PDF HTML阅读 XML下载 导出引用 引用提醒 高温胁迫下仿刺参表观遗传调控相关基因的表达特征 DOI: 作者: 作者单位: 1. 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室, 山东 烟台 264006;2. 上海海洋大学 水产与生命学院, 上海 201306 作者简介: 李尚俊(1993-),男,硕士研究生.E-mail:lishangjun1993@163.com 通讯作者: 中图分类号: S917 基金项目: 国家自然科学基金项目(31602144);山东省自然科学基金项目(ZR2013CM029);山东省现代农业产业技术体系项目(SDAIT-22-02);黄河三角洲学者岗位;山东省良种工程 Characteristics of epigenetic regulation of related genes under high-temperature stress in sea cucumber Apostichopus japonicus Author: Affiliation: 1. Shandong Marine Resources and Environment Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:以仿刺参(DNMT1)、组蛋白去乙酰化酶(histone deacetylase,)为目的基因,采用实时荧光定量PCR(qRT-PCR)技术和相对定量2-ΔΔactin为内参进行校正,比较了高温胁迫下3个基因在呼吸树、消化道、体液、肌肉各组织中的表达情况。以15℃条件下的刺参为对照组,其目的基因表达量为基准1,在体液中,<0.01),21~24℃时上升不明显(基因在24℃时的表达量显著(<0.05),之后随着温度升高其表达量稍有升高,分别在30℃得到最大表达量为2.90和3.19。总体来看,各基因随着温度的升高其表达量都增高,上调表达趋势明显,在30℃达到最高表达量。对比3个基因在不同组织中的表达差异,体液中表达变化较明显,在24℃以后均大于2.5;肌肉中最低,在整个高温胁迫过程中基因表达变化量小于2。在30℃的温度胁迫条件下,检测在0 h、3 h、6 h、9 h、12 h、15 h 6个时间点3个基因的表达量,以0 h各基因的表达量为基准1,结果显示,3个基因的表达量都表现为上调表达,除消化道的<0.05);胁迫9 h以后,各目的基因表达量变化不明显或稍有降低(>0.05)。不同组织中目的基因的表达趋势一致,体液中3种表观相关基因的表达量最高值高于其他3种组织。本研究为从表观遗传修饰角度解析刺参的高温逆境应答反应机制奠定了基础。 Abstract:Epigenetic regulation of the related genes DNA methyltransferase (DNMT1), histone deacetylase (HDAC3), and histone methyltransferase (MLL5) were used to analyze gene-expression quantity in the respiratory tree, intestine, coelomic fluid, and muscle tissue of the sea cucumber under high-temperature stress conditions. The housekeeping gene of the sea cucumber was used for internal calibration, and the relative quantification 2-ΔΔmethod was employed using real-time fluorescent quantitative PCR. Sea cucumber kept at <15℃ were used as a control group, and the target gene-expression quantity of this group was used as benchmark-1. Increased expression of the DNMT1 gene was insignificant in the coelomic fluids at 18℃, but the expression rose significantly between 18℃ and 21℃, thereafter the increase was less acute, until the expression quantity reached a maximum of 3.26 at 30℃. The expression quantity of the MLL5 gene and the HDAC3 gene were significant at 2℃, and then the expression quantity increased slightly as the temperature increased, reaching a maximum of 2.90 and 3.19, respectively, at 30℃. Overall, the expression quantity of the three genes increased with increasing temperatures, and this trend was obvious, reaching a maximum at 30℃. Comparisons of different tissues showed diverse expression quantities of the three genes. Expression of the genes was prominent in the coelomic fluids, at >2.5, above 24℃, while the expression quantity of the three genes in muscle tissue was <2 in the whole spectrum of high temperatures. Under the stress conditions of 30℃, the gene-expression quantity of the three genes was detected at 0 h, 3 h, 6 h, 9 h, 12 h and 15 h, and the expression quantity of every gene at 0 h was used as benchmark-1. The data showed that the expression quantities of the three genes were characterized by increasing expression with the extension of stress time during 0 to 9 h (<0.05), except expression of HDAC3 in intestine stabilized at about 6 h. The expression quantity was either not obviously or only slightly lower after 9 h. In addition, the trend in expression quantity was consistent in the different tissues, yet the maximum expression quantity of every gene was highest in the coelomic fluids than in any of the other tissues. This expression analysis of epigenetic-related genes under high temperatures lays a foundation for interpreting the mechanism of response to high-temperature stress in sea cucumber from the viewpoint of epigenetic regulation. 参考文献 相似文献 引证文献