ObjectivesThis study aimed to evaluate the effect of whitening toothpastes on the surface roughness and colour change of CAD-CAM materials.Materials and methodsA total of 96 samples (2 × 10 × 12 mm3) were prepared from Cerasmart (CS) and Celtra Duo blocks. Celtra Duo samples were divided into two groups. One group was fired with glaze paste (CDG) and the other was not treated (CD). All groups were then divided into 4 subgroups (n = 8). The groups were brushed with conventional (Colgate™ Max Fresh), silica (Opalescence™), charcoal (Curaprox™ Black in White) and blue covarine (Signal™ White Now) toothpastes for 30,000 brushing cycles. The initial and final surface roughness values were measured with contact profilometer and a dental spectrophotometer used for obtaining colour values. One sample from each brushed group was analyzed using a scanning electron microscope. Data was analyzed with Kruskal-Wallis and Mann-Whitney tests (p = 0.05).ResultsThe surface roughness of CS samples brushed with Opalescence™ and Curaprox™ was significantly higher than CD and CDG. Surface roughness change values of CS samples brushed with Curaprox™ were significantly higher than the CD and CDG. Curaprox™ brushed samples showed significant difference in colour change values for all materials.ConclusionsBrushing increases the surface roughness of CAD-CAM ceramic materials. The roughness of resin-based materials is higher than zirconia-reinforced lithium silicates. Silica-contained toothpastes may cause discoloration of nanoceramic and zirconia-reinforced glass ceramic restorations.Clinical relevanceIt should be clinically considered that whitening toothpastes may cause roughness in ceramic materials and change the desired color.