This presentation will discuss various approaches combining light and sound for tissue characterization. All the approaches discussed here involve the optical detection of motion generated at depth in tissue by use of a focused ultrasound transducer. In the first part of the presentation, the acousto-optic imaging approach will be described, in which the optical phase modulation created by an ultrasound beam is used to derive optical properties with the resolution of the ultrasound beam. In particular, it will be demonstrated that randomly phase-modulated millisecond ultrasound burst can be used to obtain both transverse and axial resolutions. In this first approach, the optically detected motion consists of the ultrasonic motion of insonified light scatterers. In the second part of the presentation, it will be demonstrated that it is also possible to optically detect the transient shear motion generated by the acoustic radiation force in soft tissue. As for acousto-optic imaging, this approach is based on the interaction between a diffuse coherent light field and a localized motion and therefore provides localized information in the sample. In addition to provide information on optical properties, this approach also provides information on shear stiffness on the medium. For both approaches, experimental results obtained in vitro on tissue or tissue-mimicking phantoms will be shown, and the current challenges toward in vivo measurements will be discussed.
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