group, the incidence of abdominal distension, vomiting and constipation, intolerance rate to feeding, time to reach the energy target, intraperitoneal pressure on day 7, inflammatory indexes, hs-CRP, IL-6, endotoxin, urine L/M on day 4 and 7, and the APACHE Ⅱ score on day 7 were significantly lower (P<0.01), and the number of borborygmus in 1 min on day 4 and 7 after the treatment was significantly higher in the EA group (P<0.01). In comparison with pretreatment, the abdominal pressure and plasma endotoxin level on day 4 and 7, hs-CRP, IL-6 and L/M ratio on day 1, 4 and 7, as well as APACHE Ⅱ score on day 7 after the treatment were significantly decreased in the two groups (P<0.01), and the number of borborygmus on day 4 and 7, and the total protein and albumin on day 7 significantly increased in both the control and EA groups (P<0.01). EA of ST36 and CV12 can shorten the time to reach the energy target, reduce inflammatory response, improve the intestinal mucosal barrier function, and thus reduce the incidence of feeding intolerance in SAP patients.

Highly Skin-Conformal Laser-Induced Graphene-Based Human Motion Monitoring Sensor.

Bio-compatible strain sensors based on elastomeric conductive polymer composites play pivotal roles in human monitoring devices. However, fabricating highly sensitive and skin-like (flexible and stretchable) strain sensors with broad working range is still an enormous challenge. Herein, we report on a novel fabrication technology for building elastomeric conductive skin-like composite by mixing polymer solutions. Our e-skin substrates were fabricated according to the weight of polydimethylsiloxane (PDMS) and photosensitive polyimide (PSPI) solutions, which could control substrate color. An e-skin and 3-D flexible strain sensor was developed with the formation of laser induced graphene (LIG) on the skin-like substrates. For a one-step process, Laser direct writing (LDW) was employed to construct superior durable LIG/PDMS/PSPI composites with a closed-pore porous structure. Graphene sheets of LIG coated on the closed-porous structure constitute a deformable conductive path. The LIG integrated with the closed-porous structure intensifies the deformation of the conductive network when tensile strain is applied, which enhances the sensitivity. Our sensor can efficiently monitor not only energetic human motions but also subtle oscillation and physiological signals for intelligent sound sensing. The skin-like strain sensor showed a perfect combination of ultrawide sensing range (120% strain), large sensitivity (gauge factor of ~380), short response time (90 ms) and recovery time (140 ms), as well as superior stability. Our sensor has great potential for innovative applications in wearable health-monitoring devices, robot tactile systems, and human–machine interface systems.

Partially coupled reaction norms of pupal colour and spot size in a butterfly

Many species cope with heterogeneous environments through adaptive phenotypic plasticity, and plasticity of body colour is a widely used means of minimizing predation risk. Pupal colour in many butterflies is a plastic trait sensitive to pupation substrate, with green and brown pupae primarily formed on leaf and off-leaf substrates respectively. This is thought to be an example of adaptive phenotypic plasticity because the green and brown pupae are better camouflaged against their respective backgrounds. We tested whether pupal ‘spots’—paired circular markings on the anterior abdominal pupal segments of many butterflies—are also phenotypically plastic. Using experiments on the tropical satyrine butterfly Mycalesis mineus, we confirm that pupal spot size is plastic. Because it is known that pupation substrate influences pupal colour in this species, we tested whether substrate also influences pupal spot size. Further, we explored whether there was a correlation between pupal colour and spot size. We tested the effects of pupation substrate and pupal colour on both absolute spot size and spot size relative to body size. Pupal spot size (absolute and relative) was correlated with both pupal colour and pupation substrate. Brown pupae had larger spots, and green pupae formed off-leaf had intermediate sized spots compared to green pupae formed on leaves and brown pupae, the latter of which always formed off-leaf. Thus, the reaction norm of pupal spot size is partially coupled with both pupal colour and pupation substrate in this species. Since pupation substrate in turn influences pupal colour, the correlated traits of pupal colour and pupal spot could comprise a single functional suite, suggesting a developmental link. We speculate that pupal spots may function against predation, but further studies are needed to understand their possible adaptive significance.

Relaxed predation selection on rare morphs ofEnsatinasalamanders (Caudata: Plethodontidae) promotes a polymorphic population in a novel dune sand habitat

AbstractThe ring species Ensatina represents a classic example of locally adapted lineages. The Monterey ensatina (Ensatina eschscholtzii eschscholtzii) is a cryptic subspecies with brown coloration, although a recently discovered polymorphic population within a wind-blown sand region also contains leucistic (pink) and xanthistic (orange) morphs. In the present study, the frequency of leucism/xanthism was mapped across the subspecies’ range, revealing that these morphs are generally rare or absent except within regions containing light-coloured substrate. Attack rates were estimated using clay models of the three morphs, deployed only at the crepuscular period and during the night, on both light and dark substrates at a site within the dune sand region. Model selection found that the interaction between morph and substrate colour best predicted attack rates. Typical morphs had equal attack rates on both substrates while xanthistic and leucistic morphs incurred significantly fewer attacks on light vs. dark substrate, and there was no significant difference in attack rates among morphs on light substrates. These results support the idea that xanthistic and leucistic morphs are poorly adapted for dark substrates compared to typical morphs, but they are more or less equally adapted for light substrates. We suggest that this microgeographic island of relaxed selection on light-coloured morphs helps to explain the existence of this polymorphic population.

Interactive effects of light and substrate colour on the recruitment of marine invertebrates on artificial materials

Marine invertebrate larvae are highly phototactic during early stages of larval period and greatly influenced by light, colour and other physio-chemical properties of the substrates. In the present study, the effects of light and substrate colour on recruitment of marine invertebrates were investigated. Four different colour panels (red, green, blue and yellow acrylic sheets) and colourless panel (clear transparent acrylic sheet) were suspended at 2 m depth in the central Red Sea for 4 weeks during two seasons (spring and summer). The effects of substrate colour on invertebrate recruitment were tested under light and shade conditions. The results revealed significant variations in the recruitment of invertebrates between different colour panels and the panels exposed to light and shade. Higher densities of invertebrates were observed on the panels exposed to shade but the bryozoans abundance was high on the panels exposed to light. Tube worms and bryozoans preferred blue colour panels and the abundance of barnacles and bivalves were high on red colour panels. Significant interaction between substrate colour and light was observed in the abundance of barnacles, tube worms, bivalves and bryozoans. In conclusion, this study indicated the ability of some marine invertebrate larvae to discriminate coloured substrates depending on the light intensity.

The relationship between body and substrate color for Ambystoma altamirani (Caudata: Ambystomatidae) from the Arroyo los Axolotes, Mexico

The relationship between body and substrate color for Ambystoma altamirani (Caudata: Ambystomatidae) from the Arroyo los Axolotes, Mexico. To determine whether the coloration of Ambystoma altamirani varies and whether the color is related to the color of the salamander’s substrate, A. altamirani from the Arroyo los Axolotes, state of México, Mexico, was examined. The study was conducted from February 2018 to January 2019, and the colors classified by comparison with a standard color wheel. The most common base color was olive-green (64%) followed by black (21%). The most common color combinations were olive-green with black markings (44%), solid black (14%), and olive-green with black and yellow markings (11%). Olive-green salamanders were present in every month of the study, whereas the other base colors were found in fewer months, with the greatest diversity of base colors being observed from April to July. Olive-green, black, and “light” A. altamirani were found on dark substrates more frequently than expected based on availability compared to intermediate or light substrates. Because there is no evidence for substrate color matching, substrate selection likely is based on other environmental features.

Does substrate colour affect the visual appearance of gilded medieval sculptures? Part II: SEM\u2013EDX observations on gold leaf samples taken from medieval wooden sculptures

In the previous paper (Part I), the colorimetry and interferometric microscopy measurements on modern gold leaf models have revealed that the visual appearance of a gilded surface, both burnished and unburnished, depends strongly on the substrate type, surface roughness and texture, but not on the colour of the substrate. In this second part, we investigate the materials compositions and technical specifications of medieval gold leaf through combining literature sources and materials analysis such as scanning electron microscopy coupled with energy dispersive X-ray analysis (SEM–EDX) on samples taken from gilded wooden sculptures. Our study shows that the late medieval gold leaf has a high purity of about 23.7 carat and has an average thickness of 160 nm (with a peak value of 138 nm), purer and thicker than the modern gold leaves studies in Part I. Supportive Rutherford backscattering spectrometry (RBS) measurements on gilded models confirms the accuracy and reliability of the SEM–EDX observations on the medieval gold leaf samples. We additionally present observations of a rarely recorded special variant of medieval gold leaf—“fine reinforced gold leaf”. Combined with the findings from Part I, we conclude that light penetrating the medieval gold leaf and reflected from the gilding substrate could not be a significant, or even perceptible contribution to the visual appearance of the gilding. We argue that the misconception surrounding the correlation between the substrate colour and the gilded surface appearance can be attributed to the historical development of gilding and polychromy technologies.

Does substrate colour affect the visual appearance of gilded medieval sculptures? Part I: colorimetry and interferometric microscopy of gilded models

In the history of medieval gilding, a common view has been circulated for centuries that the substrate colour can influence the visual appearance of a gilded surface. In order to fully understand the correlation between the gilding substrate and the colour appearance of the gold leaf laid above, in this paper (Part I) analytical techniques such as colorimetry and interferometric microscopy are implemented on models made from modern gold leaves. This study demonstrates that the substrate colour is not perceptible for gold leaf of at least 100 nm thickness, however the surface burnishing can greatly alter the visual appearance of a gold surface, and the quality of the burnishing is dependent on the substrate materials. Additionally, surface roughness and texture of the substrate can play supplementary roles, which can be visually observed through digital microscopy and quantified through interferometric microscopy. The findings in this paper will form the basis for the study of gold leaf samples taken from medieval European gilded sculptures in Part II.

Multi-color modulation of phase-changing optical coatings

Now, various display screens transmit massive information to people all the time and become an important window of human-computer interaction. Solid state optical coating utilizes optical interference effect and inherent optical properties of materials to achieve multiple colors. In this paper, between Fabry-Perot (F-P) cavity coating with dielectric layer and strong interference effect (SIE) coating without dielectric layer were compared. Effect of film thickness on F-P cavity optical phase change coating was studied. The results show that the F-P cavity coating with a dielectric layer has more reflective states than the coating without a strong interference effect, that is, potential for optical coating display. In addition, in the F-P cavity coating, by controlling the thickness of SiO2 and Ge2Sb2Te5 (GST) film, the optical coating shows rich color due to the change of F-P optical cavity length and strong interference effect of materials. We also carried out a study of the flexible substrate compatibility and substrate color of the two structures, and found that they are well compatible with flexible materials, and F-P is more sensitive to substrate color than SIE structures in terms of substrate color. Due to the far subwavelength layer thickness, most phase shift is accumulated on reflection at the interfaces, which makes the reflectance profile robust under tilted incidence.

Correlation between substrate selection and body color of neotropical katydids (Orthoptera: Tettigoniidae)

Leaf mimesis is the most common form of predator avoidance displayed by neotropical katydids. Such camouflage is expressed through various body and colour forms to resemble different leaf shapes within the rainforest. The objective of this work was to determine if roosting katydids select vegetation the same colour as their body during the day, and if this pattern differs in the nighttime. I sampled katydids from the Monteverde Cloud Forest (Puntarenas, Costa Rica) by hand capture during the night and released them during the day, noting behaviour and final roosting vegetation. In total, I collected 61 katydids consisting of two monomorphic genera (Idiarthon, n = 16, Lophaspis, n = 8), and two polymorphic genera (Mimetica, n = 12, Orophus, n = 25). I generated contingency tables of counts for monomorphic and polymorphic katydid genera, comparing body colour to daytime and nighttime roosting substrate colour, and vegetation type. I then conducted chi‐squared goodness‐of‐fit tests on the contingency tables. My results suggest that during the day, both monomorphic and polymorphic species select vegetation which matches their body, however, this pattern is absent during the nighttime. However, species within the leaf‐mimicking genus Mimetica displayed no colour‐matching behaviours during the day. This study is the first quantitative analysis of behavioural colour‐matching of neotropical katydids and provides useful insight into katydid functional coloration and evolution.

Fabrication of biosensors by bacterial printing on different carriers using a laser printer

In this study, a commercial laser printer was used for the first time to print bacteria on different substrates as bacterial carriers to be used in biosensors. For this purpose, the spores of Geobacillus stearothermophilus and Bacillus atrophaeus were mixed with the printer toner and used as a bio-powder in a conventional laser printer to print on a polypropylene film (PF) or copy paper (CP), transfer paper (TP), and filter paper (FP).‏‏ Subsequently, the effects of the substrate material, print density, print pattern, and color intensity were investigated on the number of bacteria recovered. It was also found that FP was the best substrate, followed, with a significant difference, by TP, for the maximum number of bacteria recovered. Moreover, a color intensity of 100% was found the most appropriate. However, changing print density and pattern showed differences in their effects on bacterial numbers for the two strains tested albeit the differences were not significant. ‏‏Based on the results obtained, it may be claimed that laser printers can be effectively used for transferring bacteria to paper substrates and used in fabricating biosensors. Printing capabilities and novel toner bio-powders are exploited to choose the proper number of printed bacteria to adapt the printed substrates for use in manufacturing bacterial biosensors.

Influence of colour on oviposition behaviour in green lacewing Chrysoperla zastrowi sillemi (Esben - Petersen) (Neuroptera: Chrysopidae)

The Green lacewing, Chrysoperla zastrowi silleni (Esben-Peterson), also known as “aphidlion” is a beneficial insect predator of various insect pests. The laboratory experiment was conducted during 2018-19 to evaluate the substrate colour preference for egg laying by C. zastrowi sillemi. Egg receiving sheets were pasted with white, black, green, indigo blue, yellow, brown, violet, sky blue, pink, red and orange colour papers. Among all colours C. z. sillemi females preferred orange colour as a substrate for egg laying with maximum number of eggs (43.13/female/day) followed by red colour substrate (25.50 eggs/female/day). White and black were least preferred.

Spectrophotometric examination of the optical effects of monolithic multilayered zirconia with different substrates

ABSTRACT The goal of this study is to examine specific optical effects of multilayered and coloured monolithic zirconia considering thickness and substrates of different colours using one of the most advanced spectrophotometers of the world. Multilayered zirconia specimens were used for the study with the thickness range of 0.5–2.5 mm and six types of substrate materials and three types of metal substrates. Measurements were carried out at Budapest Technical University with a PerkinElmer®Lambda1050UV/Vis/NIR spectrophotometer. The substrate colour and the thickness of zirconia affects the optical results, with special regard to colour perceptibility and acceptability. Monochromatic and multilayer zirconia show both similarities and discrepancies in behaviour, i.e. spectral reflectance and ΔE. Owing to the multi-coloured characteristics of multilayered zirconia the optical effect of the substrate less predictable than in the case of monochromatic zirconia thus requiring more detailed planning and implementation.

Detection of MicroRNAs by In Situ Hybridization in Skin.

MicroRNAs (miRNAs) are a family of small noncoding RNAs (~19-24nt) playing a key role in the execution of gene expression programs in various cells and tissues. Many technical challenges have been encountered when investigating miRNAs, in particular, determining the spatiotemporal expression pattern of miRNAs in cells and tissues. We describe here a well-established in situ hybridization protocol for the detection and analysis of spatiotemporal expression patterns of miRNAs in skin and its appendages such as the hair follicle in both frozen and paraffin-embedded tissue sections. We describe in detail the different steps that are associated with utilizing in situ hybridization procedure on either frozen or paraffin-embedded tissues for miRNAs localization. Postfixation, tissues are hybridized with LNA double labeled probes with digoxygenin. Detection of hybridized probes is performed by using an alkaline phosphatase coupled antibody against digoxygenin. The final step involves the use of substrates to develop the color of alkaline phosphatase-LNA-probe structure leading to identification of the spatiotemporal location of target miRNAs in target tissue and cells. We also discuss two options for substrate color development in these procedures: (1) NBT/BCIP and (2) BM Purple. This method is a simple and convenient way of determining the spatiotemporal expression pattern of miRNAs, which has been a challenge since their discovery, due to their relatively small size. Knowledge gained from in situ hybridization is crucial for better understanding of the roles of individual miRNA(s) during distinct stages of development in various cells and tissues. These protocols will be beneficial to the wider scientific community.

Effect of background color and shelters on female pigmentation in the ornamental red cherry shrimp Neocaridina davidi (Caridea, Atyidae)

AbstractNeocaridina davidi, the “red cherry” shrimp, is becoming popular in trade markets because of the reddish coloration of females. The aim of this study was to analyze the impact of background substrate color, and the presence of shelters on N. davidi female pigmentation and astaxanthin content. In the first experiment juveniles were assigned to one of three treatments: white, red, or black background substrate. After 90 days, females exposed to the black background showed higher astaxanthin content and relative carapace colored area than those exposed to white and red backgrounds. The second experiment evaluated the presence of shelters with white or black backgrounds. Juveniles were assigned to one of four treatments: white background with shelters (WS), white background without shelters (WWS), black background with shelters (BS), and black background without shelters (BWS). After 90 days the presence of shelters did not influence either the color nor total astaxanthin content. Females exposed to BWS and BS had higher astaxanthin content and relative carapace colored area than those exposed to WWS and WS. Growing N. davidi with black substrates could be a low cost and easy method to improve its economic value. Furthermore, this species could be cultivated as a pigment‐enriched food source for other aquatic species.

Recruitment patterns of the solitary ascidian Phallusia nigra Savigny, 1816 on artificial substrates submerged in the central Red Sea, Saudi Arabia

Abstract The solitary ascidian Phallusia nigra is commonly found on hard substrates along the Jeddah coastal waters of the central Red Sea. In this study, the recruitment pattern of P. nigra on artificial substrates was assessed in relation to their type, surface color and orientation. The results showed a higher recruitment rate of the ascidian species on concrete and dark panels. The abundance of the ascidian on test panels varied between the four seasons. The orientation (vertical or horizontal) of the panels did not show any major difference in the recruitment. Significant effects of light intensityon the recruitment of P. nigra were observed on test panels, with higher abundance on panels submerged in the shade. In conclusion, this study clearly indicates the importance of the type and color of substrates in the recruitment of ascidians on artificial materials.

Paper-based colorimetric probe for highly sensitive detection of folic acid based on open-ring form amplification of rhodamine B derivative

A simple colorimetric method was proposed for the determination of folic acid (FA) using a paper-based substrate. The mechanism of the folic acid probe is based on the improvement of electron transfer from the rhodamine B derivative to Cu2+ in the presence of folic acid. Upon the addition of folic acid, the structural conversion of the rhodamine B derivative, in which the spirolactam ring (colorless) was opened little to the open-ring form (pink color) by Cu2+, was amplified clearly. The change of color intensity can be observed by either UV–vis spectrophotometer or naked eye. The color of the paper-based substrate was analyzed using the red, green, blue (RGB) values, which were collected from images captured with a smartphone application. The folic acid paper-based probe exhibited good linearity in the range of 40–260nM with a limit of detection (LOD) of 22nM. The proposed probe exhibits a high potential for detecting folic acid in real samples including drug tablets, human serum, and urine.

Evaluation of the effectiveness of cryptic coloration of the Carolina anole’s skin

Cryptic coloration of animals’ integument is one of the effective adaptations that allow them to lead an active lifestyle while being protected from natural enemies due to visual disguise. This is achieved by the similarity of body color of a particular individual to the background of various substrates in its environment. The morphological and functional basis of cryptic coloration in vertebrates, including reptiles, is ensured by the skin pigmentation. Using bioinformatic methods, we calculated the skin camouflage index of the Carolina anole (Anolis carolinensis Voigt, 1832) in various conditions of its habitat. The skin camouflage index (Ic) is the ratio of the sum of the average values of rgb coordinates of the skin color to the sum of the average values of rgb coordinates of the color of the external substrate. Ic satisfies the effective level of adaptation to habitat conditions if it falls within the range of 0.80–1.20. It has been shown that rgb-values of the dominant color of the dorsal skin of green anoles slightly differ from the similar parameters characteristic of the deciduous habitat, which is reflected by Ic of its skin (0.94). In the brown anoles on a background of woody substrate, the Ic value of its skin (0.88) is also optimal, since it exceeds the lower limit (0.80), which indicates close values of the sums of the rgb coordinates of the skin color and the color of the external background. In the mixed green-brown anoles, the deciduous habitat is preferable to the woody one. In the first case, Ic (1.11) is in the optimum zone, and in the second case, Ic (0.70) goes beyond the lower limit of the optimum, which indicates a greater vulnerability of the animal to external threats. We have confirmed the relativity of visual hiding of the skin that is effective only in the habitat conditions in which the camouflage abilities of the skin manifest as fully as possible.