Color Change Research Articles

Dielectric Barrier Discharge Cold Plasma Improves Storage Stability in Paddy Rice by Activating the Phenylpropanoid Biosynthesis Pathway.

A nonthermal pretreatment using dielectric barrier discharge cold plasma (DBD-CP) was developed to improve the stress resistance of paddy rice during postharvest storage. The physicochemical properties, bioactive characteristics, and secondary metabolites of paddy rice were assessed after applying an optimized DBD-CP procedure, with enzyme activities and gene expression monitored over a 60 day storage period at 35 °C. A 17.06% reduction in the total color change index was noted in the DBD-CP group. Bioactive compounds, particularly gallic acid, were significantly increased, enhancing the defense mechanisms against high-temperature stress. Nontargeted metabolomics analysis indicated an upregulation of phenylpropanoid metabolism in DBD-CP-treated rice compared to controls, with notable increases in secondary metabolites such as coumaric acid, caffeic acid, and sinapic acid, suggesting potential biomarkers for stress resistance. Further verification showed significant enhancements in key enzymes of phenylpropanoid metabolism, including phenylalanine ammonia lyase (PAL), cinnamic acid-4-hydroxylase (C4H), plant coumaric acid-3-hydroxylase (C3H), and cinnamyl alcohol dehydrogenase (CAD), with increases ranging from 1.71 to 2.28 times. Gene expression levels of OsPAL7, OsC4H4, and OsCAD2 aligned with these enzymatic changes post-DBD-CP treatment. In conclusion, DBD-CP treatment can modulate phenylpropanoid metabolism in paddy rice, thereby enhancing bioactive compound levels to reduce stress damage during high-temperature storage.

Mycological analysis in three schools over four seasons using passive air sedimentation

The aim of the study is the isolation and identification of fungi using passive air sedimentation. This study analyzed 540 mycological samples from three primary schools in Zenica, collected in September, December, February, and May. Each season, 135 samples were taken from five rooms (two classrooms, a gym, a locker room, and a library) in each school. Samples were collected three times daily at three different heights with 15-minute exposure times. Samples were refrigerated and transported in sterile bags, incubated for 24 hours, and inoculated on specific agars with and without additives. Plates were incubated at 37°C and 25°C for up to 7 days, followed by examinations. Petri dishes were used for passive air sampling, and colonies were counted after incubation. The average number of microorganisms (CFU/m³) was calculated using Omeliansky’s method. Statistical methods included the Chi-squared test and p-value. Colony appearance was assessed visually and microscopically using a light microscope. Growth rate, size, structure, and color changes were monitored. In September, the highest mold concentrations were at H. Kikić Primary School (796 CFU/m³, not significant), M. Dizdar Primary School (1260 CFU/m³, not significant), and A. Šantić Primary School (3980 CFU/m³, significant). Penicillium spp. and Alternaria spp. were most prevalent, with Alternaria spp. significant at H. Kikić Primary School. In December, the highest mold/yeast concentrations were at H. Kikić Primary School (4578 CFU/m³, not significant), M. Dizdar Primary School (1924 CFU/m³, significant), and A. Šantić Primary School (2587 CFU/m³, not significant). Penicillium spp. was most prevalent. In February, the highest mold concentrations were at H. Kikić Primary School (4578 CFU/m³, not significant), M. Dizdar Primary School (2786 CFU/m³, not significant), and A. Šantić Primary School (5838 CFU/m³, significant). Aspergillus spp. and Penicillium spp. were equally prevalent. In May, the highest mold/yeast concentrations were at H. Kikić Primary School (6568 CFU/m³, significant), M. Dizdar Primary School (3516 CFU/m³, significant), and A. Šantić Primary School (7431 CFU/m³, significant). Aspergillus spp. was most prevalent. These findings highlight the importance of regular monitoring and implementing appropriate ventilation measures to manage air quality and health concerns in schools.

The effect of diode 980 nm laser-activated bleaching on surface roughness and color change in composite resin restorations (in vitro study)

The effect of diode 980 nm laser-activated bleaching on surface roughness and color change in composite resin restorations (in vitro study)

A Wide‐Temperature Adaptive Electrochromic Device Based on a Poly(vinyl alcohol)/Poly(acrylic acid) Gel Electrolyte

AbstractAs a promising energy‐saving technology, electrochromic technology has been widely investigated for practical applications. However, there is relatively few research about the applications under certain extreme climatic conditions since gel electrolytes often occur freezing and volatilizing. In this work, a poly(vinyl alcohol)/poly(acrylic acid) (PVA/PAA) gel electrolyte with good anti‐freezing and heat‐resistance performance is developed. Utilizing hydrated tungsten oxide (WO3·xH2O) and polyaniline (PANI) as electrochromic materials and the PVA/PAA gel as electrolyte, a WO3·xH2O/PVA/PAA‐ethylene glycol (EG)‐H2O/PANI electrochromic device (WO3·xH2O/PAEH/PANI ECD) is obtained, which can work efficiently at wide operating temperatures from −20 to 60 °C. The device has multiple reversible color changes, a large optical modulation of 66.2% at 600 nm, high coloration efficiency of 386.0 cm2 C−1, fast responses (with coloration/bleaching times of 1.3/1.1 s), as well as excellent cyclic stability (82.0% of the initial optical modulation is still retained after 2100 cycles). This work reveals the potential application prospects of PAEH gel electrolyte in practical extreme operating conditions.

Advanced Intelligent Anti‐Counterfeiting in a Memory Storage Phosphor Through Response of Charge Carriers to Temperature

AbstractPersistent phosphor as a printing anti‐counterfeiting material has attracted strong attention owing to its inherent luminescent decay process and multi‐mode luminescence characteristics under external field stimulation. However, the dynamic persistent luminescence (PersL) pattern of a single material is only manifested in color changes and still faces the problem of being easily decrypted. Herein, a multi‐color luminescent material with a wide range distribution of traps is developed, which can emit strong white PersL. In addition, the phosphors show an intelligent response of thermo‐stimulated luminescence to ambient temperature via recalling the charging temperature of the traps. The PersL mechanism is proposed based on electron transfer between PbO6 and TbO6 octahedrons through the O bridging. Based on the intelligent response of thermo‐stimulated luminescence to temperature, high‐throughput multi‐channel luminescence anti‐counterfeiting can be achieved on a single phosphor recording layer. This study not only provides further insights into the PersL mechanism, but also opens a new door for constructing intelligent responsive anti‐counterfeiting materials.

Gamma ray irradiation on Swiss cheese estates (Monstera adansonii): growth, development, and variation

Purpose Swiss Cheese (Monstera adansonii) is an ornamental plant valued for its exotic leaves with openings and for the variety of colors. The technique of controlled exposure to gamma radiation was investigated to induce variegation (color change) in the leaves of this plant. Materials and methods Monstera adansonii cuttings were irradiated with different doses of radiation with 60Co gamma rays (0, 1, 5, 10, 15, and 20 Gy) and evaluated for size, color, health, and growth. Results Cuttings irradiated with 1 and 5 Gy exhibited temporary variegation in leaf color, but did not maintain these characteristics over time. Cuttings with higher doses of radiation (10, 15, and 20 Gy) did not survive well and showed a reduction in growth, number of leaves, health, and sprouting rate. Conclusions This research concludes that gamma radiation can affect the development of cuttings and shows the potential to induce variegation at lower doses, but more studies and prolonged observation are needed to determine whether this technique can produce variegation in a consistent and lasting way in M. adansonii. Therefore, although promising initial results have been observed, it is premature to state that gamma radiation is an effective method for inducing variegation in this plant.

CRISPR/Cas9-Mediated Genome Editing of T4 Bacteriophage for High-Throughput Antimicrobial Susceptibility Testing.

The accurate and effective determination of antimicrobial resistance is essential to limiting the spread of infectious diseases and ensuring human health. Herein, a simple, accurate, and high-throughput phage-based colorimetric sensing strategy was developed for antimicrobial susceptibility testing (AST). Taking advantage of the CRISPR/Cas9 system, the genome of the T4 phage was modularly engineered to carry lacZ-α (lacZa), a marker gene encoding the α-fragment of β-galactosidase (β-gal). T4lacZa phages were identified by blue-white selection and then used for a biosensing application. In this strategy, the bacterial solution is exposed to the T4lacZa phage, causing target bacteria to overexpress β-gal. Upon the addition of a colorimetric substrate, the β-gal initiates an enzymatic reaction, resulting in a solution color change from yellow to red. This sensing strategy offers a visual way to monitor bacterial growth in the presence of antibiotics, enabling the determination of bacterial antimicrobial susceptibility. As a proof of concept, our developed sensing strategy was successfully applied to identify 9 different multidrug-resistant Escherichia coli (E. coli) in urine samples with 100% specificity. Compared with conventional disk diffusion susceptibility tests, the engineered phage-based sensing strategy can shorten the detection time by at least half without losing detection sensitivity, providing an alternative high-throughput method for AST in clinical diagnosis.

Role of Chitosan in the Coloring of Berries and Phytochemical Changes in Physalis angulata L. During Harvest Maturity

Physalis angulata L. berries are green in the initial stage of maturity and turn yellow when fully mature due to an increase in carotenoids. However, the coloration of the epidermis of the berry should not be the reference for harvesting maturity, since the calyx is fully closed. The use of an edible chitosan coating delays the color changes in the berry by reducing the respiration rate. This work studies the effect of harvest maturity and chitosan on the shelf life and berry coloring in Physalis angulata L. Three factors were considered, being harvest maturity stage (green mature (GM), yellowish-green (YG) and yellow (Y)), chitosan coating (0, 0.5, and 1%), and storage time (10, 20, 30 days). The fruits were stored at 20 °C and 85% Rh. The results showed that on the harvesting stage, chitosan coating had a significant effect on the quality and shelf life of berries during storage time. The value of carotenoid and phenol contents, flavor index, chroma, and hue significantly increased with berry maturity. Chitosan delayed the decrease in chlorophyll, phenol contents, antioxidant capacity, berry maturity, and color change. The results show that in the berries coated with 1% chitosan during storage, changes in color were less in comparison with the control condition (no chitosan). The highest decrease of hue angle and color index (17.92°) was observed in the control. The highest total antioxidant (78.42%) was observed after 30 days of storage in MG berries treated with a 0.5% chitosan coating.

Monitoring Meat Freshness with Intelligent Colorimetric Labels Containing Red Cabbage Anthocyanins Copigmented with Gelatin and Gallic Acid

Polyvinyl alcohol (PVA)-based pH-responsive color indicators were developed using red cabbage anthocyanin (Anth) copigmented with gelatin and gallic acid (GA). The indicator prepared with gelatin and GA (GA/gelatin/Anth/PVA) was highly resistant to light exposure. GA/gelatin/Anth/PVA exhibited distinct color changes in pH 2–11 buffer solutions and stable color indication in acidic and neutral solid systems (pH 2 and 7) at 97% relative humidity. GA/gelatin/Anth/PVA exhibited the highest sensitivity to dimethylamine, followed by ammonia and trimethylamine. The addition of gelatin and GA facilitated hydrogen bonding, which enhanced thermal stability and water solubility without compromising tensile properties. A color change from purple to blue signaled spoilage when total volatile basic nitrogen values for beef and squid reached 21.0 and 37.8 mg/100 g, respectively. The GA/gelatin/Anth/PVA indicator shows potential for indicating the freshness of raw beef.

Cu2+-mediated telomeric dimeric G-quadruplex DNAzyme for highly sensitive colorimetric detection of deferasirox.

Deferasirox (DEF) is an important iron chelator for treatment of iron overload-related diseases. Monitoring DEF concentration in human serum will provide some valuable information for clinical diagnosis and therapy of such diseases. In this study, we developed a peroxidase-mimicking colorimetric sensor for the detection of DEF by simple assembly of a telomeric dimeric G-quadruplex DNAzyme with Cu2+. The DNAzyme-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 can generate a quantitative colorimetric signal, and the color change can be discerned by the naked eye. Compared with the reaction rate of the monomeric G-quadruplex-Cu2+ DNAzyme, the reaction rate of the dimeric G-quadruplex-Cu2+ (G2-Cu2+) DNAzyme is significantly accelerated, and the reaction rate gradually increases and then reaches a plateau with increasing number of TTA spacers. Herein, the G2-Cu2+ DNAzyme is chosen for the highly sensitive detection of DEF based on the DEF-Cu2+ complex-induced inhibition of its peroxidase-mimicking activities. The limit of detection (LOD) of DEF is achieved as low as 0.03μM, and the linear range is from 0.05 to 1.2μM. The proposed strategy exhibits excellent selectivity in the presence of potential interferents, such as metal ions and small molecules. Importantly, the G2-Cu2+ DNAzyme is further expanded to detect DEF in dispersible tablets and human serum samples. Overall, this G2-Cu2+ DNAzyme provides a simple, low-cost, and rapid platform for DEF detection. This novel strategy is the first example of DEF analysis by utilizing signal amplification technology based on the G-quadruplex DNAzyme and holds great potential for DEF quality control and therapeutic drug monitoring.

Discovery of EDA‐Complex Photochemical Reactions Using Computational Vision

Herein, we report a multidimensional screening strategy to discover Electron Donor‐Acceptor (EDA) complex photochemical reactions using only simple photographic devices, such as webcams or cellphones, combined with TLC analysis. An algorithm was designed to automatically identify EDA‐complex reactive mixtures in solution by processing digital images from a 96‐well microplate, alongside TLC results. The code detects the absorption region of the mixture in the visible spectrum and quantifies color changes through grayscale values. Integrated automatic TLC analysis further classifies mixtures as colorimetric reactions, non‐reactive, or potentially reactive EDA complexes. Leveraging this screening platform, we have developed a new EDA‐mediated approach to synthesize iminophosphoranes in yields up to 90%.

Combined Transcriptome and Metabolome Analyses Provide New Insights into the Changes in the Flesh Color of Anthocyanins in Strawberry (Fragaria × ananassa (Weston) Duchesne ex Rozier)

Background: Strawberries are bright in color, sweet and sour in taste, and rich in nutrients and flavonoid compounds such as anthocyanins and proanthocyanidins. The synthesis and accumulation of anthocyanins are the decisive factors that make strawberries appear bright red. From the perspective of plant breeding, a change in flesh color is an important goal. Methods: In this study, two strawberry plants with different flesh colors were selected, and transcriptome and metabolome analyses were performed during the color change period (S1) and ripening period (S2). Results: RNA-seq revealed a total of 13,341 differentially expressed genes (DEGs) between and within materials, which were clustered into 5 clusters. A total of 695 metabolites were detected via metabolome analysis, and 243 differentially regulated metabolites (DRMs) were identified. The anthocyanin biosynthesis, starch and sucrose metabolism and glycolysis/gluconeogenesis pathways were determined to be important regulatory pathways for changes in strawberry flesh color through a joint analysis of RNA-seq data and the metabolome. The leucoanthocyanidin reductase (LAR) and chalcone synthase (CHS) gene is a key gene related to anthocyanins, cinnamic acid, and phenylalanine. In addition, through joint RNA-seq and metabolome analyses combined with weighted gene co-expression network analysis (WGCNA), we identified 9 candidate genes related to strawberry flesh color. Conclusions: Our research findings have laid the groundwork for a more comprehensive understanding of the molecular mechanisms governing the color transformation in strawberry flesh. Additionally, we have identified novel genetic resources that can be instrumental in advancing research related to strawberry color change.

A Novel Approach for Colorimetric Detection of Glyphosate in Food Based on a Split Aptamer Nanostructure and DNAzyme Activity.

Glyphosate has become the most widely used herbicide worldwide in recent years. There are many concerns about toxicity and mutagenicity from long-term use of glyphosate in humans and animals. Therefore, the methods that can help in easy and quick detection of this chemical compound in food and water are critical. In this work, a biosensor was fabricated by combining the enzymatic properties of a specific DNA G-quadruplex and selectivity of a split aptamer to detect glyphosate in foods and water in a quick and simple colorimetric manner. The color change in this method is based on the oxidation of TMB by the G-quadruplex enzyme and the function of aptamer to trap glyphosate, which is visible to the naked eye in the presence and absence of the herbicide. The biosensor showed its high performance in various real samples of water and foods and provided a detection limit of 1.37 nM (R² = 0.9899) with a linear response range of 100 to 400 nM of glyphosate. This biosensor can provide an innovative, cheap and fast approach for the detection and monitoring of glyphosate in various foods and water.

A handheld fluorescent platform integrated with a Sm(III)-CdTe quantum dot-based ratiometric nanoprobe for point-of-use determination of phosphate.

Phosphate (Pi) is crucial for various physiological processes and aquatic environments, which emphasizes the need for a simple, on-site sensor to promptly detect Pi for human health and environmental conservation. In this study, we propose a dual-emission ratiometric fluorescence sensor for highly sensitive and visual Pi detection. The sensor employs samarium ions (Sm3+) as a core component, with cadmium telluride quantum dots (CdTe QDs) and ofloxacin (OFL) serving as signal carriers. The CdTe-Sm(III)-OFL nanoprobe emits a purple fluorescence resulting from the red fluorescence of CdTe QDs and the blue-green fluorescence of OFL. The fluorescence of OFL is quenched by Sm3+ through fluorescence resonance energy transfer (FRET). Upon Pi interaction, the FRET process is disrupted due to the competitive Pi-Sm3+ binding, which leads to the fluorescence recovery of OFL while the red fluorescence of CdTe remains steady. This enables the construction of a ratiometric fluorescent sensor for Pi detection, manifesting as a color change from purple to blue. The sensor demonstrated a linear response for Pi detection within the range of 0.1-75 μM, with a low detection limit of 17.0 nM. By utilizing the distinct fluorescence responses of various physiological phosphates and employing chemometrics, this innovative dual-emission sensor accurately distinguishes among different physiological phosphates. Furthermore, a portable lab-on-paper device based on CdTe-Sm(III)-OFL, coupled with a smartphone-integrated mini-device, is developed for swift Pi detection using an ordinary smartphone. Analytical performance validated on environmental and biological samples demonstrates the sensor's excellent robustness and adaptability. This study introduces a pioneering approach to fabricate ratiometric fluorescence sensors and customize portable, cost-effective mini-devices for precise target detection, thus opening avenues for advanced sensing strategies in various applications.

Synthesis of Bis(2,6-Diadamantyl Aryloxide) Ln(II) Complexes of Samarium, Europium, and Ytterbium and Their Ln(III) Precursors.

The syntheses of Ln(II) bis(aryloxide) complexes of Sm, Eu, and Yb have been examined with the bulky aryloxide ligand (OC6H2-2,6-Ad2-4-tBu)1- [(OAr*)1-; Ad = 1-adamantyl]. These LnII(OAr*)2(THF)2 complexes were pursued for comparison with the tris(aryloxide) Ln(II) complexes [LnII(OAr*)3]1- (Ln = La, Ce, Nd, Gd, Dy, and Lu), which have 4fn5d1 electron configurations, and with 4f14 [YbII(OAr*)3]1-. Although the Ln(II) chemistry of Sm, Eu, and Yb is often similar since they all adopt 4fn+1 electron configurations, their chemistry is surprisingly diverse with (OAr*)1-. Reactions of LnIII2(THF)2 with KOAr* in THF form the bis(aryloxide) Ln(II) complexes LnII(OAr*)2(THF)2, 1-Ln, that were characterized by X-ray diffraction for 1-Sm and 1-Yb, but crystals of 1-Eu have been elusive. Reduction of the tris(aryloxide) Sm(III) complex, SmIII(OAr*)3, 2-Sm, prepared from SmIII(NR2)3 (R = SiMe3) and HOAr* in refluxing toluene, generated the bis(aryloxide) complex, 1-Sm, and KOAr* rather than a Sm analogue of [YbII(OAr*)3]1-. Reduction of EuIII(OAr*)3, 2-Eu, prepared from EuCl3 with KOAr* in THF, caused an immediate dark blue-purple to bright red-orange color change, but crystals of the product were elusive. Neither reduction of TmIII(OAr*)3, 2-Tm, nor the reaction of TmIII2(DME)3 with KOAr* provided crystalline Tm(II) complexes.

One-Step Polyester Dyeing and Antibacterial Treatment Using Innovative Thiazole Azo Dyes in Supercritical Carbon Dioxide

AbstractA novel approach in the field, a range of new azothiazole dyes substituted with different hydrophobic groups, was utilized as colorants for dyeing polyester fabrics in a supercritical carbon dioxide environment. The innovative dyes were analyzed using standard spectroscopy techniques and elemental analysis. The dyeing process was assessed for its dyeing ability, color strength, and color fastness for twelve different dyes. The polyester fabrics dyed with these dyes appeared in various shades, between red and magenta. Using the AATCC method, the antibacterial assessment revealed that some dyes exhibited significant antibacterial properties against both positive and negative bacteria. All 12 dyes showed outstanding resistance to washing, rubbing, and light, with staining and color change ratings of 4–5. The results indicate that the synthesized dyes could be employed for large-scale, energy-efficient, and environmentally friendly polyester fabric dyeing utilizing supercritical carbon dioxide. Graphical abstract

Detection of Two Common ACCase Mutations Associated with High Levels of Fenoxaprop-P-Ethyl Resistance in Shortawn Foxtail (Alopecurus aequalis) Using Loop-Mediated Isothermal Amplification

Abstract The resistance to fenoxaprop-P-ethyl, a herbicide that inhibits acetyl-CoA carboxylase (ACCase), has emerged in shortawn foxtail (Alopecurus aequalis Sobol.) since the 1990s, presenting a considerable challenge to wheat (Triticum aestivum L.) production in China. One of the primary mechanisms responsible for this high-level resistance is the presence of mutations at codons 1781, 2041, and 2078 in the ACCase gene. However, the conventional methods used to detect these mutations, such as polymerase chain reaction (PCR) and gene sequencing, are time-consuming and labor-intensive. To address this issue and enable the prompt and effective detection of these common ACCase mutations in A. aequalis, a loop-mediated isothermal amplification (LAMP) strategy was developed. The LAMP assay specifically targets the Ile-1781-Leu and Asp-2078-Gly mutations within the ACCase gene of A. aequalis. Through the optimization of primers, systems, and conditions, the LAMP assay enables rapid differentiation between wild-type individuals and mutants of A. aequalis carrying either of these two mutations. By including SYBR Green I dye in the final reaction mixtures, the target mutation can be visually detected through a noticeable color change that can be observed with the naked eye. It is noteworthy that the sensitivity of the LAMP assay was approximately 104-fold greater than that of conventional PCR methods. Additionally, a derived cleaved amplified polymorphic sequence (dCAPS) assay was established for each mutation to distinguish between homozygous and heterozygous mutants. Overall, the developed LAMP assay could efficiently detect the Ile-1781-Leu and Asp-2078-Gly mutations in the ACCase gene of A. aequalis, offering significant advantages for the monitoring and management of fenoxaprop-P-ethyl resistance.

Colorimetric aptasensing of microcystin-LR using DNA-conjugated polydiacetylene.

Polydiacetylene (PDA) holds promise as a versatile material for biosensing applications due to its unique optical properties and self-assembly capabilities. In this study, we developed a colorimetric detection biosensor system utilizing PDA and aptamer for the detection of microcystin-LR (MC-LR), a potent hepatotoxin found in cyanobacteria-contaminated environments. The biosensor was constructed by immobilizing MC-LR-specific aptamer on magnetic beads, where the aptamer was hybridized with a urease-labelled complementary DNA (cDNA-urease). Upon binding MC-LR, the aptamer undergoes a conformational change to release cDNA-urease. The released cDNA-urease is subsequently captured by PDA bearing a single-stranded DNA (ssDNA). The enzymatic reaction triggers a distinctive color transition of PDA from blue to red. The results demonstrate exceptional sensitivity, with a linear detection range of 5-100ng/mL and a limit of detection as low as 1ng/mL. The practicability of the colorimetric method was demonstrated by detecting different levels of MC-LR in spiked water samples. The recoveries ranged from 77.3 to 102% and the color change, visible to the naked eye, underscores the practical utility for on-site applications. Selectivity for MC-LR over other microcystin variants (MC-RR and MC-YR) was confirmed. The colorimetric detection platform capitalizes on the properties of PDA and nucleic acid, offering a robust method for detecting small molecules with potential applications in environmental monitoring and public health.

The true incidence and risk factors of hemolysis in patients with Impella CP placement

Abstract Background The Impella, a micro axial-flow pump, is effective in treating cardiogenic shock. However, compared to other mechanical circulatory support devices, its high pump speeds can result in complications like hemolysis and subsequent renal failure. Previous reports have defined hemolysis based on the elevation of LDH levels or the presence of hemoglobinuria, but this approach may underestimate its occurrence. Hemolysis is commonly assessed using the hemolysis index, which is calculated through biochemical auto-analysis based on the absorbance measure of the red color change. However, since the hemolysis index is not highly sensitive, it is recommended to measure plasma free hemoglobin (PFHb) for accurate diagnosis of hemolysis. Purpose The aim of this single-center retrospective cohort study is to investigate the frequency of hemolysis in Impella CP using PFHb measurements and its association with the Impella support level. Methods Between December 2019 and June 2023, we included 116 patients who had the Impella CP device implanted at our hospital. For these patients, PFHb was measured twice daily during the Impella placement period (up to 13 days). A PFHb value of 0.06 or higher was considered indicative of hemolysis, at which point a haptoglobin preparation of 4000 units was administered. Separately, the hemolysis index was defined as hemolysis at a level of 41 mg/dL. We investigated the relationship between daily Impella settings, PFHb, and hemolysis index for each patient. Results The average age was 70 ± 13 years. 59% underwent ECPELLA, and 15% were introduced to renal replacement therapy (RTT) due to severe renal failure. During Impella placement, hemolysis was diagnosed in 33% of patients based on PFHb, while only 18% were diagnosed with hemolysis according to the hemolysis index. Upon separate analysis for Impella and ECPELLA during the initial three days, the support levels of Impella were significantly higher in the Impella group on Days 1 and 2, with no significant difference noted on Day 3. Moreover, PFHb levels were significantly higher in the Impella group on Day 1, while on Day 3, the levels were higher in the ECPELLA group. Patients on RRT had significantly higher max PFHb levels than those not on RRT (0.19 vs. 0.06 g/dL; p < 0.001). Next, we investigated the relationship between PFHb levels and Impella support levels in patients with either Impella or ECPELLA over 711 data points. Dividing Impella support into low (P1-3), middle (P4-6), and high (P7-9) categories, PFHb values were significantly higher at high P levels compared to low and middle P levels. Similar results were observed for both Impella and ECPELLA. Conclusion There is a possibility of underestimating hemolysis associated with Impella if PFHb measurement is not performed. Hemolysis showed a significant correlation with higher P levels (P7 and above) for both Impella and ECPELLA.

Iodine‐DMSO Catalyzed β‐carboline Derivative Chemoselective Fluorescent Molecule for Detection of Fluoride and Cyanide Anion

AbstractIn this work, a simple and highly sensitive fluorescent receptor 1‐(p‐tolyl)‐4,9‐dihydro‐3H‐pyrido[3,4‐b]indole (receptor 1), has been synthesized for the detection of fluoride and cyanide ions. The receptor 1 was very selective towards fluoride and cyanide anions over other anions such as Cl−, Br−, I−, HSO4−, NO3−, HPO42−, and OAc−. Synthesized receptor 1 shows low detection limits 3.29 × 10−8 M for fluoride ion, which was determined by UV–vis absorption. While LOD for cyanide was observed to be 61.5 nM. Importantly, while, receptor 1 produced binding constant for fluoride and cyanide in DMSO‐H2O (1:9, v/v) was found to be 5 × 10−4M−1 and 3.28 × 10−5 M−1, respectively. The variation in the optical and fluorescence spectroscopy confirms the formation of N─H⋯F− and N─H⋯CN− hydrogen bond of the pyrrolic proton The sensing mechanism displayed by receptor 1 for fluoride and cyanide ions was confirmed by 1H‐NMR and FT‐IR spectroscopic along with density functional theory (DFT) calculations. Solution with the fluoride and cyanide ions in DMSO‐H2O (1:9, v/v) shows a color change from light yellow to blue under UV light and yellow to colorless in visible light.