Colonization Of Microorganisms Research Articles

Colonization profiles of gut microbiota in goat kids from neonatal to weaning period.

Understanding the colonization and change patterns of gut microbiota is pivotal for comprehending host health. As a newly cultured breed, the studies on the gut microbiota of Tianfu goats remain limited. This study aimed to address this gap by analyzing the microbial composition and colonization patterns of fecal samples collected from goat kids from birth to weaning. Fecal samples were collected on days 0, 7, 14, 21, 28, 35, 42, 49, 53, 55, 57, and 64, and the changes and colonization patterns of microorganisms were analyzed through high-throughput 16S rRNA sequencing. The results showed that the abundance of fecal microbiota in goat kids gradually increased over time, followed by a decrease after weaning and stabilization, with reduced individual differences. The colonization of fecal microorganisms mainly presented three different stages: days 0-14, days 21-49, and days 53-64. During the suckling period, the relative abundance of Proteobacteria (72.34%) was the highest, followed by Firmicutes (21.66%). From 21 days old, the microbiota in goat kids gradually to be diverse, with Lachnospiraceae and Ruminococcaceae being dominant. During post-weaning, Ruminococcaceae (30.98-33.34%) was becoming prominence which helpful for cellulose decomposition. LEfSe analyzed three important time points (d0 vs. d7, d7 vs. d14, d49 vs. d53, LDA score > 4 and p < 0.05), 53 microbial communities with stage differences were identified. Functional prediction using PICRUSt revealed that differential microbial communities are mainly related to carbohydrate and amino acid metabolism pathways. Overall, this study addresses the intricate relationship between ages, diets, and microbiota compositions in Tianfu goat kids, and also offering insights into microorganisms-host interactions.

Stand age-related effects of mangrove on archaeal methanogenesis in sediments: Community assembly and co-occurrence patterns

Mangrove sediment is a key source of methane emissions; however, archaea community structure dynamics and methanogenesis activities during long-term mangrove restoration remain unclear. In this study, microcosm incubations revealed a substantial reduction in microbial-mediated methane production potential from mangrove sediments with increasing stand age; methane production rates decreased from 0.42 ng g−1 d−1 in 6-year-old stands to 0.23 ng g−1 d−1 in 64-year-old stands. High-throughput sequencing revealed a reduction in community diversity because of specific microorganism colonization and species loss, notably a decline in the relative abundance of Bathyarchaeia in sediments of 64-year-old stands. In addition, mangrove sediments, especially those in older stands (20- and 64-year-old), had more complex and stable co-occurrence microbial networks than mudflats. Furthermore, archaea community assembly in older stands was dominated by stochastic processes wherein dispersal limitation was prominent, and that in younger stands (6- and 12-year-old) was driven by deterministic processes. The proportion of dispersal limitation of Bathyarchaeia and traditional methanogens in sediment decreased with an increase in stand age. Quantitative polymerase chain reaction analysis confirmed a decrease in Bathyarchaeia (from 3.50 to 0.54 copies g−1) and mcrA gene (from 3.83 to 0.25 copies g−1) abundance in mangrove sediments with an increase in stand age. These findings demonstrate the critical role of Bathyarchaeia in methanogenesis; the decline in microbial interactions and abundance, and the reduced proportion of dispersal limitation of Bathyarchaeia and traditional methanogens collectively contributed to the mitigation of microbial-mediated methane production potential in older mangrove stands.

Effects of Microbiota on the Soiling Process of PV Modules in Arid Zones of the Atacama Desert

Photovoltaic technology has proven to be a reliable, economical, and clean energy source that is capable of adapting to diverse geographical conditions. However, factors such as soiling overshadow these qualities, thus leading to production losses and affecting the profitability of this technology. For these reasons, soiling is a highly studied topic, which involves considering the physicochemical characterization of the deposited material, mitigation strategies, effect predictions, and cleaning mechanisms. However, there is a relatively unexplored area related to the microbiological contribution to soiling. The surface of photovoltaic modules, along with the deposited material and local atmospheric factors, fosters favorable conditions for the colonization of microorganisms. These microorganisms influence the soiling mechanisms and optical properties of photovoltaic modules. This work presents a detailed characterization of the microbial diversity present in the soiling deposited on photovoltaic modules installed in the Atacama Desert. Two study sites were defined: Antofagasta and the Solar Platform of the Atacama Desert, which have warm and cold desert climates, respectively. Mineralogical characterization tests, heavy metal analyses, TOC, and inorganic element analyses were conducted on the deposited material. Additionally, the culturable isolates and the metagenomic DNA of the soiling samples and biofilms grown on standard PV glass were characterized using next-generation sequencing. The results show that the deposited soiling contained a microbiological component that had adapted to extreme desert conditions. The presence of the genera Arthrobacter, Kocuria, and Dietzia were identified in the culturable isolates from Antofagasta, while Arthrobacter and Dietzia were obtained from the Solar Platform of the Atacama Desert. The metagenomic DNA was mainly represented by the genera Pontibacter, Noviherbaspirillum, Massilia, Arthrobacter, Hymenobacter, and Deinococcus at Antofagasta. However, at the Solar Platform of the Atacama Desert, the analyzed samples presented DNA concentrations below 0.5 ng/µL, which made their preparation unviable. At the PSDA, the biofilms formed by the genera Peribacillus and Kocuria were identified, whereas the UA showed a greater abundance of bacteria that favored biofilm formation, including those that belonged to the genera Bacillus, Sporosarcina, Bhargavaea, Mesaobacillus, Cytobacillus, Caldakalibacillus, and Planococcus. Based on these results, we propose a soiling mechanism that considers the microbiological contribution to material cementation.

Infectious meningitis. Why are the leptomeninges preferentially involved? Electron microscopic insights.

In infectious meningitis, pathogens preferentially attack the leptomeninges (pia mater and arachnoid) rather than the pachymeninges (dura mater). This study aims to provide ultra-anatomical insights from our extensive collection of electron microscopy images and propose mechanisms, highlighting structures that favor the introduction, adherence, colonization, and proliferation of microorganisms leading to spinal meningitis. Over several years, we analyzed an extensive collection of transmission and scanning electron microscopy images of human spinal meninges captured in our laboratories. Upon examining 378 of those images, we identified potential sites for the iatrogenic or hematogenic introduction and adherence of microorganisms, as well as sites for their colonization and proliferation. These included the outer surface of the spinal dural sac, structures within the epidural space, and the spinal dural sac itself, which comprises compact dura mater with interwoven collagen fibers and tightly bound arachnoid cells. Also, the subdural (extra-arachnoid) compartment, consisting of fragile neurothelial cells prone to rupture under force, formed an acquired spinal subdural space, a new subarachnoid compartment, limited by arachnoid trabeculae, that surrounded the nerve roots and spinal cord and the pia mater. Macrophages, fibroblasts, mast cells, and plasma cells were also observed within the dura mater, arachnoid layer, arachnoid trabeculae, and pia mater. These images illustrate how the characteristics of the meningeal layers could contribute to bacterial adhesion and proliferation at various locations, inducing selective inflammation during (iatrogenic) spinal meningitis. In addition, the images help to explain why magnetic resonance imaging enhancement appears preferentially at specific sites.

The effects of invertebrates on wood decomposition across the world.

Invertebrates and microorganisms are important but climate-dependent agents of wood decomposition globally. In this meta-analysis, we investigated what drives the invertebrate effect on wood decomposition worldwide. Globally, we found wood decomposition rates were on average approximately 40% higher when invertebrates were present compared to when they were excluded. This effect was most pronounced in the tropics, owing mainly to the activities of termites. The invertebrate effect was stronger for woody debris without bark as well as for that of larger diameter, possibly reflecting bark- and diameter-mediated differences in fungal colonisation or activity rates relative to those of invertebrates. Our meta-analysis shows similar overall invertebrate effect sizes on decomposition of woody debris derived from angiosperms and gymnosperms globally. Our results suggest the existence of critical interactions between microorganism colonisation and the invertebrate contribution to wood decomposition. To improve biogeochemical models, a better quantification of invertebrate contributions to wood decomposition is needed.

Temporal Investigation of the Maternal Origins of Fetal Gut Microbiota.

In utero colonization or deposition of beneficial microorganisms and their by-products likely occurs through various mechanisms, such as hematogenous spread or ascension from the reproductive tract. With high-throughput sequencing techniques, the identification of microbial components in first-pass neonatal meconium has been achieved. While these components are low-biomass and often not abundant enough to culture, the presence of microbial DNA signatures may promote fetal immune tolerance or epigenetic regulation prior to birth. The aim of this study was to investigate the maternal source of the neonatal first-pass meconium microbiome. Maternal vaginal and anal samples collected from twenty-one maternal-infant dyad pairs were compared via principal component analysis to first-pass neonatal meconium microbial compositions. Results demonstrated the greatest degree of similarity between the maternal gut microbiome in the second and third trimesters and vaginal microbiome samples across pregnancy, suggesting that the maternal gut microbiota may translocate to the fetal gut during pregnancy. This study sheds light on the origin and timing of the potential transfer of maternal microbial species to offspring in utero.

Does the Incorporation of Graphene Oxide into PMMA Influence its Antimicrobial Activity?

The increase in infectious diseases mediated by oral bacteria has been one of the most potent threats to human health, specifically to the elderly. Researchers seek to incorporate antimicrobial nanomaterials into PMMA to control the colonization of microorganisms and reduce deadly diseases. To conduct a systematic review to answer the question: "Does graphene oxide incorporated into polymethylmethacrylate influence its antimicrobial activity?" The electronic search was performed in PubMed, Embase, Web of Science, Scopus, and Google Scholar databases, and articles published until October 2021 were selected. The search terms were: ("acrylic resin" OR PMMA) AND (graphene OR "oxide graphene") AND (antimicrobial OR antibacterial OR "anti-infective agent"). The risk of bias analysis of the articles was performed using the quasi-experimental study assessment tool adapted from the JBI. Two hundred and fifty-nine articles were found in the databases. After removing the duplicates, 245 were analyzed by title and abstract. Of these, 06 were selected for full reading, of which 03 met the eligibility criteria and were included in this systematic review. This systematic review's findings can infer that the incorporation of graphene in the form of graphene-based coatings to PMMA showed antimicrobial effectiveness.

Electrical response and biodegradation of Sepia melanin-shellac films printed on paper

Sepia melanin, a biopigment extracted from the ink sac of cuttlefish, is relevant to sustainable organic electronics. In this work, we flexographically print films from an ink of Sepia melanin including shellac as a bio-sourced binder on silver electrode-patterned paper. We examine the electrical response in high humidity and ambient conditions (here the electronic conductivity is as high as 10−4 S/cm). Additionally, we study the biodegradation of the printed films and their individual constituents based on their mineralization into CO2 under composting conditions. The printed films exhibit biodegradation levels of about 97 ± 25% in 85 d. We observe microorganism colonization on the printed film’s surface. The analysis of the microbial community on the compost reveals that bacterial species within the Acidimicrobiia class, specifically Actinomarinales order, are potentially responsible for the biodegradation of the printed film. Meanwhile, ecotoxicity tests conducted by germinating Lolium multiflorum and Tagetes erecta suggest that printed films have negligible phytotoxicity.

Screening and Characterization of Probiotics Isolated from Traditional Fermented Products of Ethnic-Minorities in Northwest China and Evaluation Replacing Antibiotics Breeding Effect in Broiler.

In this study, Lactobacillus fermentum DM7-6 (DM7-6), Lactobacillus plantarum DM9-7 (DM9-7), and Bacillus subtilis YF9-4 (YF9-4) were isolated from traditional fermented products. The survival rate of DM7-6, DM9-7, and YF9-4 in simulated intestinal gastric fluid reached 61.29%, 44.82%, and 55.26%, respectively. These strains had inhibition ability against common pathogens, and the inhibition zone diameters were more than 7 mm. Antioxidant tests showed these strains had good scavenging capacity for superoxide anion, hydroxyl radical and DPPH, and the total reduction capacity reached 65%. Then DM7-6, DM9-7 and YF9-4 were fed to broilers to study the effects on antioxidant capacity, immune response, biochemical indices, tissue morphology, and gut microbiota. 180 healthy broilers were allocated randomly into six experimental groups. SOD, GSH-Px, and T-AOC in broilers serum were detected, and the results showed probiotics significantly improve antioxidant capacity compared to CK group, while antibiotics showed the opposite result. Besides, IgA, IgM, IgG, TNF-α, and IL-2 indicated it could significantly improve immunity by adding probiotics in broilers diets. However, antibiotics reduced immunoglobulin levels and enhanced inflammation index. Biochemical indicators and tissue morphology showed probiotics had a protective effect on metabolic organs. Gut microbiota analysis proved antibiotics could significantly decrease microbial community diversity and increase the proportion of opportunistic pathogens, while probiotics could improve the diversity of gut microbiota and promote the colonization of beneficial microorganisms. In summary, probiotics DM7-6, DM9-7, and YF9-4 can improve the broiler's health by improving antioxidant capacity and immune function, regulating gut microbiota, and can be used as alternative probiotics for antibiotics-free breeding of broilers.

Novel Nano Drug-Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI.

Catheter-associated urinary tract infection (CAUTI) is a prevalent type of hospital-acquired infection, affecting approximately 15% to 25% of patients with urinary catheters. Long-term use of the catheter can lead to colonization of microorganisms and biofilm formation, and may develop into bacterial CAUTI. However, the frequent replacement of catheters in clinical settings can result in tissue damage, inflammation, ulceration, and additional complications, causing discomfort and pain for patients. In light of these challenges, a novel nanodrug-supported hydrogel coating called NP-AM/FK@OMV-P/H has been developed in this study. Through in vitro experiments, it is confirmed that OMV nano-loaded liquid gel coating has an effective reaction against E.coli HAase and releases antibacterial drugs. This coating has also demonstrated strong inhibition of E.coli and has shown the ability to inhibit the formation of bacterial biofilm. These findings highlight the potential of the OMV nanoparticle gel coating in preventing and treating bacterial infections. Notably, NP-AM/FK@OMV-P/H has exhibited greater efficacy against multidrug-resistant E.coli associated with UTIs compared to coatings containing single antimicrobial peptides or antibiotics. Additionally, it has demonstrated good biosecurity. In conclusion, the NP-AM/FK@OMV-P/H coating holds great potential in providing benefits to patients with CAUTI.

Superiority of native soil core microbiomes in supporting plant growth

Native core microbiomes represent a unique opportunity to support food provision and plant-based industries. Yet, these microbiomes are often neglected when developing synthetic communities (SynComs) to support plant health and growth. Here, we study the contribution of native core, native non-core and non-native microorganisms to support plant production. We construct four alternative SynComs based on the excellent growth promoting ability of individual stain and paired non-antagonistic action. One of microbiome based SynCom (SC2) shows a high niche breadth and low average variation degree in-vitro interaction. The promoting-growth effect of SC2 can be transferred to non-sterile environment, attributing to the colonization of native core microorganisms and the improvement of rhizosphere promoting-growth function including nitrogen fixation, IAA production, and dissolved phosphorus. Further, microbial fertilizer based on SC2 and composite carrier (rapeseed cake fertilizer + rice husk carbon) increase the net biomass of plant by 129%. Our results highlight the fundamental importance of native core microorganisms to boost plant production.

The Human Microbiome-A Physiologic Perspective.

The human microbiome consists of the microorganisms associated with the body, such as bacteria, fungi, archaea, protozoa, and viruses, along with their gene content and products. These microbes are abundant in the digestive, respiratory, renal/urinary, and reproductive systems. While microbes found in other organs/tissues are often associated with diseases, some reports suggest their presence even in healthy individuals. Lack of microbial colonization does not indicate a lack of microbial influence, as their metabolites can affect distant locations through circulation. In a healthy state, these microbes maintain a mutualistic relationship and help shape the host's physiological functions. Unlike the host's genetic content, microbial gene content and expression are dynamic and influenced by factors such as ethnicity, genetic background, sex, age, lifestyle/diet, and psychological/physical conditions. Therefore, defining a healthy microbiome becomes challenging as it is context dependent and can vary over time for an individual. Although differences in microbial composition have been observed in various diseases, these changes may reflect host alterations rather than causing the disease itself. As the field is evolving, there is increased emphasis on understanding when changes in the microbiome are an important component of pathogenesis rather than the consequence of a disease state. This article focuses on the microbial component in the digestive and respiratory tracts-the primary sites colonized by microorganisms-and the physiological functions of microbial metabolites in these systems. It also discusses their physiological functions in the central nervous and cardiovascular systems, which have no microorganism colonization under healthy conditions based on human studies. © 2024 American Physiological Society. Compr Physiol 14:5491-5519, 2024.

The Effectiveness of Belimbing Wuluh Fruit Extract (Averrhoa bilimbi L) as A Smear Layer Cleanser

Background: Root canal treatment is an effort to maintain the function and aesthetics of teeth. The smear layer is formed from the remains of instrumentation composed of organic and inorganic components. A smear layer in the tooth root canal will benefit the adhesion and colonization of microorganisms, block the action of disinfectant solutions, and prevent medicaments in the tooth root canal from penetrating the dentin tubules in the tooth root canal walls. Irrigation can be used for cleaning and disinfection in areas of the root canal that cannot be reached using instruments. Starfruit contains alkoloids, saponins, tannins, flavonoids, phenols, and triterpenoids. Objective: To determine the effectiveness of star fruit extract (Averrhoa bilimbi L) as a smear layer cleaning agent. Materials and Methods: This researcher used laboratory or experimental testing called Post Test Only Control Design. Samples were taken using the purposive sampling technique involving four treatments and six repetitions. The statistical test used is the Mann-Whiteney test. Results: Based on the Mann-Whiteney test, the test results obtained a p-value of 0.093 which is more significant than 0.05 (p-value 0.05). The results showed no significant difference between the 17% EDTA and 35% extract treatment groups regarding smear layer cleanliness. The 35% extract group is as good as the 17% EDTA group. Conclusion: star fruit extract (Averrhoa bilimbi L) is 35% effective in cleaning the smear layer.

Maternal versus environmental contributions to the piglet pioneer microbiome.

The pioneer microbiome is the initial colonization and establishment of microorganisms within the neonate. The objective of this project was to quantify maternal and environmental contributions to the piglet's pioneer microbiome. Sterile swabs were used to collect samples from the gilt's rectum, the farrowing crate before and after gilts were moved in, the gilt's birth canal during farrowing, and the piglet's rectum on days 0 (prior to suckling), 3, and 10 post-farrowing and at weaning (21.6 ± 1.0 days post-farrowing). During farrowing, colostrum was collected from each gilt from a representative sample of teats into a single sterile collection cup. Bacterial DNA extraction and sequencing targeted the V4 hypervariable region of the 16S rRNA gene. The relative abundance of Lactobacillus in the piglet microbiome was lower on day 3 compared to day 0, 10, and at weaning (P < 0.05). For alpha diversity, piglet samples exhibited distinct clustering for bacterial richness by day (P < 0.01). Multiple regression analyses indicated that the birth canal explained 51.6% of the variation observed in the piglet day 0 microbiome (P < 0.0001) and 6.5% of the variation in the piglet day 10 microbiome (P = 0.013). The day 10 microbiome explained 58.6% of the variation observed in the piglet microbiome at weaning (P < 0.0001). Bacterial communities of the farrowing crate and colostrum did not impact the piglet microbiome for any day (P > 0.10). Results indicate that the piglet pioneer microbiome is largely influenced by the microbiome of the birth canal.

Bisphenol A sorption on commercial polyvinyl chloride microplastics: Effects of UV-aging, biofilm colonization and additives on plastic behaviour in the environment.

Chemical additives are important components in commercial microplastics and their leaching behaviour has been widely studied. However, little is known about the potential effect of additives on the adsorption/desorption behaviour of pollutants on microplastics and their subsequent role as vectors for pollutant transport in the environment. In this study, two types of commercial polyvinyl chloride (PVC1 and PVC2) microplastics were aged by UV irradiation and biotic modification via biofilm colonization to investigate the adsorption and desorption behaviour of bisphenol A (BPA). Surface cracks and new functional groups (e.g., O–H) were found on PVC1 after UV irradiation, which increased available adsorption sites and enhanced H‒bonding interaction, resulting in an adsorption capacity increase from 1.28 μg/L to 1.85 μg/L. However, the adsorption and desorption capacity not showed significant changes for PVC2, which might be related to the few characteristic changes after UV aging with the protection of light stabilizers and antioxidants. The adsorption capacity ranged from 1.28 μg/L to 2.06 μg/L for PVC1 and PVC2 microplastics, and increased to 1.62 μg/L—2.95 μg/L after colonization by biofilms. The increased adsorption ability might be related to the N–H functional group, amide groups generated by microorganisms enhancing the affinity for BPA. The opposite effect was observed for desorption. Plasticizers can be metabolized during biofilm formation processes and might play an important role in microorganism colonization. In addition, antioxidants and UV stabilizers might also indirectly influence the colonization of microorganisms’ on microplastics by controlling the degree to which PVC microplastics age under UV. The amount of biomass loading on the microplastics would further alter the adsorption/desorption behaviour of contaminants. This study provides important new insights into the evaluation of the fate of plastic particles in natural environments.

Nano molybdenum trioxide-mediated enhancement of soybean yield through improvement of rhizosphere soil molybdenum bioavailability for nitrogen-fixing microbial recruitment

Molybdenum (Mo) plays a pivotal role in the growth and nitrogen-fixing process of plants mediated by rhizobia. However, the influence of nano‑molybdenum trioxide (MoO3NPs) on soybean growth, rhizosphere bioavailable Mo, and nitrogen-fixing microorganisms remains underexplored. Here, we report that compared with that of ionic Mo and bulk MoO3, the utilization of MoO3NPs (specifically NPs0.05 and NPs0.15) significantly boosted the available Mo content in the rhizosphere soil throughout the seedling (by 21.64 %–101.38 %), podding (by 54.44 %–68.89 %), and mature stage (by 34.41 %–to 45.71 %) of soybean growth. Furthermore, both NPs0.05 and NPs0.15 treatments maintained consistently higher levels of acid-extractable Mo, reducible Mo, and oxidizable Mo across these stages, which facilitated stable conversion and supply of bioavailable Mo. Within the rhizosphere soil, NPs0.05 and NPs0.15 treatments resulted in the highest relative abundance of Rhizobiales and Bradyrhizobium genera, and significantly promoted the colonization of nitrogen-fixing microorganisms, thereby increasing the content of nitrate nitrogen (NO3−-N) by 8.69 % and 7.72 % and ammonium nitrogen (NH4+-N) by 44.75 % and 17.55 %, respectively. Ultimately, these effects together contributed to 107.17 % and 84.00 % increment in soybean yield by NPs0.05 and NPs0.15 treatments, respectively. In summary, our findings underscore the potential of employing MoO3NPs to promote plant growth and maintain soil nitrogen cycling, indicating distinct advantages of MoO3NPs over ionic Mo and bulk MoO3.

MYCOLOGICAL ANALYSIS OF CYSTIC CONTENT IN PATIENTS WITH POLYMICROCYSTIC CHANGES OF THE MAXILLARY SINUS MUCOSA

The prevalence of mycotic infections of the paranasal sinuses is constantly increasing worldwide. Pathogens can enter the sinuses through the respiratory tract that leads to the colonization of microorganisms in both the lungs and paranasal sinuses as part of the normal microflora. The presence of filamentous fungi in the maxillary sinus may result from endodontic treatment of molars directly adjacent to the sinus, increasing the risk of fungal foci development. Fungal paranasal sinusitis manifests in invasive or non-invasive forms. While mycological culture remains the traditional gold standard for mycosis diagnosis, its sensitivity is reportedly low. Paranasal sinus cysts comprise 4-8% of general ENT pathologies and approximately 4-16% of chronic paranasal sinus diseases, with a predominant presence in the maxillary sinus. Factors that contribute to the development of sinusitis and create favorable conditions for fungal spore adhesion and growth include impaired ventilation and transport function of the sinus mucosa, inadequate use of broad-spectrum antibacterial drugs and steroid therapy, as well as household factors. Recently, there has been an increase in the number of cases of multiple small cysts in maxillary sinuses. Clinical manifestations range from asymptomatic incidental findings to varying degrees of discomfort and/or pain localized in the maxillary sinus area. The aim of this study is to identify the involvement of a fungal agent and to develop a set of therapeutic and prophylactic measures for polymicrocystic changes in the mucous membrane of the maxillary sinuses. The study enrolled 37 patients aged 18 to 65 years. Atypical cystic content was collected and cultured in Sabouraud's nutrient broth and Amies transport medium at 35-37°C for 5-7 days, with final measurements on day 21. Treatment involved a 14-day regimen of itraconazole (100 mg once daily) alongside topical miramistin (0.01% solution, 2 ml) sinus injections. The findings obtained demonstrate the following: positive fungal growth was observed in 26 cases (70.3%), predominantly Penicillium (48.6%) and Aspergillus (21.6%). Absence of growth was noted in 11 cases (29.7%). Follow-up examinations in 6 months and in a year revealed no crusts in the nasal cavity, moderate mucous discharge in the sinuses, and no polymicrocystic changes. Conclusion. The analysis has demonstrated the involvement of fungal flora in the development and recurrence of cystic changes in the maxillary sinus mucosa, advocating for mycological examination alongside bacteriological analysis. Itraconazole for systemic therapy and miramistin for topical therapy demonstrate efficacy in treatment and recurrence prevention.

An investigation into the effect of UV irradiation and biofilm colonization on adsorption and desorption behavior of polyurethane (PU) microplastics for bisphenol A (BPA)

Recent studies have demonstrated that microplastics (MPs) can act as a transport vector for pollutants in the environment. However, most studies have focused on carbon-carbon backbone polymers, with little known about heteroatom backbone polymers. In this study, polyurethane (PU) MPs were modified by UV irradiation and microorganism colonization to assess the different aging treatments on the interaction behavior (adsorption/desorption) between PU MPs and bisphenol A (BPA). The results of the adsorption kinetics experiment illustrated that the adsorption process of BPA could be described well by a pseudo-second order kinetic equation on both fresh and aged PU MPs. Although fresh PU MPs revealed the highest saturated adsorption capacity, at low BPA exposure concentrations (≤ 100 μg/L) the adsorption capacities for UV aged and biofilm colonized PU MPs were very similar to fresh MPs or even slightly higher than for fresh MPs, the main adsorption mechanisms including H-bonding interactions and π–π interactions. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) investigations suggested obvious physicochemical property changes (e.g., generation of new functional groups, erosion, etc.) after UV irradiation and biofilm colonization. This study also showed that different exposure environments (ultrapure water, artificial seawater, simulated gastric fluid) could affect BPA adsorption/desorption via hydrophobic and electrostatic interaction on PU surfaces. These results further clarify the adsorption mechanisms of BPA on PU MPs that have undergone different aging treatments, and provides a theoretical basis for the assessment of environmental behavior and exposure risks when PU MPs and BPA coexist.

Exposure to resorcinol bis (diphenyl phosphate) induces colonization of alien microorganisms with potential impacts on the gut microbiota and metabolic disruption in male zebrafish

Organophosphate esters (OPEs) have been demonstrated to induce various forms of toxicity in aquatic organisms. However, a scarcity of evidence impedes the conclusive determination of whether OPEs manifest sex-dependent toxic effects. Here, we investigated the effects of tris (1-chloro-2-propyl) phosphate (TCPP) and resorcinol bis (diphenyl phosphate) (RDP) on the intestines of both female and male zebrafish. The results indicated that, in comparison to TCPP, RDP induced more pronounced intestinal microstructural damage and oxidative stress, particularly in male zebrafish. 16S rRNA sequencing and metabolomics revealed significant alterations in the species richness and oxidative stress-related metabolites in the intestinal microbiota of zebrafish under exposure to both TCPP and RDP, manifesting gender-specific effects. Based on differential species analysis, we defined invasive species and applied invasion theory to analyze the reasons for changes in the male fish intestinal community. Correlation analysis demonstrated that alien species may have potential effects on metabolism. Overall, this study reveals a pronounced gender-dependent impact on both the intestinal microbiota and metabolic disruptions of zebrafish due to OPEs exposure and offers a novel perspective on the influence of pollutants on intestinal microbial communities and metabolism.

Microbial Adhesion to Different Thermoplastic Denture Base Materials in Kennedy Class I Partially Edentulous Patients.

Introduction Since the polished and fitting surface of the denture base may promote the colonization of microorganisms, it is essential to know how the different types of denture bases prevent or encourage the adhesion of microorganisms. This study aimed to compare the microbial adhesion to the polished and fitting surfaces of thermoplastic nylon, thermoplastic acetal, and thermoplastic acrylic denture bases in Kennedy Class Ⅰ, partially edentulous patients. Materials and methods Thirteen patients were included in the study. The group consisted of eight males (61.54%) and five females (38.46%), with an age range of 41-50 years (mean age 46.1 years). Three types (groups) of removable partial dentures will be made for each patient using different thermoplastic denture base materials: thermoplastic nylon; thermoplastic acetal; and thermoplastic acrylic. The polished and fitting surfaces of the denture bases were swabbed after a one-month follow-up period. Microbial adhesion was evaluated by counting the microorganisms' colony-forming units (CFU) in the collected specimens. The data were collected and statistically analyzed. Results The study revealed no statistically significant difference in microbial adhesion to both polished and fitting surfaces between all types of studied thermoplastic denture base materials. However, the results showed that for the polished surface, the microbial adhesion median of thermoplastic acrylic denture base (40.5 CC x 102/ml) was higher than that of thermoplastic acetal (29.0 CC x 102/ml) and thermoplastic nylon (16.0 CC x 102/ml). Regarding the fitting surface, the microbial adhesion median of thermoplastic acrylic (51.0 CC x 102/ml) is higher than that of thermoplastic acetal (41.0 CC x 102/ml) and thermoplastic nylon (23.0 CC x 102/ml). Conclusion The thermoplastic nylon denture base materials showed less microbial adhesion among the studied thermoplastic materials, so it may be recommended to be used as a denture base material for individuals at high risk of denture stomatitis.