Electroacoustic techniques are vital tools in order to characterize colloidal suspensions. When an acoustic wave is applied to a source of colloidal suspension, a vibration potential is generated as a result of the interaction. This particular responded potential is termed Colloid Vibration Potential (CVP) and is detected as an AC electrical signal. We address the physicochemical properties upon measuring the CVP for Silica Dioxide (SiO2) colloidal particles with three different concentrations of 0.5, 1 and 5% by wt., and a Particle Size Distribution (PSD) ranging from 35, 70, 100, 165 and 190nm. Our experimental technique has relatively eased access to the colloidal source and in addition provides an improved signal to noise ratio with implementation of a fully external detector to sense the generated responded signal. Current study provides relatively consistent measurements at three different excitation frequencies 0.5, 1 and 2MHz. Specifically, the high concentration (5%) of all samples with different particle size distribution enhanced the CVP signal magnitude, facilitating the highest value of 0.0378mA obtained for 100nm at 0.5MHz. The CVP increases initially as a function of the PSD but descends at a particle size > 100nm. Finally, for the case of the excitation frequency the CVP magnitude yields highest value at 0.5MHz.