Urinary Collagen Research Articles

Automated analysis of the pyridinium crosslinks of collagen in tissue and urine using solid-phase extraction and reversed-phase high-performance liquid chromatography

A fully automated method for assaying the collagen crosslinking amino acids, pyridinoline and deoxypyridinoline, in human urine samples or tissue hydrolysates is described. Samples were processed using a Gilson ASPEC system with solid-phase extraction of the cross-links on columns containing 100 mg of microgranular cellulose. Introduction of an additional solvent step during sample preparation allowed direct analysis by reversed-phase HPLC and elimination of the drying step used previously in a manual method. Use of a synthetic pyridinoline derivative as internal standard enabled accurate quantification of the crosslinks by correcting for recoveries through the whole assay. Samples were analyzed in sequential mode with a total assay time of 30 min. The automated assay showed close correlation with the manual method for both free and total crosslink determinations in human urine ( r > 0.97). Reproducibility was improved, as seen from replicate analyses of human urine (CV < 3% for automated pyridinoline measurement compared with 8–12% previously observed for the manual method). Crosslink excretion is the most useful marker of collagen degradation in metabolic bone diseases and arthritic disorders. The automated assay which has been developed is rapid, convenient, and reliable and will greatly facilitate the monitoring of urinary collagen crosslinks and their tissue levels in clinical investigations.

An immunoassay to urinary collagen crosslinks

An immunoassay to urinary collagen crosslinks

Pyridinium crosslinks as markers of bone resorption in patients with breast cancer

Collagen breakdown, and thus bone resorption, can now be assessed by measuring the urinary excretion of the collagen crosslinks, pyridinoline (Pyd) and deoxypyridinoline (Dpd). In a pilot study we measured Pyd and Dpd in 20 patients with breast cancer, ten with known bone metastases and ten with no recognised metastases in bone or elsewhere after 1 year's subsequent follow up. Eight out of the ten patients with metastases had crosslink excretion values higher than the reference interval, but so did some patients without known metastatic disease. For both crosslinks there was a clear correlation with serum alkaline phosphatase activity measured at about the same time. We consider that measurement of urinary collagen crosslink assays may have a place in the early detection of metastatic spread to bone.

Urinary excretion of pyridinoline crosslinks correlates with bone turnover measured on iliac crest biopsy in patients with vertebral osteoporosis

Vertebral osteoporosis, a common disorder in elderly women, is characterized by a wide spectrum of bone turnover abnormalities on iliac crest biopsy. The level of bone formation can be assessed noninvasively by measuring serum osteocalcin, whereas conventional biochemical markers of bone resorption lack specificity and do not reflect bone resorption assessed from histology. We measured the urinary excretion of pyridinoline crosslinks Pyr and D-Pyr, a specific marker of bone and cartilage collagen degradation, along with serum osteocalcin and urinary hydroxyproline, in 36 elderly women with vertebral osteoporosis who had a simultaneous iliac crest biopsy. Urinary pyridinoline crosslinks, but not hydroxyproline, correlated significantly with histologic resorption, assessed by the osteoclast surface (r = 0.35, p less than 0.05 for Pyr; r = 0.46, p less than 0.01 for D-Pyr). In addition, Pyr and D-Pyr were correlated with the bone formation rate as well as serum osteocalcin, with correlation coefficients ranging from 0.69 to 0.80, p less than 0.0001. These data indicate that Pyr and D-Pyr are sensitive markers of bone turnover in elderly women with vertebral osteoporosis. The poor correlation between the level of urinary collagen crosslinks and histological assessment of bone resorption indicates the low sensitivity of iliac crest histomorphometry in the measurement of resorption rate of the skeleton.

Toxicological effects of an organophosphorus pesticide (dimethoate) on urinary collagen metabolites in normal and high protein diets fed female albino rats

The effect of an organophosphorus pesticide (dimethoate) on the urinary excretion of hydroxyproline (total, nondialysable, dialysable and free fractions) and hydroxylysylglycosides, glucosylgalactosyl hydroxylysine and galactosehydroxylysine was investigated in two groups of female albino rats fed with normal and high protein diets. In comparison to controls, dimethoate treated animals were found to excrete significantly decreased amounts of urinary hydroxyproline fractions from 7th day onwards. The excretion of total hydroxylysylglycoside in urine parallels the excretion of hydroxyproline. The urinary output of both glu-gal-hyl and gal-hyl was also appreciably lower from dimethoate treated animals. The normal ratio of glu-gal-hyl and gal-hyl found in the urine of dimethoate treated animals was discussed in light of decreased turn over of collagen in both bone and skin. The effect of dimethoate in rats fed with high protein diet was comparatively less than those fed with normal diet.

Excretion of urinary collagen metabolites correlates to severity of pulmonary disease in cystic fibrosis.

The urinary excretion of collagen metabolites (hydroxylysine, hydroxyproline and proline) was significantly increased in 10 patients with cystic fibrosis and chronic broncho-pulmonary Pseudomonas aeruginosa infection as compared with 14 age matched controls. The increase was significantly correlated to impaired pulmonary function (FVC and FEV1). The results indicate that urinary collagen metabolites reflect degradation of lung connective tissue and may be an indicator of the severity of pulmonary disease in cystic fibrosis. Parts of the excreted hydroxyproline may be degradation products of elastin.

Analaysis by high performance liquid chromatography of two hydroxypyridinium cross-links of collagen in urine as indices of disease activity in rheumatoid arthritis

Analaysis by high performance liquid chromatography of two hydroxypyridinium cross-links of collagen in urine as indices of disease activity in rheumatoid arthritis

Quantitative analysis of the pyridinium crosslinks of collagen in urine using ion-paired reversed-phase high-performance liquid chromatography

A method has been developed for the simultaneous and rapid analysis of the 3-hydroxypyridinium crosslinks of mature collagen, pyridinoline and deoxypyridinoline, in samples of urine and tissue. After hydrolysis in 6 m HCl, samples were prefractionated by partition chromatography using columns packed with cellulose CF1. The appropriate fractions were freeze-dried and then subjected to high-performance liquid chromatography using a gradient system with 20 m m NH 4Cl, pH 3.5, and acetonitrile, incorporating 1-octanesulfonic acid as an ion-pairing agent; the pyridinium crosslinks were detected fluorometrically. The limit of detection of both crosslinks was 1 pmol. The mean recoveries of added standard to samples ranged between 94.2 and 97.2%, and the reproducibility of the complete procedure was between 12 and 16%. An application of the method in the study of degenerative disorders of bone and connective tissue is illustrated.

Studies on prolidase deficiency with a possible defect in collagen metabolism.

Skin collagen of a female patient with prolidase deficiency was examined for the distribution of borohydride-reducible cross-links and the proportion of type III to type I collagen. Patient's skin contained after reduction more dihydroxylysinonorleucine relative to hydroxylysinonorleucine and type III collagen than expected for normally matured skin. These findings suggest that collagen of the patient's skin failed to follow a time-related normal maturation process and the collagen metabolism was disturbed. The composition of urinary collagen metabolites was also unusual. On the the other hand, her asymptomatic brother with prolidase deficiency showed the normal urinary compositon of collagen matabolites. It is suggested that prolidase deficiency and defect in collagen metabolism independent of it are both responsible for clinical manifestation.

Variation of urinary acid glycosaminoglycan and collagen metabolite excretion with disease activity in generalized scleroderma

Follow-up of patients with generalized scleroderma improving during treatment with collagen biosynthesis inhibitors showed a decrease towards normalization of the elevated urinary values for acid glycosaminoglycans and collagen catabolites. The determinations supply objective evidence of the disease activity as well as of the efficacy of treatment. In the control group, relatively high urinary values for the two macromolecules were found in the second decade of life in comparison with older age groups.

Urinary excretion of hydroxyproline, hydroxylysine and hydroxylysine glycosides by patients with paget's disease of bone and carcinoma with metastases in bone

Patients with carcinoma metastases in bone and Paget's disease of bone have different patterns of collagen metabolite excretion. Both forms of bone disease resulted in an increased excretion of total hydroxyproline and the ratio of glucosylgalactosylhydroxylysine to galactosylhydroxylysine was below normal. The excretion of glucosylgalactosylhydroxylysine and galactosylhydroxylysine was increased in all patients with carcinoma metastases in bone while the excretion of glucosylgalactosylhydroxylysine in patients with Paget's disease was normal and the excretion of galactosylhydroxylysine was increased only in patients with more active Paget's disease. The ratio of total hydroxylysine (free hydroxylysine + glycosylated hydroxylysines) to total hydroxyproline was normal in patients with carcinoma metastases in bone and below normal in patients with Paget's disease of bone. The pattern of urinary collagen metabolite excretion is a more specific indicator of the presence of bone disease than is the measurement of the excretion rate of any individual collagen metabolite. Bone diseases of different etiologies may result in different patterns of urinary collagen metabolite excretion.

Cartilage atrophy.

HomeRadiologyVol. 112, No. 1 PreviousNext Diagnostic RadiologyCartilage AtrophyWinford H. Pool, Jr.Winford H. Pool, Jr.Author Affiliations Department of Radiology Medical College of GeorgiaAugusta, Ga. 30902Winford H. Pool, Jr.Published Online:Jul 1 1974https://doi.org/10.1148/112.1.47MoreSectionsPDF ToolsImage ViewerAdd to favoritesCiteTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinked In AbstractTwo hundred cases of flaccid paralysis of the lower extremities were reviewed, and in 25 the cartilaginous space of the hip joint was found to be narrowed by at least 50%. It is postulated that the cartilage reduction is the result of atrophy following an altered nutrition associated with lack of stress and a decrease in the production of synovial fluid.Article HistoryPublished in print: July 1974 FiguresReferencesRelatedDetailsCited ByCartilage abnormalities and osteophytes in the fingers of elite sport climbers: An ultrasonography-based cross-sectional studyTorstenPastor, StefanFröhlich, JörgSpörri, TonjaSchreiber, AndreasSchweizer2020 | European Journal of Sport Science, Vol. 20, No. 2Mechanoadaptation: articular cartilage through thick and thinTonia L.Vincent, Angus K. T.Wann2019 | The Journal of Physiology, Vol. 597, No. 5Pediatric Orthopedic ImagingElkaMiller, NagwaWilson2015Avaliação radiográfica de quadris de pacientes lesados medularesJeanGrynwald, Juliana de Melo LafaieteBastos, Vinicius Basañez AleluiaCosta, Carolina de MedeirosRimkus, AlbertoCliquet Júnior2012 | Acta Ortopédica Brasileira, Vol. 20, No. 1Contrasting alteration patterns of different cartilage plates in knee articular cartilage after spinal cord injury in ratsHMoriyama, KNishihara, MHosoda, YSaka, NKanemura, KTakayanagi, OYoshimura, YTobimatsu2009 | Spinal Cord, Vol. 47, No. 3Skeletal unloading induces a full-thickness patellar cartilage defect with increase of urinary collagen II CTx degradation marker in growing ratsMasatoTomiya, KyosukeFujikawa, ShoichiIchimura, ToshiyukiKikuchi, YasuoYoshihara, KoichiNemoto2009 | Bone, Vol. 44, No. 2Bone and Joint ImagingDonaldResnick, Mark J.Kransdorf2005Longitudinal analysis of cartilage atrophy in the knees of patients with spinal cord injuryB.Vanwanseele, F.Eckstein, H.Knecht, A.Spaepen, E.Stüssi2003 | Arthritis & Rheumatism, Vol. 48, No. 12Knee cartilage of spinal cord-injured patients displays progressive thinning in the absence of normal joint loading and movementB.Vanwanseele, F.Eckstein, H.Knecht, E.Stüssi, A.Spaepen2002 | Arthritis & Rheumatism, Vol. 46, No. 8The effects of immobilization on the characteristics of articular cartilage: current concepts and future directionsB.Vanwanseele, E.Lucchinetti, E.Stüssi2002 | Osteoarthritis and Cartilage, Vol. 10, No. 5Effects of continuous distraction on cartilage in a moving joint: An investigation on adult rabbitsShih-ChiehHung, KozoNakamura, RyojiShiro, KiyokazuTanaka, HazimeKawahara, TakahideKurokawa1997 | Journal of Orthopaedic Research, Vol. 15, No. 3Unweighting Accelerates Tidemark Advancement in Articular Cartilage at the Knee Joint of RatsKim M.O'Connor1997 | Journal of Bone and Mineral Research, Vol. 12, No. 4Degenerative joint abnormalities in patients with paraplegia of duration greater than 20 yearsE JWylie, T M HChakera1988 | Spinal Cord, Vol. 26, No. 2Articular cartilage atrophy in lower limb amputeesC.Benichou, J. M.Wirotius1982 | Arthritis & Rheumatism, Vol. 25, No. 1Cartilage Atrophy Following Spinal Cord DamageJ.ANDERSON, P.BREIDAHL1981 | Australasian Radiology, Vol. 25, No. 1Recommended Articles RSNA Education Exhibits RSNA Case Collection Vol. 112, No. 1 Metrics Altmetric Score PDF download

Correlation of Urinary Collagen IV: Urinary Albumin Ratio in the Prediction of Preeclampsia

The purpose of this study was to assess the value of MA in the prediction of PE and also to compare urinary collagen IV with MA in this prediction. A total of 200 subjects (age 25±4 years, M±SC), were selected in their early pregnancy (10 to 14 weeks). Blood glucose, serum lipids, serum and urinary creatinine, urinary albumin and urinary collagen IV were measured in these subjects and they were followed up to the term for the possible development of PE, MA was defined by albumin creatinine ratio (ACR) above 32mg/g and high urinary collagen IV was defined by values above the cut-off point 2.54 ng/ml determined by the median value of the controls. The data were analyzed by grouping the subjects who developed PE in later stages of pregnancy (the PE group) and those who did not develop PE in later stages (the control group). Out of 200 subjects 94 were primigravida and 106 were multigravida. From the total subjects 16 developed PE, which shows a prevalence of about 8.4%. The PE group showed a relatively higher value of ACR as compared to control group. 106 subjects had MA out of which 13 developed PE. The sensitivity of ACR in predicting the development of PE was 80%, specificity 49.54%, PPV 12.69% and NPV 96.42%. Regarding urinary collagen IV the PE group showed no significant difference with the value of the control group. 103 subjects had high urinary collagen IV and out of them 11 developed PE. The sensitivity of high urinary collagen IV in prediction PE was found to have no significant difference with ACR in this respect. The data suggest the following conclusions:Early pregnancy levels of microalbuminuria and urinary collagen IV can be used as predictors of PE with high negative value.Urinary collagen IV has no added advantage over MA in this respect. Keywords : MA-Micro DOI: 10.3329/uhj.v5i2.4555 University Heart Journal Vol.5(2) July 2009 pp.59-62

Urinary free proline and collagen metabolites in newborns, healthy children, and in collagen diseases

The urinary output of free and peptide-bound imino acids was studied in 41 newborns and 48 children aged 3–14 years; 20 of these latter suffered from various collagen diseases. Quantitative data about free urinary proline are given. The relationship between free and total urinary proline as well as hydroxyproline and plasma proline was studied. The urinary output of total and free imino acids was increased from the 1st through the 15th day of life. There was a significant decrease in the urinary output of both free and total imino acids in children with collagen disease, though plasma proline levels were normal.

Tissue composition of rats exposed to chronic centrifugation.

ARTICLESTissue composition of rats exposed to chronic centrifugationJ Oyama, and B ZeitmanJ Oyama, and B ZeitmanPublished Online:01 Nov 1967https://doi.org/10.1152/ajplegacy.1967.213.5.1305MoreSectionsPDF (1 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByCan Vestibular Stimulation be Used to Treat Obesity?7 January 2019 | BioEssays, Vol. 41, No. 2A noninvasive analysis of urinary musculoskeletal collagen metabolism markers from rhesus monkeys subject to chronic hypergravityD. A. Martinez, P. E. Patterson-Buckendahl, A. Lust, K. M. Shea-Rangel, T. M. Hoban-Higgins, C. A. Fuller, and A. C. Vailas1 October 2008 | Journal of Applied Physiology, Vol. 105, No. 4In a hypergravity environment neonatal survival is adversely affected by alterations in dam tissue metabolism rather than reduced food intakeLaura M. Lintault, Elzbieta I. Zakrzewska, Rhonda L. Maple, Lisa A. Baer, Theresa M. Casey, April E. Ronca, Charles E. Wade, and Karen Plaut1 June 2007 | Journal of Applied Physiology, Vol. 102, No. 6Effects of 2 G on adiposity, leptin, lipoprotein lipase, and uncoupling protein-1 in lean and obese Zucker ratsL. Elisabeth Warren, Barbara A. Horwitz, Jock S. Hamilton, and Charles A. Fuller1 February 2001 | Journal of Applied Physiology, Vol. 90, No. 2Altered Behaviour in Hamsters Conceived and Born in HypergravityBrain Research Bulletin, Vol. 43, No. 3Enhanced skeletal muscle insulin sensitivity in year-old rats adapted to hypergravityC. E. Mondon, C. B. Dolkas, and J. Oyama1 May 1981 | American Journal of Physiology-Endocrinology and Metabolism, Vol. 240, No. 5The relationships among physical, geometrical and mechanical properties of bone, with a note on the properties of nonhuman primate bone12 October 2012 | American Journal of Physical Anthropology, Vol. 23, No. S1On structural theories of basal metabolic rateJournal of Theoretical Biology, Vol. 80, No. 4GRAVITY AS A BIOCHEMICAL DETERMINANTRemodeling of the avian fracture callus by estrone treatment and exposure to a 2 g environmentAnatomy and Embryology, Vol. 154, No. 2Effects of increased earth gravity and estrone treatment on intact and healing avian radiiCalcified Tissue Research, Vol. 23, No. 1Effect of chronic centrifugation on bone density of the ratAnatomy and Embryology, Vol. 151, No. 2Effect of chronic centrifugation on the structural development of the musculoskeletal system of the ratAnatomy and Embryology, Vol. 149, No. 1Comparative Physiology of BoneAn effect of gravity on the postnatal development of the human and rat femurZeitschrift f�r Anatomie und Entwicklungsgeschichte, Vol. 143, No. 2Changes in functional construction of bone in rats under conditions of simulated increased gravityZeitschrift f�r Anatomie und Entwicklungsgeschichte, Vol. 139, No. 3Influence of Gravity on the Maintenance Feed Requirement of ChickensThe Journal of Nutrition, Vol. 101, No. 1Phyysiological Targets of Artificial Gravity: Adaptive Processes in Bone More from this issue > Volume 213Issue 5November 1967Pages 1305-1310 Copyright & PermissionsCopyright © 1967 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1967.213.5.1305PubMed6054882History Published online 1 November 1967 Published in print 1 November 1967 Metrics