PurposeAcquiring high-quality ultrasound images of deep abdominal organs and vasculatures in obese adults (BMI >30 kg/cm2) is considered challenging. The aim of the study was to assess the inter-transducer variability in B-mode and color Doppler image quality from four commercial ultrasound transducers through qualitative and quantitative analyses. MethodsFour curvilinear transducers on three ultrasound scanners were used to acquire B-mode and color Doppler images of deep abdominal structures in 15 obesity ≥ class II (BMI >35 kg/cm2) adults. Using visual-qualitative assessment and an offline image processing software, visual-qualitative score and quantitative mean pixel values of B-mode images, and color area ratios of color Doppler images were calculated. Differences in these values among the transducers were analyzed using one-way ANOVA. The intra- and inter-observer reliability of visual-qualitative assessment and offline image processing was tested using the intraclass correlation coefficient (ICC). ResultsDifferences in visual-qualitative score, mean pixel value of B-mode images, and color area ratio of color Doppler images among the four transducers were significant (p < 0.001). Transducer −4 produced the highest quality of B-mode (45–53% improvement) and color Doppler (22–73% improvement) images among the transducers. Intra-observer repeatability and inter-observer reproducibility were higher with performing offline image processing than visual-qualitative assessment (ICC: 0.97–0.99 versus ICC: 0.76–0.97). ConclusionThere was significant image quality variability between different transducers. Transducer −4, a transducer designed specifically for high BMI patients, had the highest quality B-mode and color Doppler images compared to the other transducers lending to improved ultrasonographic visualization in obese patients.