3D Color Research Articles

Abstract 13922: Association Between Quadrileaflet Tricuspid Valve and Location of Regurgitation Jet Origin in Patients With Functional Severe Tricuspid Regurgitation

Introduction: It has been known that the posterior leaflet of the tricuspid valve (PTL) has several indentations or clefts in its free edge, giving the tricuspid valve (TV) equal to or more than four leaflets appearance. However, little is known about whether the indentations or clefts can be a location of tricuspid regurgitation (TR) jet origin in severe TR. Hypothesis: This study aimed to investigate the association between quadrileaflet TV and location of TR jet origin in patients with functional severe TR. Methods: We retrospectively studied 85 functional severe TR patients who underwent 3D transesophageal echocardiography which was performed using the EPIQ7 ultrasound system with a X8-2t probe (Philips Medical Systems). The morphology of the TV leaflets was ascertained using 3D volume-rendered images. Clefts extending over 50% of the depth of the leaflets were determined as a landmark between 2 adjacent leaflets. The location of TR jet origin and vena contracta area (VCA) were ascertained using real-time 3D color Doppler images. All images were analyzed by using QLAB 3D quantification software (Philips Medical Systems). Results: In 85 functional severe TR patients, a total of 3 (4%) patients with bileaflet, 60 (71%) patients with trileaflet and 22 (26%) patients with quadrileaflet were identified. All quadrileaflet TV had dichotomous PTLs (PTL 1 and PTL 2) and there was no patient who had equal to or more than five leaflets. In 22 patients with quadrileaflet, 10 (45%) patients showed TR jet origin located between PTL 1 and PTL 2 (Figure A). Also, quadrileaflet TV patients with TR jet origin located between PTL 1 and PTL 2 had significantly larger VCA than those without (185 vs. 62 mm2, P<0.001, Figure B). Conclusions: In patients with functional severe TR, one fourth patients showed quadrileaflet TV morphology. Furthermore, TR jet origin located between PTL 1 and PTL 2 can be seen only in a condition of TR beyond severe.

Colored 3D Path Extraction Based on Depth-RGB Sensor for Welding Robot Trajectory Generation

The necessity for intelligent welding robots that meet the demand in real industrial production, according to the objectives of Industry 4.0, has been supported owing to the rapid development of computer vision and the use of new technologies. To improve the efficiency in weld location for industrial robots, this work focuses on trajectory extraction based on color features identification on three-dimensional surfaces acquired with a depth-RGB sensor. The system is planned to be used with a low-cost Intel RealSense D435 sensor for the reconstruction of 3D models based on stereo vision and the built-in color sensor to quickly identify the objective trajectory, since the parts to be welded are previously marked with different colors, indicating the locations of the welding trajectories to be followed. This work focuses on 3D color segmentation with which the points of the target trajectory are segmented by color thresholds in HSV color space and a spline cubic interpolation algorithm is implemented to obtain a smooth trajectory. Experimental results have shown that the RMSE error for V-type butt joint path extraction was under 1.1 mm and below 0.6 mm for a straight butt joint; in addition, the system seems to be suitable for welding beads of various shapes.

OVERTON-RESONANT SINGING BASIS OF COMPOSITOR LANGUAGE (OVERTONE-RESONANT BASIS OF COMPOSITOR LANGUAGE)

The article substantiates the embodiment of the traditional overtone intonational song beginning in the composer’s work. The application of the main acoustic features of an authentic timbre is described, such as compression, the presence of «specific resonances», reliance on the vocal position and inharmonic overtones. The main composer’s task is to create a piece of music that would imitate or reproduce the resonance characteristic of the folk singing tradition — the overtone. The research is based on the method of sound-acoustic measurement of an audio signal. The work was performed using the Reaper V4,581 audio editor software (Digital Audio Workstation) and a spectrograph built into the Reaper brand iZotop Ozon5 Advansed. This model of the spectrograph allows you to see the spectrogram of the investigated audio material in 3D format and color image, which more accurately reflects the measurements of the vibration frequency of the sound wave, and is also equipped with a broadband FFT analyzer. The scientific novelty of the research lies in the consideration of composer folklorism from the point of view of the timbre acoustic model of authentic singing, and the adaptation of expressive means is investigated not within the framework of the style and manner of the melodic presentation of the material of individual composers, but from the point of view of the timbre content of the folklore voice. The article shows how the influence of ethno-singing on composer’s creativity is revealed through the prism of authentic timbre and the given spectral examples.

Substrate characterization of the right ventricle in repaired tetralogy of Fallot using late enhancement cardiac magnetic resonance.

Three-dimensional (3D) substrate characterization by high-resolution late gadolinium enhancement cardiac magnetic resonance (LE-CMR) is useful for guiding ventricular tachycardia ablation of the left ventricle in ischemic heart disease. The purpose of this study was to validate the substrate characterization and 3D reconstruction of LE-CMR images of the right ventricle (RV) in patients with repaired tetralogy of Fallot (rTOF) and to identify the algorithm that best fits with electroanatomic mapping (EAM). RV LE-CMR images were compared with RV EAM in 10 patients with rTOF. RV LE-CMR images were postprocessed and analyzed to identify fibrotic tissue on 3D color maps. The 3D RV substrate reconstructions were created using an adjustable percentage of the maximum voxel signal intensity (MSI) of the scar region to define the threshold between core, transitional zone (TZ), and healthy tissue. Extensions of the core and TZ areas were compared with the scar (<0.5 mV) and low-voltage (0.5-1.5 mV) areas obtained by RV EAM. Agreement on anatomic isthmi identification was quantified. The best match between core and scar was obtained at 65% MSI cutoff (mean areas 17.4 ± 9.9 cm2 vs 16.9 ± 10.0 cm2, respectively; r = 0.954; P <.001). Agreement on anatomic isthmi identification was best at 60% MSI cutoff, which identified 95% of isthmi and achieved a total fit in 90% of patients. This study demonstrates that characterization of the RV substrate by postprocessing LE-CMR images in rTOF patients is feasible and validates the technique against RV EAM, which could help in planning target ablation.

Phase-only color rainbow holographic near-eye display.

Color rainbow holography can realize color holographic 3D display without speckle noise under white light illumination. However, traditional color rainbow holograms used for high-resolution static color 3D display or near-eye color display are amplitude-type, resulting in low diffraction efficiency due to the presence of conjugate light. In this Letter, a phase-only color rainbow holographic near-eye display is demonstrated. The calculation of a phase-only color rainbow hologram is realized by combining a band-limited diffraction and a bi-directional error diffusion algorithm with high-frequency blazed gratings coded to control longitudinal dispersion. When the wavelength of illumination light is deviated from the designated wavelength of the hologram, only a certain wavefront aberration is caused, but there is no conjugate light. The phase-only color rainbow holographic near-eye display of both a 2D color image and a 3D scene are implemented by optical experiments. It has potential applications in head-mounted 3D augmented reality displays without vergence-accommodation conflict.

Systematic Review of Clinical Applications of CAD/CAM Technology for Craniofacial Implants Placement and Manufacturing of Orbital Prostheses.

This systematic review was aimed at gathering technical and clinical applications of CAD/CAM technology for the preoperative planning of craniofacial implants placement, designing of molds and substructures and fabrication of orbital prostheses. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, an electronic search was executed. Human studies that utilized digital planning systems for the prosthetic rehabilitation of orbital defects were included. A total of 16 studies of 30 clinical cases, which were virtually planned through various digital planning and designing software, were included. The most common preoperative data required for digital planning were CT scans in 15 cases, the 3DSS-STD-II scanning system in 5 cases, an Artec Color 3D scanner in 3 cases and a NextEngine Desktop 3D laser scanner in 2 cases. Meanwhile, the digital designing software were Ease Orbital Implant Planning EOIPlan software in eight cases, Geomagic software in eight cases, Simplant software in four cases and Artec Studio 12 Professional in three cases. Surgical templates were fabricated for 12 cases to place 41 craniofacial implants in the orbital defect area. An image-guided surgical navigation system was utilized for the placement of five orbital implants in two cases. Digital designing and printing systems were reported for the preoperative planning of craniofacial implants placement, designing of molds and substructures and fabrication of orbital prostheses. The studies concluded that the digital planning, designing and fabrication of orbital prostheses reduce the clinical and laboratory times, reduces patient visits and provide a satisfactory outcome; however, technical skills and equipment costs are posing limitations on the use of these digital systems.

In Silico Screening of a Bile Acid Micelle Disruption Peptide for Oral Consumptions from Edible Peptide Database.

Recently, many bioactive peptides have been identified using bioinformatics tools. Previously, our group developed a method to screen dual-functional peptides that have direct intestinal delivery with porous silica gel and bile acid micelle disruption. However, newly designed peptides were not found in any storage protein. Therefore, in this study, in silico screening was performed using a 350,000 edible peptide library consisting of 4- to 7-mer independent peptides. As an initial screening, all edible peptides were applied to the random forest model to select predicted positive peptides. For a second screening, the peptides were assessed for the possibility of intestinal delivery using a 3D color map. From this approach, three novel dual-functional peptides, VYVFDE, WEFIDF, and VEEFYC were identified, and all of them were derived from storage proteins (legumin, myosin, and 11S globulin). In particular, VEEFYCS, in which a serine residue (S) is added to VEEFYC, was assumed to be released by thermolysin from the 11S-globulin derived from Ginkgo biloba by LC-MS/MS analysis. VEEFYCS was found to have suitable direct intestinal delivery and bile acid micelle disruption activity.

Multi-modal compound biometric feature set security and person authentication using cancelable 2D color barcode pattern generation technique

Multi-modal compound biometric feature set security and person authentication using cancelable 2D color barcode pattern generation technique

A Case of Déjà Vu: Surgical Correction of a Uterine Avm and Hysteroscopic Resection of Rpoc Secondary to Invasive Placenta

<h3>Study Objective</h3> To demonstrate a unique case of both a laparoscopic correction of a uterine AVM and hysteroscopic resection of retained products of conception secondary to invasive placenta. <h3>Design</h3> A brief literature review and case report with demonstration of surgical technique. <h3>Setting</h3> Operating Room environment. <h3>Patients or Participants</h3> In this video we explore the case of a 28-year-old patient who in 2019 had a 20-week loss followed by PPH requiring manual removal of retained placenta and D&C. Subsequent to this first pregnancy she a delayed PPH with IR embolization of the left uterine artery for a uterine AVM. In 2020, the patient delivered vaginally, preterm at 27 weeks and again had a PPH requiring manual removal of retained placenta and D&C. After 2 months of continued spotting an ultrasound queried residual RPOC with hypervascularity in the right uterine wall suggestive of a right AVM with CT angiography confirming a uterine AVM. <h3>Interventions</h3> The patient wished for fertility preservation and ultimately underwent laparoscopic clipping of the feeding vessel, the right internal iliac artery followed by hysteroscopic removal of retained products of conception. <h3>Measurements and Main Results</h3> The patient's post-operative course was uncomplicated and final pathology confirmed normal placental tissue consistent with placenta accreta. The post-op sonohysterogram confirmed an empty uterine cavity and the 2D colour doppler US confirmed reduced right uterine wall vascularity. <h3>Conclusion</h3> A laparoscopic approach to uterine AVM management is possible and can be combined with hysteroscopic investigation and management of RPOC.

Prognostic value of three dimensional-vena contracta area in patients with secondary mitral regurgitation

Abstract Background Effective regurgitant orifice area (EROA) is an important quantitative measurement for mitral regurgitation (MR) grading. Yet, the accuracy of this method is limited in patients with secondary mitral regurgitation (SMR). Three-dimensional (3D) color Doppler echocardiography allows for the direct assessment of the vena contracta area (VCA). The prognostic value of 3D-VCA in patients with secondary MR has not been investigated. Purpose The aim of the present study was to assess the association between 3D-VCA and prognosis of patients with SMR. Methods A total of 218 patients (69% men, median age 74 years) with significant SMR were retrospectively analyzed. 3D-VCA was measured offline with dedicated software, from restored 3D color Doppler full volume datasets of the mitral valve (Figure 1). The population was divided according to the American College of Cardiology expert recommendation for the grading of severe MR (VCA ≥50 mm2 and VCA &amp;lt;50 mm2). Patients were followed up for the combined end point of all-cause mortality or heart failure hospitalization. Results Of the total population, 63% had an ischemic etiology, 60% had atrial fibrillation and 25% cardiac resynchronization therapy. Patients with 3D-VCA ≥50 mm2 needed more diuretic therapy, had a larger left ventricle and atrium, and had more post-procedural residual MR. A total of 82% of patients underwent MitraClip device implantation, 17% had mitral valve repair and 1% had mitral valve replacement. During a median follow-up of 28 months, 130 (60%) met the combined end point (101 (46%) patients died and 81 (37%) were hospitalized due to heart failure). When dividing the population according to the cut-off of 3D-VCA, patients with a 3D-VCA≥50 mm2 had a worse prognosis compared with their counterparts (Figure 2). In a multivariable Cox regression analysis, 3D-VCA≥50 mm2 remained independently associated with the composite endpoint of all-cause mortality or heart failure hospitalization (HR=1.454, 95% CI 1.020–2.072, p=0.038). Conclusion In patients with SMR, a 3D-VCA ≥50 mm2 was independently associated with a combined endpoint of death or heart failure hospitalization. Funding Acknowledgement Type of funding sources: None. Figure 1. Method of 3D-VCA measurementFigure 2. Kaplan-Meier survival curve

Effect of interventional edge-to-edge repair in tricuspid regurgitation on ring dimensions

Abstract Background The concept of percutaneous tricuspid valve edge-to-edge repair (pTVR) is based on the connection of leaflets in the area of insufficiency using a coaptation device. By closing the coaptation device a considerable tractive force is applied on the leaflets, which might have an effect on the valve ring. Aim of the study was to examine the impact of device implantation on tricuspid ring dimensions. Methods During pTVR 3D zoom loops of tricuspid valve were acquired before and after clip placement using transoesophageal echocardiography. Measurements of TV ring dimensions included the following parameters: ring area (TV area), maximal diameter, minimal diameter, eccentricity index (Figure 1). Tenting area was derived from a four-chamber view of the valve. In addition, regurgitation severity was graded from 1+ to 5+ by measuring vena contracta area (VCA3D) in 3D full volume colour Doppler loop using multiplanar reconstruction. Right atrial (RA) and ventricular volumes (RVVd3D, RVVs3D) and function (RVEF3D) were assessed in a 3D full volume loop. Results The study population comprised 97 patients (age 78±6 years, 47 male), who underwent pTVR at our hospital. As expected cavity dimension correlated with TV area size (for RVVd3D r=0.51, p&amp;lt;0.001 and for RA volume r=0.71, p&amp;lt;0.001). The mean TV ring area was significantly reduced (ring area 8.53±2.23 cm2/m2BSA vs. 7.55±2.18 cm2/m2BSA, p&amp;lt;0.001) and the ring shape became more oval (Eccentricity index 1.2±0.15 vs. 1.29±0.17, p&amp;lt;0.001) after pTVR. The reduction of ring area (12±7%, range 0.7–28%) showed an only modest correlation to the number of implanted coaptation devices (r=0.30, p&amp;lt;0.001) and percentage reduction of VCA3D (r=0.36, p&amp;lt;0.001). In the patient group with a ring area change ≥12% a reduction to TR grade ≤2+ by pTVR was achieved in 83% of cases, whereas only 62% of patients reached moderate TR when area change was below 12%. Conclusion pTVR using coaptation devices reduces the ring area. This effect is related to the number of devices implanted. Funding Acknowledgement Type of funding sources: None.

Impact of endocardial leads on the effectiveness of interventional edge-to-edge repair of tricuspid regurgitation

Abstract Background A considerable proportion of patients who are considered for percutaneous tricuspid valve edge-to-edge repair (pTVR) interventions have endocardial lead induced tricuspid regurgitation (TR). The aim of this study was to examine the impact of lead-leaflet interaction on the effectiveness of pTVR. Methods For each patient, the lead position within the tricuspid valve (central, commissural, or towards one of the three leaflets) and the type of lead-leaflet interaction (leaflet impingement or adhesion) were identified during thorough 2D/3D transthoracic and transesophageal echocardiography examinations. Before and after pTVR, echocardiographic data, including 3D full-volume datasets, were obtained and quantified. TR severity was graded from 1+ to 5+, based upon the effective regurgitant orifice area by the PISA method (EROAPISA) and the vena contracta area (VCA3D) as measured by multiplanar reconstruction from a 3D color Doppler loop. Maximal diastolic tricuspid annulus area from a 3D zoom image, tricuspid tenting area, and right atrial volume were quantified. Right ventricular assessments included ejection fraction (RVEF3D) and diastolic (RVVd3D) and systolic (RVVs3D) volumes. Results Out of 99 patients who underwent pTVR at our hospital, 38 patients had implanted cardiac devices of the following types: pacemakers (n=25, 66%), cardiac defibrillators (n=7, 18%), and biventricular pacemakers (n=6, 16%). In 24 (63%) of these device patients, TR grade was ≤2+ after pTVR. In 14 of the device patients, TR grade remained severe after intervention (Grade 3+ in 15%, grade 4+ in 11%, and Grade 5+ in 11% of the device group). In comparison, in 78% of patients without endocardial leads, only moderate TR was achieved after pTVR. Figure 1 shows the distribution of lead positions within the tricuspid valve. A relevant lead-leaflet interference (rLLI) for pTVR interventions was defined as impingement or adhesion in the target area of the coaptation device (anteroseptal or posteroseptal). Binary logistic regression analysis showed an increase risk (Odds ratio 11, R2 0.34, 95% CI 0.019–0.44, p=0.003) for a suboptimal pTVR result (TR grade &amp;gt;2+ after intervention) in patients with rLLI. Although patients with rLLI (n=17) had significantly higher VCA3D values as compared to patients without rLLI (n=21), it was not predictive for pTVR results. Echocardiographic parameters of right ventricular and tricuspid valve dimensions showed only a trend to higher values in the group with rLLI (Table 1). Conclusion Endocardial lead induced TR negatively impacts the effectiveness of pTVR irrespective of the initial degree of TR. Funding Acknowledgement Type of funding sources: None.

Echocardiographic predictors of the effectiveness of interventional edge-to-edge repair in tricuspid valve regurgitation

Abstract Background Percutaneous tricuspid valve edge-to-edge repair (pTVR) is a promising interventional technique for patients with tricuspid regurgitation (TR), but guidance regarding patient selection and echocardiographic screening is lacking. The aim of this study was to identify echocardiographic parameters which may predict pTVR success. Methods Before and after pTVR, echocardiographic data, including 3D full-volume datasets, were obtained and quantified. Right ventricular assessments included ejection fraction (RVEF3D) and diastolic (RVVd3D) and systolic (RVVs3D) volumes. Also evaluated were: right atrial (RA) volume, effective regurgitant orifice area by PISA method (EROAPISA), vena contracta area (VCA3D) by multiplanar reconstruction from a 3D colour Doppler loop (Figure 1a), maximal diastolic tricuspid annulus area from a 3D zoom image (Figure 1b), and tricuspid tenting area. TR severity was graded according to EROAPISA and VCA3D as grade 1+ to 5+. Results Patients (n=99, age 79±6 years, 48 male) with at least moderate to severe TR undergoing pTVR were consecutively included. The patients were divided into groups according to their post-pTVR TR grade. Group 1 had TR grade ≤2+, and group 2 had TR grade ≥3+.Echocardiographic parameters before pTVR for both groups are presented in Table 1. As expected, patients with TVR ≥3+ after pTVR had significantly worse pre-intervention echocardiographic measurements of TR severity, valve dimensions, and chamber volumes. ROC curves for the prediction of TR ≤2+ (mild to moderate) after pTVR (defined as VCA3D &amp;lt;0.75 cm2 and EROAPISA &amp;lt;0.4 cm2) were drawn for different echocardiographic features (Figure 2). VCA3D by 3D colour Doppler yielded the highest area under the ROC curve followed by TV anatomy measurements (Annulus area3D, Tenting area) and right atrial volume. Conclusion A thorough evaluation of TR and valve dimensions by 3D echocardiography, particularly the evaluation of VCA3D by 3D colour Doppler, aids in the prediction of the probability of pTVR success. Funding Acknowledgement Type of funding sources: None.

Multimodal and Multiview Wound Monitoring with Mobile Devices

Along with geometric and color indicators, thermography is another valuable source of information for wound monitoring. The interaction of geometry with thermography can provide predictive indicators of wound evolution; however, existing processes are focused on the use of high-cost devices with a static configuration, which restricts the scanning of large surfaces. In this study, we propose the use of commercial devices, such as mobile devices and portable thermography, to integrate information from different wavelengths onto the surface of a 3D model. A handheld acquisition is proposed in which color images are used to create a 3D model by using Structure from Motion (SfM), and thermography is incorporated into the 3D surface through a pose estimation refinement based on optimizing the temperature correlation between multiple views. Thermal and color 3D models were successfully created for six patients with multiple views from a low-cost commercial device. The results show the successful application of the proposed methodology where thermal mapping on 3D models is not limited in the scanning area and can provide consistent information between multiple thermal camera views. Further work will focus on studying the quantitative metrics obtained by the multi-view 3D models created with the proposed methodology.

Color 3D printing of pulped yam utilizing a natural pH sensitive pigment

A novel color control strategy for 3D printing was proposed based on a single channel nozzle with a single raw material. The basic principle was to regulate the pH of the extruded line through electrolysis which would result in the color change of pH-sensitive pigments mixed in the printed materials. Using pulped yam as a feedstock and a pH sensitive pigment extracted from purple sweet potato (PPP), the process parameters were optimized using a moisture content of 35% and a PPP content of 5 mg/100 g. Printing tests showed that the color of the extruded line could be conveniently controlled by adjusting the applied electrode potential from − 45 to 45 V with a hue range of 0.192–0.916, which exhibited a gradually changing color scheme from mauve to yellow. The proposed strategy showed promising potential in color 3D printing with the advantages of low cost, convenient control and simple composition. • A novel color 3D printing method was developed. • Printed color can be controlled by adjusting electrode potential. • A color scheme ranging from mauve to yellow can be achieved.

Factoring 2048-bit RSA Integers in 177 Days with 13 436 Qubits and a Multimode Memory.

We analyze the performance of a quantum computer architecture combining a small processor and a storage unit. By focusing on integer factorization, we show a reduction by several orders of magnitude of the number of processing qubits compared with a standard architecture using a planar grid of qubits with nearest-neighbor connectivity. This is achieved by taking advantage of a temporally and spatially multiplexed memory to store the qubit states between processing steps. Concretely, for a characteristic physical gate error rate of 10^{-3}, a processor cycle time of 1 microsecond, factoring a 2 048-bit RSA integer is shown to be possible in 177days with 3D gauge color codes assuming a threshold of 0.75% with a processor made with 13 436 physical qubits and a memory that can store 28million spatial modes and 45 temporal modes with 2hours' storage time. By inserting additional error-correction steps, storage times of 1second are shown to be sufficient at the cost of increasing the run-time by about 23%. Shorter run-times (and storage times) are achievable by increasing the number of qubits in the processing unit. We suggest realizing such an architecture using a microwave interface between a processor made with superconducting qubits and a multiplexed memory using the principle of photon echo in solids doped with rare-earth ions.

Value of 2D Ultrasound and Color Doppler Ultrasound in Diagnosis of Benign and Malignant Thyroid Nodules

Value of 2D Ultrasound and Color Doppler Ultrasound in Diagnosis of Benign and Malignant Thyroid Nodules

Identification of pollen taxa by different microscopy techniques.

Melissopalynology is an important analytical method to identify botanical origin of honey. Pollen grain recognition is commonly performed by visual inspection by a trained person. An alternative method for visual inspection is automated pollen analysis based on the image analysis technique. Image analysis transfers visual information to mathematical descriptions. In this work, the suitability of three microscopic techniques for automatic analysis of pollen grains was studied. 2D and 3D morphological characteristics, textural and colour features, and extended depth of focus characteristics were used for the pollen discrimination. In this study, 7 botanical taxa and a total of 2482 pollen grains were evaluated. The highest correct classification rate of 93.05% was achieved using the phase contrast microscopy, followed by the dark field microscopy reaching 91.02%, and finally by the light field microscopy reaching 88.88%. The most significant discriminant characteristics were morphological (2D and 3D) and colour characteristics. Our results confirm the potential of using automatic pollen analysis to discriminate pollen taxa in honey. This work provides the basis for further research where the taxa dataset will be increased, and new descriptors will be studied.

Quantitative analysis of metastatic breast cancer in mice using deep learning on cryo-image data

Cryo-imaging sections and images a whole mouse and provides ~ 120-GBytes of microscopic 3D color anatomy and fluorescence images, making fully manual analysis of metastases an onerous task. A convolutional neural network (CNN)-based metastases segmentation algorithm included three steps: candidate segmentation, candidate classification, and semi-automatic correction of the classification result. The candidate segmentation generated > 5000 candidates in each of the breast cancer-bearing mice. Random forest classifier with multi-scale CNN features and hand-crafted intensity and morphology features achieved 0.8645 ± 0.0858, 0.9738 ± 0.0074, and 0.9709 ± 0.0182 sensitivity, specificity, and area under the curve (AUC) of the receiver operating characteristic (ROC), with fourfold cross validation. Classification results guided manual correction by an expert with our in-house MATLAB software. Finally, 225, 148, 165, and 344 metastases were identified in the four cancer mice. With CNN-based segmentation, the human intervention time was reduced from > 12 to ~ 2 h. We demonstrated that 4T1 breast cancer metastases spread to the lung, liver, bone, and brain. Assessing the size and distribution of metastases proves the usefulness and robustness of cryo-imaging and our software for evaluating new cancer imaging and therapeutics technologies. Application of the method with only minor modification to a pancreatic metastatic cancer model demonstrated generalizability to other tumor models.

Utilizing Intraoperative Navigated 3D Color Doppler Ultrasound in Glioma Surgery.

BackgroundIn glioma surgery, the patient’s outcome is dramatically influenced by the extent of resection and residual tumor volume. To facilitate safe resection, neuronavigational systems are routinely used. However, due to brain shift, accuracy decreases with the course of the surgery. Intraoperative ultrasound has proved to provide excellent live imaging, which may be integrated into the navigational procedure. Here we describe the visualization of vascular landmarks and their shift during tumor resection using intraoperative navigated 3D color Doppler ultrasound (3D iUS color Doppler).MethodsSix patients suffering from glial tumors located in the temporal lobe were included in this study. Intraoperative computed tomography was used for registration. Datasets of 3D iUS color Doppler were generated before dural opening and after tumor resection, and the vascular tree was segmented manually. In each dataset, one to four landmarks were identified, compared to the preoperative MRI, and the Euclidean distance was calculated.ResultsPre-resectional mean Euclidean distance of the marked points was 4.1 ± 1.3 mm (mean ± SD), ranging from 2.6 to 6.0 mm. Post-resectional mean Euclidean distance was 4.7. ± 1.0 mm, ranging from 2.9 to 6.0 mm.Conclusion3D iUS color Doppler allows estimation of brain shift intraoperatively, thus increasing patient safety. Future implementation of the reconstructed vessel tree into the navigational setup might allow navigational updating with further consecutive increasement of accuracy.