Cold Water Extract Research Articles

Recent advances in the quality control of kava, the traditional beverage of the Pacific Islands

Kava, the traditional beverage of the South Pacific Islands is prepared by cold water extraction of the fresh or dried roots and stumps of Piper methysticum Forst. (Piperaceae). Nutraceutical and pharmaceutical kava-based products manufactured in Europe from extracts obtained by solvents (acetone or ethanol) have been implicated in hepatotoxicity cases. Kava has been banned in Germany and, although the German Health Authorities have lost their case in the administrative court, the ban has produced a worldwide negative impact on kava's reputation as a safe product. Quality standards and control are now needed to enable the registration under the FAO/WHO Codex Alimentarius. The present paper reviews the most recent advances in analytical techniques (HPLC, NIRS, DNA analysis using SSRs and DArTs and HP-TLC) to detect unsuitable cultivars and raw material. These techniques are accurate and reliable but they are comparatively expensive and cumbersome for local traders and exporters in the South Pacific. A simple colorimetric test has been developed and represents a fairly convenient and cost efficient way of assessing the quality of traded kava. Possible strategies for improving the industry are discussed.

South African medicinal plant extracts active against influenza A virus

BackgroundInfluenza infection remains a major health threat for animals and humans which crucially requires effective antiviral remedies. The usage of herbal medications as readily available alternatives for their compatibility with the body and fewer side effects compared to synthetic chemical treatments has become popular globally. The aim of this study was to investigate and screen in vitro anti-influenza activity of extracts of five South African medicinal plants, namely Tabernaemontana ventricosa, Cussonia spicata, Rapanea melanophloeos, Pittosporum viridiflorum and Clerodendrum glabrum, species which are used traditionally for the treatment of several diseases such as inflammatory and respiratory diseases.MethodsMethanol, ethanol (100% and 30%), acetone, hot and cold water extracts of the powdered plants leaves were obtained by standard methods. The cytotoxicity was determined by the MTT colorimetric assay on MDCK cells. The concentrations below CC50 values were tested for antiviral activity against influenza A virus (IAV) in different combination treatments. The effect of extracts on viral surface glycoproteins and viral titer were tested by HI and HA virological assays, respectively.ResultsBased on the applied methods, the most effective results against IAV were obtained from Rapanea melanophloeos methanol leaf extract (EC50 = 113.3 μg/ml) and Pittosporum viridiflorum methanol, 100% and 30% ethanol and acetone leaf extracts (EC50 values = 3.6, 3.4, 19.2, 82.3 μg/ml, respectively) in all types of combined treatments especially in pre- and post-penetration combined treatments with highly significant effects against viral titer (P ≤ 0.01).ConclusionThe outcomes offer for the first time a scientific basis for the use of extracts of Rapanea melanophloeos and Pittosporum viridiflorum against IAV. It is worth focusing on the isolation and identification of effective active compounds and elucidating the mechanism of action from these species. However, Tabernaemontana ventricosa, Cussonia spicata and Clerodendrum glabrum leaf extracts were ineffective in vitro in this study.

Bronchodilator effects of Lignosus rhinocerotis extract on rat isolated airways is linked to the blockage of calcium entry

BackgroundLignosus rhinocerotis (Cooke) Ryvarden is a popular medicinal mushroom used for centuries in Southeast Asia to treat asthma and chronic cough. The present study aimed to investigate the effect of this mushroom on airways patency. Materials and methodsThe composition of L. rhinocerotis TM02 cultivar was analyzed. Organ bath experiment was employed to study the bronchodilator effect of Lignosus rhinocerotis cold water extract (CWE) on rat isolated airways. Trachea and bronchus were removed from male Sprague-Dawley rats, cut into rings of 2 mm, pre-contracted with carbachol before adding CWE into the bath in increasing concentrations. To investigate the influence of incubation time, tissues were exposed to intervals of 5, 15 and 30 min between CWE concentrations after pre-contraction with carbachol in subsequent protocol. Next, tissues were pre-incubated with CWE before the addition of different contractile agents, carbachol and 5-hydroxytrptamine (5-HT). The bronchodilator effect of CWE was compared with salmeterol and ipratropium. In order to uncover the mechanism of action of CWE, the role of beta-adrenoceptor, potassium and calcium channels was investigated. ResultsComposition analysis of TM02 cultivar revealed the presence of β-glucans and derivatives of adenosine. The extract fully relaxed the trachea at 3.75 mg/ml (p < 0.0001) and bronchus at 2.5 mg/ml (p < 0.0001). It was observed that lower concentrations of CWE were able to fully relax both trachea and bronchus but at a longer incubation interval between concentrations. CWE pre-incubation significantly reduced the maximum responses of carbachol-induced contractions (in both trachea, p = 0.0012 and bronchus, p = 0.001), and 5-HT-induced contractions (in trachea, p = 0.0048 and bronchus, p = 0.0014). Ipratropium has demonstrated a significant relaxation effect in both trachea (p = 0.0004) and bronchus (p = 0.0031), whereas salmeterol has only affected the bronchus (p = 0.0104). The involvement of β2-adrenoceptor and potassium channel in CWE-mediated airway relaxation is ruled out, but the bronchodilator effect was unequivocally affected by influx of calcium. ConclusionsThe bronchodilator effect of L. rhinocerotis on airways is mediated by calcium signalling pathway downstream of Gαq-coupled protein receptors. The airway relaxation effect is both concentration- and incubation time-dependent. Our findings provide unequivocal evidence to support its traditional use to relieve asthma and cough.

Inhibition of Protein Glycation by Tiger Milk Mushroom [Lignosus rhinocerus (Cooke) Ryvarden] and Search for Potential Anti-diabetic Activity-Related Metabolic Pathways by Genomic and Transcriptomic Data Mining.

Naturally occurring anti-glycation compounds have drawn much interest in recent years as they show potential in reducing or preventing the risk of chronic complications for diabetic patients. In this study, annotation of the genome–transcriptome data from tiger milk mushroom (Lignosus rhinocerus, syn. Lignosus rhinocerotis) to PlantCyc enzymes database identified transcripts that are related to anti-diabetic properties, and these include genes that are involved in carotenoid and abscisic acid biosynthesis as well as genes that code for glyoxalase I, catalase-peroxidases, and superoxide dismutases. The existence of these genes suggests that L. rhinocerus may contain bioactive compound(s) with anti-glycation properties that can be exploited for management of diabetic complications. A medium-molecular-weight (MMW) fraction which was obtained from a combination of cold water extraction and Sephadex® G-50 (fine) gel filtration chromatography of L. rhinocerus sclerotia powder was demonstrated to exhibit potent anti-glycation activity. The fraction specifically inhibited the formation of N𝜀-(carboxymethyl)lysine, pentosidine, and other advanced glycation end-product (AGE) structures in a human serum albumin-glucose system, with an IC50 value of 0.001 mg/ml, almost 520 times lower than that of the positive control, aminoguanidine hydrochloride (IC50 = 0.52 mg/ml). Its ability to suppress protein glycation may be partly associated with its strong superoxide anion radical scavenging activity (10.16 ± 0.12 mmol TE/g). Our results suggest that the MMW fraction of L. rhinocerus shows potential to be developed into a potent glycation inhibitor for preventing AGE-mediated diabetic complications.

Internet pseudoscience: Testing opioid containing formulations with tampering potential

Drug tampering practices, with the aim to increase availability of drug delivery and/or enhance drug effects, are accessible on Internet and are practiced by some portion of recreational drug users. Not rarely, recreational misuse may result in toxic and even fatal results. The aim of the present study was to assess the tampering risk of medicaments containing different formulations of an opioid in combination with paracetamol or dexketoprofen, following the procedures reported in dedicated forums on the web. Tablets and suppositories containing codeine, tramadol and oxycodone were extracted following the reported “Cold water extraction”; dextromethorphan was extracted from cough syrup following the procedure reported as “Acid/base extraction” and fentanyl was extracted from transdermal patches according the procedure reported in Internet. The tampered products and opportunely prepared calibrators in water were analysed by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). The separation of the analytes was carried on Agilent ZORBAX Eclipse Plus C18 (RRHT 2.1 mm × 50 mm, 1.8 μm) by the gradient elution of 0.01% formic acid in water and 0.01% formic acid in methanol. Acquisition was by MRM mode considering at least two transitions for compound. Declared recoveries for these home-made extractions claimed to exceed 99% for the opioid and to complete remove paracetamol, often associated to liver toxicity and thus to obtain a “safer” preparation. In this study, the authors demonstrated that rarely the recoveries for the opioid reached 90% and that up to 60% of the paracetamol amount remained in solution. Thus, high risks for health remained both for the potential lethality of the opioid content, but also for the sub-lethal chronic use of these mixtures, which contained still uncontrolled, ignored, but often important amounts of paracetamol.

Heavy drinkers of Ilex paraguariensis beverages show lower lipid profiles but higher body weight.

Obesity is a widely recognized risk factor for several diseases, reaching an epidemic magnitude worldwide. Natural polyphenols may improve blood lipids and body weight, but their clinical relevance in the general population remains unclear. Thus, we aimed to analyze the relationship of intake of Ilex paraguariensis (I. paraguariensis) beverages to lipid profiles and body weight in a large patient population. Patients were recruited to participate in an educational program to change habits to a healthy lifestyle. Anamnesis, clinical and laboratory assessments were conducted at study enrollment and during follow‐up. I. paraguariensis beverages were defined according to preparation as obtained by repeated cold water extraction (CWE), hot water infusions, or water and sugar decoction. Heavy drinkers were defined as those persons consuming >1 L/day of one or more preparation types. Participants (N = 18,287) aged ≥18 years entered the study. Overall prevalence of I. paraguariensis consumption was 91.2%. All three forms were drunk by 35.7%, whereas CWE + hot water infusion and CWE alone by 28.4% and 14.5% participants, respectively. Heavy CWE drinkers had lower total cholesterol (191.4 ± 49.4 vs. 194.6 ± 48.3 mg/dl, p = .02) and lower low‐density lipoprotein cholesterol (118.6 ± 38.9 vs. 121.2 ± 47.1 mg/dl, p = .001), but body weight was higher (81.1 ± 16.8 vs. 77.2 ± 16.4 kg, p < .0001) compared with moderate drinkers. Fasting glucose was lower (104.5 ± 48.7 vs. 107.2 ± 49.5 mg/dl, p < .001), and consumption of carbohydrates was higher (36.3% vs. 28.7%, p < .001). A low‐lipids high‐body‐weight paradox could be observed in a population of heavy drinkers of I. paraguariensis beverages. Induced hypoglycemia and compensatory higher intake of refined carbohydrates may represent a possible cause.

Evaluation of bioactivity of stem bark extracts of Lovoa trichiliodes (Harm) and Trichilia heudelotii Planc (Harm)

This study evaluated the bioactivity of the stem bark extracts of Lovoa trichiliodes and Trichilia heudelotii using standard methods. The highest yield of 10.20% was obtained from the stem bark extract of T. heudelotii. Qualitative phytochemical examination of the plant extracts indicated the presence of different secondary metabolites which remarkably inhibited the growth of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Salmonella typhii, Aspergillus flavus, Candida albicans and Candida glabrata. However, the cold water extract of L. trichiliodes exhibited no activity against the test pathogens. The highest mean (22.33±0.33 mm) zone of inhibition and minimum inhibitory concentration (MIC) of 2.5 mg/ml were exhibited by the acetone stem bark extract of L trichiliodes against B. subtilis ATCC6633. The results affirmed the traditional uses of the plants in the management and treatment of numerous diseases caused by the test pathogens.

Application of thermal stability difference to remove flammutoxin in fungal immunomodulatory protein, FIP-fve, extract from Flammulina velutipes

Fungal immunomodulatory protein (FIP-fve) is a potential functional food ingredient. However, undesirable component flammutoxin (FTX) would occur in the extracted fraction of FIP-fve. In this paper, an application of heating processing instead of the intensive separation process was employed in fractionation of FIP-fve, meanwhile, exclusion of FTX was reached. Contents of FIP-fve and FTX were monitored by HPLC-UV-ESI-MS. Both FIP-fve and FTX had higher thermal stability in a lower concentration solution. Cold water could effectively extract FIP-fve and FTX from fresh mushroom without acetic acid and disulfide-bond breaking agent β-mercaptoethanol commonly used in biochemical studies. Heating cold water extract contained 580 μg/mL FIP-fve and 452 μg/mL FTX at 60 °C for 5 min could effectively exclude FTX and remain 75% of FIP-fve. Adding 0.1 M trehalose or 20% ethanol did not significantly alter the stability of both proteins. The method developed is an applicable procedure for preparing FIP-fve solution free of FTX.

Determining alkali leaching during accelerated ASR performance testing and in field exposed cubes using cold water extraction (CWE) and µXRF

Considerable leaching of alkali metals was documented, both in concrete samples exposed to accelerated laboratory testing and field samples. CWE allowed to quantitatively determine the free alkali metal profiles as a function of the depth. However, CWE cannot account for the changes in the paste content towards a cast surface leading to a seemingly increase in alkali metals. The μXRF allowed to distinguish paste and aggregates. It allowed thereby to determine qualitative Na and K profiles in the cement paste phase of the concrete samples. The laboratory exposed samples showed a clear leaching profile into a depth of about 15 mm after 21 weeks of exposure at 60°C. Corresponding numbers for the 12 years field exposed cube were 50-60 mm. Alkali sorption by alkali silica gel was detected using the μXRF. For the laboratory exposed samples, the prisms prepared with Portland fly ash cement leached less alkali compared to the prisms prepared with ordinary Portland cement, as expected. The leaching in the middle of the prisms estimated based on the μXRF profiles agreed rather well with the level of alkali leaching determined based on the analysis of the leachate (i.e. the water below the samples during exposure).

In vitro anti-inflammatory activity of Ficus racemosa L. bark using albumin denaturation method.

Introduction:In Ayurveda, many natural plant compounds are used to inhibit inflammatory pathways for centuries with less side effects. Different parts of Ficus racemosa L. (Udumber) plant are used in Ayurveda for many diseases. However, few studies have been conducted to evaluate pharmacological activities of F. racemosa.Objective:The objective of the study was to in vitro analyze anti-inflammatory property of F. racemosa bark using albumin denaturation activity.Methodology:F. racemosa bark extraction was performed using cold water and hot water. The concentration gradient of extracts was prepared using egg albumin and phosphate-buffered saline. The extract was incubated in a water bath at 37°C for 15 min and was heated at 70°C for 5 min. One nonsteroidal anti-inflammatory drug and one steroid were used as reference drugs. The percentage inhibition of protein denaturation was calculated.Results:The inhibition rate of egg albumin denaturation for water extraction increased gradually with concentration. Significantly higher inhibition was showed in hot water extracts than cold water extracts at the concentration of 0.01 μg/ml and 0.1 μg/ml. In addition, the inhibition rate of water extraction was significantly higher than the reference drugs (P < 0.05).Conclusion:Anti-inflammatory activity increases with the concentration of F. racemosa bark. Furthermore, the action of this plant is significantly higher than the reference drugs.

Effect of eucalyptus extracts on the vegetative growth and yield of Hordeum vulgare plant

This study was conducted in AL- Qadisiyah governorate in the agricultural season 2016 - 2017 in order to study the water and alcohol extract of Eucalyptus plant in the growth and yield of Hordeum vulgare plant the local variety. The seeds were sown in the soil and irrigated and after the appearance of four leaves, the plants were sprayed with plant extracts (cold water, hot water, alcohol) with concentrations (0, 0.5, 2, 4, 6)% We have (45) treatment. The results showed that the concentrations used for water and alcohol extracts resulted in an increase in plant height, stem diameter, number of leaves, fresh and dry weight of plant vegetativepart, number of spikes and weight of 100 grains. The results showed that the extraction method has a significant effect on the studied traits. The cold water extraction method resulted in an increase in the dry weight of the plantvegetativepart, The hot water extract method increased both the fresh weight of theplant vegetative part and the weight of 100 grains , Alcohol has caused an increase in plant height, stem diameter and number of leaves.

Preliminary study in improving the malting properties of Dawa, local Sorghum (Sorghum vulgare) variety.

Improving the malting properties of local sorghum [Dawa] (sorghum vulgare) was investigated. Malting is the germination of cereal gain in moist air under controlled conditions, the primary objective being to promote the development of enzymes which are not present in the ingeminated grain. Steeping, germination and kilning temperatures and conditions were altered to affect the improvement of the malt to be produced. Aseptic conditions were employed to avoid contamination of the process. A steeping regime of 52 hours was adopted. The steeping cycle involves 22 hours water steep and 8 hours air rest. Germination was done for 5 days during which enzymes development took place. Kilning was done in an oven for 24 hours at 500C so as to arrest germination. After these, grain and malt analyses were carried out. During the analyses, the following results were obtained: Moisture content (5.4%), thousand corn weight (28.5g), Germinative energy (95.5%), Germinative capacity (90%), cold water extract (44.8%), hot water extract (23%) and malting loss (13%). The result obtained shows that the sorghum variety had high malting loss which was attributed to the high germination temperature used. Also from the result, a Germinative capacity of 90% was gotten. This makes the sorghum (Dawa) variety a good grain raw material for brewing.

곰취 열수 추출물의 항산화 및 항염증 활성

This study was carried out to investigate the anti-oxidative and anti-inflammatory effects of hot water extracts of Ligularia fischeri cultivated in Youngyanggun. We obtained hot water extract (HWE) and cold water extract (CWE) from L. fischeri. The anti-oxidative activities of L. fischeri extracts were measured by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. The anti-inflammatory effects of L. fischeri were evaluated in human mast cell line-1 (HMC-1) cells stimulated with phorbol-12-myristate-13-acetate plus A23187 (PMACI). The solid yields of HWE was 150% higher than CWE solid yield. Total polyphenol contents of HWE were 198.07±0.24 mg/g. The value of anti-oxidative activities of HWE were shown IC50 28.2±0.04 ug/mL. We showed that HWE significantly reduced the PMACI-induced the production of IL-6 (0.01-1 mg/mL), IL-8 (0.1-1 mg/mL), and TNF-α (0.01-1 mg/mL). These results indicate that the HWE of L. fischeri can be used as a functional material due to its antioxidant and anti-inflammatory activities.

Life cycle assessment of tannin extraction from spruce bark

Abstract: Tannins have shown antifungal effects and have been considered a potential natural compound for wood preservation. Extracts produced from softwood bark contain both tannins and non-tannin compounds, which may reduce the effectiveness of tannin used as a wood preservative. The purpose of this research is to study the environmental impact of hot water extraction, identify the hot spots within the tannin cradle-to-gate life cycle and give suggestions to optimize its environmental profile. Different extraction and post-extraction scenarios of tannin production are compared using the life-cycle assessment method. Experiments were designed to study the tannin yield under different extraction scenarios; the post-extraction scenario analysis was based on literature review. The results show that the extract drying process is the primary contributor to the environmental impact of tannin production. Both preliminary cold water extraction and ultrafiltration after extraction are beneficial as they have fewer non-tannin compounds in the final products; however, preliminary cold water extraction had a considerably lower environmental performance. Successive extractions using fresh water at each cycle increased the total tannin yield, but increased the environmental burden. Using only evaporation to obtain a desired tannin concentration is not environmentally efficient. This paper provides a quantified environmental analysis for the development of tannin-treated wood products and discusses the different tannin extraction scenarios from an environmental point of view.

Molecular Weight Affected Antioxidant, Hypoglycemic and Hypotensive Activities of Cold Water Extract from Pleurotus citrinopileatus.

Cold water extract of P. citrinopileatus (CWEPC) was fractioned into 4 fractions, PC-I (<1 kDa), PC-II (1-3.5 kDa), PC-III (3.5-10 kDa), and PC-IV (>10 kDa), by ultrafiltration. The antioxidant activities, the inhibition of pancreatic α-amylase, intestinal α-glucosidase, and hypertension-linked angiotensin converting enzyme (ACE), as well as the contents of polysaccharides, protein, and phenolic compounds of 4 fractions were determined. The results showed that lower MW fractions exerted a higher antioxidant activity, which was correlated to phenolic contents. The high molecular fraction (PC-IV) exhibited significantly higher inhibitory activity on α-amylase, α-glucosidase, and ACE compared to CWEPC and the other 3 lower MW fractions (<10 kDa), which was more related to protein contents. The inhibition capability of CWEPC and PC-IV on α-amylase activity was 1/13.4 to 1/2.7 relative to that of acarbose, respectively. Kinetic data revealed that PC-IV fraction followed a noncompetitive inhibition pattern on α-glucosidase activity. The study demonstrated that various MW fractions and types of components contribute to different biological functions of P. citrinopileatus and it is protein constituents but not peptides responsible for the hypoglycemic potential of CWEPC.

The effect of different extraction techniques on property and bioactivity of polysaccharides from Dioscorea hemsleyi

The rhizoma of Dioscorea hemsleyi (DH) has been used as a treatment of diabetes in China for hundreds of years. Polysaccharides in DH were extracted by using ultrasonic-assisted extraction (UAE), cold water extraction (CWE), warm water extraction (WWE) and hot water extraction (HWE), separately. Then the different characterizations of four DH polysaccharide (DHP) samples were analyzed by high-performance liquid chromatography (HPLC), high-performance Gel permeation chromatography (HGPC), ultraviolet-visible spectroscopy(UV), fourier transform-infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Their activities in vitro of DHP were compared. Experimental results showed that HWE had the highest yield and large molecular weight. CWE had the highest uronic acid yield and little molecular weight, and its DPPH, AGI and AAI activity were the best. The molecular weight of UAE was small, and its RP and FRAP activity were the best. Four DHP samples had differences in the surface topography, while they all had the typical IR spectra characteristic of polysaccharides. According the correlation analysis, it showed that the more uronic acid and the lower molecular weight was, the higher the antioxidant activity was. The high content of monosaccharide composition of Xyl, Ara, GlcA and GalA, and little molecular weight have good effect on antidiabetic activity.

Phenolic linked anti-hyperglycemic bioactives of barley (Hordeum vulgare L.) cultivars as nutraceuticals targeting type 2 diabetes

Barley (Hordeum vulgare L.) is an important commercial and industrial crop rich in phenolic bioactives and dietary antioxidants and can be targeted for developing non-food nutraceuticals and functional ingredients to counteract diet-linked chronic diseases such as type 2 diabetes (T2D). In this study 13 barley cultivars were screened for phenolic-linked functionalities targeting antioxidant and anti-hyperglycemic properties using in vitro assay models. Total soluble phenolics (TSP) content, individual phenolic acid profile, and antioxidant activity of barley extracts (hot water, cold water, and 12% ethanol) were evaluated. The α-amylase and α-glucosidase inhibitory activities of barley extracts were also evaluated using in vitro assays. The TSP content of barley varied between 0.44 and 0.63mg/g gallic acid equivalent (GAE) on a dry weight basis across cultivars and extraction types, with hot water extracts having highest TSP content. Across barley cultivars, cv. Pinnacle had the highest antioxidant activity, while among extraction types ethanol extracts had highest antioxidant activity. Cold water and ethanol extracts of most barley cultivars had significantly high α-amylase inhibitory activity; however, significant differences were not observed among most cultivars. The black barley cultivar had the highest α-glucosidase inhibitory activity (34%) across all extraction types, and a dose-dependent pattern was observed in all barley cultivars. Therefore, barley cultivars with significant potential to modulate the activity of key enzymes that regulate carbohydrate metabolism were screened and identified in this in vitro study. Consequently, grains of such cultivars may be utilized for further clinical and animal studies to develop phenolic bioactive-rich non-food type nutraceuticals and bioactive functional ingredients that can be used to complement current drug design strategies to offset hyperglycemia and oxidative stress associated with early stages of T2D.

Thermal decomposition of pyrolytic lignin under inert conditions at low temperatures

A pyrolytic lignin, separated from the bio-oil generated from biomass fast pyrolysis bio-oil at 500°C via cold-water extraction, was thermally decomposed in a drop-tube/fixed-bed reactor under inert conditions at 100–350°C. It is found that the char yield of the pyrolytic lignin under such fast pyrolysis conditions decreases rapidly from ∼82% at 100°C to ∼23% at 350°C. The majority of the weight loss is contributed by the significant reduction in the fraction of light aromatic oligomers (i.e., the CH2Cl2-soluble fraction) retained in the pyrolytic lignin chars, with such reductions increasing with temperature. On the contrary, there is insignificant change in the fraction of heavy aromatic oligomers (i.e., the CH2Cl2-insoluble fraction) retained in the pyrolytic lignin chars during pyrolysis at such low temperatures. It is evident that there are strong interactions between the light and heavy aromatic oligomers during thermal decomposition of the pyrolytic lignin, leading to the observed additional char formation. The main reactions during thermal decomposition of the pyrolytic lignin are the release of aliphatic and small phenolic compounds (i.e., via demethylation and decarbonylation reactions), mainly due to the breaking of ether bonds in the pyrolytic lignin structure. The char structure becomes more aromatic as temperature increases, due to the enhanced polymerization reactions at high temperatures (>250°C).

Study the Effect of Cold and Hot Water Extracts of Parsley Plant Petroselinumcrispum on the Growth of some Enterobacteriaceae

This study was conducted to determine the effect of hot and cold water extractsof parsley leaves on five genus of bacteria belong toenterobacteriaceae familynomely; E.coli, Enterobacter, Klebsiella, Shigellaand Salmonella after 24 or 48 hrs of incubation where the number of growth colonies on macConkey agar appears. The efficiency of these extractsto prevent bacteria from growthwas indicated by using the percentage ofdilution value. The resultsshowed that the hot water extract was more efficientin most treated bacteria compared with cold water extract. Also resultsindicated that both Salmonella andEnterobacterwere more sensitive toward both extracts incomparsion with other studied bacteria, such asKlebsiellashowed higher resistance toward both extracts. Additionally, significant differencesbetween number of colonies grown on MacConkeymedium that treated with hot or cold water extracts were recorded.

Biological effect of Lawsonia inermis plant

This study was compared the antimicrobial effectiveness of Lawsonia inermis plant extract (alcoholic, aqueous and silver nanoparticles) on the growth of different types of Gve+ bacteria and Gve- bacteria at concentration 80 mg /L. It was noted that the ( alcoholic, hot and cold water) extract have highest impact recorded in the growth of bacteria S.aurous with inhibition zone (26mm) followed by the bacteria P.aueroginosa at inhibition zone reached (22mm) and finally the yeast C. albicans at inhibition zone reached (17mm), while silver nano-particles of the plant extract the most influence on the bacteria, it gave the highest Inhibition area reached (30mm) on the growth of the S. aurous, followed by the inhibition area of P.aueroginosa reached (26mm) then Strep. pyogein (25mm), and finally the yeast C. albicans reached (18 mm). With regard to the complex (alcoholic extract and antibiotic Amoxylein) its effects has varied upon microorganisms, there is a synergistic effect on the growth of the yeast C. albicans at inhibition zone (30mm) while it has been observed inhibitory effect of this complex on the growth of p. aeroginosa with inhibition zone amounted to (15mm), but did not show any significant impact on the growth of both bacteria (K. pneumonia, E.coli Strep. Pyogein&amp; P. aeroginosa,), while the (alcoholic extract and antibiotic Erythromycin) showed inhibitory and synergistically effect on each of the bacteria (E.coli, S. aurous, yeast C. albicans and P. aeroginosa) respectively. According to obtained results the antibiotic Erythromycin shows higher inhibitory effect than antibiotic Amoxylein. It has also been investigated the lower values of absorbance at the wavelength of 260 nm with increasing the concentration of crude alcoholic extract, this reveled the ability of henna extract in the removal of free radicals liberated from photolysis of hydrogen peroxide compound .