Coastal Wetland Sediments Research Articles

Contribution of Microplastics to Carbon Storage in Coastal Wetland Sediments

The ubiquitous occurrence of microplastics in coastal habitats is widely reported. As an anthropogenic material with high carbon content, the contribution of microplastics to the coastal sediment carbon stock is still unassessed. Here, we report the occurrence pattern of microplastics (20-2000 μm) and estimate their contribution to the carbon stock in different coastal sediments in Hong Kong, a metropolis in the Pearl River estuary and part of the Greater Bay Area with a population of 70 million. Our data reveal that microplastics are more concentrated in the surface sediment and in vegetated coastal sediments. We also estimate that at present carbon from microplastics contributes between 0.001% and 1.197% to the sediment carbon pool at different depths. Despite the low contribution, microplastics have already made a hidden contribution to the coastal sediment carbon stock. Our data help to establish a baseline for future contributions of microplastics to total carbon storage in peri-urban coastal sediments.

Mercury enrichment in anthrosols and adjacent coastal sediments at a Classic Maya site, Marco Gonzalez, Belize

AbstractElevated concentrations of total mercury (THg) are found in the surface soils and flanking wetland sediments at the Classic Maya coastal site of Marco Gonzalez, Belize. Significant concentrations (up to 1.3 µg·g−1dry mass) of THg occur in leaf litter‐rich soils, as well as in the artefact‐rich anthrosol spread over the vegetated mound site of structures and occupation debris. The abundance and spatial pattern of major and trace elements measured in the surface soils indicate both site‐scale controlling factors of topography, structures and vegetation on soil geochemistry as well as local highs in concentration compared with background, due to human activity. Geochemical stratigraphy of wetland sediment cores shows that a shift from carbonate‐reef sediments to mangrove peat in the 13th century AD was attended by an input of allogenic (mineral) elements, including mercury. A THg concentration peak (0.8 μg·g−1) in brackish pool sediment is210Pb‐dated to 1960–1970 AD, but the incorporation of mercury in multiple cores adjacent to the site shows increasing mercury inputs to have occurred before, during Classic‐period Maya occupation and following the sites abandonment. Analysis of element values from site‐scale soil sampling, combined with results from off‐site cores, provides a numerical framework upon which outlier values of THg and other element spatial patterns can be assessed, especially the spatial co‐association of elements related to differences in soil–sediment matrices. Geochemical results from active soils developing from occupation deposits (anthrosols) and sediment cores open up questions concerning contemporary and past mercury accumulation at coastal Mayan sites, and the wider interaction of human and natural biogeochemical processes that occur in human‐modified soils and coastal wetland sediments.

Deciphering cryptic methane cycling: Coupling of methylotrophic methanogenesis and anaerobic oxidation of methane in hypersaline coastal wetland sediment

Methanogenesis has recently been shown to fuel anaerobic oxidation of methane (AOM) within the sulfate-reducing zone of marine sediments, coining the term “cryptic methane cycle”. Here we present research on the relationship between methanogenesis and AOM in a shallow hypersaline pool (∼130 PSU) within a southern California coastal wetland. Sediment (top 20 cm) was subjected to geochemical analyses, in-vitro slurry experiments, and radiotracer incubations using 35S-SO42−, 14C-mono-methylamine, and 14C-CH4, to study sulfate reduction, methylotrophic methanogenesis, and AOM. An adapted radioisotope method was used to follow cryptic methane cycling in 14C-mono-methylamine labeling incubations with increasing incubation times (1 hour to three weeks). Results showed peaks in AOM (max 13 nmol cm−3 d−1) and sulfate reduction activity (max 728 nmol cm−3 d−1) within the top 6 cm. Below 6 cm, AOM activity continued (max 15 nmol cm−3 d−1), while sulfate reduction was absent despite 67 mM sulfate, suggesting AOM was coupled to the reduction of iron. Methane concentrations were low (<50 nM) throughout the sediment. Batch sediment slurry incubations with methylated substrates (mono-methylamine and methanol) stimulated methanogenesis, pointing to the presence of methylotrophic methanogens. Incubations with 14C-mono-methylamine revealed the simultaneous activity of methanogenesis and coupled AOM through the stepwise transfer of 14C from mono-methylamine to CO2 via methane. Our results suggest that AOM is a crucial process in coastal wetland sediments to prevent the buildup of methane in the sulfate-reducing zone. We propose that cryptic methane cycling has been largely overlooked in coastal wetlands resulting in incomplete understanding of carbon cycling in this environment.

Onset of runaway fragmentation of salt marshes

Summary Salt marshes are valuable but vulnerable coastal ecosystems that adapt to relative sea level rise (RSLR) by accumulating organic matter and inorganic sediment. The natural limit of these processes defines a threshold rate of RSLR beyond which marshes drown, resulting in ponding and conversion to open waters. We develop a simplified formulation for sediment transport across marshes to show that pond formation leads to runaway marsh fragmentation, a process characterized by a self-similar hierarchy of pond sizes with power-law distributions. We find the threshold for marsh fragmentation scales primarily with tidal range and that sediment supply is only relevant where tides are sufficient to transport sediment to the marsh interior. Thus the RSLR threshold is controlled by organic accretion in microtidal marshes regardless of the suspended sediment concentration at the marsh edge. This explains the observed fragmentation of microtidal marshes and suggests a tipping point for widespread marsh loss.

Changes in Sediment Organic Carbon Accumulation under Conditions of Historical Sea-Level Rise, Southeast Saline Everglades, Florida, USA

Mangroves can store more sediment organic carbon (SOC) than freshwater and salt marshes. Understanding how mangroves have responded to historical sea-level rise (SLR) is fundamental to assessing their resilience and capacity to store carbon as SLR accelerates. We quantified landscape-level temporal and spatial trends in historical coastal wetland sediment accumulation and associated SOC content (i.e., storage) along coastal-to-inland gradients in Southeast Florida. The observed trends were transgressive and attributed to the historical rise in sea level. Our results indicate an overall significant increase in the SOC content of the historic wetland sediment succession caused by the vertical accumulation and landward migration of carbon-rich mangrove-dominated plant communities (mean = 0.08 g cm−3) into and over carbon-poor wet prairie plant communities (mean = 0.02 g cm−3). The observed historical increase in SOC is predicted to diminish over time as the difference between rates of SLR and vertical sediment accumulation increases and because the landward migration of mangrove-dominated plant communities is now obstructed by a shore-parallel flood-control levee. These results are likely to unfold in other low-latitude coastal wetlands where they are sandwiched between rising seas and an urbanized landscape.

How mangrove plants affect microplastic distribution in sediments of coastal wetlands: Case study in Shenzhen Bay, South China

Microplastic pollution is common in marine and coastal ecosystems, especially in mangrove wetlands. However, factors affecting the distribution of microplastics, such as plants, have not been sufficiently studied. We investigated the effect of different plant species on the distribution of sediment microplastics in two Nature Reserves in South China, viz. Futian Mangrove and Mai Po Mangrove. In Futian Mangrove, the abundance of total microplastics among three monospecific mangrove stands dominated by Sonneratia caseolaris, Kandelia obovata, and Sonneratia apetala was similar. The abundance of microplastics in the mudflat was similar to that in the forest interior, except for the fact that more fiber was found in the mudflat than in the interior of Sonneratia apetala. This suggested that the dense pneumatophores at the fringe prevented fibers from entering the mangrove forest. The significant positive dependence (p < 0.05) between the density of Sonneratia pneumatophores and the abundance of fibers highlighted the importance of pneumatophores. The abundance of total microplastics, predominantly in the form of fibers, in sediments at the forest fringe (2835 ± 713 items/kg d.w. and 2070 ± 224 items/kg d.w. in Futian and Mai Po, respectively) was higher than that in the forest interior and mudflat. There was no difference between the two latter locations in both mangroves, which demonstrated the significance of the fringe effect. This paper reports for the first time that the spatial distribution of microplastics in mangrove sediments was affected by plant species, which provides useful information for environmental processes of microplastics in coastal wetlands.

Exogenous nitrogen addition inhibits sulfate-mediated anaerobic oxidation of methane in estuarine coastal sediments

Anaerobic oxidation of methane (AOM) plays an important role in regulating methane (CH4) emissions from coastal wetland ecosystems. In recent decades, coastal estuarine wetlands have been subjected to great environmental pressure associated with terrestrial nitrogen (N) input. Here, we used 13CH4 to quantify AOM rates in coastal wetland sediments with and without vegetation in the Yangtze River Estuary in East China. Impact of addition of inorganic N forms (NO3−, NO2− and NH4+) on AOM process was assessed by 13CH4 anaerobic oxidation into 13C-CO2 and 13C-SOC, as well as by community structure and quantitative characteristics of anaerobic methanotrophic (ANME) archaea. The results showed that AOM process occurred in the bare wetland (BW) and reed wetland (RW). Inorganic N addition, regardless of N forms, notably decreased AOM rates by 14–58% in the BW sediments and by 26–65% in the RW sediments. Rates of 13CH4 assimilation into SOC, which were similar to but faster than rates of AOM into CO2, were reduced by 12%–66% under inorganic N addition. After 120-day anaerobic incubation, the net changes in the relative abundance of ANME-2 and ANME-3 were significantly correlated with AOM rates in the RW and BW, respectively, thereby indicating their contribution to the variation in AOM under N addition. Our findings provide insight into the potential consequences of terrestrial N input; these may weaken CH4 consumption by anaerobic oxidation in the Yangtze River Estuary, and decrease carbon stabilization and storage in coastal wetland sediments.

Organic carbon and reduced inorganic sulfur accumulation in subtropical saltmarsh sediments along a dynamic coast, Yancheng, China

Abstract The carbon and sulfur cycles in coastal wetland sediments are intricately linked. We measured total organic carbon (TOC) and total reduced inorganic sulfur (TRIS) in sulfate-rich wetland sediments at middle and low elevation saltmarshs along the Yancheng coast, China. Results show that the average TOC concentrations (5.01 ± 2.31 kg C/m3) associated with Spartina sediments in mid elevation sites is much higher than that seen in low elevation mudflats (0.65 ± 0.25 kg C/m3). TRIS concentrations in Spartina sediments ranged from 21.7 ± 11.2 μmol·cm−3 to 3.0 ± 2.0 μmol·cm−3. Within the TRIS pool, elemental sulfur (ES) constituted the lowest fraction (3.6% to 31.4%) and chromium reduced sulfur (CRS) and acid volatile sulfur (AVS) fractions were higher particularly at coasts characterized by siltation, ranging from 29.9% to 62.2% and from 28% to 59%, respectively. AVS or CRS contents correlated positively with dissolved iron/SO42− and pH, while no clear relationship was present between the TRIS fractions and the abundance of sulfate reduction genes or between the TRIS fractions and TOC. Massive pyrite was the dominant authigenic pyrite present at all sites. Our results suggest that with sea level rise, prolonged inundation and rapid siltation would increase TRIS (especially AVS) and TOC accumulation concurrently while coastal erosion and strong waves would reduce both TOC and TRIS accumulation but increase the ES proportion due to exposure to aerobic conditions.

Geochemical controls on the elemental composition of siderite: Implications for palaeo-environmental reconstructions

The elemental composition of siderite (FeCO3) has been widely used as a palaeo-environmental proxy, and commonly utilised in the diagnosis of marine influences on Phanerozoic coastal wetland sediments. That siderite might reflect marine or freshwater sources is based on the premise that a marine origin should show an enrichment of Ca and/or Mg, while high concentrations of Mn should reflect precipitation from freshwater. However, the main controls on the elemental composition of siderite, and the degree to which siderite composition reflects parent water chemistry, are poorly understood. To address these issues, we conducted siderite nucleation and seeded growth experiments to examine element partitioning at a variety of saturation states and solution compositions (i.e., cation concentrations, pH, and pCO2). Results indicate a strong preference for Mn uptake over Ca and Mg, even when Mn is present at concentrations far below those of Ca or Mg. This uptake is enhanced at slower growth rates and lower saturation states, which may, in part, reflect surface structural controls on uptake and or a change in the dominant growth mechanism. Ca uptake only occurred during nucleation experiments, while measurable Mg uptake was not observed in experiments conducted at 20 °C, but increased slightly at 55 °C. These results suggest that the chemical composition of siderite does not directly reflect aqueous cation chemistry as previously assumed and is strongly influenced by kinetics rather than equilibrium behaviour. This leads us to conclude that variations in pore water chemistry alone cannot explain the range of Ca and Mg concentrations reported from geological siderites, and that other factors must be considered (such as physical admixtures of multiple carbonate minerals and/or the products of diagenetic recrystallization).

The origins and implications of glycerol ether lipids in China coastal wetland sediments

Coastal wetlands are terrestrial-marine transition zones harboring diverse active microbial communities. The origins of diverse glycerol ether lipids preserved in coastal wetlands are rarely investigated. 16 surface sediments were collected from the coastal wetland at Guangrao (GR), Changyi (CY) and Xiamen (XM), where both climate and sedimentary environment show significant differences. Ten groups of glycerol ether lipids, including isoprenoidal and branched glycerol dialkyl glycerol tetraethers (iGDGTs and bGDGTs), isoprenoidal and branched glycerol dialkanol diethers (iGDDs and bGDDs), hydroxylated isoprenoidal GDGTs and GDDs (OH-GDGTs and OH-GDDs), overly branched GDGTs (OB-GDGTs), sparsely branched GDGTs (SB-GDGTs), hybrid isoprenoid/branched GDGTs (IB-GDGTs) and a tentatively assigned H-shaped branched GDGTs (H-B-GDGTs) were detected and quantified. Sediments collected in the north (Guangrao and Changyi) contain, in general, a lower abundance of GDGT (3.7–55.9 ng/g sed) than samples from south (Xiamen; 251–1020 ng/g sed). iGDGTs and bGDGTs are the predominant components at all sites and account for 17.2–74.3% and 16.1–75.1% of total ether lipids, respectively. The relative abundance of iGDGTs decreases but that of bGDGTs increases with the distance from sea, suggesting a marine vs. terrestrial origin of iGDGT and bGDGTs, respectively. In addition, the methylation index (MIOB/B/SB) of branched GDGTs shows a significant inverse correlation with water content, suggesting that marine waters have a major influence on the microbial communities inhabiting wetland sediment. Such an assumption was confirmed by the distinct lipid pattern of three low water content (<5%) samples collected in an area isolated from tidal flushing. The other isoprenoidal ether lipids, such as iGDDs, OH-GDGTs and OH-GDGTs, have a similar distribution as iGDGTs, indicating a common biological source, so do the corresponding non-isoprenoidal ether lipid series with bGDGTs. The BIT value increases with increasing distance from the sea, which implies that the BIT index can be probably applied to trace past sea level change in costal wetland settings. The reconstructed temperature from TEX86 shows significant offset from observed data, but only little deviation for the MBT/CBT calculated temperature. This suggests that the MBT/CBT has the potential to reconstruct past temperatures in coastal wetland settings.

Spatial Distribution Characteristics of Nitrogen and Phosphorus in Sediments of Coastal Wetlands

Spatial Distribution Characteristics of Nitrogen and Phosphorus in Sediments of Coastal Wetlands

Importance of denitrification driven by the relative abundances of microbial communities in coastal wetlands

Excessive nitrogen (N) loadings from human activities have led to increased eutrophication and associated water quality impacts in China's coastal wetlands. Denitrification accounts for significant reduction of inorganic N to nitrous oxide (N2O) or dinitrogen gas (N2), and thereby curtails harmful effects of N pollution in coastal and marine ecosystems. However, the molecular drivers and limiting steps of denitrification in coastal wetlands are not well understood. Here, we quantified the abundances of functional genes involved in N cycling and determined denitrification rates using 15N paring technique in the coastal wetland sediments of Bohai Economic Rim in eastern China. Denitrification accounting for 80.7 ± 12.6% of N removal was the dominant pathway for N removal in the coastal wetlands. In comparison, anaerobic ammonium oxidation (ANAMMOX) removed up to 36.9 ± 7.3% of inorganic N. Structural equation modeling analysis indicated that the effects of ammonium on denitrification potential were mainly mediated by the relative abundances of nosZ/nirS, nirS/(narG + napA) and amoA/nirK. Denitrification was limited by the relative strength of two steps, namely N2O reduction to N2 and nitrite (NO2−) reduction to nitric oxide (NO). Our results suggest that the relative abundances of functional genes which are more stable than sediment chemical compounds in the context of environmental changes are indictive of denitrification potential in coastal wetlands.

Community Structure, Dynamics and Interactions of Bacteria, Archaea and Fungi in Subtropical Coastal Wetland Sediments

Bacteria, archaea and fungi play crucial roles in wetland biogeochemical processes. However, little is known about their community structure, dynamics and interactions in subtropical coastal wetlands. Here, we examined communities of the three kingdoms in mangrove and mudflat sediments of a subtropical coastal wetland using Ion Torrent amplicon sequencing and co-occurrence network analysis. Bacterial, archaeal and fungal communities comprised mainly of members from the phyla Proteobacteria and Bacteroidetes, Bathyarchaeota and Euryarchaeota, and Ascomycota, respectively. Species richness and Shannon diversity were highest in bacteria, followed by archaea and were lowest in fungi. Distinct spatiotemporal patterns were observed, with bacterial and fungal communities varying, to different extent, between wet and dry seasons and between mangrove and mudflat, and archaeal community remaining relatively stable between seasons and regions. Redundancy analysis revealed temperature as the major driver of the seasonal patterns of bacterial and fungal communities but also highlighted the importance of interkingdom biotic factors in shaping the community structure of all three kingdoms. Potential ecological interactions and putative keystone taxa were identified based on co-occurrence network analysis. These findings facilitate current understanding of the microbial ecology of subtropical coastal wetlands and provide a basis for better modelling of ecological processes in this important ecosystem.

Whether hurricane Katrina impacted trace metal and dioxin depositional histories in marshes of St. Louis Bay, Mississippi

Salt marsh sediments generally undergo steady accumulation over time and thus are widely used to reconstruct the depositional histories of various anthropogenic contaminants derived from atmospheric and fluvial sources. Major hurricanes can significantly affect coastal landscapes by eroding and re-distributing sediment. Thus, each major hurricane can leave distinct signals in coastal wetland sediments. On the other hand, early-diagenetic remobilization of Fe and Mn in organic rich marsh sediment is a common phenomenon. However, remobilization of Fe and Mn across the redox boundary can induce remobilization of other trace elements and thus can disturb their depositional histories. Four short (~1m) sediment cores were collected from the fringing marshes of St. Louis Bay, Mississippi (located ~30km east of Hurricane Katrina's track) during 2010–2011 to investigate possible impacts of Hurricane Katrina (2005), and early-diagenetic remobilization of Fe and Mn, on trace metal and dioxin depositional histories in these sediments. Results from 210Pb, 137Cs, stable Cs, particulate organic carbon (POC), sediment bulk density and grain size indicate significant impact of hurricane event layers on anthropogenic stable Cs, while deposition profiles of V, Ni and Cr are impacted by Fe and Mn remobilization to a limited extent.

Distribution characteristics, sources, and ecological risk assessment of polycyclic aromatic hydrocarbons in sediments from the Qinhuangdao coastal wetland, China

Sixteen USEPA priority polycyclic aromatic hydrocarbons (PAHs) were analyzed by gas chromatography–mass spectrometry. Twenty-three samples were collected from the surface sediments of Qinhuangdao coastal wetlands in this survey. This research aimed to identify the PAHs contamination level, composition pattern, pollution sources, and assess the ecological risk of PAHs. The results showed that the sum of PAH concentrations ranged from 341.61ng/g to 4703.80ng/g (mean: 1367.80ng/g), which is higher than the reported values for different wetlands worldwide. Five- and four-ring PAHs (34.08% and 32.97% of Ʃ16PAHs, severally) were predominant in the wetland sediment. The PAH source distribution in the surface sediments was determined using diagnostic ratio and PCA/MLR. Consequently, multiple PAH sources were found. Of the total PAHs, 70.01% was derived from vehicular emission, 25.73% from coke oven, and 4.26% from petroleum-based product spills. The effect range low/effect range median (ERL/ERM) values indicated a low toxicity risk level. However, the DBahA concentrations exceeded the ERL level, and even the ERM level, in some stations. The mean effects range–median quotient (M-ERM-Q) suggests a low ecological risk for the PAHs, but a medium risk for some stations in the coastal wetland sediments.

On the application of contemporary bulk sediment organic carbon isotope and geochemical datasets for Holocene sea-level reconstruction in NW Europe

Bulk organic stable carbon isotope (δ13C) and element geochemistry (total organic carbon (TOC) and organic carbon to total nitrogen (C/N)) analysis is a developing technique in Holocene relative sea-level (RSL) research. The uptake of this technique in Northern Europe is limited compared to North America, where the common existence of coastal marshes with isotopically distinctive C3 and C4 vegetation associated with well-defined inundation tolerance permits the reconstruction of RSL in the sediment record. In Northern Europe, the reduced range in δ13C values between organic matter sources in C3 estuaries can make the identification of elevation-dependent environments in the Holocene sediment record challenging and this is compounded by the potential for post-depositional alteration in bulk δ13C values. The use of contemporary regional δ13C, C/N and TOC datasets representing the range of physiographic conditions commonly encountered in coastal wetland sediment sequences opens up the potential of using absolute values of sediment geochemistry to infer depositional environments and associated reference water levels. In this paper, the application of contemporary bulk organic δ13C, C/N and TOC to reconstruct Holocene RSL is further explored. An extended contemporary regional geochemical dataset of published δ13C, C/N and TOC observations (n=142) from tidal-dominated C3 wetland deposits (representing tidal flat, saltmarsh, reedswamp and fen carr environments) in temperate NW Europe is compiled, and procedures implemented to correct for the 13C Suess effect on contemporary δ13C are detailed. Partitioning around medoids analysis identifies two distinctive geochemical groups in the NW European dataset, with tidal flat/saltmarsh and reedswamp/fen carr environments exhibiting characteristically different sediment δ13C, C/N and TOC values. A logistic regression model is developed from the NW European dataset in order to objectively identify in the sediment record geochemical groups and, more importantly, group transitions, thus allowing the altitude of reference water levels to be determined. The application of this method in RSL research is demonstrated using the Holocene sediments of the Mersey Estuary (UK), in which δ13C, C/N and TOC variability is typical of that encountered in Holocene sediments from C3 coastal wetlands in NW Europe. Group membership was predicted with high probability in the depositional contexts studied and the accuracy of group prediction is verified by microfossil evidence. The method presented facilitates the application of δ13C, C/N and TOC analysis in RSL reconstruction studies in C3 vegetated wetlands throughout temperate NW Europe.

Modeled CO2 Emissions from Coastal Wetland Transitions to Other Land Uses: Tidal Marshes, Mangrove Forests, and Seagrass Beds

The sediments of coastal wetlands contain large stores of carbon which are vulnerable to oxidation once disturbed, resulting in high levels of CO2 emissions that may be avoided if coastal ecosystems are conserved or restored. We used a simple model to estimate CO2 emissions from mangrove forests, seagrass beds and tidal marshes based on known decomposition rates for organic matter in these ecosystems under either oxic or anoxic conditions combined with assumptions of the proportion of sediment carbon being deposited in either oxic or anoxic environments following a disturbance of the habitat. Our model found that over 40 years after disturbance the cumulative CO2 emitted from tidal marshes, mangrove forests and seagrass beds were approximately 70-80% of the initial carbon stocks in the top meter of the sediment. Comparison of our estimates of CO2 emissions with empirical studies suggests that 1) assuming 50% of organic material moves to an oxic environment after disturbance gives rise to estimates that are similar to CO2 emissions reported for tidal marshes; 2) field measurements of CO2 emissions in disturbed mangrove forests were generally higher than our modeled emissions that assumed 50% of organic matter was deposited in oxic conditions, suggesting higher proportions of organic matter may be exposed to oxic conditions after disturbance in mangrove ecosystems; and 3) the generally low observed rates of CO2 emissions from disturbed seagrasses compared to our estimates, assuming removal of 50% of the organic matter to oxic environments, suggests that lower proportions may be exposed to oxic conditions in seagrass ecosystems. There are significant gaps in our knowledge of the fate of wetland sediment carbon in the marine environment after disturbance. Greater knowledge of the distribution, form, decomposition and emission rates of wetland sediment carbon after disturbance would help to improve models.

New PCR primers targeting hydrazine synthase and cytochrome c biogenesis proteins in anammox bacteria.

PCR primers targeting genes encoding the two proteins of anammox bacteria, hydrazine synthase and cytochrome c biogenesis protein, were designed and tested in this study. Three different ecotypes of samples, namely ocean sediments, coastal wetland sediments, and wastewater treatment plant (WWTP) samples, were used to assess the primer efficiency and the community structures of anammox bacteria retrieved by 16S ribosomal RNA (rRNA) and the functional genes. Abundances of hzsB gene of anammox bacteria in South China Sea (SCS) samples were significantly correlated with 16S rRNA gene by qPCR method. And hzsB and hzsC gene primer pair hzsB364f-hzsB640r and hzsC745f-hzsC862r in combination with anammox bacterial 16S rRNA gene primers were recommended for quantifying anammox bacteria. Congruent with 16S rRNA gene-based community study, functional gene hzsB could also delineate the coastal-ocean distributing pattern, and seawater depth was positively associated with the diversity and abundance of anammox bacteria from shallow- to deep-sea. Both hzsC and ccsA genes could differentiate marine samples between deep and shallow groups of the Scalindua sp. clades. As for WWTP samples, non-Scalindua anammox bacteria reflected by hzsB, hzsC, ccsA, and ccsB gene-based libraries showed a similar distribution pattern with that by 16S rRNA gene. NH4+ and NH4+/Σ(NO3- + NO2-) positively correlated with anammox bacteria gene diversity, but organic matter contents correlated negatively with anammox bacteria gene diversity in SCS. Salinity was positively associated with diversity indices of hzsC and ccsB gene-harboring anammox bacteria communities and could potentially differentiate the distribution patterns between shallow- and deep-sea sediment samples. SCS surface sediments harbored considerably diverse community of Scalindua. A new Mai Po clade representing coastal estuary wetland anammox bacteria group based on 16S rRNA gene phylogeny is proposed. Existence of anammox bacteria within wider coverage of genera in Mai Po wetland indicates this unique niche is very complex, and species of anammox bacteria are niche-specific with different physiological properties towards substrates competing and chemical tolerance capability.

Effect of Fluctuating Salinity on Potential Denitrification in Coastal Wetland Soil and Sediments

The coastal wetlands of southern Louisiana provide an ideal environment for removing nitrate (NO₃⁻) from the Mississippi River before discharge into the Gulf of Mexico where it can contribute to hypoxia. However, denitrification, the primary mechanism of excess N removal, may be sensitive to the fluctuating salinities that characterize coastal wetlands. Salinity can shift from salt to fresh during river inflows, or from fresh to salt during storm surge events. This study investigated the impact of shifting salinity on potential denitrification rates in marsh soils and bayou sediments from the fresh and salt marshes of Brenton Sound estuary. Potential denitrification rates were quantified using bottle incubations for both short-term (2 d), and longer-term (11 d) studies. Fresh marsh sites had higher denitrification enzyme activity (DEA), but all soils and sediments achieved high denitrification when exposed to ideal conditions. Pulses of freshwater (0 ppt) in salt marsh soils decreased potential denitrification rates by 98% for an 11-d study, indicating the rapid opening of a freshwater diversion could significantly reduce that ability of coastal wetlands to provide significant nutrient-removal before discharge to the coastal ocean. Pulses of intermediate salinity (15 ppt) water stimulated denitrification rates in the fresh marsh soil by 75%, while full salinity seawater (35 ppt) suppressed potential denitrification by 73% for 11 d. This research demonstrates the sensitivity of the denitrifying microbial consortia to rapid shifts in salinity and this information can be used to guide water managers on maximizing NO₃⁻ removal in Mississippi River surface water diversions.

Rare earth element distribution in tropical coastal wetland sediments: a case study from Vembanad estuary, southwest India

Rare earth element (REE) geochemistry of sediments from Vembanad Coastal Wetland, southwest India, shows that light rare earth elements (LREEs) are the more abundant compared to middle rare earth element (MREE) and heavy rare earth element (HREE). We suggest that the textural parameters and the biochemical processes lead to the REE variation in this coastal wetland environment. Variations in the REE abundance are mainly controlled by the source materials deposited by riverine input. REE records in sediments showed spatial heterogeneity due to the influence of the physico-chemical conditions like, sedimentological conditions, salinity and depositional flux of the sediments along the wetland. Enriched values observed along the northern and southern stations are due to the increased riverine input. The REE ratios and enrichment factor values suggest that the abundance is in the order of LREE > MREE > HREE. Positive Eu anomaly is linked to the source sediments brought down from the catchment areas of the Periyar River and Muvattupuzha River. However, Ce anomaly suggests no significant variation in the oxidation condition at the sites of deposition.