Coccolithus Pelagicus Research Articles

Northward advection of Atlantic water in the eastern Nordic Seas over the last 3000 yr

Abstract. Three marine sediment cores distributed along the Norwegian (MD95-2011), Barents Sea (JM09-KA11-GC), and Svalbard (HH11-134-BC) continental margins have been investigated in order to reconstruct changes in the poleward flow of Atlantic waters (AW) and in the nature of upper surface water masses within the eastern Nordic Seas over the last 3000 yr. These reconstructions are based on a limited set of coccolith proxies: the abundance ratio between Emiliania huxleyi and Coccolithus pelagicus, an index of Atlantic vs. Polar/Arctic surface water masses; and Gephyrocapsa muellerae, a drifted coccolith species from the temperate North Atlantic, whose abundance changes are related to variations in the strength of the North Atlantic Current. The entire investigated area, from 66 to 77° N, was affected by an overall increase in AW flow from 3000 cal yr BP (before present) to the present. The long-term modulation of westerlies' strength and location, which are essentially driven by the dominant mode of the North Atlantic Oscillation (NAO), is thought to explain the observed dynamics of poleward AW flow. The same mechanism also reconciles the recorded opposite zonal shifts in the location of the Arctic front between the area off western Norway and the western Barents Sea–eastern Fram Strait region. The Little Ice Age (LIA) was governed by deteriorating conditions, with Arctic/Polar waters dominating in the surface off western Svalbard and western Barents Sea, possibly associated with both severe sea ice conditions and a strongly reduced AW strength. A sudden short pulse of resumed high WSC (West Spitsbergen Current) flow interrupted this cold spell in eastern Fram Strait from 330 to 410 cal yr BP. Our dataset not only confirms the high amplitude warming of surface waters at the turn of the 19th century off western Svalbard, it also shows that such a warming was primarily induced by an excess flow of AW which stands as unprecedented over the last 3000 yr.

Abrupt variability of the last 24 ka BP recorded by coccolithophore assemblages off the Iberian Margin (core MD03‐2699)

ABSTRACTA high‐resolution coccolithophore study allowed reconstructing sea surface conditions during the last 24 ka BP off the Iberian Margin. Variations in nannoplankton accumulation rate, cold‐water species, Florisphaera profunda, Umbilicosphaera sibogae and Coccolithus pelagicus ssp. azorinus trends suggest the occurrence of substantial changes in surface water dynamics. Palaeoproductivity shows changes during the glacial and the interglacial transition from intense glacial upwelling to persistent interglacial stratification. A drastic productivity decline occurred between ∼18 and 13 ka BP related to the arrival of subpolar waters indicated by the presence of the subarctic subspecies C. pelagicus pelagicus and increased percentages of tetra‐unsaturated alkenones. Abrupt variability at core MD03‐2699 during the last 24 ka BP was observed in palaeoproductivity and sea surface temperatures proxies. Increases of reworked species fluxes were observed during the Last Glacial Maximum and during the transition to the Holocene, likely connected to turbidity currents and/or contourites formed by Mediterranean outflow intensification. In addition, C. pelagicus ssp. pelagicus presence, Gephyrocapsa muellerae and tetra‐alkenone percentages reveal rapid coolings coeval with the Holocene Bond cycles and rapid climate coolings recognized for the Northern Hemisphere between 11.02 and 1.7 ka BP and associated with subpolar water arrival at the site. Copyright © 2013 John Wiley & Sons, Ltd.

Micropaleontological record of Holocene estuarine stages in the Bahía Blanca estuary, Argentina

Holocene paleoenvironmental changes have been interpreted on the basis of benthic foraminifera and calcareous nannofossils recovered in samples from Napostá Grande Stream, Bahía Blanca estuary, southern Buenos Aires Province. Samples are fine sands and clay sediments from a Holocene outcrop and were studied with quantitative techniques. The benthic foraminiferal assemblage is dominated by Ammonia parkinsoniana, Ammonia tepida, Bolivina pseudoplicata, Bolivina striatula, Bolivina sp., Buccella peruviana, and Elphidium spp. The calcareous nannofossil assemblage recovered is a typical cold-water association, dominated by Calcidiscus leptoporus, Coccolithus pelagicus, Emiliana huxleyi and Gephyrocapsa oceanica. A dendrogram classification by cluster analysis was made for each microfossil group. The results of these analyses were coincident, showing a liaison between changes in the assemblages of benthic foraminifera and calcareous nannofossils. Those results, jointly with the sedimentological information, lead to the identification of three different paleoenvironments along the Napostá N1 site. The lower part of the succession represents an estuarine environment with larger marine connection. The middle part represents a gradual passage to a more restricted estuarine environment, and the upper part represents the establishment of the modern continental fresh-water environment.

Calcareous plankton bio-chronostratigraphy of the Maltese Lower Globigerina Limestone member

Abstract Baldassini, N., Mazzei, R., Foresi L.M., Riforgiato, F. and Salvatorini, G. 2012. Calcareous plankton bio-chronostratigraphy of the Maltese Lower Globigerina Limestone member. Acta Geologica Polonica, 63 (1), 105-135. Warszawa. The planktonic foraminifera and calcareous nannofossil biostratigraphy of the Maltese Lower Globigerina Limestone member has been investigated. The member was dated to early planktonic foraminiferal P22 Zone and nannofossil NP25 Zone (upper Chattian). A climate-stratigraphic approach, based on the quantitative analyses of calcareous nannofossils, was used additionally to achieve a more precise chronology. The species Coccolithus pelagicus (diameter £11 μm) and the genus Umbilicosphaera were selected for the recognition of cold and warm surface waters intervals respectively. The ratio of their percentages enabled the construction of a Climatic Factor (CLF) curve. The CLF values were consistent with a warm climatic phase, which is probably represented by the portion of the oxygen stable isotope curve of Miller et al. above the Oi2c event and below the beginning of the cooling trend that culminates in the Mi1 event. Considering these two climatic events and the upper boundary of the NP25 Zone, it can be inferred that the deposition of the Lower Globigerina Limestone member took place between 25.1 and 24.3 Ma.

Calcareous nannofossil assemblage changes and stable isotope data from Maastrichtian to Selandian in the Akveren Formation, Western Black Sea, Turkey

This study is based on calcerous nannofossil assemblages changes and fluctutions of stable carbon and oxygen isotopes was collected clayey limestones, limestones, and marls in the Maastrichtian to Selandian from Akveren Formation (Western Black Sea). As the relative abundances of species of Micula spp, Watznauera barnesiae, and Arkhangelskiella cymbiformis, which tolerated changes of temperature and nutrition, carbon and oxygen isotopes compositions, and low species richness imply strong diagenesis effect at the Maastrichtian, there is no important diagenesis effect at Paleocene. Just after the Cretaceous/Tertiary (K-Pg) boundary, Thoracosphaera spp. and Braarudosphaera bigelowi were dominant species; Danian is characterized by Thoracosphaera, Ericsonia ovalis, Cruciplacolithus spp., Coccolithus pelagicus, and Ericsonia subpertusa. Generally, the nutrition productivity is good–moderate in Lower Maastrichtian, and decreasing carbon isotope values during the Uppermost Maastrichtian shows the presence of oligotrophic environmental conditions suitable with global nutrition crises before the K-Pg boundary and diagenesis in study area. Throughout the Danian, mesotrophic–oligotrophic environmental conditions dominate; however, the decrease in nutrition before Selandian represents oligotrophic environmental conditions. The increasing nutrition at Selandian is related to the change in the environmental conditions.

Species-specific growth response of coccolithophores to Palaeocene–Eocene environmental change

Coccolithophores—single-celled calcifying phytoplankton—represent an essential footing to marine ecosystems, yet their sensitivity to environmental change, and in particular increases in atmospheric CO2, is poorly understood1. During the Palaeocene–Eocene Thermal Maximum (PETM), about 56 million years ago, atmospheric CO2 concentrations rose rapidly and the oceans acidified2, 3, making this an ideal time interval to examine coccolithophore responses to environmental change. Here we compare the results of experiments on modern coccolithophore species with exceptional fossil coccosphere records of the PETM, providing a cellular-level perspective. In modern taxa, we find that during the exponential growth phase of rapid cell division, small cells with few coccoliths are produced, whereas larger cells with more coccoliths are produced during slowed cell division. Applying these diagnostic features to the PETM fossil record, we find that the dominant species exhibited different growth responses to the environmental forcing. Toweius pertusus shows geometry indicative of rapid cell division. In contrast, we suggest that cells of Coccolithus pelagicus grew more slowly during the period of environmental change. In the modern ocean, Emiliania huxleyi, which is closely related to the extinct T. pertusus, is prolific and widespread, whereas C. pelagicus is more limited in range and abundance. We argue that these different responses to environmental change were critical to the post-PETM evolutionary success of the descendants of these taxa.

Fluctuations of sea water temperature based on nannofloral changes during the Middle to Late Miocene, Adana Basin, Turkey

Some nannoplankton species are sensitive to water temperatures. While Coccolithus pelagicus and Reticulofenestra gelida indicate cooler water conditions, the genera Discoaster and Sphenolithus and Calcidiscus leptoporus are indicative of warmer water environments. This paper focuses on relative fluctuation of sea water temperatures during the Middle and Late Miocene, emphasised by cold and warm nannofossil changes in abundance in 2 wells. At the A-1 well in the Middle Miocene, the total abundance of cooler water species is 45%, while that of the warmer species is 3%. During the Late Miocene, the total abundance for cooler water species decreases to 34%; in contrast, the total abundance of warmer species increases up to 7%. Thus, the cooler sea water temperature during the Middle Miocene becomes warmer in the Late Miocene. From the A-2 well, the total abundance of Middle Miocene cooler water species is 46%, but that of the warmer species is 11%. The total abundance of cooler water species decreases to 41%, and the total abundance of warmer species increases to 18% in the Late Miocene. Based on nannofloral fluctuation, we may thus deduce that water surface temperature increased from the Middle to the Late Miocene. Data on nannofossil abundance from the Miocene Adana Basin show that sea water temperature was cooler in the Middle Miocene, and water temperatures increased in the Late Miocene.

Reconstructing calcification in ancient coccolithophores: Individual coccolith weight and morphology of Coccolithus pelagicus (sensu lato)

We have adapted an existing method to estimate coccolith calcite weight using birefringence (Beaufort, 2005) to suit the large coccoliths of Coccolithus pelagicus, which are only partially birefringent under cross-polarised light microscopy. Fossil and sediment trap material from the South Tasman Rise region of the Southern Ocean was used for calibration and validation. Our approach was tested with only the coccolith central area (CA) considered for measurement, to avoid relying on the less robust proximal shields. Thus our results are relative and intend to quantify intra-specific variations in volumetric calcite weight, expressed as a Weight Index (WI). Our results were overall consistent with mass estimation based on distal shield lengths. However, the WI approach clearly has the advantage in exploring allometric scaling between coccolith size and weight, as well as in measuring the degree of calcification in similarly sized morphotypes. Combining WI and morphometry data (distal shield length, DSL), we demonstrate subtle, but statistically significant changes in shape and thus calcification degree both within and between the tested Coccolithus populations. Most strikingly, it appears that modern Coccolithus populations in the Southern Ocean are, on average, more heavily calcified than their fossil counterparts.

A Middle Pleistocene Northeast Atlantic coccolithophore record: Paleoclimatology and paleoproductivity aspects

Changes in paleoclimate and paleoproductivity patterns have been identified by analysing, in conjunction with other available proxy data, the coccolithophore assemblages from core MD03-2699, located in the Portuguese margin in the time interval from the Marine Isotope Stage (MIS) 13/14 boundary to MIS 9 (535 to 300 ka). During the Mid-Brunhes event, the assemblages associated with the eccentricity minima are characterised by higher nannoplankton accumulation rate (NAR) values and by the blooming of the opportunistic genus Gephyrocapsa. Changes in coccolithophore abundance are also related to glacial–interglacial cycles. Higher NAR and numbers of coccoliths/g mainly occurred during the interglacial periods, while these values decreased during the glacial periods. Superimposed on the glacial/interglacial cycles, climatic and paleoceanographic variability has been observed on precessional timescales. The structure of the assemblages highlights the prevailing long-term influence of the Portugal (PC) and Iberian Poleward (IPC) Currents, following half and full precession harmonics, related to the migration of the Azores High (AH) Pressure System. Small Gephyrocapsa and Coccolithus pelagicus braarudii are regarded as good indicators for periods of prevailing PC influence. Gephyrocapsa caribbeanica, Syracosphaera spp., Rhabdosphaera spp. and Umbilicosphaera sibogae denote periods of IPC influence. Our data also highlights the increased percentages of Coccolithus pelagicus pelagicus during the occurrence of episodes of very cold and low salinity surface water, probably related to abrupt climatic events and millennial-scale oscillations of the AH/Icelandic Low (IL) System.

Monitoring fluctuations of the Subtropical Front in the Tasman Sea between 3.45 and 2.45 Ma (ODP site 1172)

Calcareous nannoplankton assemblages and benthic δ 18O isotopes of Pliocene deep-sea sediments of ODP site 1172 (East of Tasmania) have been studied to improve our knowledge of the Southern Ocean paleoceanography. Our study site is located just north of the Subtropical Front (STF), an ideal setting to monitor migrations of the STF during our study period, between 3.45 and 2.45 Ma. The assemblage identified at ODP site 1172 has been interpreted as characteristic for the transitional zone water mass, located south of the STF, based on: (i) the low abundances (< 1%) of subtropical taxa, (ii) relatively high percentages of Coccolithus pelagicus, a subpolar type species, (iii) abundances from 2-10% of Calcidiscus leptoporus, a species that frequently inhabits the zone south of the STF and (iv) the high abundances of small Noelaerhabdaceae which at present dominates the zone south of the STF. Across our interval the calcareous nannoplankton manifests glacial-interglacial variability. We have identified cold events, characterized by high abundances of C. pelagicus which coincide with glacial periods, except during G7. After 3.1 Ma cold events are more frequent, in concordance with global cooling trends. Around 2.75 Ma, the interglacial stage G7 is characterized by anomalous low temperatures which most likely are linked to definite closure of the Central American Seaway (CAS), an event that is believed to have had global consequences. A gradual increase of very small Reticulofenestra across our section marks a significant trend in the small Noelaerhabdaceae species group and has been linked to a general enhanced mixing of the water column in agreement with previous studies. It is suggested that a rapid decline of small Gephyrocapsa after isotopic stage G7 might be related to the cooling observed in our study site after the closure of the CAS.

A voltage-gated H+ channel underlying pH homeostasis in calcifying coccolithophores.

Marine coccolithophorid phytoplankton are major producers of biogenic calcite, playing a significant role in the global carbon cycle. Predicting the impacts of ocean acidification on coccolithophore calcification has received much recent attention and requires improved knowledge of cellular calcification mechanisms. Uniquely amongst calcifying organisms, coccolithophores produce calcified scales (coccoliths) in an intracellular compartment and secrete them to the cell surface, requiring large transcellular ionic fluxes to support calcification. In particular, intracellular calcite precipitation using HCO3 − as the substrate generates equimolar quantities of H+ that must be rapidly removed to prevent cytoplasmic acidification. We have used electrophysiological approaches to identify a plasma membrane voltage-gated H+ conductance in Coccolithus pelagicus ssp braarudii with remarkably similar biophysical and functional properties to those found in metazoans. We show that both C. pelagicus and Emiliania huxleyi possess homologues of metazoan Hv1 H+ channels, which function as voltage-gated H+ channels when expressed in heterologous systems. Homologues of the coccolithophore H+ channels were also identified in a diversity of eukaryotes, suggesting a wide range of cellular roles for the Hv1 class of proteins. Using single cell imaging, we demonstrate that the coccolithophore H+ conductance mediates rapid H+ efflux and plays an important role in pH homeostasis in calcifying cells. The results demonstrate a novel cellular role for voltage gated H+ channels and provide mechanistic insight into biomineralisation by establishing a direct link between pH homeostasis and calcification. As the coccolithophore H+ conductance is dependent on the trans-membrane H+ electrochemical gradient, this mechanism will be directly impacted by, and may underlie adaptation to, ocean acidification. The presence of this H+ efflux pathway suggests that there is no obligate use of H+ derived from calcification for intracellular CO2 generation. Furthermore, the presence of Hv1 class ion channels in a wide range of extant eukaryote groups indicates they evolved in an early common ancestor.

Calcareous nannofossil changes during the Mid-Pleistocene Revolution: Paleoecologic and paleoceanographic evidence from North Atlantic Site 980/981

New quantitative data on calcareous nannofossil assemblages through 583–1138 thousand years ago (kyr) have been acquired in high sample resolution (2–3ky) at northern Atlantic ODP (Ocean Drilling Program) Site 980/981. Assemblage composition reflects a temperate paleobioprovince suggesting significant influx of warm North Atlantic Current at the site location during the mid-Pleistocene transition, between Marine Isotope Stage (MIS) 35 and 15.Three intervals have been described based on changes in nannofossil composition and abundance fluctuations of taxa. Reticulofenestrids are abundant just in the first interval (MIS 35–22), which is also characterised by the absence of Gephyrocapsa (>4μm) and C. pelagicus s.l., the latter occurring in upper MIS 24 only. The second interval corresponds to the expanded warm MIS 21 that is accompanied by high carbonate content, absence of both nannofossil reworking and ice rafted debris (IRD), higher abundance of C. leptoporus (5–8μm) and H. carteri, and absence of C. pelagicus s.l. and medium gephyrocapsids. The third interval (MIS 20–15) records the occurrence of C. pelagicus s.l. and medium Gephyrocapsa, generally higher abundance and higher amplitude fluctuations of helicoliths, and absence of reticulofenestrids. The major changes in nannofossil assemblages seem to be the result of combined paleoenvironmental factors related to sea surface water features (temperature and nutrient availability, sea-ice cover) and possibly to deep and intermediate water production of both Northern and Southern Hemisphere origin during glacial and interglacial phases. Complex inter-relationships between environmental parameters and evolutionary/ecological factors are inferred to explain the patterns of Coccolithus pelagicus subspecies, whose distribution and abundance fluctuations are compared with the available patterns of Turborotalia quinqueloba and Neogloboquadrina pachyderma (left coiled) at Site 980 and with the C. pelagicus subspecies patterns from North Atlantic DSDP (Deep Sea Drilling Project) Site 607 and South Atlantic ODP Site 1090.Data collected provide additional information on stratigraphic distribution of selected taxa; the First Occurrence (FO) of Gephyrocapsa omega is confirmed as a diachronous event, even in the Atlantic Ocean, while the First and Last Common Occurrence (FCO, LCO) of R. asanoi appear as diachronous and synchronous events, respectively, on a global scale.

Perturbing phytoplankton: response and isotopic fractionation with changing carbonate chemistry in two coccolithophore species

Abstract. All species of coccolithophore appear to respond to perturbations of carbonate chemistry in a different way. Here, we show that the degree of malformation, growth rate and stable isotopic composition of organic matter and carbonate produced by two contrasting species of coccolithophore (Gephyrocapsa oceanica and Coccolithus pelagicus ssp. braarudii) are indicative of differences between their photosynthetic and calcification response to changing DIC levels (ranging from ~1100 to ~7800 μmol kg−1) at constant pH (8.13 ± 0.02). Gephyrocapsa oceanica thrived under all conditions of DIC, showing evidence of increased growth rates at higher DIC, but C. braarudii was detrimentally affected at high DIC showing signs of malformation, and decreased growth rates. The carbon isotopic fractionation into organic matter and the coccoliths suggests that C. braarudii utilises a common internal pool of carbon for calcification and photosynthesis but G. oceanica relies on independent supplies for each process. All coccolithophores appear to utilize bicarbonate as their ultimate source of carbon for calcification resulting in the release of a proton. But, we suggest that this proton can be harnessed to enhance the supply of CO2(aq) for photosynthesis either from a large internal HCO3- pool which acts as a pH buffer (C. braarudii), or pumped externally to aid the diffusive supply of CO2 across the membrane from the abundant HCO3- (G. oceanica), likely mediated by an internal and external carbonic anhydrase respectively. Our simplified hypothetical spectrum of physiologies may provide a context to understand different species response to changing pH and DIC, the species-specific εp and calcite "vital effects", as well as accounting for geological trends in coccolithophore cell size.

Surface water dynamics in the Reykjanes Ridge area during the Holocene as revealed by coccolith assemblages

The calcareous nannofossil assemblages from sediment core DS97-2P from the Reykjanes Ridge have been investigated to document oceanographic changes in surface water during the Holocene. The recorded variations in coccolithophore species assemblages and accumulation rates indicate that the region was subjected to rapid changes of surface water masses throughout the entire Holocene. Coccolithophore assemblages generally are of low species diversity and consist mainly of Emiliania huxleyi and Coccolithus pelagicus ssp. pelagicus. Two major events occurred at 8.5–7 ka and at 4.5– 3.5 ka, showing higher coccolith accumulation rates, suggesting that the influence of relatively warm Atlantic waters via the Irminger Current was strong in the investigated area. The coccolithophore assemblages have been compared with diatom, foraminifer and sedimentological records within the same core. These data, supported by a comparison with previously published proxy records, add credit to the hypothesis that Holocene changes did not occur uniformly across the North Atlantic. The results have highlighted the Holocene pattern in the North Atlantic, as a period influenced by strong regionalism with discrepancies in the hydrographical trends and in the distribution of the planktonic proxies.

Strontium/Calcium ratio, carbon and oxygen stable isotopes in coccolith carbonate from different grain-size fractions in South Atlantic surface sediments

In this study, the Strontium/Calcium (Sr/Ca) ratio, and the carbon and oxygen isotopic compositions of coccoliths are investigated in three different grain-size fractions (<20 μm, 15–5 μm, <5 μm) of 17 surface sediment samples from the Equatorial and South Atlantic. The results are compared to environmental parameters in order to assess the factors controlling the observed coccolith geochemical patterns. Isotopic and geochemical composition of coccolith species in surface sediment samples from the South Atlantic greatly varies according to the different grain-size fractions. However, even if the absolute values show a great offset, the general trends are comparable. The δ 18O values show a decreasing trend with increasing temperature. The δ 13C and Sr/Ca ratio are mainly influenced by productivity of coccolithophores, which is in turn controlled by different factors, such as temperature, nutrient supply and productivity of other phytoplankton groups. Dilution and dissolution are negligible factors in these open marine samples. Therefore, coccolith abundance in bulk sediment is the best approximation for productivity of coccolithophores. The various coccolith species fractionate Sr differently, as is best shown by the 5–15 μm fraction where three species ( Calcidiscus leptoporus, Helicosphaera carteri and Coccolithus pelagicus) predominantly occur.

Coccolith distribution patterns in surface sediments of Equatorial and Southeastern Pacific Ocean

This study aims to contribute to a more detailed knowledge of the biogeography of coccolithophores in the Equatorial and Southeastern Pacific Ocean. Census data of fossil coccoliths are presented in a suite of core-top sediment samples from 15°N to 50.6°S and from 71°W to 93°W. Following standard preparation of smear slides, a total of 19 taxa are recognized in light microscopy and their relative abundances are determined for 134 surface sediment samples. Considering the multivariate character of oceanic conditions and their effects on phytoplankton, a Factor Analysis was performed and three factors were retained. Factor 1, dominated by Florisphaera profunda and Gephyrocapsa oceanica, includes samples located under warm water masses and indicates the occurrence of calcite dissolution in the water column in the area offshore Chile. Factor 2 is related to cold, low-salinity surface-water masses from the Chilean upwelling, and is dominated by Emiliania huxleyi, Gephyrocapsa sp. < 3 μm, Coccolithus pelagicus and Gephyrocapsa muellerae. Factor 3 is linked to more saline, coastal upwelling areas where Calcidiscus leptoporus and Helicosphaera carteri are the dominant species.

Response of calcareous nannofossil assemblages to paleoenvironmental changes through the mid-Pleistocene revolution at Site 1090 (Southern Ocean)

Quantitative study on calcareous nannofossil assemblages has been performed in high time resolution (2–3 kyr) at the Ocean Drilling Program Site 1090. The location of this site in the Southern Ocean is crucial for the comprehension of thermohaline circulation and frontal boundary dynamics, and for testing the employ of nannoflora as paleoceanographical tool. The chronologically well constrained investigated record spans between Marine Isotope Stage (MIS) 35 and 15, through an interval of global paleoclimate and paleoceanographical modification also known as mid-Pleistocene revolution (MPR). Measures of ecological (Shannon–Weaver diversity and paleoproductivity) and dissolution indices together with spectral and wavelet analyses carried out on the acquired time series provide valuable information for interpretation of data in terms of paleoecology and paleoceanography. Assemblages are mainly represented by dominant small Gephyrocapsa, common Calcidiscus leptoporus s.l., Coccolithus pelagicus s.l., Gephyrocapsa (4-5.5 μm), the extinct Pseudoemiliania lacunosa and Reticulofenestra spp. ( R. asanoi and Reticulofenestra sp.). Morphotypes discriminated within Calcidiscus leptoporus s.l. and Coccolithus pelagicus s.l., reveal that they may have had different ecological preferences during Pleistocene with respect to the present. The composition and fluctuation in nannofossil assemblage and their comparison with the available Sea Surface Temperature (SST) and C-org curves suggest a primary ecological response to paleoenvironmental changes; relationships to different surface water features and boundary dynamics, as well as to different efficiencies and motions of the intermediate and deep water masses have been inferred. A more northward position of Subantarctic Front (SAF) during most of the Early Pleistocene record has been highlighted based on assemblage composition characterised by common Calcidiscus leptoporus s.l., Coccolithus pelagicus s.l., medium Gephyrocapsa (4–5.5 μm), and by the rarity or absence of Umbilicosphaera spp., Rhabdosphaera spp., Pontosphaera spp., Oolithotus fragilis. Exceptions are the more intense interglacials MIS 31, 17, and probably MIS 15, when a southward displacement of frontal system occurred, coincident with peaks in abundance of Helicosphaera spp. and Syracosphaera spp. Higher nutrient content and more dynamic conditions occurred between MIS 32 and MIS 25, in relation to shallower location of nutrient-rich Antarctic Intermediate Water (AAIW) core and to reduction of glacial–interglacial variability. A nannofossil barren interval is coincident with the known stagnation of South Atlantic deep water circulation during MIS 24, when North Atlantic Deep Water (NADW) was reduced or suppressed and an enhanced northward deep penetration of the more corrosive Circumpolar Deep Water (CPDW) took place. An event of strong instability in nutricline dynamics characterised the transition MIS 23–22 as suggested by sharp fluctuations in paleoproductivity proxies, linked to major changes in oceanographic circulation and to the first distinct increase of larger ice volumes at this time. From MIS 21 upward the nannofossil variations seem to be primarily controlled by glacial–interglacial cyclicity and temperature fluctuations. The cyclic fluctuation recognised in nannofossil abundance seems to be linked to orbitally-forced climatic variation, primarily to the obliquity periodicity recorded in the patterns of C. leptoporus intermediate (5–8 μm) and C. pelagicus pelagicus (6–10 μm); however no obvious and linear relations may be always observed between nannoflora fluctuation and Milankovitch parameters, suggesting more complex and unclear relationships between nannofossils and environmental change.

Cases of shell disease in the giant oyster Crassostrea gigas (Bivalvia) cultivated in the Black Sea

This paper presents the first description of shell disease in the Crassostrea gigas oyster cultivated in the Black Sea. The shell margin, hinge and the site of adductor muscle attachment are affected by a fungus. The oyster’s exhaustion caused by excessive sedimentation results in high susceptibility to disease. It is suggested that spores of the fungus Ostracoblabe implexa were found in the silt. The disease was discovered in oysters that were lacking growth zones or that had died. Microphotos show the fungus’s ramified hyphae, 1.5–3 μm in diameter, with nodes and septa located in the fragments of affected oyster nacre matrix. They also show the close contact between two hyphae and the presence of “suckers” spreading from the mycelium to the bells of aragonite. We discuss the issue of a temporary symbiosis between the fungus and the microalga Coccolithus pelagicus, which could explain the adaptability of the fungus and the fact that its life cycle continues after the death of infected oysters.

Coccolithophores in the upwelling waters of Portugal: Four years of weekly distribution in Lisbon bay

From July 2001 to May 2005, seawater samples were collected once a week at a fixed station in Lisbon bay (38°41′N, 09°24′W) in order to describe the ecological dynamics of the coccolithophore community. The seasonal and interannual distribution patterns of the different species and their relationships with environmental parameters are addressed. The present work aimed to identify potential proxies for different local water bodies and environmental conditions. Throughout the period of study, the upwelling events were weak and progressively more persistent. High sea surface temperatures (SST) were observed earlier in the year; summers and winters were gradually warmer and colder, respectively. Salinity variations reflected the different weather conditions as they are strongly influenced by rainfall and thus by the Tagus river flow. The extended periods of weak upwelling and the overall increase in SST resulted in the development of phytoplankton populations as measured by chlorophyll a. However, the persistence of the upwelling, and thus shorter convergence periods, favoured other phytoplankton groups than coccolithophore populations as these decreased towards the end of the sampling period. The annual structure of the coccolithophore assemblage showed a pronounced and recurrent seasonal variability, mainly related with the intensity and persistence of upwelling. The highest cell densities were recorded from spring to autumn. An overall preference by most species for mature upwelled waters and low turbulent conditions was observed associated with high temperatures and salinities, although the species develop in different windows with mismatching maxima. The coccolithophores observed were capable of withstanding coastal processes such as turbulence and were well adapted to an environment rich in nutrients provided by both continental runoff and upwelling. The consistency of the results enabled local oceanographic tracers to be defined. Emiliania huxleyi and Gephyrocapsa species can be used as proxies of surface productivity waters during spring and summer while Coccolithus pelagicus indicates the presence of upwelling fronts. Calcidiscus leptoporus is a tracer of the convergence of subtropical oceanic waters onto the shelf, during winter while Coronosphaera mediterranea, Syracosphaera pulchra, Helicosphaera carteri and Rhabdosphaera clavigera revealed the presence of those waters during the short period that characterized the transition from upwelling to downwelling seasons.

Coccolithophore size rules — Reconstructing ancient cell geometry and cellular calcite quota from fossil coccoliths

Algal cell size exerts a fundamental influence on the stable isotopic fractionation during photosynthesis and biocalcification, as well as the incorporation of trace elements in coccolith calcite by calcifying haptophytes (the coccolithophores). Therefore, it is important to evaluate the influence of ancient coccolithophore cell size on coccolithophorid-derived geochemical proxies that are applied in paleoceanographic studies. However, in contrast to planktonic foraminifera, for which cell size may be directly estimated from their fossilized calcite shells, the reconstruction of ancient coccolithophore cell dimensions appears hampered because the sediment record is dominated by single coccoliths, rather than intact coccospheres — the armor of multiple interlocking calcite platelets that surround live cells. Solving this apparent problem, this paper presents quantitative constraints on coccolith size, coccosphere and cell diameters, for three main Cenozoic genera of coccolithophore ( Reticulofenestra, Cyclicargolithus and Coccolithus) through detailed biometry of rarely fossilized coccospheres of middle Eocene to early Miocene age. Together, these taxa are most dominant in Cenozoic deep-sea sediments, providing an exquisitely detailed record of their evolution and the bulk of biogenic carbonate burial through time. Accurate estimates of coccolithophore cell size can be made by relatively simple size measurements of individual coccoliths in fossil assemblages, since coccolith size is strongly linearly correlated to both coccosphere- and cell diameter. Additional general coccolith ‘size rules’ are confirmed by the morphometric data: (1) coccolith thickness increases with size, (2) elliptical placoliths are more circular at larger size, and (3) the number of coccoliths per coccosphere is rather invariable within the investigated Cenozoic genera. The latter observation offers better control on (minimum) estimates of cellular calcite quota in the ancestors of today's prolific bloom-forming species Emiliania huxleyi, Gephyrocapsa spp. and Coccolithus pelagicus.