Cold Temperature Regimes Research Articles

Thermal stability analysis of particles incorporated in cirrus crystals and of non-activated particles in between the cirrus crystals: comparing clean and polluted air masses

Abstract. A thermal volatility technique is used to provide indirect information about the chemical composition of the aerosol involved in cirrus cloud formation. The fraction of particles that disappears after being heated to 125°C is termed volatile and the fraction that disappears between 125 and 250°C is termed semi-volatile. Particles that still remain after being heated to 250°C make up the non-volatile fraction. The thermal composition of residual particles remaining from evaporated cirrus crystals is presented and compared to interstitial aerosol particles (non-activated particles in between the cirrus crystals) for two temperature regimes (cold: T< -38°C, warm: -38≤T< -23°C), based on in-situ observations. The observations were conducted in cirrus clouds in the Southern Hemisphere (SH) and Northern Hemisphere (NH) midlatitudes during the INCA project. In the cold temperature regime, the non-volatile fraction of the residual particles was typically in the range 10 to 30% in the NH and 30 to 40% in the SH. In the warm temperature regime, the non-volatile residual fraction was typically 10 to 30% (NH) and 20 to 40% (SH). At high crystal number densities the non-volatile fraction in both temperature regimes was even higher: in the range of 30 to 40% (NH) and 40 to 50% (SH). The semi-volatile fraction was typically less than 10% in both hemispheres, causing the volatile fraction to essentially be a complement to the non-volatile fraction. In terms of the fractioning into the three types of particles, the SH cold case is clearly different compared to the other three cases (the two warm cases and the cold NH case), which share many features. In the NH data the distribution of different particle types does not seem to be temperature dependent. In all the cases, the non-volatile fraction is enriched in the residual particles compared to the fractions observed for the interstitial particles. This enrichment corresponds to about 15 (NH) and 30 (SH) percent units in the two cold cases and to 15-25 (NH) and 25-35 (SH) percent units in the two warm cases. In the NH cold case, there is a clear relation between the fractions observed in the interstitial particles and what is observed in the residual particles. The observed large fractions of non-volatile particles show that particles forming ice crystals are not entirely made up of water-soluble sulfate particles.

Controls on methane production in a tidal freshwater estuary and a peatland: methane production via acetate fermentation and CO2 reduction

Rates of total methane production, acetate fermentation and CO2 reduction were compared for two different wetland sites. On a per-liter basis, sediments from the White Oak River estuary, a tidal freshwater site in eastern North Carolina, had an annual methane production rate (53.3 mM yr-') an order of magnitude higher than that of Buck Hollow Bog (5.5 mM yr-'), a peatland in Michigan. Meth- ane was produced in the White Oak River site on an annual basis by both acetate fermentation (72%) and CO2 reduction (28%) in a ratio typical of freshwater methanogenic sites. Competition for acetate by non-methanogenic microorganisms in Buck Hollow peat limited methane production from acetate to only a few months a year, severely impacting annual methane production rates. However, when acetate was available to the methanogens in the peat during early spring, the percentage of methane production from acetate fermentation (84%) and CO2 reduction (16%) and rates of total methane production were similar to those of the White Oak River sediments at the same temperature. Rates of CO2 reduction and acetate fermentation conducted at both sites at various temperatures showed that Buck Hollow peat methane production was also limited by a colder temperature regime as well as differences in the re- sponse of the CO2 reducing and aceticlastic methanogens to temperature variations.

Regulation of frost resistance during cold de-acclimation and re-acclimation in oilseed rape. A possible role of PSII redox state.

A possible role of photosynthetic apparatus during cold de-acclimation was studied in oilseed rape (Brassica napus var. oleifera). Plants of spring (Star) and winter (Górczañski) cultivars were cold acclimated at + 5 degrees C, and de-acclimated during 4 weeks at combinations of + 12 and + 20 degrees C operating in the light or/and dark, with a 12-h photoperiod. Evidence is presented that the photosynthetic apparatus may be involved in temperature perception during de-acclimation. De-acclimation was faster under a 20/12 degrees C (day/night) treatment than under the reverse 12/20 degrees C (day/night). De-acclimation rate was constant when the day temperature was constant, irrespective of the night temperature both under cold day temperature regimes (12/20, 12/12 degrees C (day/night) and warm-day treatments (20/12, 20/20 degrees C (day/night). The fast decrease in frost resistance observed under warm-day de-acclimation was always accompanied by an acceleration of elongation growth. In the spring cultivar, elongation growth increased starting from the second week of de-acclimation, regardless of temperature conditions. Once elongation growth had commenced during de-acclimation, it continued throughout the period necessary for re-acclimation to low temperature. Re-acclimation to the initial freezing tolerance level was only possible when plant elongation was reduced. In addition re-acclimation of the photosynthetic apparatus to low temperature was impossible in fast growing plants. A possible relationship between PSII, growth rate and frost resistance during cold acclimation and de-acclimation is discussed.

Temperature influence on Arctic charr ( Salvelinus alpinus L.) antibody response to a cellular antigen

In order to elucidate the immune responses of Arctic charr in relation to temperature, groups were acclimated to a moderate (9°C) and a cold temperature regime (4°C), as well as subjected to a temperature decrease (from 9 to 4°C) immediately prior to an immunization with sheep red blood cells. The charr kept at 9°C responded with increased primary and secondary antibody titres, as seen by direct haemagglutination, while fish at 4°C, as well as the fish subjected to a temperature reduction, displayed lower and lowest antibody titres, respectively, and only after a second immunization. It is concluded that Arctic charr can respond to a cellular antigen with a humoral immune response typical for other teleosts, but that the immune response is delayed and diminished at low temperatures. This temperature-induced immune suppression is intensified if the fish have not been acclimated to cold water prior to immunization.

Close-coupled calculation of collisions of magnetostatically trapped metastable helium atoms

A quantum-mechanical close-coupled calculation for collisions of spin-polarized triplet helium atoms in a magnetostatic trap is presented in which the Penning and associative ionization processes are represented by a complex optical potential. The rate coefficients for the spin relaxation and relaxation-induced Penning ionization processes for a range of magnetic fields and temperatures, spanning the cold and ultracold temperature regimes, have been calculated and the sensitivity of these results to uncertainties in the molecular potentials and autoionization width investigated. The rate constants for both elastic and inelastic processes were found to be particularly sensitive to the short-range ${}^{5}{\ensuremath{\Sigma}}_{g}^{+}$ molecular potential and, to a lesser extent, the rate constants for relaxation-induced Penning ionization displayed sensitivity to the short-range form of the ${}^{1}{\ensuremath{\Sigma}}_{g}^{+}$ molecular potential and the autoionization width.

Latitudinal compensation in oyster ciliary activity

1. Theories of latitudinal compensation predict that individuals living in colder temperature regimes should physiologically compensate for the slowing of standard physiological rates, owing to the relatively low temperature of their local environments, by increasing their metabolic rate in colder water temperatures relative to individuals living in warmer water temperature regimes. 2. This hypothesis was tested with oyster strains originally from geographically separated populations that were raised in a common environment for seven generations. The physiological parameter measured was ciliary activity across a temperature gradient. 3. Support for the latitudinal compensation hypothesis was found: the strain originally from the colder temperature regime had more active cilia at lower experimental temperatures than individuals originally from the warmer temperature regime. Ciliary activity of the more northern Long Island Sound oysters was significantly greater than activity in the more southern Delaware Bay oysters at temperatures of –1, 2 and 6 °C. 4. These results suggest that there is genetically based physiological differentiation between these populations of oysters consistent with the latitudinal compensation for local temperature regime.

Geographic distribution and seasonal variation of mitochondrial DNA haplotypes in the aphidRhopalosiphum padi(Hemiptera: Aphididae)

AbstractThis study examines the spatial and seasonal patterning of mitochondrial DNA diversity in French populations of the bird cherry-oat aphid,Rhopalosiphum padi(Linnaeus), on both its primary and secondary hosts. Our results confirm the presence of two major mitochondrial lineages that are generally associated with the breeding system variation (cyclic and obligate parthenogenesis) shown by this species. The strength of this relationship varies regionally, being most evident in the south and west. Cyclically parthenogenetic populations show no significant regional or seasonal genetic divergence reflecting high levels of gene flow, possibly promoted by their obligate host-alternation. However, obligately parthenogenetic populations show a north-south cline in the distribution of the dominant haplotypes. This pattern might result from a selective advantage of some obligately parthenogenetic lineages under cold temperature regime. Alternatively, this cline might be established by a gradient in the intensity of nuclear gene flow between cyclically and obligately parthenogenetic populations mediated by androcyclic males. The discrimination between these possible explanations will require extending analysis to include hypervariable nuclear markers.

Influence of Temperature on Germination of Japanese Brome Seed

Japanese brome (Bromus japonicus Thunb.), an introduced annual grass, is now common in some northern mixed-prairie communities. This species has the potential to alter both the seasonality of standing crop and forage quality. We sought to gain a greater understanding of Japanese brome seed germination by subjecting seed to a series of 55 constant or alternating temperature regimes following 3 to 9 months of dry laboratory storage. Cold and moderate temperature regimes provided optimum germination conditions (defined as not lower than the maximum observed minus one-half its confidence interval at the 0.05 level of probability). Extremely cold or warm temperatures suppressed germination. Germination of afterripened seed over a wide range of temperature combinations, many of which occur during fall in the Northern Great Plains, should enhance establishment and perpetuation of Japanese brome on rangelands.

The thermal biology of the white-tailed rat Mystromys albicaudatus, a cricetine relic in southern temperate African grassland

This study examines the hypothesis that Mystromys albicaudatus, a cricetine relic in southern Africa, has thermal characteristics typical of a rodent adapted to a cold temperature regime. Metabolic rate (oxygen consumption) of M. albicaudatus was measured using open-flow respirometry at ambient temperatures ranging from 5 degrees C to 35 degrees C. Lowest specific oxygen consumption was 1.352 +/- 0.089 ml O2 g-1hr-1 (n = 8; body mass = 93.78 +/- 6.27 g) at 25 degrees C, equivalent to 121.8% of the predicted value of Kleiber (1975), 128.8% of the value predicted for eutherians and 113.7% of the value predicted for cricetidae (Hayssen and Lacy, 1985).

INFLUENCE DE TRAITEMENTS PRÉALABLES AU FROID SUR LA CROISSANCE ET LE DÉVELOPPEMENT DU POINSETTIA

To achieve more rapid adaptation of plants grown under a constant cold night temperature regime, rooted poinsettia (Euphorbia pulcherrima Willd ex. Klotzsch ’Annette Hegg Dark Red’) cuttings were given one of several cold pretreatments (TPF): either 17/13 °C or 13 °C for 5, 10, or 15 d. The influence of these TPF treatments was compared with that of control plants where the night temperature was 17 °C. There was no significant difference for the number of days to the first visible flower buds and to anthesis, for the number of flowering stems and bracts formed, for leaf area and aerial dry weight. However, the TPF treatment of 17/13 °C given for 15 d caused a significant increase in bract area, and of average flower head diameter compared to those plants given a TPF treatment of 13 °C for the same length of time.Key words: Euphorbia pulcherrima, night temperature, split night temperature, pretreatment

Radio Echo-Sounding of Sub-Polar Glaciers in Svalbard: Some Problems and Results of Soviet Studies

The paper discusses data analysed from airborne radio echo-sounding of Svalbard glaciers at frequencies of 440 and 620 MHz. Bottom returns from depths greater than 200 m are recorded with fewer gaps if the more powerful 620 MHz radar is used, and if measurements are carried out in the spring before intensive melt on glaciers. For all relatively thin glaciers and some glaciers up to 320–625 in thick, the track with bed returns is still rather common, apparently caused by their colder temperature regime. However, because of severe scattering of radio waves, this procedure still does not solve the problems of the echo-sounding of accumulation areas of many of the larger glaciers, the ice plateau, and heavily crevassed parts of glaciers.For considerable areas of those Spitsbergen glaciers which have a thickness greater than 200 m, internal radar reflections (IRR) were registered as a single isolated layer from depths usually ranging from ¼ to ½ of their thickness. Studies of two deep bore holes on Fridtjovbreen have- demonstrated that such IRR are related to a boundary between cold ice and water-bearing ice near the melting point. These IRR can be interpreted as indicators of a special class of two-layered or transitional glacier, and of the location within them of the ice-melt isotherm.

Radio Echo-Sounding of Sub-Polar Glaciers in Svalbard: Some Problems and Results of Soviet Studies

The paper discusses data analysed from airborne radio echo-sounding of Svalbard glaciers at frequencies of 440 and 620 MHz. Bottom returns from depths greater than 200 m are recorded with fewer gaps if the more powerful 620 MHz radar is used, and if measurements are carried out in the spring before intensive melt on glaciers. For all relatively thin glaciers and some glaciers up to 320–625 in thick, the track with bed returns is still rather common, apparently caused by their colder temperature regime. However, because of severe scattering of radio waves, this procedure still does not solve the problems of the echo-sounding of accumulation areas of many of the larger glaciers, the ice plateau, and heavily crevassed parts of glaciers. For considerable areas of those Spitsbergen glaciers which have a thickness greater than 200 m, internal radar reflections (IRR) were registered as a single isolated layer from depths usually ranging from ¼ to ½ of their thickness. Studies of two deep bore holes on Fridtjovbreen have- demonstrated that such IRR are related to a boundary between cold ice and water-bearing ice near the melting point. These IRR can be interpreted as indicators of a special class of two-layered or transitional glacier, and of the location within them of the ice-melt isotherm.

Alternative Respiratory Path Capacity in Plant Mitochondria: Effect of Growth Temperature, the Electrochemical Gradient, and Assay pH

Influence of growth temperature on the capacity of the mitochondrial alternative pathway of electron transport was investigated using etiolated corn (Zea mays L.) seedlings. These seedlings were grown to comparable size in either a warm (30 degrees C) or a cold (13 degrees C) temperature regime, and then their respiration rates were measured as O(2) uptake at 25 degrees C. The capacity of the alternative pathway (KCN-insensitive O(2) uptake) was found essentially to double in shoots of cold-grown seedlings. This increased capacity slowly developed over several days growth in the cold, but was lost within 1 day when the seedlings were exposed to a warm regime. When mitochondria were isolated from the shoots of these seedlings, a greater potential for flow through the alternative path was observed in mitochondria from the cold-grown seedlings with all substrates used (an average increase of 84%). Using exogenous NADH as the substrate, the effect of the electrochemical gradient on measurable capacities of the cytochrome and alternative pathways was investigated in mitochondria from both etiolated seedlings and thermogenic spadices. The uncoupler FCCP (p-trifluoromethoxycarbonylcyanide phenylhydrazone) was used to diminish the electrochemical gradient when desired. In corn (Zea mays L.) shoot and mung bean (Vigna radiata L.) hypocotyl mitochondria, which have relatively low capacities of the alternative pathway, increased flow through the cytochrome chain in the absence of the electrochemical gradient was found not to influence the potential for flow through the alternative path. However, in mitochondria from skunk cabbage (Symplocarpus foetidus L.) and voodoo lily (Sauromatum guttatum Schott) spadices, which have high capacities of the alternative pathway, increased flow through the cytochrome chain in the absence of the gradient occurred at the expense of flow through the alternative pathway. These results suggest that in mitochondria of thermogenic spadices, the combined capacities of the cytochrome and alternative paths exceed the capacity of the exogenous NADH dehydrogenase. The effect of assay pH on measurable capacities of the cytochrome and alternative paths was determined over a pH range of 5.6 to 8.8 using exogenous NADH as the mitochondrial substrate. When the electrochemical gradient was present, it limited the electron transport rate and little effect of assay pH was observed. However, when formation of the gradient was prevented through inclusion of FCCP, measurable capacities of the cytochrome and alternative paths were found to be greatly influenced by pH. This experiment also revealed that the potential for respiratory control is largely dependent upon the assay pH.

COMPARISON OF CANADIAN AND AMERICAN CLASSIFICATION SYSTEMS FOR SOME ARCTIC SOILS OF THE UNGAVA-LABRADOR PENINSULA

Thirteen pedons in an arctic environment were classified according to the Canadian and American systems of soil classification. Major differences in groupings result from the contrasting approaches. Although the definition used in Canada to define Cryosols is sometimes difficult to apply in the field, the underlying concept is valid, since soils with permafrost at a shallow depth should be separated at the highest level of classification. The American system virtually ignores the influence of permafrost on pedogenesis. Its reliance on soil temperature regimes at both the great group and subgroup levels is redundant. It is suggested that the definition of Cryosols, in the Canadian system, be changed to include strongly cryoturbated soils with very cold or colder soil temperature regimes. The American system should be altered to provide for pergelic great groups; in addition permafrost and strong cryoturbation should be used to create new subgroups. The requirement that a cambic horizon have a texture finer than loamy fine sand should be waived in soils having cryic or pergelic soil temperature regimes. Key words: Cryosols, cryoturbated soils, permafrost, pergelic soils

Cold Tolerance Evaluation of Several Centipedegrass Selections1

AbstractCentipedegrass (Eremochloa ophiuroides (Munro) Hack.), an excellent lawn grass throughout much of the lower South, is often limited by its lack of cold tolerance. Studies were conducted to determine variability in cold tolerance among centipedegrass selections as affected by hardening conditions. Seven clonal selections were grown under controlled environmental conditions using sand culture and cold hardened during a 21‐day exposure with a 12‐hour photoperiod, 22 C day (16 C day in the second experiment), 4 C night. Unhardened plants recieved a 31 C day, 25 C night.In one experiment, following hardening treatments, plants were exposed to —9.7 C for 1.5, 3, 5, 7 or 9 hours. In a second experiment plants were exposed to —3.0, —5.6, —7.3, or —10.1 C for 4 hours. After low‐temperature exposure plants were transferred to favorable growing conditions and rated for top‐kill and survival after 7 and 21 days respectively.Top‐kill increased and survival decreased as duration of cold exposure increased or temperature decreased. Exposing centipedegrass to cold hardening temperature regimes prior to freezing resulted in less top‐kill and more survival. Cold hardening increased low‐temperature tolerance a maximum of 2.3 C; i.e., percent survival was the same for hardened plants at —9.6 C as for unhardened plants at —7.3 C. Selections differed in amounts of top‐kill. Mississippi I, Tennessee Hardy, Oklawn, and FC‐2 were generally less susceptible to top‐kill than PllA,, Mississippi II, and FC‐8. No survival differences were noted among Centipedegrass selections following low‐temperature exposures.