Coleus Forskohlii Briq Research Articles

High-frequency shoot regeneration, assessment of genetic fidelity, and histochemical analysis of forskolin production in Coleus forskohlii Briq.

Forskolin, a diterpenoid found in the roots of Coleus forskohlii, has generated significant interest in the medical field due to its various therapeutic uses. This study aimed to establish an effective system for regenerating C. forskohlii plants, ensuring a year-round supply of plant material and forskolin production. We tested different concentrations of cytokinins, either alone or combined with auxin, to see their impact on shoot multiplication and growth. We found that a medium supplemented with 1.5mg L-1 of meta-topolin (mT) resulted in the highest number of shoots (~ 12.66) and leaves (~ 20) within about 5days. When mT (1mg L-1) was combined with a low amount of auxin (0.05mg L-1 NAA), we obtained an even greater number of leaves (~ 23). The shoot regeneration capacity was consistent over five subculture passages, showing minimal variation in mean shoot length and number. During acclimatization, around 91% of the plantlets grown in vermiculite + sand survived. The photosynthetic pigment concentration in the plantlets modestly increased in the first 10days and reached its highest level after 30days. Genetic fidelity assays using inter simple sequence repeats (ISSRs) confirmed the similarity between the in vitro derived plantlets and the mother plant. Micro-morphological features of in vitro and ex-vitro acclimated plantlets also matched those of the mother plant, further confirming genetic accuracy. Histochemical staining with vanillin confirmed the presence of forskolin in the in vitro roots, indicated by the violet coloration in the cells. Forskolin quantification was also validated by HPLC where in vitro derived roots were documented to undergo an almost ~ 1.8-fold in comparison to that of the mother plant. This established protocol can effectively address resource scarcity for commercial-scale forskolin production and sustainable conservation techniques.

Seasonal diversity & spaciotemporal distribution of fungal endophytes associated with the medicinal plant Coleus forskohlii Briq.

Fungi that colonize the healthy tissues of the plants without showing any disease symptoms in the host plants are termed as fungal endophytes. The presence of fungal endophytes provides a positive effect on the host’s growth & development and also triggers the production of some essential bioactive compounds in the host. This study was undertaken to isolate, identify and understand the spaciotemporal distribution and seasonal diversity of fungal endophytes associated with the leaf, stem & root of Coleus forskohlii, an important and endangered medicinal plant. Sampling was done for a period of 12 months between May 2020–April 2021. A total of 950 fungal endophytes were isolated from a total of 1680 tissues of the leaf, stem & root of C. forskohlii. The fungi were identified based on their morphological features and some of them were identified by molecular identification by 18S rRNA sequencing. The endophytic isolates belonged to 10 different orders belonging to 3 different classes-Sordariomycetes (Hypocreales, Xylariales, Microascales, Trichosphaeriales, Glomerellales & Sordariales), Dothiomycetes (Pleosporales, Capnodiales, Botryosphaeriales) & Eurotiomyctes (Eurotiales). About 81.26% of the isolates belonged to Ascomycota & 2.63% of the isolates belonged to Mucoromycota. Chaetomium globosum, Collariella bostrychodes, C. robusta, Colletotrichum gloeosporioides, Fusarium chlamydosporum, Sterile hyaline mycelia, Aspergillus niger, Xylaria curta, X. grammica, Mucor circinelloides & Trichoderma harizianum were the frequently isolated species of fungi. C. globosum, C. bostrychodes, C. gloeosporioides, sterile hyaline mycelia & X. curta were found distributed in all the tissues of the plant. C. forskohlii has thus revealed a rich diversity of fungal endophytes that could be isolated & cultured to yield some pharmacologically important bioactive compounds.

Green synthesis, characterization, anti-SARS-CoV-2 entry, and replication of lactoferrin-coated zinc nanoparticles with halting lung fibrosis induced in adult male albino rats

The ethanolic extract of Coleus forskohlii Briq leaves was employed in the green synthesis of zinc nanoparticles (Zn-NPs) by an immediate, one-step, and cost-effective method in the present study. Zn-NPs were coated with purified bovine lactoferrin (LF) and characterized through different instrumental analysis. The biosynthesized Zn-NPs were white in color revealing oval to spherical-shaped particles with an average size of 77 ± 5.50 nm, whereas LF-coated Zn-NPs (LF-Zn-NPs) revealed a larger particles size of up to 98 ± 6.40 nm. The biosynthesized Zn-NPs and LF-Zn-NPs revealed negatively charged surfaces with zeta-potentials of – 20.25 ± 0.35 and – 44.3 ± 3.25 mV, respectively. Interestingly, the LF-Zn-NPs showed potent in vitro retardation for SARS-CoV-2 entry to host cells by binding to the ACE2-receptor and spike protein receptor binding domain at IC50 values of 59.66 and μg/mL, respectively. Additionally, the results indicated the ability of LF-Zn-NPs to inhibit SARS-CoV-2 replication by interfering with RNA-dependent RNA polymerase “RdRp” activity at IC50 of 49.23 μg/mL. In vivo, the LF-Zn-NPs displayed a protective and therapeutic activity against induced pulmonary fibrosis in Bleomycin-treated male albino rats owing to its anti-inflammatory, antioxidant, and significant reduction in CRP, LDH, ferritin, and D-dimer levels. The obtained findings offer a promising route for biosynthesized Zn-NPs and LF-Zn-NPs as promising candidates against COVID-19.

Study on Chemotypic Variability of Coleus forskohlii Briq., Samples Collected from Different Phytogeographical Locations of India and Evaluation of Its Inhibitory Potential.

Coleus forskohlii Briq. is an important medicinal herb, endowed with a wide range of medicinal properties against the variety of ailments. Seven germplasm of C. forskohlii collected from different phyto-geographical locations and identification of elite chemotype was performed with the help of high performance thin layer chromatography. Data of soil analysis correlated with the bioactive compounds and inhibitory potential of the species. Quantification of forskolin and its isomer (iso-forskolin) content were done in all the collected samples of C. forskohlii, which revealed a wide range of variations, varying from 1.15-0.004% and 0.0091 to 0.1077% per dry weights basic, respectively. Variation in the bioactive content may be due to the soil nature and environmental factors. Soil analysis of collected samples demonstrated that there is significant variation in available NPK and micronutrient content and may be reasoned for existing chemotypic variability. In vitro biological activity (antioxidant and antidiabetic) analyses were performed, which reveals that germplasms have a high amount of forskolin and iso-forskolin, both show more activity. The aim of this study was to elucidate the effect of elicitors and precursors on the production of bioactive compounds and identification of best elite germplasm among the populations, to provide basic lead to the industry for commercial exploitability including its location-specific commercial cultivation.

ASSESSMENT OF THE ANTIOXIDANT POWER OF IN VITRO OBTAINED COLEUS FORSKOHLII BRIQ

In thе prеsent studу, a simрle protосоl for in vitro rooting induction of the important medicinal plant Coleus forskohlii Briq with a high content of secondary metabolites with antioxidant activity was described. For the in vitro germination seeds of C. forskohlii were placed on MS medium suррlemented with 0.4 mg L-1 gibberellic acid and 1.0 mg L-1 CaCl2. The оbtаinеd shoots were cultured on a half-strength ½ MS medium containing indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L-1. The maximum rooting of plants (95%) with mean values of 9.2 roots per explant, root length (5.1 cm) and root fresh weight (0.085 g) was obtained on half-strength MS medium with 0.1 mg L-1 IAA after three weeks of culture. The plants were successfully ex vitro adapted with 93.3% survival on the mixture substrate of soil, peat and sand (2:1:1 v/v/v). The antioxidant enzymes асtivitiеs (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) showed the highest values when IBA was used for C. forskohlii in vitro cultivation. Applied auxins influenced the level of secondary metabolites synthesis. Half strength MS culture medium supplemented with 0.5 mg L-1 IAA caused the highest total phenol and flavonoid content as well as the highest antioxidant activity level (radical scavenging activity and ferric reducing antioxidant power). The results indiсаte thаt orgаnоgenеsis is а very соmplicated biologiсаl prосеss involving up and downregulation of plant enzyme and non-enzyme antioxidant protection systems, which plау an imроrtant role during the organogenesis of plants.

ASSESSMENT OF THE ANTIOXIDANT POWER OF IN VITRO OBTAINED COLEUS FORSKOHLII BRIQ

In thе prеsent studу, a simрle protосоl for in vitro rooting induction of the important medicinal plant Coleus forskohlii Briq with a high content of secondary metabolites with antioxidant activity was described. For the in vitro germination seeds of C. forskohlii were placed on MS medium suррlemented with 0.4 mg L-1 gibberellic acid and 1.0 mg L-1 CaCl2. The оbtаinеd shoots were cultured on a half-strength ½ MS medium containing indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L-1. The maximum rooting of plants (95%) with mean values of 9.2 roots per explant, root length (5.1 cm) and root fresh weight (0.085 g) was obtained on half-strength MS medium with 0.1 mg L-1 IAA after three weeks of culture. The plants were successfully ex vitro adapted with 93.3% survival on the mixture substrate of soil, peat and sand (2:1:1 v/v/v). The antioxidant enzymes асtivitiеs (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) showed the highest values when IBA was used for C. forskohlii in vitro cultivation. Applied auxins influenced the level of secondary metabolites synthesis. Half strength MS culture medium supplemented with 0.5 mg L-1 IAA caused the highest total phenol and flavonoid content as well as the highest antioxidant activity level (radical scavenging activity and ferric reducing antioxidant power). The results indiсаte thаt orgаnоgenеsis is а very соmplicated biologiсаl prосеss involving up and downregulation of plant enzyme and non-enzyme antioxidant protection systems, which plау an imроrtant role during the organogenesis of plants.

First Report of Fusarium wilt of Coleus forskohlii Caused by Fusarium oxysporum in China.

Coleus forskohlii (Wild) Briq. is an aromatic plant in the Lamiaceae family cultivated primarily in India, Sri Lanka, Nepal and China (Yunnan Province). This herb is considered to have medicinal properties and the whole plant can be used to treat asthma, cancer and other diseases with remarkable efficacy. Due to the high medicinal and economic value of C. forskohlii, it has been introduced to Tongcheng (N29°18'12.24″, E113°53'59.36″), Hubei Province for cultivation. However, severe Fusarium wilt disease of C. forskohlii has been epidemic in Tongcheng since 2018 with a disease incidence of 5 to 30% in surveyed fields. This disease is characterized typically by root rot, vascular discoloration and leaf wilting of C. forskohlii (Fig 1), resulting in progressive plant death. Ten diseased plants were collected from the fields and the roots and stems were rinsed in 70% ethanol for 5 min and samples at the junction of disease and healthy tissues (0.5 × 0.5 cm2) were cutted and placed on potato dextrose agar (PDA) for fungal isolation in a dark chamber at 28°C. Eventually, ten pure isolates were obtained from hyphal-tip followed by single-spore purification on PDA. Seven of the purified isolates showed white aerial mycelium initially and secreted orange-brown pigment 8 days after incubation. Macroconidia were falciform, hyaline, three to five septate, ranging from 2.02 to 4.17 (mean 2.98 µm) × 10.05 to 21.90 µm (mean 12.04 µm) in size (n = 30) (Fig 2). These morphological characteristics resembled Fusarium oxysporum. (Leslie and Summerell 2006) and we selected one of them for molecular identification. Genome DNA was extracted from isolate (RS-4) using the CTAB method (Mahadevakumar et al. 2018). The translation elongation factor 1 alpha (EF-1α) DNA sequence was amplified using primers EF1/EF2 (Geiser et al. 2004), and the second largest subunit of RNA polymerase II (RPB2) DNA sequence was amplified using primers fRPB2-5F/fRPB2-7cR (Liu et al. 1999). The obtained EF-1α sequence of RS-4 (MW219142) showed 100% identity with that of F. oxysporum (FD_01376) (FUSARIUM-ID database). RPB2 sequences of RS-4 (MW219143) showed 100% identity with F. oxysporum (FD_01679) (FUSARIUM-ID database). Moreover, a phylogenetic tree of the EF-1α gene sequence of RS-4 was constructed based on the Neighbor-Joining method in MEGA7 software (Tamura et al. 2013) and revealed that strain RS-4 was closest to F. oxysporum (Fig 2). To test the pathogenicity of RS-4, six healthy leaves of C. forskohlii were collected and inoculated either with the colonized PDA discs (diameter, 5 mm) of RS-4 or control PDA discs, in a moist chamber at 25 ± 2°C. Five days later, brown-black lesions were observed on all inoculated leaves. However, the non-inoculated leaves were maintained asymptomatic. For in vivo pathogenicity test, twenty-day-old C. forskohlii plants (n=3) were inoculated with 106 spores/ml of the RS-4 at a position approximately 1 cm above the soil. Three seedlings treated with sterile water were used as controls. These inoculated and control seedlings were incubated in a moist chamber (25 ± 2 °C, RH 85%). Three days later, typical Fusarium rot symptoms were observed on all inoculated seedlings with rotten stems and withering leaves (Fig 2). Fungal pathogens were re-isolated from the inoculated sites of in vitro and in vivo inoculations by repeating the above isolating operation, and were reconfirmed through morphological features. This is the first report of F. oxysporum causing root rot on C. forskohlii in China. F. oxysporum is one of the most economically important fungal pathogens causing vascular wilt on a wide range of plants worldwide (Dean et al. 2012). The identification of F. oxysporum as the causal agent of the observed Fusarium wilt on C. forskohlii, is critical to the prevention and control of this disease in the future. Acknowledgement This research was supported by funding from the Key project at the central government level titled, "The ability to establish sustainable uses for valuable Chinese medicinale resources" (2060302) Reference Dean, R., et al. 2012. Mol. Plant. Pathol. 13: 414. https://doi.org/10.1111/j.1364-3703.2011.00783.x. Geiser, D. M., et al. 2004. Eur. J. Plant Pathol. 110: 473. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0. Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Oxford, U.K. Liu, Y. J., et al. 1999. Mol. Biol. Evol. 16: 1799. https://doi.org/10.1093/oxfordjournals.molbev.a026092 Mahadevakumar, S. et al. 2018. Eur. J. Plant Pathol. 151:1081. https://doi.org/10.1007/s10658-017-1415-2. Tamura, K., et al. 2013. Mol. Biol. Evol. 30: 2725. https://doi.org/10.1093/molbev/msw054.

Quorum sensing modulatory and biofilm inhibitory activity of Plectranthus barbatus essential oil: a novel intervention strategy.

The essential oil (EO) from the roots of Plectranthus barbatus Andr. (Syn. Coleus forskohlii Briq.) was evaluated for quorum sensing (QS) inhibitory activity. P. barbatus EO was screened for inhibition of QS regulated violacein production in Chromobacterium violaceum (ATCC 12472) wild-type strain. At inhibitory (6.25% v/v) and sub-inhibitory concentrations (3.125% v/v) of the EO, dose-dependent response in the inhibition of violacein production was observed in C. violaceum. Similarly, sub-MIC (6.25% v/v) of P. barbatus EO disrupted QS regulated biofilm formation by 27.87% and inhibited swarming and twitching motility in Pseudomonas aeruginosa PA01 implying its anti-infective and QS modulatory activity. Fluorescence microscopy studies confirmed the disruption of biofilm formation by EO in P. aeruginosa PAO1. Promising antibacterial activity was recorded at concentrations as low as 3.12% v/v for Listeria monocytogenes (ATCC 13932) and at 6.25% v/v for both Salmonella enterica subsp. enterica serovar Typhimurium (ATCC 25241) and Escherichia coli (ATCC 11775). Furthermore, significant dose-dependent inhibition was observed for biofilm formation and motility in all the tested pathogens in different treated concentrations. GC-MS analysis revealed α-pinene, endo-borneol, bornyl acetate, 1-Hexyl-2-Nitrocyclohexane as the major phytoconstituents. P. barbatus EO or its constituent compounds with QS modulatory, antimicrobial and biofilm inhibitory property could be potential new-age dietary source based intervention and preservation technologies.

EFFECT OF INTEGRATED NUTRIENT MANAGEMENT ON GROWTH PARAMETERS OF MEDICINAL COLEUS (COLEUS FORSKOHLII BRIQ.)

Medicinal coleus (Coleus forskohlii Briq.) is one of the commercial Indian medicinal herb. It plays an important role in medicinal industry. The demand of tuberous roots is very high, but the productivity is very low. Therefore the integrated nutrient management could help in achieving higher growth in medicinal coleus. Thus, an experiment was conducted to know the “Effect of Integrated nutrient management on growth of medicinal coleus (Coleus forskohlii Briq.)” at Sirukalathur village, Ariyalur District - Tamilnadu during the year 2018-2020. A field experiment was consist of different combination of nutrients viz., recommended dose of fertilizers, farmyard manure, neem cake, castor cake and bio-fertilizers (Azospirillum and Azotobacter). The experiment was laid out in randomized block design with 10 treatments and three replications. Among these various treatments tried, plants supplied with T5 (75 % RDF + FYM @ 15 t ha-1 + Castor cake @ 2 t ha-1 + Azotobacter @10 kg ha-1) was recorded maximum growth parameters viz., plant height, number of branches plant-1, plant spread, number of leaves plant-1, leaf area plant-1, LAI, fresh and dry weight of leaves and shoots plant-1 and dry matter production

EFFECT OF INTEGRATED NUTRIENT MANAGEMENT ON YIELD PARAMETERS OF MEDICINAL COLEUS (COLEUS FORSKOHLII BRIQ.)

Medicinal coleus is one of the most important medicinal crop in India. It plays an important role in medicinal industries. The demand of tuberous roots are high but the production is low. Therefore, the research was conducted to know the “Effect of Integrated nutrient management on yield of medicinal coleus (Coleus forskohlii Briq.)” at Sirukalathur village, Ariyalur District – Tamilnadu during 2018-2020. The experiment was laid out in randomized block design with ten treatments and three replications. The plants are treated by using different combination of organic manures (Fym, neem cake and castor cake) and inorganic fertilizers (NPK) along with bio-fertilizers (Azospirillum and Azotobacter). Among these various treatments the plants treated by T5 (75 % RDF + FYM @ 15 t ha-1 + Castor cake @ 2 t ha-1 + Azotobacter @10 kg ha-1) is influenced the yield attributes of medicinal coleus like, maximum number of tuberous roots plant-1, root length, root girth and fresh and dry weight of tuberous roots ha-1

Effect of Glomus fasciculatum On Nutrient Uptake and Growth of a Medicinal Plant, Coleus forskohlii

Aims: Coleus forskohlii Briq. (syn. Coleus barbatus Benth.) belonging to the family Lamiaceae is a well known plant throughout the country and known as ‘pashanbhedi’ in Sanskrit and ‘pathatchur’ in Hindi is one of the most potential medicinal plants of the future, as its pharmacoproperties have been discovered only recently. Its tuberous roots are found to be rich source of forskolin which is being used as a remedy for hypertension, glaucoma, asthma, congestive heart failures and certain types of cancers and also being use as vegetables. In addition, forskolin is reported to have used in the preparation of medicines for controlling body weight, preventing hair greying and restoring normal colour of grey hairs. Its foliage is also employed in treating intestinal disorders and used as a condiment since a long time. Keeping the above views in mind, the present investigation was undertaken to study the influence of an arbuscular mycorrhizal (AM) fungus, G. fasciculatum on phosphorus uptake and growth of Coleus forskohlii.
 Study Design: Various tests or experiments were done in this study. Total leaf tissue phosphorus content was determined following the Microkjeldhal method of Jackson (1973) and chlorophyll estimation was done following the method of Mahadevan and Sridhar (1982) .Root colonization of C. forskohlii by G. fasciculatum was observed by the ‘Rapid clearing and staining technique’ (Phillips & Hayman, 1970). Forskolin content in C. forskohlii tubers was estimated by High performance liquid chromatography (HPLC).
 Place and Duration of Study: Sample: The experiment was carried out in the Department of Botany, Burdwan University. A soil culture of Glomus fasciculatum was propagated as endomycorrhizal species in Zea mays as suitable host in pot culture using sterile sand and soil (pH 5.6; organic carbon 0.6%, P content 45 ppm) in 1:1 ratio. 400 gm of rhizosphere soil with AM-inoculated root fragments of Zea mays was introduced in the dry, loamy, fine-textured soil (pH 5.8; organic carbon 0.6%, P content 45 ppm, total nitrogen 0.03%) of pot culture (2.0 kg of soil per pot) at a depth of 2 inches below the Coleus cutting (12-16 cm long comprising 3-4 pairs of leaves) being planted, between June 2011 and July 2012.
 Results: Responses of an arbuscular mycorrhizal (AM) fungus, Glomus fasciculatum on phosphorus uptake by the plant at different developmental phases have been studied. The result indicates that symbiotic association of mycorrhizal fungus amounts to greater uptake of phosphorus and increased chlorophyll content in AM treated plants than in non mycorrhizal plants which gradually happened to be maintained throughout their developmental stages. Different growth parameters like plant height, root length, no. of roots, no. of leaves, no. of branches, fresh weight of shoot, roots, tubers and forskolin content were significantly higher in AM treated plants than in respective controls.
 Conclusion: The better growth of mycorrhizal plants was attributed to improved nutrient uptake, especially phosphorus. The result suggested that G. fasciculatum is one of the most potentially effective bio-fertilizer of Coleus forskohlii.

Bioinoculants and AM fungus colonized nursery improved management of complex root disease of Coleus forskohlii Briq. under field conditions

Nursery treatment with efficient microbial inoculants, using vermicompost as rooting medium, is not popular technique and underutilized for the management of complex root diseases (involved phyto-pathogens: Fusarium, Ralstonia and Meloidogyne) of Coleus forskohlii. To find a probable profound solution of the above problem we designed an experiment to study the potential of efficient bioinoculants and AM fungus as nursery treatment, where the vermicompost was added as rooting and microbial inoculants multiplying medium, for effective management of complex root disease of C. forskohlii for higher root yield, forskolin content and also to sustain the health of soil. Nursery (cuttings of C. forskohlii) treated with efficient microbial inoculants Pseudomonas monteilii (PM) and Glomus intraradices (GI) showed maximum control efficacy for root-rot/wilt (complex disease) of C. forskohlii and improved the growth, yield attributes and also the content of forskolin in the plants. The efficient bioinoculant P. monteilii (strain CRC1) and AM fungus (G. intraradices) thus proved to be a potent/novel antagonist and can be better utilised for biological management of complex root disease of C. forskohlii under organic field conditions.

Anatomical Changes Linked Lerformance of Two Indigenous Medicinal Plants, Withania Somnifera Dunal and Coleus Forskohlii Briq. Exposed to Supplemental Ultraviolet-B Radiation

Anatomical Changes Linked Lerformance of Two Indigenous Medicinal Plants, Withania Somnifera Dunal and Coleus Forskohlii Briq. Exposed to Supplemental Ultraviolet-B Radiation

Chemotaxonomic variation in forskolin content and its correlation with ecogeographical factors in natural habitat of Coleus forskohlii Briq. collected from Vidarbha (Maharashtra, India)

Phytogeographical variation has an impact on up/down regulation of secondary metabolites in medicinal plants. In the same context, present study was planned to evaluate the variation of forskolin content in Coleus forskohlii from Vidarbha region (Maharashtra), India having different topographical conditions. The chemotypic variation was further correlated with existing soil profile. The data revealed the similarity in morphological, macroscopic and microscopic characters of collected germplasms but there is significant (p<0.05) variation in the content of chemical marker, forskolin. Physicochemical and phytochemical parameters are within the limits of API and serve as standard for commercial utility of samples. NBC-29 (Narkhed) and NBC-31 (Katol) are the first two high forskolin yielding samples, which are clustered together on the basis of forskolin content with similar soil profiles. Furthermore our study demonstrated that there is significant variation in available NPK and micronutrient content within the soil samples and may be reasoned for existing chemotypic variability in samples. Among the micronutrients, manganese and copper exhibit the positive correlation with forskolin.Thus, elite chemotype of C. forskohlii were identified among the samples collected from Vidarbha region and express the relevance of phyto-geographical conditions on the quantity of industrially viable metabolites, forskolin. This aids in site specific exploration of high metabolite yielding accession/samples to meet out the commercial demands and, supply and to promote the agriculture practices of this industrial crop for societal upliftment in area having similar phytogeographical locations.

Effect of Coleus forskohlii and its major constituents on cytochrome P450 induction

Coleus forskohlii Briq. has been used traditionally for the treatment of several ailments since antiquity in Ayurveda. In the present study, an approach has been made to evaluate the effect of C. forskohlii and its major constituents on cytochrome P450 (CYP3A, CYP2B, and CYP2C) mRNA expression in rat hepatocytes. To gain better understanding of the herb–drug interaction potential of the chemical constituents present in C. forskohlii, the extract was subjected to column chromatography followed by standardization with respect to forskolin, 1-deoxyforskolin, and 1,9-dideoxyforskolin using reversed-phase high-performance liquid chromatography (RP-HPLC). Hepatocytes were treated with extracts, fractions, and phytoconstituents, followed by extraction and purification of total mRNA. Study of mRNA expression was carried out through reverse transcription polymerase chain reaction, followed by agarose gel electrophoresis. Results revealed that the test substances did not show any significant mRNA expression compared to the control against CYP3A, CYP2B, and CYP2C. Positive controls such as dexamethasone and rifampin showed significantly high (p < 0.001) induction potential compared to the control. It can be concluded that C. forskohlii and its major constituents may not be involved in CYP450 induction-based drug interaction.

Quality control aspects of herbs and botanicals in developing countries: Coleus forskohlii Briq a case study.

Objective:Current trend of commercialization of herbal medicines draw a huge need of maintaining their quality. The declaration of quality, safety and efficacy of medicinal plants as well as poly-herbal formulations has become an important issue. Hence, qualitative and quantitative analysis of herbal drugs and formulations viz., fingerprint profiles and quantification of the various markers become key factors of quality control.Materials and Methods:Present investigation is a detailed report for quality control of well-known herb Coleus forskohlii Briq, which includes physicochemical parameter determination, safety evaluation, microscropical evaluation, and chromatographic fingerprinting as well.Results:Physico-chemical characters were evaluated according to Indian Pharmacopoeia, further microscopic evaluation of transverse section of Coleus reveals that periderm, secondary phloem, and wide secondary xylem cylinder, which occupies major portion of the root fragmentary. Chromatographic fingerprint profiles of Coleus have been generated, and a marker based standardization strategy was adopted; using different analytical technique like high-performance thin layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectroscopy to maintain quality and ensure safety as well as efficacy.Conclusion:These advancements in modern techniques of analysis can lead to effective quality control of Coleus as well as other herbs. Present report can act as pioneer for quality control of modern herbal medicine.

High Frequency and Rapid in vitro Plant Regeneration of Coleus forskohlii Briq.

Coleus forskohlii Briq. (Lamiaceae) is an important medicinal plant with excellent export potential in herbal drug trade. The pharmacological properties of Coleus forskohlii makes it useful to cure high blood pressure, spasmolysis, obesity and constipation, asthma and certain cancers. The present work is aimed to develop a protocol for minimizing time duration to get complete plants through tissue culture technique. Within 35 ± 2 d using shoot tips as explants; we developed complete plants of Coleus forskohlii in MS medium containing 2.0 mg/l BAP through direct multiplication at the rate of 15-20 shoots per explant. The rooting occurred in all the plants and survival rate of full grown plants is 100%. This protocol is very efficient for the mass multiplication of Coleus forskohlii and is the fastest method as compared to all the reported ones.

Ethnopharmacological and phytomedical knowledge of Coleus forskohlii: An approach towards its safety and therapeutic value

Coleus forskohlii Briq. (Family: Lamiaceae) is a fleshy perennial aromatic herb with fibrous roots that grows under tropical to temperate areas in India, Burma, Thailand, Nepal, Pakistan, Sri Lanka, East Africa and Brazil. The rootstock of the plant is used in Ayurveda and other systems of medicine for several ailments such as heart and lung conditions, asthma, digestive disorder, insomnia, muscle spasm, convulsion and skin diseases. Extensive literature survey was performed based on distribution, morphology, phytochemistry and pharmacological aspects of C. forskohlii through pubmed, scopus, SciFinder, google scholar, sciencedirect and ethnobotanical books. Phytopharmacological knowledge and cosmeceutical use of C. forskohlii and its bioactive constituents have been documented. Coleus roots are exclusive source of forskolin, commonly known as fat burning molecule. It’s unique property is activating of almost all hormone sensitive adenylate cyclase enzymes by cyclic 3′,5′-adenosine monophosphate (cAMP). Other potent compounds are 9-deoxyforskolin (1), 1,9-dideoxyforskolin (2), forskolin I (7), forskolin J (8), forskolin L (9), isoforskolin (16), 1-acetylforskolin (17), forskoditerpenoside A (19), forskoditerpenoside B (20), coleol (21), coleosol (22), 3-hydroxyforskolin (25) etc. Besides well established food and traditional use, clinical evidence indicates its safety assessment and can be taken without any toxic effect. This review covers up to date ethnopharmacological, phytochemical and phytomedical information of C. forskohlii approaching towards its safety and therapeutic perspective.

In vitro Plant Regeneration through Somatic Embryogenesis in Medicinally Important leaf explants of Coleus forskohlii Briq.

In vitro Plant Regeneration through Somatic Embryogenesis in Medicinally Important leaf explants of Coleus forskohlii Briq.

In vitro Plant Regeneration through Somatic Embryogenesis in Medicinally Important leaf explants of Coleus forskohlii Briq.

High-frequency somatic embryogenesis and complete plant regeneration from leaf explants of Coleus forskohlii Briq had been established. The influence of plant growth regulators (PGRs) on the frequency of somatic embryogenesis and plant regeneration were studied. Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or both with 6-benzylaminopurine (BAP), were superior for somatic embryogenesis. Primarily, embryogenic callus was initiated from leaf explants of young plant on MS medium supplemented with 2,4-D (1.0mgl -1 ), 3% sucrose and 0.9% agar in three weeks. Embryogenic callus transferred on 2,4-D and BAP-supplemented medium favored induction of embryos in advance as compared to that on 2,4-D alone supplemented medium. The highest frequency (80 %) of direct somatic embryogenesis was obtained in the combination of BAP (1.0 mg/l) and 2,4-D (0.5 mg/l). Eighty percent of the embryos underwent maturation and conversion to plantlets upon transfer to half-strength MS medium having α-naphthaleneacetic acid (NAA), with BAP. Embryo-derived plantlets were transferred onto specially made plastic cup containing soilrite followed by their transfer to the garden soil. Survival rate of the plantlets under ex vitro condition was 80%.