Abstract
Coleus forskohlii Briq. (Lamiaceae) is an important medicinal plant with excellent export potential in herbal drug trade. The pharmacological properties of Coleus forskohlii makes it useful to cure high blood pressure, spasmolysis, obesity and constipation, asthma and certain cancers. The present work is aimed to develop a protocol for minimizing time duration to get complete plants through tissue culture technique. Within 35 ± 2 d using shoot tips as explants; we developed complete plants of Coleus forskohlii in MS medium containing 2.0 mg/l BAP through direct multiplication at the rate of 15-20 shoots per explant. The rooting occurred in all the plants and survival rate of full grown plants is 100%. This protocol is very efficient for the mass multiplication of Coleus forskohlii and is the fastest method as compared to all the reported ones.
Highlights
Coleus forskohlii Briq. is a herbaceous plant with annual stem and perennial root stock of the mint family (Lamiaceae), with a strong, camphor-like odor that was first discovered in the lower elevations of India
Coleus forskohlii is an important plant in Indian Ayurvedic medicine because its roots produce a labdane diterpenoid, forskolin [2]
Different explants like leaf lamina, node and shoot tip of C. forskohlii were cultured on different concentration of auxins (IAA and IBA) and cytokinins (BAP and Kinetin)
Summary
Coleus forskohlii Briq. is a herbaceous plant with annual stem and perennial root stock of the mint family (Lamiaceae), with a strong, camphor-like odor that was first discovered in the lower elevations of India. Is a herbaceous plant with annual stem and perennial root stock of the mint family (Lamiaceae), with a strong, camphor-like odor that was first discovered in the lower elevations of India. It grows at 600-1800 m elevation on sun-exposed hill slopes and plateaus in arid and semiarid climatic zones. Coleus forskohlii is an important plant in Indian Ayurvedic medicine because its roots produce a labdane diterpenoid, forskolin [2]. Much efficient work has not been done in developing the protocol for tissue culture of Coleus forskohlii. In the present work an appropriate and efficient protocol was developed from shoot tips and nodes, focusing on the identification of effective hormone combinations for rapid multiplication of shoots and their rooting within a considerably shorter period than previously reported
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