Cod Liver Research Articles

Extraction and encapsulation of squalene-rich cod liver oil using supercritical CO2 process for enhanced oxidative stability

We produced squalene-rich cod liver oil (CLO) and solid-lipid particles (SLP) were produced using supercritical carbon dioxide (SC-CO2) extraction and gas saturated solutions (PGSS) processes respectively. Polyethylene glycol (PEG) was used for encapsulation. The encapsulation yield and efficiency (EE) of the SLPs obtained from the oil under 25 MPa, 4:20 (SCO:PEG, w/w) condition, were 71.13 % ± 0.44 % and, EE 35.52 % ± 0.22 %, respectively. The optimum conditions included 25 MPa, 4:20 (SCO:PEG, w/w), the bulk density and tapped density of the SLPs were 0.30 ± 0.02 and, 0.33 ± 0.02 g/cm3, respectively. The corresponding Carr’s index (CI) value was the lowest at 9.09 ± 0.97 and the smallest particle size was 315.83 µm with 9-PEG, making it suitable for commercial applications. Microencapsulated CLO was characterized using scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimeter (DSC). Upon evaluation, the antioxidant activity of squalene was enhanced with 9-PEG at ABTS+ 142.60 ± 5.71 mgTE/g and, DPPH 30.38 ± 1.44 mgTE/g. Encapsulated CLO (22.50 ± 0.30 meq/kg) showed improved oxidative stability than bare CLO. Therefore, encapsulation of functional oils obtained via SC-CO2 extraction using PGSS process inhibits oxidation and facilitates its commercial applications.

Consumption of Cod Liver Oil-enriched Vernonia amygdalina Leaf-based Diet Promoted Wound Healing in Wound-inflicted Type 2 Diabetic Wistar Rats

Consumption of Cod Liver Oil-enriched Vernonia amygdalina Leaf-based Diet Promoted Wound Healing in Wound-inflicted Type 2 Diabetic Wistar Rats

Consumption of Cod Liver Oil-enriched Vernonia amygdalina Leaf-based Diet Promoted Wound Healing in Wound-inflicted Type 2 Diabetic Wistar Rats

Consumption of Cod Liver Oil-enriched Vernonia amygdalina Leaf-based Diet Promoted Wound Healing in Wound-inflicted Type 2 Diabetic Wistar Rats

ANTIOXIDANT PROPERTIES OF TRASH FISH Odonus niger LIVER OIL IN COMPARISON WITH COD LIVER OIL

The present study was undertaken to compare the in vitro antioxidant properties of marine trash fish Odonus niger liver oil and commercially available cod liver oil. For this O. niger fishes were collected and the oil was extracted from the liver mass by following Bligh and Dyer method and it was purified. Further the antioxidant potential of various concentrations (12.5 to 200µg/ml) of the extracted oil was performed through different standard antioxidant assays, simultaneously the same concentrations of commercial cod liver oil were also performed. The antioxidant potential of both oils was compared with appropriate standards like ascorbic acid, gallic acid and quercetin in the respective assays. The different assays performed were hydroxyl radical scavenging activity (23.24%), total antioxidant activity (43.06%), DPPH radical scavenging activity (60.06%), reducing power effect (68.22%), lipid peroxidation inhibitory activity (53.10%) and ferrous ions chelating activity (75%) respectively for O. niger at the highest concentration of 200μg/ml. All the above activities showed that O. niger liver oil possessed better antioxidant potential, especially at higher concentration (200 µg/ml) than the tested commercial cod liver oil.

Correlation between intake of fish or supplements containing omega-3 fatty acids and early pregnancy plasma concentrations.

Long-chain polyunsaturated omega-3 fatty acids are considered important for fetal development, but previous studies suggest suboptimal intake in part of pregnant women in Iceland. The study aim was to evaluate intake of food and supplements containing omega-3 fatty acids, among pregnant women in Iceland and correlations to fatty acid composition in plasma. Subjects were 853 pregnant women attending their 11-14 weeks ultrasound appointment. Information on intake of food and supplements containing long-chain omega-3 fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) as well as background was obtained by a questionnaire. Blood samples were collected for analysis of plasma fatty acids. Correlation was evaluated using the Spearman correlation. Median intake of lean fish was 1.3 times per week, while fatty fish was consumed once monthly. About 50% of the women took omega-3 containing supplements daily. Higher intake of both fish and omega-3 containing supplements was reflected in higher omega-3 plasma levels (r=0.37 p<0.001). A positive correlation was seen between intake of cod liver oil/capsules (r=0.23, p=0.001), omega-3 oil/capsules (r=0.20, p=0.001) and plasma concentration of omega-3. However, no correlation was seen between intake of a maternal multivitamin containing omega-3 and corresponding plasma concentration (r=0.03, p=0.98). Intake of food and supplements containing omega-3 fatty acids was reflected in plasma concentration, except for an Icelandic maternal multivitamin. One third of the women followed the recommendation of eating fish at least twice weekly. About 50% had a daily intake of supplements containing omega-3 fatty acids.

Cod Liver Oil's Encapsulation into Sodium Alginate/Lupin Protein Beads and Its Application in Functional Meatballs' Preparation.

Cod liver oil (CLO) is an essential source of healthy ω-3 fatty acids to be employed in functional meals. However, its autoxidation sensitivity, solubility, and odour present it as challenging to handle. Its encapsulation might mitigate these problems. This research studied using alginate/lupine protein as a wall material for CLO encapsulation as well as to characterise CLO microcapsules for their size, sphericity factor, encapsulation efficiency, morphology (scanning electron microscopy), in vitro release, and thermal stability. In this study, the oxidative stability, quality parameters, and sensory attributes of meatballs enriched with free CLOs and encapsulated CLOs throughout storage at 4 ± 1 °C for 16 days were assessed. The CLO microspheres had a homogeneous round shape, a diameter of 0.82 ± 0.06 mm, a sphericity factor of 0.092 ± 0.01, an encapsulation efficiency of 95.62% ± 1.13%, and an accumulative release rate of 87.10% after 270 min in the stimulated gastrointestinal conditions. Additionally, it was discovered that encapsulated oil was more stable than free CLOs to heat treatments (70–100 °C, 24 h). pH, thiobarbituric acid-reactive substances, peroxide value, conjugated dienes value, and carbonyl content of meatballs enriched with microencapsulated CLOs were significantly lower when compared to free CLOs and/or control samples. CLO microcapsules improved the sensory characteristics of meatballs throughout storage; however, meatballs directly containing CLOs were rejected. Thus, the viability of alginate/LPI complex microcapsules containing CLOs to enrich meat products subjected to storage with refrigeration could be indicated without any change in the characteristics.

Fish Oil Replacement by a Blend of Vegetable Oils in Diets for Juvenile Tench (Tinca tinca Linnaeus, 1758): Effects on Growth Performance and Whole-Body Composition.

Simple SummaryFish oil (FO) can be totally replaced by a blend of vegetable oils (30% linseed oil, 20% corn oil, and 50% olive oil) in diets for juvenile tench (Tinca tinca Linnaeus, 1758) without negative effects on survival rate and growth performance. Diets affected the composition of juveniles so that lipid content was significantly lower in animals fed diets without FO. Moreover, the content of linolenic acid (18:3n-3) increased as FO substitution did, being significantly higher with respect to control diet (without vegetable oils) from 40% or higher FO replacement diets. No differences in saturated, monounsaturated, and polyunsaturated fatty acids were found in the fish whole-body. Nutritional indices can be considered within optimal values for healthy foods.Among freshwater species, tench (Tinca tinca Linnaeus, 1758) is considered as a promising species for the diversification of aquaculture, but the intensification of techniques is necessary to promote and consolidate its culture. Adequate feeding in early growth phases is essential to face further grow-out. Fish oil (FO) is the main source of lipids in fish diets, but its production is unsustainable, and thus, alternative oils should be considered. A 90-day experiment was performed to evaluate the effects of partial and total replacement of cod liver oil (FO) by a blend of vegetable oils (VO) in juvenile tench. Six isonitrogenous and isolipidic diets with different levels of a VO blend containing 30% linseed oil, 20% corn oil, and 50% olive oil were tested: 0% (control), 20%VO, 40%VO, 60%VO, 80%VO, and 100%VO. With all diets, survival was 100%, and there were not differences in growth performance (total length (TL); weight (W); specific growth rate (SGR); feed conversion ratio (FCR); and biomass gain (BG)). Compared to the control group, whole-body lipid content decreased significantly in the 100%VO group. No differences in total saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids were found in the whole-body. The content of linolenic acid (ALA) in the whole-body increased as FO substitution did, being significantly higher with respect to control diet from 40% FO replacement diets. Nutritional indices, such as ΣPUFA/ΣSFA and Σn-6/Σn-3 ratios, tended to increase with increasing VO content, whereas the EPA + DHA showed an opposite trend. A total replacement of FO by the blend of VO did not affect the growth performance and fatty acid profile of juvenile tench. Further research on the effects of VO diet on nutritional quality in tench reared to commercial size should be performed.

Experimental study on the relationship between foam pressure difference and foam stability

Foam stability affects the efficacy and incidence of side effects of foam sclerotherapy. Exploring the relationship between foam pressure difference and foam stability can provide ideas and basis for obtaining more stable foam. In the experiment, sodium cod liver oleate foam was selected, and poloxamer 188 (concentration of 0%, 4%, 8%, 12%) was added to realize the change of foam pressure. By using the self-written program to process the foam pictures, the foam pressure difference and the relationship between the foam stability indicators (water separation rate curve, half-life) and the foam pressure difference were obtained. The results showed that at first the foam pressure increased with the increase of the concentration, and then it decreased with the increase of the concentration and reached a peak at the concentration of 4%. The foam pressure difference decreases continuously with the increase of decay time. When the additive concentration is low, the foam average pressure difference increases. And if the additive concentration is too high, the foam average pressure difference decreases. The smaller the foam pressure difference is, the better the foam stability is. This paper lays a foundation for the research on the stability of foam hardener.

Pharmacological evaluation of the effects of enzymatically liberated fish oil on eosinophilic inflammation in animal models.

The inappropriate activation of eosinophils is a well-recognized driver of various human inflammatory diseases including asthma, chronic rhinitis, and various gastrointestinal diseases, including eosinophilic esophagitis. Steroids, both topical and systemic, remain a cornerstone of treatment and can be highly effective. However, some individuals suffer side effects, unresolved symptoms, or both. OmeGo, an enzymatically liberated fish oil, has demonstrated anti-inflammatory and antioxidant properties as well the reduction of the activation, migration, and survival of eosinophils. Two animal models of eosinophilic inflammation were used to further assess OmeGo's profile. A house dust mite model of induced asthma showed a significant reduction in eosinophilic lung inflammation compared to the negative control, linoleic acid. The CRTH2 antagonist fevipiprant showed a similar eosinophilic inhibitory profile to OmeGo. In contrast, cod liver oil had no impact on any measure of inflammation. A guinea pig model of mild intraperitoneal eosinophilia showed a significant reduction in eosinophil activity by OmeGo, assessed by chemotaxis and chemokinesis. Apolipoprotein A-IV, an endogenous human protein with anti-inflammatory actions, showed a similar but numerically lower effect. OmeGo therefore combines a consistent antieosinophilic action with the known anti-inflammatory effects of polyunsaturated fatty acids. Proof-of-concept studies in asthma are warranted.

Docosahexaenoic Acid-Enhanced Autophagic Flux Improves Cardiac Dysfunction after Myocardial Infarction by Targeting the AMPK/mTOR Signaling Pathway.

Background and Purpose. Docosahexaenoic acid (DHA) is a type of polyunsaturated fatty acid enriched in cod liver oil and seaweed. It is necessary for the human body and has important functions, such as antioxidation and antiatherosclerosis activities. Long-term oral administration of DHA or the use of DHA at the initial stage of ischemia can increase the level of autophagy and exert a protective effect on neurological functions related to cerebral infarction. However, the effect of DHA on myocardial injury and cardiac insufficiency after myocardial infarction (MI) is unknown. This study was aimed at exploring whether DHA plays a protective role in AMI and its specific molecular mechanism. Experimental Method. In vitro cardiomyocyte hypoxia and in vivo MI injury models were used to determine the role of DHA in MI. Hypoxic injury induced damage in cultured neonatal mouse cardiomyocytes (NMCs). The C57BL/6J mouse MI model was established by permanent ligation of the left anterior descending branch. Main Results. DHA improved the cardiomyocyte viability of NMCs induced by hypoxia injury and reduced cell necrosis. DHA reduced infarct size, improved heart function, and reduced the degree of myocardial fibrosis in mice after MI. In addition, DHA enhanced autophagy flux and reduced apoptosis in vitro and in vivo. In addition, we found that chloroquine, an autophagy inhibitor, blocked the protective effect of DHA on cardiomyocyte apoptosis and cardiac dysfunction, indicating that DHA exerts cardioprotective effects in part by promoting autophagy flux. We also observed that DHA enhanced autophagy flux by activating the AMPK/mTOR signaling pathway. Conclusions and Significance. In conclusion, our findings indicate for the first time that DHA improves MI-induced cardiac dysfunction by promoting AMPK/mTOR-mediated autophagic flux.

Risk factors for vitamin D deficiency in Abu Dhabi Emirati population.

There have been concerns regarding the high prevalence of vitamin D deficiency in the United Arab Emirates and the association between this prevalence and cardiovascular risk. Vitamin D deficiency is a global public health problem, with a high prevalence in the Abu Dhabi national population. This cross-sectional retrospective observational study aimed to elucidate vitamin D deficiency symptoms and contributing factors in this context. Among 12346 participants presenting for their Weqaya population comprehensive cardiovascular risk factor screening, 700 were randomly selected and telephoned, among whom 400 completed a questionnaire that elicited information on dietary intake, sun exposure, clothing, physical activity, and symptoms related to vitamin D deficiency. Higher vitamin D levels were noted in participants who consumed extra servings of cod liver oil, tuna, salmon, and eggs; who wore clothes with their arms and legs exposed; and who performed daily physical activity. Symptoms of hypovitaminosis D (bone pain) were more frequently found in females. Fatigue is significantly associated with low vitamin D and younger participants. Strategies focusing on health promotion and lifestyle interventions should be implemented to address vitamin D deficiency.

Concentration of Bioactive and Mineral Compounds in Enteral Tube Feed Products Made of Plant-Based Ingredients

Abstract Nowadays available products for enteral nutrition are supplemented with synthetic ingredients. The effectiveness of these products is undeniable in the medical industry, but there is concern about bioavailability of synthetic vitamin and mineral compounds in comparison to naturally occurring ones. The aim of this study was to create plant-based formulations for enteral tube feed and to determine their bioactive compound and mineral compound concentration in comparison with the values recommended by the European regulations for special medical purpose food that is not meant to satisfy the nutritional requirements of infants. For this study, five enteral tube feed formulas were made from fruit, berry, and vegetable semi-finished products with added whey protein isolate, canola oil, cod liver oil, iodised salt, and ascorbic acid. The samples were thermally processed and concentration of vitamin C, total carotenes, total phenols and mineral compounds: P, K, Na, Ca, Mg, Fe, Mn, Zn, Cu and Se was determined. All samples showed &lt; 20 mg·kg−1 per sample of Se, and also concentration of Zn was under the (EU) 2016/128 requirements. However, concentration of Mg (345.2 to 420 mg·100 kcal−1 of product) and K (29.2 to 39.2 mg·100 kcal−1 of product) exceeded the maximum levels. Also, the concentration of vitamin C exceeded the maximum, by average two times per sample, as L-ascorbic acid was added in response to previous experiments showing unsatisfying levels.

Medication Adherence, Complementary Medicine Usage and Progression of Diabetic Chronic Kidney Disease in Thais.

PurposeNon-adherence to medication is receiving more attention as a significant problem common to management of chronic diseases including diabetes and chronic kidney disease (CKD). This study was designed to assess the medication adherence and self-medication in a cohort of Thai patients with diabetic kidney disease, and its association with clinical outcomes.Patients and MethodsNon-dialysis patients with diabetic CKD visiting outpatient’s clinics of Siriraj Hospital, the largest tertiary care in Thailand, were asked for participation. Self-administered questionnaire was given to assess medication adherence (the 6-item-medication-taking-behavior measure in Thai), complementary medicine usage, and personal information. Clinical, pharmaceutical, and relevant laboratory data (at current and the last visit of around 12 months) were abstracted from the medical records.ResultsOf the 220 participants eligible (54.1% male, mean age 71.3), 50.9%, 24.1%, and 25% were classified as high-, medium-, and low-medication adherence, respectively. Overall, 24.1% reported self-usage of at least one type of herbal or complementary medicines. The most commonly identified items were cordyceps, cod liver oil, Nan Fui Chao, and turmeric (6 each), with unidentified Thai herbal mixture in 11. On multivariate analysis, late-stage CKD (stage IV–V) was the only independent predictor for low adherence (odds ratio (OR), 5.54; 95% confidence interval (CI), 2.82–10.88). Low adherence was associated with higher blood pressure, lower estimated glomerular filtrate rate (eGFR), and more eGFR decline with greater risk of being rapid CKD progressor (annual eGFR drop >5 mL/min/1.73 m2) [OR, 1.15; 95% CI, 1.06–1.25].ConclusionMedication taking behavior was a frequently encountered problem in Thai diabetic CKD patients. Increased medication non-adherence was independently predicted by stages of increasing CKD severity, and it was associated with poorer hypertensive control and kidney outcome. Targeting interventions to improve medication adherence should be an important strategy to slow CKD progression among patients with diabetic CKD.

Cod Liver Oil, but Not Retinoic Acid, Treatment Restores Bone Thickness in a Vitamin A-Deficient Rat.

Vitamin A plays a prominent role for maintaining optimal bone status, but its impact upon the bone in response to vitamin A deficiency is not well defined. The purpose of this study was to evaluate how replenishing vitamin A by either whole food cod liver oil (COD) or the active metabolite of vitamin A, retinoic acid (RA), altered bone thickness of vitamin A-deficient (VAD) rats. Weanling rats were administered a control diet (CTRL) or VAD diet for 9 weeks. This was followed by four weeks of treatment in which the VAD group was divided into the following 4 subgroups: (1) VAD (9 weeks)-VAD (4 weeks); (2) VAD-CTRL; (3) VAD-COD; and (4) VAD-RA. Compared to controls, VAD rats had thicker bones which showed marked dysplasia. VAD-rats treated with COD produced a thinner bone that was not significantly different from that of untreated rats. In contrast, RA did not significantly change the thicker bone, and also had significantly greater periosteal and endosteal osteoblast numbers compared to VAD-COD. Active osteoclasts were not detected in VAD rats, nor during the treatment period. These findings suggest that the abnormal bone thickness in VAD rats appears to be more effectively restored to bone thickness of untreated control rats when treated with COD.

Immune responses and acute inflammation in common carp Cyprinus carpio injected by E.coli lipopolysaccharide (LPS) as affected by dietary oils

Eicosanoids, resolvins, and lipoxins formed from long-chain polyunsaturated fatty acids (LC-PUFAs) are the main lipid mediators in the inflammatory processes explaining the influence of dietary lipid sources on the immune system. The current study aimed to determine the effects of dietary plant oils instead of fish oil or LC-PUFA supplementation in these oils on fish immune and inflammatory responses under normal and LPS-stimulated conditions. Six iso-nitrogenous (ranging from 30.4 to 31.1%) and iso-lipidic (from 11.2 to 11.6%) diets were formulated using three oil sources: cod liver oil (CLO, as fish oil control); linseed oil (LO, rich in α-linolenic acid, ALA); sesame oil (SO, rich in linoleic acid, LA); a blend of LO and SO (SLO, v:v 1:1); and two pure plant oil diets supplemented with docosahexaenoic acid, DHA (SO + DHA, SOD) or arachidonic acid, ARA (LO + ARA, LOA). Fish were fed the experimental diets to satiation for 42 days. On day 43, they were injected with E.coli lipopolysaccharide (LPS) at 100 μg/fish. Fish plasma and tissues such as head kidney and liver were collected on day 42 and one day after LPS injection (day 44) for humoral immune variables and gene expression analyses, respectively. After 42 days of feeding, no influences of dietary oils were found on fish survival, growth, feed utilization, and humoral immune responses. On the other hand, LPS injection significantly stimulated immune responses and induced an acute inflammation in common carp through an increase of the complement activity and the up-regulation of genes involved in the innate immune system (c2), pro-inflammatory response (tlr-4, tnf-α, il-1, il-6, il-8, and cxc), eicosanoid metabolism (pla2, cox-1, 5-lox, and pge2), and anti-inflammatory response (tgf-β1 and nf-fki). Further, the expression of hsp70 was stimulated by LPS injection. The effects of dietary oil sources were observed after LPS injection, with a significant modification in the expression of almost all candidate genes. The highest pro-inflammatory responses induced by LPS were observed in CLO-fed fish while the mixture of plant oils (SLO) and LC-PUFA-supplemented diets induced significantly higher modulations in anti-inflammatory responses (il-10 and nf-kbi), general stress status (hsp70), and cytoprotection (gpx-1) compared to fish oil control and other pure plant oil groups. In conclusion, the immune response of common carp has been modified by the dietary fat sources. The fish oil-based diet supported an increase of the pro-inflammatory responses while the mixture of plant oil or LC-PUFA supplemented diets improved the anti-inflammatory responses and cytoprotection.

Oxidative damage in Nile tilapia, Oreochromis niloticus, is mainly induced by water temperature variation rather than Aurantiochytrium sp. meal dietary supplementation.

We investigated whether dietary supplementation with Aurantiochytrium sp. meal, a DHA-rich source (docosahexaenoic acid, 22: 6 n-3), fed during long-term exposure to cold-suboptimal temperature (22°C, P1), followed by short-term exposure to higher temperatures (28°C, P2, and 33°C, P3), would promote oxidative damage in Nile tilapia (Oreochromis niloticus). Two supplementation levels were tested: 1.0g 100g-1 (D1) and 4.0g 100g-1 (D4). A control diet, without the additive (D0, 0g 100g-1), and a positive control diet supplemented with cod liver oil (CLO) were also tested. The concentrations of DHA and total n-3 PUFAs in the CLO diet were similar to those found in diets D1 and D4, respectively. The parameters analyzed included hemoglobin (Hb), the antioxidant enzymes catalase, glutathione peroxidase, total glutathione, non-protein thiols, and the oxidative markers protein carbonyl and erythrocyte DNA damage. Nile tilapia did not present differences in Hb content, regardless of diet composition, but the temperature increase (P1 to P2) led to a higher Hb content. Likewise, the temperature increases promoted alterations in all antioxidant enzymes. The dietary supplementation with 1.0g 100g-1 Aurantiochytrium sp. meal after P1 caused minor DNA damage in Nile tilapia, demonstrating that the additive can safely be included in winter diets, despite its high DHA concentration.

Technological effect of dietary oat fiber on the quality of minced sausages prepared from Indian major carp (Labeo rohita)

The development of functional meat sausages with new sources of fiber ingredients is progressing day by day. In this study, functional sausages developed with Indian major carp, Rohu fish mince that could increase dietary fiber intake through consumption. Different levels of oat fiber (2.5%, 5%, 7.5% and 10% w/w) was incorporated in fish mince emulsified with 8% (w/w) cod liver oil and 8% (w/w) starch. Adding oat fiber resulted in reduced moisture content in all samples regardless of fiber content, and a significant (p < 0.05) influence was noticed on the gel strength, WHC, and folding score of sausages. The electrophoretic pattern of total proteins showed polymerization in the presence of oat fiber, but this did not affect the protein patterns of minced sausages. The scanning electron microscopy revealed a good compact gel network in the presence of oat fiber. The sausages retained good textural quality except for variation in hardness. The whiteness of the sausages was reduced, and yellowness increased due to the slight yellow colour of the oat fiber. A higher level of oat fiber inclusion resulted in slight oats odour development. Overall, fortification of minced sausages with oat fiber at the level of 2.5% (w/w) is recommended. The fortification of minced sausages with fiber containing starch and cod liver oil resulted in a good quality product that could be useful in developing mince-based fish products with nutritional benefits.

Profiling of Unsaturated Lipids by Raman Spectroscopy Directly on Solid-Phase Microextraction Probes.

Lipids play a critical role in cellular signaling, energy storage, and the construction of cellular membranes. In this paper, we propose a novel on-site approach for detecting and differentiating enriched unsaturated lipids based on the direct coupling of SPME probes with Raman spectroscopy. To this end, different SPME particles, namely, hydrophilic-lipophilic balanced (HLB), mixed-mode (C8-SCX), and C18, were embedded in polyacrylonitrile (PAN) and tested for their efficacy as biocompatible coatings. The C18/PAN coating showed less background interference compared to the other sorbent materials during the analysis of unsaturated lipids. In addition, different SPME parameters that influence extraction efficiency, such as extraction temperature, extraction time, and washing solvent, were also investigated. Our results indicate a clear dependence between the Raman band intensity related to the number of double bonds in fatty acids mixture and the number of double bonds in a fatty acid. Our findings further show that Raman spectroscopy is especially useful for the analysis of lipid unsaturation, which is calculated as the ratio of n(C═C)/n(CH2) using the intensities of the Raman bands at 1655/1445 cm-1. Furthermore, the developed protocol reveals great SPME activity and high detection ability for several unsaturated lipids in different complex matrixes, such as cod liver oil. Finally, the applicability of this technology was demonstrated via the characterization of cod liver oil and other vegetable oils. Thus, the proposed SPME-Raman spectroscopy approach has a great future potential in food, environmental, clinical, and biological applications.

Pain Management by Ayurveda in Lumbar Spondylosis– A Case Study and Selective Review of Literature

Introduction: In Ayurveda, Snehana is an important method of treatment for diseases of the spine. As per Ayurveda, fatty substances are having properties opposite to that of Vata Dosha which is the root of many diseases. Therefore, Sneha (fatty substances) have an important position in Ayurvedic treatment. Fatty substances are of 4 types of Ghrita (Ghee), Taila (oil), Vasa (muscle fat), and Majja (bone marrow fat).&#x0D; Among these types, the literature is available about Ghrita (ghee), Taila (oil), and Majja (bone marrow fat) only, but Vasa (muscle fat) is not currently practiced. We got a case study, in which cod liver oil capsules were used as a representative of Vasa Sneha.&#x0D; A male of 35 suffering from lumbar spondylosis approached for moderate to severe low back pain. We successfully used cod liver oil capsules as representative of Vasa Sneha in this case.&#x0D; Study Design: A single case study and selective review of the literature.&#x0D; Place and Duration of Study: Panchakarma OPD, Yashwant Ayurvedic medical college, PGTRC, Kodoli, 416114, Dist. – Kolhapur, Maharashtra, India.&#x0D; Presentation of Case: This is a single case study in which a male of 35 years was suffering from chronic low back pain (CLBP) due to lumbar spondylosis The patient had chronic low back pain which was aggravated for 3 months.&#x0D; We prescribed him Ayurvedic medicines and cod liver oil capsules orally. The patient was followed for 16 months.&#x0D; Results: The outcome measure was assessed by the Oswestry low back pain disability questionnaire. The low back pain score was measured in different positions. The before treatment value was 36 (median ẋ = 3.5) and the score was 72, and after treatment value was 2 (median ẋ = 0) and the score was 4. The Mann Whitney U test was performed. The p was 0.00009 (&lt;0.05).&#x0D; Conclusion: It is concluded that cod liver oil can be successfully used as a representative of vasa Sneha, and it is very effective in relieving chronic low back pain in lumbar spondylosis along with other ayurvedic preparations.

Effect of Dietary Lipid Sources on Feed Utilization, Growth Efficiency and Carcass Composition of Clarias gariepinus Juveniles

This study was designed to investigate plant and animal lipid that can enhance feed utilization, growth, and survival of Clarias gariepinus Juveniles. Juveniles with mean weight 32.50±0.5g were stocked randomly to seven tanks replicated thrice, with each tank having seven fish each and were fed with the prepared diets with varied lipid. 40% crude protein and isocaloric diets with digestible energy of 2,933.76Kcal were formulated and labelled Diet 1-7 with different sources of plant and animal lipids. Diet 1, 2 and 3 contained palm oil, Groundnut oil and soybean oil respectively, while diet 4, 5 and 6 contained Pork lard, Cod Liver oil and Beef tallow. Diet 7 (Control diet without any lipid source).&#x0D; The result showed that there was no significant difference (P&gt;0.05) in Feed conversion ratio (FCR) and survival rates among the diets. The mean weight gain was highest in fish fed with pork lard (22.12g) while the lowest value of 15.36g was recorded in fish fed with soybean oil. Relative weight gains and feed intake showed no significant difference (P&gt;0.05) between fish fed lipids of plant origin. However, there was significant difference (P&lt;0.05) among fish fed lipids from animal origin. The Highest Total Cholesterol (28.18 mg/dl) was recorded in fish fed beef tallow as lipid sources while the least value of low-density cholesterol (value of 2.26 mg/dl) was recorded in fish fed with soybean oil. In conclusion, C. gariepinus was able to utilize animal-based lipid sources more in terms of growth and feed utilization while the lipid profile result of the individual carcass after the culture period showed that C. gariepinus fed lipid from plant sources had a slightly better cholesterol level than those fed lipid of animal sources.