Colony Health Research Articles

Beekeepers' perceptions toward a new omics tool for monitoring bee health in Europe.

Pressures on honey bee health have substantially increased both colony mortality and beekeepers' costs for hive management across Europe. Although technological advances could offer cost-effective solutions to these challenges, there is little research into the incentives and barriers to technological adoption by beekeepers in Europe. Our study is the first to investigate beekeepers' willingness to adopt the Bee Health Card, a molecular diagnostic tool developed within the PoshBee EU project which can rapidly assess bee health by monitoring molecular changes in bees. The Bee Health Card, based on MALDI BeeTyping®, is currently on level six of the Technology Readiness Level scale, meaning that the technology has been demonstrated in relevant environments. Using an on-line survey from seven European countries, we show that beekeepers recognise the potential for the tool to improve colony health, and that targeted economic incentives, such as subsidises, may help reduce cost being a barrier to the adoption and frequent use of the tool. Based on the description of the tool, 43% of beekeepers appear to be moderately confident in the effectiveness of the Bee Health Card. This confidence could increase if the tool was easy to use and not time consuming, and a higher confidence could also contribute to raising the probability of accepting extra costs linked to it. We estimate that, in the worst-case scenario, the cost per single use of the Bee Health Card should be between €47-90 across a range of European countries, depending on the labour and postage costs. However, the monetary benefits in terms of honey production could exceed this. In order to successfully tackle colony health issues, it is recommended using the BHC five times per year, from the end to the beginning of winter. Finally, we discuss the knowledge needs for assessing beekeeper health tools in future research.

Insecticide application prevents honey bees from realizing benefits of native forage in an agricultural landscape.

Health and population status of bees is negatively affected by anthropogenic stressors, many of which co-occur in agricultural settings. While pollinator habitat (often involving plantings of native forbs) holds promise to benefit both managed and wild bees, important issues remain unresolved. These include whether conventional, broad-spectrum insecticide use negates these benefits and how non-native, managed honey bees affect wild bees in these areas. We conducted a three-year replicated study in a Midwestern corn and soybean production region (i.e., Iowa, USA). We assessed acute and delayed effects of commercial-scale spraying of a commonly used, foliar-applied insecticide (λ-cyhalothrin) in soybean on the productivity of honey bee colonies kept within these fields. Colony health metrics showed no immediate significant differences between insecticide treated and untreated crop fields. As expected, health metrics declined in all colonies after soybean ceased flowering. Interestingly, the subset of colonies from untreated fields given access to restored prairies rebounded. However, colonies from insecticide-treated fields showed reduced growth, queen egg-laying, and survival, even when given access to prairies. In addition, we did not observe a detectable impact of honey bee apiaries on wild bee abundance or diversity at these prairies over a three-year period. Our findings underscore the complex interactions between agricultural practices and bee health, highlighting the necessity of integrated pest management and the conservation of native floral resources to support pollinator populations and sustainable beekeeping in agroecosystems.

Investigations on Beekeeping and Breeding of Apis cerana in China

The worldwide value of the honey bee as an agricultural animal is increasingly being recognized. Not only does the honey bee directly produce useful agricultural products, but also large portions of crops are dependent on the pollination activities of honey bees. Apis cerana (A. cerana), the native honey bee of China, is widely distributed in the country. Studying the biological environment and colony management of A. cerana is important for its conservation and breeding. This study investigated the apiculture of A. cerana among a total of 201 beekeepers in the eastern, southern, northwestern and central regions of China and conducted data analysis on the surveyed data. The results showed that the most favorite traits for beekeepers are colony size, colony health and honey production. Compared with Apis mellifera ligustica, A. cerana is more adaptable to low temperature and scattered nectar sources. The results help to optimize the breeding programs of A. cerana and further contribute to substantive breeding accomplishments with honey bees which have enhanced their role in modern agriculture.

Optimum sugar syrup feeding intervals for Apis cerana Fab. during the winter dearth period in the sub-tropical area of Nepal

The study aimed to identify the optimal sugar syrup feeding interval for honeybee colonies during the winter dearth period in subtropical region of Nepal to ensure colony survival and maintenance. The focus was on evaluating sealed brood area, sealed honey area, egg-laying activity, and pollen collection. The experiment was conducted in an apiary located in Lamjung district of Nepal. Sugar syrup (1 water: 1 sugar) was fed to honeybee colonies at intervals of 7, 10, 15, and 20-days, along with a control group. Each treatment was replicated across four replications with five beehives in each replication, and data were collected weekly. Parameters such as the sealed brood area, honey storage, egg-laying rate, and pollen cells were measured by counting eggs, pollen cells, and assessing areas covered by sealed brood and honey. Colonies fed sugar syrup at 15-days intervals exhibited the best performance across all measured parameters, including the largest sealed brood area, highest honey reserves, maximum eggs laid by the queen bee, and the most pollen cells. These results indicate that feeding at 15-days intervals supports optimal colony health and activity during low-temperature winter conditions. Feeding sugar syrup at 15-days intervals is the most effective practice for maintaining colony health, ensuring optimal honey reserves, brood production, and pollen collection during the winter dearth period in subtropical areas of Nepal. This feeding strategy is recommended for improving colony survival, maintenance, and overall performance during challenging winter conditions in subtropical region of Nepal.

Discovery of reproductive tissue-associated bacteria and the modes of microbiota acquisition in male honey bees (drones).

Over the last decade, annual honey bee colony loss has increased, resulting in a critical need to determine what factors contribute to honey bee and colony health. Gut microbes have been shown to play important roles in the health of the nonreproductive female honey bee workers, which make up 90% or more of a honey bee colony. However, we currently know very little about the impact of microbes on the health of male honey bees (drones), who only make up a small portion of the colony population but play a very key role in the success of future colonies by mating with virgin queens. Here, we discovered microbes within the reproductive organs of drones and illustrated that social interactions with worker bees are necessary for the proper development of the gut and reproductive tissue microbial communities in drones. Further studies are needed to determine if microbes play an important role in honey bee reproductive health and fitness.

NEONICOTINOIDS VERSUS BEES: A BRIEF REVIEW ON THE EFFECTS ON THESE POLLINATORS AND THE NEED FOR REEVALUATION OF AGRICULTURAL PRACTICES

Neonicotinoids, a class of widely used insecticides in agriculture, have been associated with significant negative impacts on pollinators, especially bees. This study conducts a systematic literature review to assess the effects of these compounds, with a focus on native and wild species. Exposure to neonicotinoids can cause direct mortality and sublethal effects in bees and other pollinators, impairing foraging behavior and colony health. Among the 42 reviewed articles, the European honeybee (Apis mellifera) was the most investigated species, with 17 mentions. However, the inclusion of native species, such as Bombus terrestris and Melipona scutellaris, is gaining prominence, highlighting the need to protect pollinator diversity to ensure agricultural sustainability and biodiversity. The study concludes that a reassessment of agricultural and regulatory practices is urgently needed to mitigate the effects of neonicotinoids on ecosystems.

Selection of Honey Bee (Apis mellifera) Genotypes for Three Generations of Low and High Population Growth of the Mite Varroa destructor.

Honey bee (Apis mellifera) population declines have been associated with the parasitic mite, Varroa destructor, which is currently primarily controlled by the use of acaricides. An alternative is to breed for resistance to Varroa, which was conducted in this study by bidirectional selection for mite fall to obtain colonies with low (resistant) or high (susceptible) Varroa population growth (LVG and HVG, respectively). Selection for three generations resulted in approx. 90% lower Varroa population growth in LVG than in HVG colonies. In addition, late summer Varroa infestation rates of brood and adults were both significantly lower in LVG colonies (p < 0.01), which was also significantly associated with lower Deformed Wing Virus (DWV) infection levels (p < 0.01). Survival of Varroa-parasitized bees was almost 50% higher for LVG bees compared to HVG bees (p < 0.01). Also, colony winter survivorship was significantly higher for LVG colonies than for HVG colonies (p < 0.05). However, the higher colony populations observed for LVG colonies were not significantly different from those of HVG colonies. Overall, individual and colony health was improved by selecting colonies for LVG, demonstrating its effectiveness as a means of breeding for controlling Varroa populations in honey bee colonies.

Dominance of recombinant DWV genomes with changing viral landscapes as revealed in national US honey bee and varroa mite survey

Honey bees are essential pollinators for global agriculture. The viromes of US commercial apiaries and their ectoparasitic mites are poorly characterized at a strain level and there is a need to integrate genomics into pathogen surveillance. We sequenced RNA viromes from 383 adult bees and 173 mites pooled samples from 11 major US beekeeping hubs in 2021, assembling 45 complete and 1702 partial genomes. Protein sequence similarity networks and recombinant genome identification revealed a new viral landscape. Sinaivirus (n = 312), Iflavirus sacbroodi (n = 280), and Iflavirus aladeformis (DWV, n = 135) genomes were common. Recombinant DWV genomes with high nucleotide identity were widespread, and DWV type A master variants were rare, with an indication that RT-PCR surveillance may over-detect type A due to the prevalence of recombinant DWV genomes. Future work should use genomic strategies to avoid misidentification of common honey bee virus genomes and their impact on colony health.

Beyond the hive: designing an alternative food wax through chemical processes

This study focuses on the development and detailed characterization of a synthetic alternative to natural beeswax, tailored specifically for beekeeping applications. Through a systematic experimental approach, a comprehensive comparison was conducted between the properties of the newly developed biomatrix and those of natural beeswax. The investigation emphasized their physicochemical attributes, aiming to assess the feasibility of the synthetic substitute. The findings reveal that this alternative wax, composed of paraffin, soy wax, natural honey, and carefully selected oils, exhibits spectroscopic and thermal properties that closely resemble those of natural beeswax. The synthetic wax demonstrated significant potential, presenting itself as a practical and innovative solution for beekeeping. Beyond its effectiveness, this substitute offers a sustainable and cost-effective alternative, promoting ecological and economic benefits while ensuring the health and productivity of bee colonies. Such a development could represent a meaningful step forward in supporting sustainable apiculture practices.

Stressdrives premature hive exiting behavior that leads to death in young honey bee (Apis mellifera) workers.

The Western honey bee, Apis mellifera, is an economically important pollinator, as well as a tractable species for studying the behavioral intricacies of eusociality. Honey bees are currently being challenged by multiple biotic and environmental stressors, many of which act concomitantly to affect colony health and productivity. For instance, developmental stress can lead workers to become precocious foragers and to leave the hive prematurely. Precocious foragers have decreased flight time and lower foraging efficiency, which can ultimately lower colony productivity and even lead to colony collapse. In this study, we tested the hypothesis that stress during pupal development can cause young workers to exit the hive prematurely before they are physically able to fly. This premature exiting behavior results in death outside the hive soon thereafter. To determine how various stressors may lead bees to perform this behavior, we subjected workers during the last pupal stage to either cold stress (26°C for 24h), heat stress (39°C for 24h), or Varroa destructor mite parasitization, and compared the rate of premature hive exits between stressed bees and their respective control counterparts. Upon emergence, we individually tagged focal bees in all treatment groups and introduced them to a common observation hive. We then followed tagged bees over time and monitored their survivorship, as well as their likelihood of performing the premature hive exiting behavior. We also dissected the hypopharyngeal glands of all treatment and control bees sampled. We found that significantly more bees in all three treatment groups exited the hive prematurely compared to their control counterparts. Bees in all treatment groups also had significantly smaller hypopharyngeal glands than control bees. Our results suggest that premature hive exiting behavior is driven by stress and is potentially a form of accelerated age polyethism that leads to premature death.

Unveiling genetic defense mechanisms: expression analysis of hym, AmNrx1, and CYP9Q3 genes in Varroa-resistant anatolian honey bees.

The Varroa destructor represents a significant threat to honey bees, leading to substantial yield losses and declines in colony health. Defense behaviors, including grooming (auto and allogrooming), serve as crucial mechanisms against Varroa infestations, yet the genetic basis of these behaviors remains elusive. This study examines the expression levels of hymenoptaecin (hym), neurexin-1 (AmNrx1), and CYP9Q3, potentially associated with defense behavior, in colonies of the Muğla honey bee ecotype (Apis mellifera anatoliaca) subjected to a Varroa selection program. Worker bees from 23 control groups and 23 colonies under selection were evaluated by using qPCR analysis. Results reveal a significant upregulation of hym, AmNrx1, and CYP9Q3 genes in the selected group, with fold changes of 2.9, 2.95, and 3.26 respectively compared to controls (p < 0.01). This suggests that selection against Varroa induces alterations in gene expression linked to Varroa exposure behaviors. These findings advocate for the potential use of hym, AmNrx1, and CYP9Q3 genes in preselection for future Varroa-resistant programs in honey bees. Supported by previous studies, these genes may facilitate the establishment of populations with enhanced defense behaviors, such as autogrooming and allogrooming.

Extensive influence of microsporidian infection on sucrose solution consumption, antioxidant enzyme activity, cell structure, and lifespan of Asian honeybees.

Apis cerana is the original host of Vairimorpha (Nosema) ceranae, a widespread fungal parasite that causes bee nosemosis, which severely threatens the health of bee colonies and the sustainable development of the apiculture industry. To evaluate the impact of V. ceranae infection on A. c. cerana workers, V. ceranae spores were purified and used to inoculate newly emerged workers to evaluate the effects of V. ceranae infection. This was followed by an in-depth investigation of V. ceranae spore load and host sucrose solution consumption. Activities of four major antioxidant enzymes (SOD, PPO, CAT, and GST) were determined. Paraffin sections of the host midgut tissue were prepared and subjected to microscopic observation. The survival rates of V. ceranae-inoculated and uninoculated workers were analyzed. The results showed that spore load gradually increased and peaked at 12 dpi. The consumption of workers in the V. ceranae-inoculated group was extremely significant higher (P < 0.0001) than that of workers in the un-inoculated group. The results of antioxidant enzyme activity were suggestive of positive host defense via catalase (CAT) and glutathione-S-transferase (GST) in the middle stage of infection, as well as the negative fungal impact on superoxide dismutase (SOD) and polyphenol oxidase (PPO) at the whole stage of infection, reflecting the complex host-parasite interaction. Additionally, we observed a disruption in the structure of the host midgut epithelial cells. Moreover, the survival rate of workers in V. ceranae-inoculated groups was nearly always lower than that of workers in the uninoculated groups. These results demonstrate a consistent increase in spore load with the proliferation of V. ceranae, leading to persistent energetic stress and midgut epithelial cell structural damage to the host, ultimately resulting in a shortened lifespan for the host. Our findings enhance the current understanding of the interactions between A. cerana and V. ceranae as well as provide a solid basis for exploring the mechanisms underlying host response and V. ceranae infection.

Exposure to constant artificial light alters honey bee sleep rhythms and disrupts sleep

Artificial light at night (ALAN) changes animal behavior in multiple invertebrates and vertebrates and can result in decreased fitness. However, ALAN effects have not been studied in European honey bees (Apis mellifera), an important pollinator in which foragers show strong circadian rhythmicity. Colonies can be exposed to ALAN in swarm clusters, when bees cluster outside the nest on hot days and evenings, and, in limited cases, when they build nests in the open. We captured and maintained foragers in incubated cages and subjected them to constant light (LL), constant dark (DD), or 12 h light:12 h dark (LD) cycle, and observed them with infrared cameras. After 79 h, there was a significant interaction of treatment and time because LL bees slept less. In detail, the bees maintained a regular sleep pattern for three days but LL bees showed a shift on the fourth day. LL bees had the largest sleep differences from LD controls, with trends of lengthened periods and increased phase misalignment from both LD and DD bees. LL bees also experienced significantly more disturbances from their nestmates and preferred to sleep in the lower portion of the cages, which had significantly lower light intensity. These findings suggest that ALAN can disrupt the sleep of honey bee foragers, which has implications for their behavior and overall colony health.

APPLICATION OF PCR FOR THE DETECTION OF VIRAL INFECTIONS IN THE HONEYBEE Apis mellifera IN THE NORTHERN REGIONS OF UKRAINE

Aim. This study investigated viruses of the western honeybee (Apis mellifera) from the northern regions of Ukraine using polymerase chain reaction (PCR). Methods. To identify honeybee viruses, oligonucleotide primers, which are specific to a gene fragment encoding the capsid protein and a fragment of the RNA-dependent RNA polymerase gene, were used. The reaction mixture contained 12.5 μL of master mix solution (DNA polymerase, dNTPs, and buffer), 1 μL of each primer (20 pmol/μL), 2 μL of cDNA, and water to a total volume of 25 μL. PCR products were visualized in a 2% agarose gel. Results. In the Zhytomyr region in Apis mellifera was revealed for the first time the presence of four viruses representing different taxonomic groups, in particular Israeli Acute Paralysis Virus (IAPV), Sacbrood Virus (SBV), Deformed Wing Virus (DWV) and Chronic Bee Paralysis Virus (CBPV). Three of these viruses (SBV, DWV, and CBPV) were detected in the Kyiv region, while two (SBV and DWV) were identified in the Chernihiv region. Conclusions. The obtained data indicate the widespread distribution of Apis mellifera honeybee viruses in the northern regions of Ukraine. The use of PCR to detect bee viruses will contribute to the development of effective methods for improving the health of bee colonies.

Nurse honey bees filter fungicide residues to maintain larval health

Residues of plant protection products (PPPs) are frequently detected in bee matrices1,2,3,4,5,6 due to foraging bees collecting contaminated nectar and pollen, which they bring back to their hive. The collected material is further used by nurse bees to produce glandular secretions for feeding their larvae.7 Potential exposure to PPPs occurs through direct oral ingestion, contact during foraging, or interaction with contaminated hive material.8,9 Contaminants can pose health risks to adult worker bees,10,11 queens,12,13 drones (males),14 or larvae,15,16 potentially impacting colony health and productivity. However, residue concentrations can vary significantly between analyzed matrices, and potential accumulation or dilution steps have not been widely investigated. Although research has provided valuable insights into contamination risks, there remain gaps in our understanding of the entire pathway from field, via foragers, stored products, nurse bees, and finally to food jelly, i.e., royal, worker, and drone jelly, and the larvae, including all possible processing steps.17 We collected samples of bee-relevant matrices following the in-field spray application of the product Pictor Active, containing the fungicides boscalid and pyraclostrobin. The samples were analyzed for residues along this entire pathway. Fungicide residues were reduced by a factor of 8-80 from stored product to nurse bees' heads, suggesting a filtering function of nurse bees. Furthermore, detected residues in larval food jelly resulted from added pollen and not from nurse bee secretions. Calculated risk quotients were at least twice as low as the threshold values, suggesting a low risk to honey bee colonies from these fungicides at the tested application rate.

Longitudinal Analysis of Honey Bee Colony Health as a Function of Pesticide Exposure

Sixty commercial honey bee colonies were monitored over the course of one year with the goal of assessing potential correlations between measured colony strength and environmental stressors, including exposures to pesticides and pathogens. We developed a new method for assessing colony health by determining the fractional change in population of the four colonies on each pallet between peak population on 1 June and the last population assessment prior to winter on 1 October. This fractional change in population was evaluated as a function of pesticide load per pallet for each of the 37 pesticide chemicals detected, grouping pallets by beekeeper. The analysis of individual chemicals showed that both imidacloprid and cyprodinil were negatively correlated with colony health, while tau-fluvalinate and dinotefuran (at very low concentrations) were positively correlated, possibly because of effects on Varroa control. Exposure to groups of chemicals was also evaluated. Normalization of each chemical concentration to the maximum observed for that chemical provided an equal weighting for each chemical, even though their relative occurrence in the environment and their effective toxicities might differ. A total of 24 chemical groups whose members share a structural commonality, a functional commonality, or suspected synergistic actions were considered, demonstrating negative correlations between colony health and exposures to neonicotinoids as a group and neonicotinoids in combination with (1) methoxyfenozide (2) organophosphates, and (3) diflubenzuron. Analysis of several groups of fungicides applied to almonds during pollination also showed negative correlations with colony health.

Preliminary observations on the physiology and behavior of Sytretomorpha szaboi Papp

Sytretomorpha szaboi Papp (Hymenoptera: Braconidae) is a parasitic wasp that specializes in parasitizing the Apis cerana and is widely distributed in the Eastern Ocean region, particularly in southern China and northern India. The parasitization rate of this parasitic wasp can reach as high as 20%, which poses a significant threat to the health of bee colonies. This study reports the results of observations of S. szaboi in Chongqing, China. Current research on the physiology and behavior of S. szaboi is limited; thus, this report provides a valuable contribution to the existing body of knowledge on this type of parasitic wasp. Consequently, during the summers of 2023 and 2024, we observed the physiological and behavioral characteristics of S. szaboi and their hosts in the field at the Bee Breeding Center of Chongqing, along with additional incubated observations conducted in the laboratory.

The Genetic Characterization of Lake Sinai Virus in Colony Losses Apiaries in Türkiye

Objective: Honeybees (Apis mellifera) have a unique role in natural pollination and maintaining biodiversity in the ecosystem. The alarming increase in unexpected colony losses, mysterious bee deaths, and the tragic extinction of entire colonies (Colony collapsed disorder- CCD) have sounded a global alarm, demanding immediate attention and collaborative action to address these critical challenges in bee breeding. Diseases, parasites, and pathogens significantly threaten colony health. Türkiye is a significant honey producer, providing an ideal environment for beekeeping due to its unique eco-geographical features. Unexpected colony losses and bee deaths are also questions of concern for beekeepers in Türkiye. Material Method: In this study investigated honey bee viruses in apiaries experiencing sudden bee death losses and CCD-like symptoms between 2021 and 2023 in Türkiye, involving genetic analysis of the LSV RdRp gene region. The honeybee and varroa samples were obtained from 52 colonies in 26 apiaries complaining of unexpected bee deaths and CCD-like symptoms between May 2021 and September 2023. Result: The results showed a high DWV, BQCV, and LSV prevalence, respectively. The sampled apiaries were infested mild-moderate- high grade with Varroa mites. Following PCR results, DWV, BQCV, LSV, IAPV, CBPV, and SBV positivity was detected at 69.2% (n=18), 50% (n=13), 38.46% (n=10), 26.9% (n=7), 19.2% (n=5) and 3.8% (n=1), respectively. High rates of multiple virus coexisting and high varroa infestation were noted in colonies with heavy losses and CCD-like complaints. The RdRp gene from two LSV samples (TrLSV-6474, TrLSV-6517) was sequenced. Turkish LSV samples (TrLSVs) showed a 72.88% homology of each other and clustered LSV4 branches in the phylogenetic tree. Turkish LSV sequences showed a closer similarity rate than reference sequences in GenBank with Asian Korean, Chinese, and Japanese LSV sequences. Conclusion: Further investigation is needed to comprehend the implications of elevated LSV populations on colony losses. The execution of genetic research with a more extensive sample size can significantly enhance the demonstration of species diversity and provide valuable insights into the influence of LSV variants on honeybee health and the management of diseases.

Identifying and modeling the impact of neonicotinoid exposure on honey bee colony profit

Abstract Pollination by the European honey bee, Apis mellifera, is essential for the production of many crops, including highbush blueberries (Vaccinum corymbosum). To understand the impact of agrochemicals (specifically, neonicotinoids, a class of synthetic, neurotoxic insecticides) on these pollinators, we conducted a field study during the blueberry blooms of 2020 and 2021 in British Columbia (B.C.). Forty experimental honey bee colonies were placed in the Fraser Valley: half of the colonies were located within 1.5 km of highbush blueberry fields (“near” colonies) and half were located more than 1.5 km away (“far” colonies). We calculated risk quotients for these compounds using their chronic lethal dietary dose (LDD50) and median lethal concentration (LC50). Pesticide risk was similar between colonies located near and far from blueberry forage, suggesting that toxicity risks are regionally ubiquitous. Two systemic neonicotinoid insecticides, clothianidin and thiamethoxam, were found at quantities that exceeded chronic international levels of concern. We developed a profit model for a pollinating beekeeper in B.C. that was parameterized by: detected pesticide levels; lethal and sublethal bee health; and economic data. For colonies exposed to neonicotinoid pesticides in and out of the blueberry forage radii, there were economic consequences from colony mortality and sublethal effects such as a loss of honey production and compromised colony health. Further, replacing dead colonies with local bees was more profitable than replacing them with imported packages, illustrating that beekeeping management selection of local options can have a positive effect on overall profit.

Pollen foraging mediates exposure to dichotomous stressor syndromes in honey bees.

Recent declines in the health of honey bee colonies used for crop pollination pose a considerable threat to global food security. Foraging by honey bee workers represents the primary route of exposure to a plethora of toxins and pathogens known to affect bee health, but it remains unclear how foraging preferences impact colony-level patterns of stressor exposure. Resolving this knowledge gap is crucial for enhancing the health of honey bees and the agricultural systems that rely on them for pollination. To address this, we carried out a national-scale experiment encompassing 456 Canadian honey bee colonies to first characterize pollen foraging preferences in relation to major crops and then explore how foraging behavior influences patterns of stressor exposure. We used a metagenetic approach to quantify honey bee dietary breadth and found that bees display distinct foraging preferences that vary substantially relative to crop type and proximity, and the breadth of foraging interactions can be used to predict the abundance and diversity of stressors a colony is exposed to. Foraging on diverse plant communities was associated with increased exposure to pathogens, while the opposite was associated with increased exposure to xenobiotics. Our work provides the first large-scale empirical evidence that pollen foraging behavior plays an influential role in determining exposure to dichotomous stressor syndromes in honey bees.