Coefficient Of Variation Range Research Articles

Validity and reliability of the T-Force and Chronojump systems to measure movement velocity on resistance machines with older adults

The current study aimed to analyze the validity and reliability of the T-Force and Chronojump systems to measure the movement velocity in the leg press (LP) and chest press (CP) exercises in older people. Eighteen older adults (6 men and 12 women, 79.9 ± 8.5 years) performed a set of procedures over three weeks: (i) the first week was to familiarize participants with the testing procedures, (ii) the second was to perform a progressive loading test until reaching one-repetition maximum (1RM) in the LP and CP, and (iii) in the third week, participants performed three repetitions against five loads (40, 50, 60, 70, and 80% of 1RM). The mean velocity of each repetition was recorded simultaneously through the T-Force and Chronojump devices. Linear regressions (coefficient of determination [r2] and standard error of the estimate [SEE]) analyzed the inter-device validity, and Bland-Altman plots illustrated the systematic differences between devices. A mixed-effects model estimated the mean velocity differences between devices. The relative reliability was analyzed by the intra-class correlation coefficient (ICC[1,k]), while the absolute reliability was by the standard error of measurement (SEM) and the coefficient of variation (CV). The results showed that the T-Force and Chronojump presented a high association level in measuring mean velocity in the LP and CP (r2 range: 0.96–0.99; SEE range: 0.01–0.02 m·s− 1) and low systematic bias (0.02–0.03 m·s− 1). The mean velocity values of T-Force were significantly higher than Chronojump only for 40% 1RM (p = 0.04). Excellent reliability inter-device (ICC range: 0.95–0.98; CV range: 1.7–3.2%) and intra-device (ICC range: 0.90–0.97; CV range: 3.4–6.5%) was observed. This study shows that the T-Force and Chronojump systems are valid and reliable for measuring movement velocity in the CP and LP machines when used by older adults.

Sleep Duration Irregularity is Associated with Elevated Blood Pressure During Submaximal Exercise in Young Adults.

Irregularity in nightly sleep duration is reported to associate with elevated blood pressure (BP), but it is unclear whether this association can be observed with BP measured during exercise after controlling for factors known to influence the exercise pressor reflex. Twenty-nine young adults (22±4y; 19 men, 10 women) performed cycling exercise until volitional fatigue to assess peak oxygen uptake (VO2). Actigraphy was used to monitor sleep duration and daily physical activity for seven consecutive days after which participants completed two bouts of moderate-intensity cycling while BP and VO2 were measured using a Tango+ device and indirect calorimetry, respectively. Systolic BP was averaged from the two bouts of exercise and expressed as a change from seated rest (∆SBP). Sleep duration regularity was calculated as standard deviation (SD) and coefficient of variation (CV). Systolic BP at seated rest, during exercise, and ∆SBP was 113±13, 152±21, and 38±13 mmHg, respectively. Sleep duration SD (range 10-146 min) and sleep duration CV (range 2-54%) when excluding weekend nights were significantly correlated with ∆SBP (r = 0.58 and r = 0.62, respectively; both p<0.01) after adjusting for age, sex, body mass index, peak VO2, physical activity, resting systolic BP, chronotype, and the VO2 response to exercise. Sleep duration regularity analyzed with weekend nights included (across all seven days) was also significantly correlated with ∆SBP (p≤0.01), but had weaker correlation coefficients. These results indicate that sleep regularity, especially when excluding weekend nights, is associated with the rise in systolic BP during moderate-intensity exercise in young adults. Sleep duration regularity may be a useful tool to capture the impact of intermittent nights of insufficient sleep on BP dysregulation.

Influence of sex on the reliability of cerebral blood velocity regulation during lower body negative pressure and supine cycling with considerations of the menstrual cycle

Objective. To evaluate sex differences in the reliability of absolute and relative cerebral blood velocity (CBv) during concurrent supine cycling with lower body negative pressure (LBNP). Approach. A total of 19 participants (11 females; aged 20–33 years) completed five testing sessions, occurring on 7 d intervals. Visit 1 was a maximal-ramp-cycle test to ascertain peak CBv wattage. During visits 2–5, supine cycling protocol occurred at individualized peak CBv wattages with progressive decreases in LBNP from 0 to −20, −40, −60, −70, and −80 Torr. Menstrual cycle day was self-reported via the Rhinessa Women’s Questionnaire. Transcranial Doppler ultrasound insonated bilateral middle cerebral artery velocity (MCAv). Two-way ANOVA assessed potential day- and sex-differences at each LBNP stage. Reliability was determined using intraclass correlation coefficients (ICC) and coefficient of variation (CoV). Main results. For all physiological measures, no main-effects were present for day- or interaction-terms (p > 0.067; negligible-to-small effect sizes), while sex differences were noted for MCAv, blood pressure, and heart rate (p < 0.046). Across visits, males and females displayed excellent and good-to-excellent levels of reliability for MCAv metrics, respectively (ICC range: 0.745–0.999; CoV range: 0.33%–9.90%). Significance. During the current investigation, both relative and absolute CBv demonstrated high reliability in both male and female participants during a supine LBNP cycling protocol. An exploratory analysis revealed increased variance was found in female participants dependent on contraceptive use. Despite this, results indicate future LBNP studies may include females at any menstrual cycle stage.

Variability of blood pressure and risk of postoperative acute kidney injury in patients undergoing surgery for acute aortic dissection: A 11-year single-center study.

In patients receiving surgery, increased blood pressure variability may contribute to the onset of acute kidney injury (AKI). However, the extent and significance of blood pressure variability in patients undergoing aortic dissection surgery have yet to be determined. The authors analyzed all patients operated for aortic dissection at Peking University Shenzhen Hospital from January 1, 2011 to December 31, 2021. Preoperative blood pressure variability was expressed as standard deviation, coefficient of variation (CV), and range. The primary outcome was postoperative AKI. After multivariate analysis, systolic blood pressure variability was found to be independently associated with AKI after aortic dissection surgery. The odds ratios and 95% confidence intervals for standard deviation, CV, and systolic blood pressure range were 1.232 (1.011-1.500), 1.451 (1.058-1.991), and 1.138 (0.986-1.288) for postoperative AKI, respectively. However, there was no significant relationship between diastolic blood pressure variability and AKI after aortic dissection surgery. Finally, in patients undergoing surgery for aortic dissection, the variability of systolic blood pressure is actually an important factor in the development of AKI.

Classification to Coefficient of Variation in Physical and Chemical Attributes of Oranges

Abstract: A of the ways to evaluate data homogeneity in experiments is through the coefficient of variation (CV), however, in orange fruit evaluations, gaps are noted about the ranges that the variables must present due to their fluctuations as a function of factors genetic, climatic and agricultural. Thus, the present work aims to propose classifications of coefficient of variation for physicochemical attributes of orange fruits. For this purpose, CVs of 42 articles were used, considering soluble solids (SS), titratable acidity (TA), soluble solids and titratable acidity ratio (SS/TA), fruit length (FL), fruit diameter (FD), fruit mass (FM) and juice yield (JY), applying descriptive statistics and the Shapiro Wilk normality test. The limits proposed as low CV for the physical-chemical attributes of total soluble solids, titratable acidity, the ratio of total soluble solids and titratable acidity, fruit length, fruit diameter, fruit mass, and juice yield are respectively 2.11; 4.39; 4.46; 2.27; 2.59; 4.71 and 1.99% while the high CV range corresponds in the same sequence to 9.61;15.02;15.12;8.99;9.69;19.39;16.34%.

Immunological heterogeneity informs estimation of the durability of vaccine protection.

Deciphering the properties of vaccines against an emerging pathogen is essential for optimizing immunization strategies. Early after vaccine roll-out, however, uncertainties about vaccine immunity raise the question of how much time is needed to estimate these properties, particularly the durability of vaccine protection. Here we designed a simulation study, based on a generic transmission model of vaccination, to simulate the impact of a breadth of vaccines with different mean (range: 10 months–2 years) and variability (coefficient of variation range: 50–100%) of the duration of protection. Focusing on the dynamics of SARS-CoV-2 in the year after start of mass immunization in Germany as a case study, we then assessed how confidently the duration of protection could be estimated under a range of epidemiological scenarios. We found that lower mean and higher heterogeneity facilitated estimation of the duration of vaccine protection. Across the vaccines tested, rapid waning and high heterogeneity permitted complete identification of the duration of protection; by contrast, slow waning and low heterogeneity allowed only estimation of the fraction of vaccinees with rapid loss of immunity. These findings suggest that limited epidemiological data can inform the duration of vaccine immunity. More generally, they highlight the need to carefully consider immunological heterogeneity when designing transmission models to evaluate vaccines.

The inter-device reliability of global navigation satellite systems during team sport movement across multiple days

Objectives(1) Determine the inter-device and inter-manufacturer reliability; and (2) investigate the variation in reliability over time for common global navigation satellite systems. DesignRepeated measures. MethodsA total of twenty 10-Hz devices manufactured by StatSports (n = 10, Apex Pro; StatSports, Newry, Ireland) and Catapult Sports (n = 10, Vector S7; Catapult Sports, Melbourne, Australia) were towed on a sprint sled during 8 × 40-minute team sport movement protocol over a 4-week period. The coefficient of variations for distance, velocity and acceleration/deceleration metrics were calculated to show dispersion of the data relative to the mean or median for each manufacturer and interpreted as good, ≤5%; moderate, <10%; and poor, coefficient of variation ≥10%. The coefficient of variation range described the variation in reliability and was interpreted as small, ≤5%; moderate, <10% and large, ≥10%. Inter-manufacturer agreement was represented as a Cohen d (±95% confidence interval) standardised effect size. ResultsInter-device reliability for distance, peak velocity and average acceleration was good (coefficient of variation = 0.1 to 3.9%) for both manufacturers, with small variation across sessions. For most threshold-based acceleration and deceleration counts, StatSports devices showed good to moderate reliability, with moderate variation across sessions; Catapult showed good to poor reliability, with large variation across sessions. Inter-manufacturer agreement demonstrated moderate to very large effect sizes reported for most metrics. ConclusionsReliability was suitable and consistent for measures of distance, velocity, and average acceleration. StatSports devices generally possessed suitable reliability and consistency for threshold-based accelerations and decelerations, though Catapult devices did not. Most metrics should not be compared between manufacturers.

Insufficient sampling frequencies skew heart rate variability estimates: Implications for extracting heart rate metrics from neuroimaging and physiological data

BackgroundWhile cardiac pulsations are widely present within physiological and neuroimaging data, it is unknown the extent this information can provide valid and reliable heart rate and heart rate variability (HRV) estimates. The objective of this study was to demonstrate how a slight temporal shift due to an insufficient sampling frequency can impact the validity/accuracy of deriving cardiac metrics. MethodsTwenty-two participants were instrumented with valid/reliable industry-standard or open-source electrocardiograms. Five-minute lead II recordings were collected at 1000 Hz in an upright orthostatic position. Following artifact removal, the 1000 Hz recording for each participant was downsampled to frequencies ranging 2–500 Hz. The validity of each participant’s downsampled recording was compared against their 1000 Hz recording (“reference-standard”) using Bland-Altman plots with 95 % limits of agreement (LOA), coefficient of variation (CoV), intraclass correlation coefficients, and adjusted r-squared values. ResultsDownsampled frequencies of ≥ 50 and ≥ 90 Hz produced highly robust measures with narrow log-transformed 95 % LOA (<±0.01) and low CoV values (≤3.5 %) for heart rate and HRV metrics, respectively. Below these thresholds, the log-transformed 95 % LOA became wider (LOA range: ±0.1–1.9) and more variable (CoV range: 1.5–111.6 %). ConclusionThese results provide an important consideration for obtaining cardiac information from physiological data. Compared to the “reference-standard” ECG, a seemingly negligible temporal shift of the systolic contraction (R wave) greater than 11-milliseconds (90 Hz) away from its true value, lessened the validity of the HRV. Further research is warranted to determine the minimum sampling frequency required to obtain valid heart rate/HRV metrics from pulsatile waveforms.

Discharge Characterization and Variability Determination along Shorter Sections of Soaker Hose Pipe for Soil Column Experiment

Unlike other micro-irrigation facilities like a drip, trickle, and sprinklers that emits water at regularly spaced intervals with predefined discharges, porous rubber pipes (soaker hose) has openings of variable sizes that become unevenly spaced with uneven distribution. The latter makes discharge to be variant along its lateral. Shorter sections are used under laboratory column experiments of soil wetting pattern studies and for this reason, laboratory experiments were conducted to evaluate the extent of emission rates variability on short sections of commercial Irrigation Soaker Hose, 16 mm diameter. Three sections of 10 cm length pipes were randomly selected from 15 no's cuts from different parts of the twenty meters length pipe bundle and used to investigate the extent of variability on emission rates characteristics under six different operating pressures. The result was achieved by collecting and measuring water emitted through the pipe sections at pre-determined pressures. The various discharges, coefficient of variation, and pressure-discharge curves of the section of the pipe then determined from the data. The result shows somewhat similar trends on the increase for water collected with an increase in pressures; however, when statistically compared, the discharges among the pipe sections vary. The values of Coefficient of Variation (CV) are less than 10 % as the values CV range from 0.92 % to 5.82 %, which is within a good category, according to ASAE Standard EP405.1 of 0-10%. The findings indicate that, despite variations among the investigated sections, it can use any part as a representative unit in the soil column experiments with reasonable accuracy.

Test–Retest Reliability of Functional Electromechanical Dynamometer on Five Sit-to-Stand Measures in Healthy Young Adults

Background: The purpose of this study was to determine the reliability for the strength and movement velocity of the concentric phase from the five Sit-to-Stand (5STS), using three incremental loads measured by a functional electromechanical dynamometer (FEMD) in healthy young adults. Methods: The average and peak strength and velocity values of sixteen healthy adults (mean ± standard deviation (SD): age = 22.81 ± 2.13 years) were recorded at 5, 10 and 15 kg. To evaluate the reliability of FEMD, the intraclass correlation coefficient (ICC), standard error of measurement (SEM) and coefficient of variation (CV) were obtained. Results: Reliability was high for the 10 kg (CV range: 3.70–4.18%, ICC range: 0.95–0.98) and 15 kg conditions (CV range: 1.64–3.02%, ICC: 0.99) at average and peak strength, and reliability was high for the 5 kg (CV range: 1.71–2.84%, ICC range: 0.96–0.99), 10 kg (CV range: 0.74–1.84%, ICC range: 0.99–1.00) and 15 kg conditions (CV range: 0.79–3.11%, ICC range: 0.99–1.00) at average and peak velocity. Conclusions: The findings of this study demonstrate that FEMD is a reliable instrument to measure the average and peak strength and velocity values during the five STS in healthy young adults.

Pilot Feasibility, Reliability, and Validity Assessment of Infant Skin Carotenoids Measured by Reflection Spectroscopy as a Non-invasive Biomarker of Carotenoid Intake

Abstract Objectives Skin carotenoid measurement by reflection spectroscopy offers a rapid, non-invasive assessment of carotenoid intake and status. However, feasibility, reliability, and validity have not been established in infants. We hypothesize that skin carotenoid measurement of infants’ heels and fingers will be feasible, reliable, and valid. Methods Skin carotenoid score (SCS) was measured by a modified, portable reflection spectroscopy device (Veggie Meter) at the index finger and heel of the foot in infants at 4 (n = 21), 6 (n = 12), and 8 (n = 9) months of age. Infant plasma, milk formula, and mother's breast milk carotenoid concentrations were measured by HPLC, while infant carotenoid intake was estimated from 7-day food diaries and 3 × 24-hour parent recalls. Feasibility was assessed by time to acquire triplicate reads and reliability was assessed by the coefficient of variation (CV) of triplicates and the Pearson's correlation between mean heel and finger SCS. Validity was established in 4-month-olds if skin carotenoid scores were moderately correlated (r &amp;gt; 0.5) with breast milk carotenoid concentrations during exclusive milk-feeding (n = 16) and with infant plasma carotenoid concentrations (n = 11). Results Twenty-one infants (52% female) were enrolled (47% non-Hispanic white, 29% Hispanic white, 14% African American, and 10% Asian). Triplicate heel and finger SCSs were feasibly acquired in 56 ± 11 to 87 ± 32 seconds, respectively. Heel and finger SCS reliability was similar (mean CV range 18 ± 13 to 32 ± 22%), and mean within-subject heel and finger SCSs were highly correlated (r = 0.81, P = 1 × 10−5) in 4-month-olds. Finger and heel SCSs were valid correlates of total infant plasma carotenoid concentrations (r = 0.77, P = 0.006 and r = 0.66, P = 0.026, respectively). Finger SCS significantly correlated with breast milk carotenoids (r = 0.66, P = 0.006), while heel SCS non-significantly correlated (r = 0.40, P = 0.13). Conclusions This pilot suggests that infant skin carotenoids can be feasibly measured by reflection spectroscopy and that finger SCSs are correlated with both infant plasma carotenoid concentrations and maternal breast milk carotenoid concentrations. Skin carotenoid measurement should be further investigated to facilitate the assessment of infant dietary intake and health outcomes. Funding Sources Texas Children's Hospital, USDA-ARS.

Repeatability of dynamic 3D phase-resolved functional lung (PREFUL) ventilation MR Imaging in patients with chronic obstructive pulmonary disease andhealthy volunteers.

A previous study has demonstrated the feasibility of 3D phase-resolved functional lung (PREFUL) MRI in healthy volunteers and patients with chronic pulmonary disease. Before clinical use, the repeatability of the ventilation parameters derived from 3D PREFUL MRI must be determined. To evaluate repeatability of 3D PREFUL and to compare with pulmonary functional lung testing (PFT). Prospective. Fifty-three healthy subjects and 13 patients with chronic obstructive pulmonary disease (COPD). A prototype 3D stack-of-stars spoiled-gradient-echo sequence at 1.5 T. Study participants underwent repeated MRI examination (median time interval between scans COPD/healthy subjects [interquartile range]: 7/0 days [6-8/0-0 days]) and one PFT carried out at the time of the baseline MRI. For 3D PREFUL, regional ventilation (RVent) and flow-volume loops were computed and rated by cross-correlation (CC). Also, ventilation time-to-peak (VTTP) was computed. Ventilation defect percentage (VDP) maps were obtained for RVent and CC. Repeatability of 3D PREFUL parameters was evaluated using Bland-Altman analysis, coefficient of variation (COV) and intraclass correlation coefficient (ICC). The relation between 3D PREFUL and PFT measures (forced expiratory volume in 1 second (FEV1 ) and forced vital capacity (FVC) was assessed using the Pearson correlation coefficient (r). In healthy subjects and COPD patients, no significant bias (all P range: 0.09-0.77) and a moderate to good repeatability of RVent, VTTP, and VDPRVent were found (COV range: 0.1%-18.2%, ICC range: 0.51-0.88). For CC and VDPCC moderate repeatability was found (COV range: 0.6%-43.6%, ICC: 0.38-0.60). CC, VDPRVent , and VDPCC showed a good correlation with FEV1 (all |r| > 0.58, all P < 0.05) and FEV1 /FVC ratio (all |r| > 0.62, all P < 0.05). 3D PREFUL provided a good repeatability of RVent, VTTP, and VDPRVent and moderate repeatability of CC and VDPCC in healthy volunteers and COPD patients, and correlated well with FEV1 and FEV1 /FVC. 2 TECHNICAL EFFICACY STAGE: 2.

Reliability and concurrent validity of a functional electromechanical dynamometer device for the assessment of movement velocity

The aims of the study were (i) to determine the reliability and concurrent validity of a functional electromechanical dynamometer (FEMD) to measure different isokinetic velocities, and (ii) to identify the real range of isokinetic velocity reached by FEMD for different prescribed velocities. Mean velocities were collected simultaneously with FEMD and a linear velocity transducer (LVT) in two sessions that were identical, consisting of 15 trials at five isokinetic velocities (0.40, 0.60, 0.80, 1.00, and 1.20 m·s−1) over a range of movement of 40 cm. The results obtained using each method were compared using Paired samples t-tests, Bland-Altman plots and the Pearson’s product–moment correlation coefficient, while the reliability was determined using the standard error of measurement and coefficient of variation (CV). The results indicate that the mean velocity values collected with FEMD and LVT were practically perfect correlations ( r &gt; 0.99) with low random errors (&lt;0.06 m·s−1), while mean velocity values were systematically higher for FEMD ( p &lt; 0.05). FEMD provided a high or acceptable reliability for mean velocity (CV ≤ 0.24%), time to reach the isokinetic velocity (CV range = 1.68%–9.70%) and time spent at the isokinetic velocity (CV range = 0.53%–8.94%). These results suggest that FEMD offers valid and reliable measurements of mean velocity during a fixed linear movement, as well as a consistent duration of the isokinetic phase. FEMD could be an appropriate device to evaluate movement velocity during linear movements. More studies are needed to confirm the reliability and validity of FEMD to measure different velocity metrics during more complex functional exercises.

Absolute reliability and validity of the OptoGaitTM system to measure spatiotemporal gait parameters during running

The biomechanics of walking and running, in both ground and treadmill conditions, have been extensively analysed and important differences have been reported. Despite some previous studies having examined the validity and reliability of the OptoGait™ system for measuring gait characteristics during walking, no previous works have determined the reliability and validity of this system while running on a treadmill. Therefore, this study aimed to determine the absolute reliability (within-subject variation) and evaluate the concurrent validity of the OptoGait™ system for measuring spatiotemporal variables while running at a comfortable speed by comparing data with a highly accurate system of measuring those parameters (i.e. video analysis at 1000 Hz). Forty-nine endurance runners performed a running protocol on a treadmill at a comfortable speed. Two systems were used to collect data: OptoGait™ system and high-speed video analysis at 1000 Hz. The coefficient of variation (CV) was calculated as a measure of absolute reliability. The OptoGait™ system reported a CV range between 2.2% and 11.4% for spatiotemporal parameters, while the video analysis showed a CV range between 0.02% and 9.9%. To determine concurrent validity, intra class correlation coefficients (ICC) and pairwise comparisons of means (t-test) were calculated between data from both systems. Although the paired t-test demonstrated significant differences between systems, a high level of agreement (ICC &gt; 0.89) was obtained in spatiotemporal parameters between systems. When compared to a high-speed video analysis at 1000 Hz, the results indicate that the OptoGait™ system is a reliable and valid tool to measure spatiotemporal gait characteristics while running on a treadmill at a comfortable speed.

Impaired insulin secretion predicting unstable glycemic variability and time below range in type2 diabetes patients regardless of glycated hemoglobin or diabetes treatment.

Aims/IntroductionTo identify the coefficient of variation (CV) threshold for unstable glucose variability (GV) and hypoglycemia, and to characterize a patient population with unstable GV and hypoglycemia.Materials and MethodsThis was an observational study that enrolled 284 Japanese outpatients with type 2 diabetes who underwent continuous glucose monitoring. The C‐peptide index (CPI = [(fasting serum C‐peptide) / (plasma glucose)] × 100) was used as a marker of endogenous insulin secretion. The CV threshold between stable and unstable GV was defined as the upper limit of the CV distribution in the subgroup of patients who did not receive insulin nor insulin secretagogues (relatively stable GV subgroup, n = 104). The optimal CV range corresponding to time below target range ≥4% was determined for all patients using receiver operating characteristic curve analysis. Various characteristics of patients with unstable GV and hypoglycemia were extracted using multivariate logistic regression analysis.ResultsThe upper limit of the CV in the relatively stable GV subgroup was 40. The optimal CV range corresponding to time below target range ≥4% was also defined as CV ≥40 (area under the curve 0.85) for all patients. The CPI was an independent risk for CV ≥40 (odds ratio 0.17, 95% confidence interval 0.04–0.50, P < 0.01). The optimal cut‐off point for CPI to predict a CV cut‐off value of 40 was equivalent to 0.81 (area under the curve 0.80).ConclusionsA CV of 40 discriminates unstable GV and hypoglycemia from stable GV in Japanese outpatients with type 2 diabetes. Impaired insulin secretion might affect the stability of GV.

Evaluation of serological assays to monitor antibody responses to single-dose HPV vaccines

IntroductionWhether existing serological assays are sufficiently robust to measure the lower antibody levels expected following single-dose HPV vaccination is unknown. MethodsWe evaluated seven assays measuring HPV-16/18 immunological responses overall and by number of doses in 530 serum samples from participants receiving varying doses of Cervarix or Gardasil up to 36-months post-vaccination. Serum was evaluated by simplex (HPV-16 ELISA, HPV-18 ELISA), multiplex (LIA-4, VLP-MIA, M9ELISA, GST-L1), and high-throughput pseudovirion-based neutralization assays (HT-PBNA), and results were compared to the gold standard HPV-16/18 secreted alkaline phosphatase neutralization assay (SEAP-NA). Reproducibility was assessed by the coefficient of variation (CV) and intraclass correlation coefficient (ICC). Percent agreement, Pearson correlation, and weighted-kappa were used to assess validity. Determinants of seronegativity were evaluated by chi-squared test. ResultsHPV-16: Seropositivity range was 97.1–99.5% for single dose and 98.8–99.8% overall. CV range was 4.0–18.0% for single dose and 2.9–19.5% overall. ICC range was 0.77–0.99 for single dose and 0.74–0.99 overall. Correlation with SEAP-NA range was 0.43–0.85 for single dose and 0.51–0.90 overall. Weighted-kappa range was 0.34–0.82 for single dose and 0.45–0.84 overall. HPV-18: Seropositivity range was 63.9–94.7% for single dose and 86.2–97.9% overall. CV range was 8.1–18.2% for single dose and 4.6–18.6% overall. ICC range was 0.75–0.99 for single dose and 0.83–0.99 overall. Correlation with SEAP-NA range was 0.31–0.99 for single dose and 0.27–0.96 overall. Weighted-kappa range was 0.35–0.83 for single dose and 0.45–0.84 overall. HPV-16 seronegativity was <5% for all assays. HPV-18 seronegativity range was 5.5–17.3%. For LIA-4 and GST-L1 where the proportion of seronegativity was >10%, the strongest correlates of seronegativity were receiving a single vaccine dose and receiving Gardasil. Conclusions:These results support the utility of existing serological assays to monitor antibody responses following single-dose HPV vaccination.

Session-To-Session Variations of External Load Measures of Youth Soccer Players in Medium-Sided Games.

The aim of this study was to analyze the variability of time-motion variables during five vs. five games when completed within the same session as, and between, two different sessions. Ten under-19 male soccer players (18.27 ± 0.47 years old) participated in this study. The five vs. five matches (3 × 5 min) were played twice with a 3-day interval of rest in the same week. Moderate between-session variations were observed for TD (total distance) (range coefficient of variation (CV), 6.9; 8.3%, confidence interval (CI), (5.0; 14.0), standardized typical error (STE), 0.68; 1.06, (0.64; 1.75)) and RD (running distance) (range CV, 53.3; 145.7%, (36.6; 338.9), STE, 0.83; 1.09, (0.60; 1.76)). PL (player load) showed small variations (range CV, 4.9; 6.0%, [3.6; 10.1], STE, 0.37; 0.43, (0.27; 0.71)). In within-session analyses for examining the differences between sets, a small decrease was observed in RD in set 3 versus set 2 (−14.8%, 90% CI (−32.1; 6.9%); standardized difference (ES): −0.39 (0.95; 0.16)). TD decreased with moderate (−3.5%, (−6.8; −0.1%); ES: −0.65(−1.30; −0.01)) and large (−8.2%, (−11.4; −4.9%); ES: −1.58(−2.24; −0.92)) effects in sets 2 and 3, respectively, versus set 1. Our results suggest that PL is the most stable performance variable. It was also verified that measures had a progressive decreasing tendency within a session.

The addition of very light loads into the routine testing of the bench press increases the reliability of the force-velocity relationship.

BackgroundThe aim of this study was to examine whether the addition of very light loads for modeling the force–velocity (F–V) relationship during the bench press (BP) exercise can confirm its experimental linearity as well as to increase the reliability and concurrent validity of the F–V relationship parameters (maximum force (F0), maximum velocity (V0), F–V slope, and maximum power (Pmax)).MethodThe F–V relationship of 19 healthy men were determined using three different methods: (I) 6-loads free method: six loads performed during the traditional free-weight BP exercise (≈ 1–8–29–39–49–59 kg), (II) 4-loads free method: four loads performed during the traditional free-weight BP exercise (≈ 29–39–49–59 kg), and (III) 4-loads Smith method: four loads performed during the ballistic bench press throw exercise in a Smith machine (≈ 29–39–49–59 kg).ResultsThe linearity of the F–V relationship was very high and comparable for the three F–V methods (p = 0.204; median Pearson’s correlation coefficient (r) = 0.99). The three methods were ranked from the most to the least reliable as follows: 6-loads free (coefficient of variation (CV) range = 3.6–6.7%) > 4-loads Smith (CV range = 4.6–12.4%) > 4-loads free (CV range = 3.8–14.5%). The higher reliability of the 6-loads free method was especially pronounced for F–V slope (CVratio ≥ 1.85) and V0 (CVratio ≥ 1.49) parameters, while the lowest difference in reliability was observed for F0 (CVratio ≤ 1.27). The 6-loads free and 4-loads free methods showed a very high concurrent validity respect to the 4-loads Smith method for F0 and Pmax (r ≥ 0.89), a moderate validity for the F–V slope (r = 0.66–0.82), and a low validity for V0 (r ≤ 0.37).DiscussionThe routine testing of the F–V relationship of upper-body muscles through the BP exercise should include trials with very light loading conditions to enhance the reliability of the F–V relationship.

Intra-session and Inter-day Reliability of the Myon 320 Electromyography System During Sub-maximal Contractions.

Electromyography systems are widely used within the field of scientific and clinical practices. The reliability of these systems are paramount when conducting research. The reliability of Myon 320 Surface Electromyography System is yet to be determined. This study aims to determine the intra-session and inter-day reliability of the Myon 320 Surface Electromyography System. Muscle activity from fifteen participants was measured at the anterior deltoid muscle during a bilateral front raise exercise, the vastus lateralis muscle during a squat exercise and the extensor carpi radialis brevis (ECRB) muscle during an isometric handgrip task. Intra-session and inter-day reliability was calculated by intraclass correlation coefficient, standard error of measurement and coefficient of variation (CV). The normalized root mean squared (RMS) surface electromyographic signals produced good intra-session and inter-day testing intraclass correlation coefficient values (range: 0.63–0.97) together with low standard error of measurement (range: 1.49–2.32) and CV (range: 95% Confidence Interval = 0.36–12.71) measures for the dynamic-and-isometric contractions. The findings indicate that the Myon 320 Surface Electromyography System produces good to fair reliability when examining intra-session and inter-day reliability. Findings of the study provide evidence of the reliability of electromyography between trials which is essential during clinical testing.

Anterior segment ultrasound biomicroscopy image analysis using ImageJ software: Intra-observer repeatability and inter-observer agreement.

In this novel study, we demonstrate a standardized imaging and measurement protocol of anterior segment (AS) structures with reliability analysis using ultrasound biomicroscopy (UBM) and ImageJ software. Ten pediatric and young adult patients undergoing examination under anesthesia for AS pathology were imaged using UBM. Four trained observers analyzed 20 images using ImageJ. Forty-five structural parameters were measured. Those that relied on the trabecular-iris angle (TIA) as a reference landmark were labeled TIA-dependent (TD) and all others were labeled non-TIA dependent (NTD). Intra-observer repeatability (IOR) and inter-observer agreement (IOA) of measurements were determined using coefficient of variation (CV) and intra-class correlation (ICC) followed by assessment of Bland-Altman plots (BAP) for each pair of observers, respectively. For NTD parameters, non-ciliary body (CB) related measurements showed CV range 0.60-16.22% and ICC range 0.84-0.89, whereas CB-related parameters showed CV range 2.86-23.40% and ICC range 0.29-0.92. For TD parameters, parameters < 2 degrees removed from reference showed CV range 0.02-5.40% and ICC range 0.89-1.00, whereas parameters > 1 degree removed showed CV range 0.63-27.44% and ICC range 0.22-1.00. No systematic proportional bias was detected by BAPs. Preplaced landmarks yielded good IOR and IOA in quantitative assessment of AS structures that were NTD and non-CB-related or less removed from the reference. CB-related NTD measurements varied greatly in IOR and IOA, indicating protocol modifications or CB qualitative assessments needed to improve accuracy. Variability in TD measurements increased the further removed from the reference, which supports implementation of a reliable reference landmark to minimize variation.