Coast Of Australia Research Articles

Nitrogen-cycling genes in oyster reefs and surrounding sediments: Relationships with environmental factors and respective nitrogen rates

To investigate nitrogen (N) cycling in oyster reef habitats along the East coast of Australia, we assessed N-cycling gene abundances in oyster shell biofilms and surrounding sediments, and explored their correlation with environmental factors and respective N rates. We found higher abundances of the denitrification gene nosZII in oyster shell biofilms, while there were not significant differences in the denitrification genes nirS and nirK between oyster biofilms and sediments. Additionally, oyster shell biofilms had a lower (nirS + nirK)/nosZII ratio, indicating a greater capacity for N removal and limited nitrous oxide release compared to sediments. Abundance of nirS, nirK, and dissimilatory nitrate reduction to ammonium (nrfA) genes in sediments decreased with increasing content of organic material, suggesting the influence of large-scale environmental conditions. N-cycling gene abundances did not relate to N rates, emphasising the importance of investigating microbial genes to enhance our understanding of the N cycle in oyster reef habitats.

An Analysis of the Dawn Vocalisation of a Male Grey Shrikethrush (Colluricincla harmonica)

ABSTRACT The vocalisations of the Grey Shrikethrush are loud, clear, pleasing to the ear, and a stand-out feature of the bird song in areas in which they occur. However, data on these vocalisations in the literature are sparse, as are any attempts at either quantitative or qualitative analysis. Consequently, almost nothing is known about the character of this very obvious song. We made eight recordings of the dawn song of a male individual at the start of the breeding season on the south-east coast of Australia, in spotted gum forest, 350 km south of Sydney. The song comprised discreet groups of notes which we call syllables. The syllables are sung about every 5-10 seconds, and the individual produced 20 different syllables over the course of the eight recordings. Various analyses revealed that new syllables were added gradually over the recording period, and reached a maximum number at the sixth recording, nine days after the first recording. The frequency of use of particular syllables varied markedly, it was common for some syllables to be used as repeats, particular heterogeneous syllable couplets were commonly used as alternations, and the sequence of syllables could be remarkably similar between different recordings. We suggest a way forward to determine whether these patterns are a common feature of the vocalisations of this species.

The Plane of Cicadas: On the Possibility of Making Kin through Musicking

This autoethnographic exposition details a short series of musicking encounters aimed at making kin in a rainforest-covered mountain on unceded Djiru Country on the east coast of Australia. Each encounter consisted of a short hike and a discrete musical encounter with local subjectivities. The inquiry considers the place of the walking in the musicking, and my joint response-ability with my saxophone (or the ways in which we apprehend and respond to other subjectivities) in each encounter. I argue that the hiking and discrete sonic encounters became entangled processes, in which my epistemological capacity and music-making practice were challenged and the possibility of kinship resides.

La Niña Pushes an Endangered Temperate Soft Coral Species to the Brink of Localised Extinction

Extreme weather events such as floods are becoming more frequent, and pose a substantial threat to Australia’s nearshore marine communities. In March 2021, a 450 km stretch of the eastern NSW coastline experienced a La Niña-related rainfall event over several days, resulting in large volumes of freshwater ingress to marine systems. Port Stephens, an estuary 250 km north of Sydney, recorded its highest week of rainfall on record. This was followed by two more flood events in March 2022 and June 2022. Prior to 2021, the marine-dominant Eastern Port of Port Stephens was home to the world’s largest aggregations of Dendronephthya australis, an endangered species of soft coral endemic to the south-east coast of Australia. Using data from a 2019–2022 D. australis monitoring program in Port Stephens, spanning the unprecedented series of flood events in 2021–2022, we detail the impacts of flood events on the population. Prior to the floods, aggregations of colonies were persisting, and individuals were growing at two of the four monitored sites. However, flooding in March 2021 caused a 91% decline in the remaining areal extent of D. australis. Modelling of likely causative factors highlighted water depth as the most significant environmental variable correlated with coral loss. Corals in shallower waters experienced lower salinity and were the most impacted. Continued monitoring during 2021 and 2022 documented the loss of all remaining aggregations in the estuary after further La Niña-related rainfall events, to the point where D. australis is now on the brink of localised extinction.

Constraints on the expanse of Greater India in the Early Cretaceous from radiolarians

Defining the original size of Greater India is a critical parameter for reconstructing the kinematics and timing of the India–Asia collision. Early Cretaceous paleontology-based paleoceanographic and paleogeographic reconstruction for the northern coast of East Gondwanaland provides independent constraint for the extent of Greater India. In this study, newly discovered Early Cretaceous radiolarian faunas from the Tethys Himalaya were objectively compared with non-Tethyan austral faunas from the Argo Abyssal Plain (AAP) and the typical Tethyan fauna from Italy using the Hayashi's Quantification Method Type III. We found that (1) The occurrence of austral cold-water radiolarian species at the western coast of Australia and northern coast of Greater India is attributable to northward intrusion of circumantarctic cold water via a seaway newly opened during the breakup of East Gondwanaland from ∼136 Ma; (2) Gyangze was under shallow warm-water conditions with typical Tethyan radiolarian fauna (Cluster 3) until the late Valanginian when cold-water species (Cluster 1) arrived from the south. A water mass boundary shift from Tethyan species dominant to austral cold-water species dominant in Gyangze indicates its lower paleolatitude than Ocean Drilling Program (ODP) Leg 123, Site 765 in the AAP; (3) The same austral cold-water radiolarian-bearing intervals from the late Berriasian to Valanginian indicate that Kangma in southern Tibet was proximal to ODP Site 765; (4) Different radiolarian faunas in Italy–Zhongba, Kangma, and the AAP reveal complex water conditions under different degrees of effect by Tethyan warm water and austral cold water at different water depths during the Barremian to Aptian. This radiolarian biogeography is highly consistent with the paleomagnetic reconstruction and indicates that eastern Greater India extended to the Exmouth Plateau (>2400 km) during the Early Cretaceous.

Multiweek tropical cyclone prediction for the Southern Hemisphere in ACCESS‐S2: Maintaining operational skill and continuity of service

AbstractThe skill of subseasonal (multiweek) forecasts of tropical cyclone (TC) occurrence over the Southern Hemisphere is examined in the Australian Bureau of Meteorology's (BoM) multiweek to seasonal prediction system, ACCESS‐S2. Relative to its predecessor, ACCESS‐S1, ACCESS‐S2 shows improved biases in spatial TC frequency in the South Pacific and Southwest Indian Ocean. However, there is no improvement to the known negative bias in TC frequency off the coast of NW Australia. The ability of ACCESS‐S2 to provide probabilistic forecasts of TC occurrence for the Southern Hemisphere on multiweek timescales is examined using reliability measures and Brier skill scores. For the period November–February 1990–2012, both ACCESS‐S1 and ACCESS‐S2 show positive skill relative to climatology for calibrated forecasts out to week 5. However, the skill of ACCESS‐S2 is slightly reduced compared to ACCESS‐S1 at all lead times, which may be due to the fewer number of ensemble members available. For the full ACCESS‐S2 hindcast period, November–April 1981–2018, ACCESS‐S2 again shows positive skill of calibrated forecasts over climatology out to week 5. For weeks 1–2, skill is reduced compared to the shorter 1990–2012 period; whereas it is marginally improved for longer lead times (weeks 3–5). Use of lagged ensembles, an alternative linear regression calibration, as well as removing weaker model TCs were examined to potentially improve the skill of ACCESS‐S2 forecasts; however, none of these methods were able to significantly increase skill at all lead times. Continued use of the original calibration method is therefore recommended in order to retain skill and continuity of service of the BoM operational and public multiweek TC forecasts.

Propagation of ship noise in shallow water over a high shear-speed seabed

Assessing the acoustic impact of shipping close to the coast and within ports and harbours requires an understanding of the propagation of underwater sound in these environments. In the Australian context, this often means propagation over seabeds with shear speeds approaching the in-water sound speed, a situation that results in very different propagation conditions to the low shear speed sediments more commonly encountered in other parts of the world. Acoustic propagation modelling approaches were explored using a dataset of continuous underwater recordings featuring a mix of shipping, industrial, and recreational activities, acquired from 23 Jul to 16 Sep 2022 in Cockburn Sound, off the west coast of Australia. Cockburn Sound is a sheltered marine embayment with a relatively uniform water depth of 17–20 m in the 5 km × 11 km central portion of the basin, with a thin, variable thickness (0.5 m–5 m) layer of silty sediments overlying a calcarenite basement. The results of modelling the calcarenite basement as an equivalent fluid were compared to those obtained by modelling it as an elastic solid, and with measurements. Subsequent implications of the choice of geoacoustic model will be discussed

Humpback whales increase the length of their songs during nearby airgun operations

There is concern that noise from “airguns” used during oil and gas exploration may cause behavioral changes in marine mammals. One important behavior of some mysticete whales is the production of songs which are likely used as reproductive displays. Humpback whales have conspicuous songs but there are few studies on the impact of noise on their singing behavior. Here, we test the hypothesis that airgun noise causes individual humpback whales to reduce the duration of their songs. In a series of experiments off the east coast of Australia, we exposed migrating humpback whales to airguns. We tracked and recorded singing males when we towed airguns through the study area as well as when there were no vessels or airguns present. We also noted interactions between singers and nearby conspecifics. Humpbacks usually stop singing when they join with others, and so joining is both a predictor of song duration and a biologically relevant outcome. Contrary to expectations, whales that were already singing prior to airgun exposure produced significantly longer songs than unexposed singers. However, the small number of whales that started singing during airgun exposure produced significantly shorter songs.

Does female choice for song complexity drive sexual selection in humpback whales?

Male humpback whales (Megaptera novaeangliae) sing a long, stereotyped, and culturally transmitted song display. While song likely functions in sexual selection, whether it is primarily directed at females and/or males is still debated. Most males within a single population sing the same, slowly evolving song type. However, multiple humpback whale song revolutions (where a song introduced from a neighboring population rapidly and completely replaces the existing song) have spread across the South Pacific region from the east coast of Australia to French Polynesia. How cultural evolution and sexual selection interact in this complex vocal display is not well understood. We investigated song-mediated female mate choice in humpback whales using 20 years of paternity data and song recordings from individually identified males in the small, South Pacific population of New Caledonia. As mating has never been observed in humpback whales, we assessed male reproductive success (calf paternity) and song complexity (unit repertoire and song length) of individual males. We found that males who successfully sired offspring had on average higher song complexity than males who did not sire offspring. This suggests females may select mates at least partially based on song complexity, and thus, song being primarily directed at females.

The weather determines the number of cases of tick paralysis in dogs and cats in eastern Australia, caused by Ixodes holocyclus, the eastern paralysis tick.

We studied over 222,000 cases of emergency veterinary consultations in four regions along the eastern coast of Australia. We found that cases of tick paralysis (TP) caused by the eastern paralysis tick, Ixodes holocyclus, accounted for 7.5% of these cases: >16,000 cases. The season of TP and the number (prevalence) of TP cases varied among regions and over the years. Our study of the association between weather and (i) the start of the season of TP, and (ii) the number of TP cases revealed much about the intricate relationship between the weather and I. holocyclus. We studied the effect of the hypothetical availability of isoxazoline-containing tick-preventative medicines and found that an increase in the availability of these medicines had significantly contributed to the decrease in TP cases. We found that the weather in winter accounted for the time of the year the season of TP starts whereas the weather in summer accounted for the number of TP cases in the TP season. Last, through a study of the effects of shifts in the climate under four hypothetical scenarios (warmer/cooler and drier/wetter than average), we propose that the start of the season of TP depends on how soon the weather in winter becomes suitable for the activity (e.g. host-seeking) and the development of I. holocyclus nymphs, and that the number of TP cases during the TP season depends on how many engorged female ticks and their eggs survive during summer.

High‐latitude marginal reefs support fewer but bigger corals than their tropical counterparts

Anthropogenic impacts are typically detrimental to tropical coral reefs, but the effect of increasing environmental stress and variability on the size structure of coral communities remains poorly understood. This limits our ability to effectively conserve coral reef ecosystems because size specific dynamics are rarely incorporated. Our aim is to quantify variation in the size structure of coral populations across 20 sites along a tropical‐to‐subtropical environmental gradient on the east coast of Australia (~ 23 to 30°S), to determine how size structure changes with a gradient of sea surface temperature, turbidity, productivity and light levels. We use two approaches: 1) linear regression with summary statistics (such as median size) as response variables, a method frequently favoured by ecologists and 2) compositional functional regression, a novel method using entire size–frequency distributions as response variables. We then predict coral population size structure with increasing environmental stress and variability. Together, we find fewer but larger coral colonies in marginal reefs, where conditions are typically more variable and stressful, than in tropical reefs. Our model predicts that coral populations may become gradually dominated by larger colonies (> 148 cm2) with increasing environmental stress. Fewer but bigger corals suggest low survival of smaller corals, slow growth, and/or poor recruitment. This finding is concerning for the future of coral reefs, as it implies that current marginal populations, or future reefs in increasingly stressful environmental conditions may have low recovery potential. We highlight the importance of continuously monitoring changes to population structure over biogeographic scales.

What’s at Play: Humpback Whale Interaction with Seaweed Is a Global Phenomenon

The use of objects by cetaceans is well known, and their ability to interact with their environment in complex behaviours has been demonstrated previously. However, baleen whales, including humpback whales (Megaptera novaeangliae), are less often observed to perform object use, but this behaviour might be more common than previously thought. Only a few isolated observations of interactions with seaweed have been reported in the scientific literature to date. The recovery of humpback whale populations, as well as the rise of technology such as unmanned aerial vehicles (UAVs) and the use of social media, allow for a new assessment of this object interaction. Here, we describe in detail three instances of “kelping” on the east coast of Australia derived from aerial observations. A summary of over 100 separate and unrelated events drawn from social media, documented by photographs and videos, suggests that this form of interaction with seaweed is observed across different populations. The form of interaction with seaweed is similar between regions, predominantly displayed between the rostrum and dorsal fin. This behaviour may be playful but could also serve additional benefits in the context of learning and socializing, as well as ectoparasite removal and skin treatment by utilizing brown algae’s antibacterial properties. Establishing this type of behaviour as distributed across different populations is important to better understand the species’ habitat preferences.

Genetic divergence of farmed blue mussels (Mytilus sp.) in Australian waters

As valuable ecosystem engineers, blue mussels, are one of the most ecologically and economically important bivalve species in Australia. The need for understanding levels of genetic diversity in both wild and farmed populations of Australian mussels is ever-growing due to the increased risk of disease and decreased fitness associated with the constantly changing global environment. This study, therefore, investigated the phylogenetic relationship, and the genetic variation of farmed blue mussels (Mytilus sp., previously noted as M. galloprovincialis, M. planulatus or a hybrid of both) at four sites along the south coast of Australia, Victoria, South Australia, Western Australia, and Tasmania. Analyses of two genes, Cytochrome c oxidase subunit 1 gene (mtCOI) and the nuclear gene Elongation factor 1 alpha (EF1a) was completed for all mussels sampled. The phylogenetic reconstruction of mtDNA sequences was not able to cluster the samples into species. The analyses of the nuclear marker (EF1a) returned similar results, except that we could conclude the Australian samples not being M. chilensis. Furthermore, the analyses of EF1a revealed the presence of hybridisation, a pattern commonly observed in mussels. The genetic diversity analysis of mtCOI demonstrated that samples from Victoria and Western Australia had the highest genetic diversity, while South Australia had the lowest. When analysing the nuclear EF1a sequences, mussels from Western Australian had the lowest genetic diversity, and the Victorian samples had the highest.The study extended our knowledge on the population genetic variation of farmed blue mussels (Mytilus sp.) along the south coast of Australia. The identified genetic differences between the four southern states of Australia could be interesting for the aquaculture industry when aiming for more adaptable and profitable stocks.

Utility of body and otolith morphometry to discriminate cryptic juveniles of two sympatric red snappers (Perciformes: Lutjanidae).

The sympatric red snappers, Lutjanus erythropterus and Lutjanus malabaricus, are highly valued by commercial and recreational fishers along the tropical northern coasts of Australia and throughout their distribution. Studies on the life history and ecology of these congeners are confounded by difficulties in distinguishing the cryptic juveniles of each species (i.e., < 200 mm total length). This study aimed to validate a robust and cost-effective method to discriminate these juveniles using body and/or otolith morphometric data in a multivariate analysis. Juvenile samples were collected from the northwest (n = 71) and northeast (n = 19) coasts of Australia, and species identification was confirmed using DNA barcoding. The most parsimonious multivariate models achieved accurate species prediction rates of 98.8%, which consisted of just three body variables (dorsal fin length, the distance from the snout to the anterior edge of the eye, and either jaw length or distance from the snout to the preoperculum). The high level of discrimination for these cryptic juveniles highlights the robustness of this morphometric approach. The slightly lower rate of discrimination using otolith morphology (84.9%) was associated with greater regional variation in L. malabaricus between the northwest and northeast coasts. Slight variations in otolith shape are typically used to determine stock structure, which highlights the potential need to collect samples over a broader area of a species geographic range when using an otolith morphometric discrimination model. The method outlined in this study could be applied to distinguish other cryptic congeneric fish species, including from archived otolith collections. Moreover, this method has the potential to be utilized in assessing species compositions using body measurements from in situ stereo-video.

“Weather, People, Ship”: The Environment’s Impact on Cook’s First Voyage into the Pacific

This research explores how the eighteenth-century Pacific environment shaped James Cook’s first voyage into the South Seas. Although a multitude of different investigations and numerous biographies on Cook examine this exploration, there is limited analysis of its environmental dimension. This study offers a new perspective on this famous voyage – in terms of cultural and technological impediments – from the Island of Tahiti to Aotearoa New Zealand, and to the Eastern Coast of Australia. Through a reading of expedition accounts that focus on environmental elements, it is possible to shed new light on the failure to discover the Southern Continent, uncover mistakes in observations about new territories, and understand conflicts with Indigenous people. The aim of this article is to widen the discussion about eighteenth-century European explorers by highlighting the impact of rain, winds, heat, and waters on human will and actions.

Implications of Working from Home for the Design of Healthy Work Environments in the Post-Pandemic City

Remote work in cities is growing in popularity, fuelled by ongoing technological advances, the globalized knowledge economy, changing lifestyle preferences, the need to empower individuals, and – more recently – the eff ects of COVID-19. Social distancing measures introduced during the pandemic have inadvertently shown that a substantial proportion of work can be done from home or from third spaces such as co-working spaces. This paper off ers a critical appraisal of the implications of this trend for neighbourhood planning and workplace design. The appraisal is in three parts. First, to set the scene, we review recent scholarship on changing work practices in the post-pandemic city. Second, we offer a summative account based on empirical data from a survey conducted by the City of Gold Coast in Australia. This survey explored the spatial distribution of remote, nomadic, and home-based workers in cities in order to discover certain socio-economic, design and built environment features that relate to this distribution. This illustrates the impact that an uptake of home-based work has for urban planning and community design. Third, we look at some of the working from home implications for career progression and productivity, as well as physical and mental health. Based on perspectives from architectural science, environmental psychology and design, this part of the paper employs human-building interaction design scholarship to argue for the design of healthy work environments – both at home and in neighbourhoods – that increase productivity, reduce sick days, and yield be er health outcomes for the home-based workforce.

The influence of oyster reefs and surrounding sediments on nitrogen removal – An in-situ study along the East coast of Australia

Oyster reefs play a crucial role in the removal of nitrogen (N) from aquatic systems by facilitating nutrient regeneration and denitrification, both in their tissues and shells and surrounding sediments. However, we still have a limited understanding about the contribution of each component of the reefs (e.g. oysters vs sediments) to N processes, and whether rates are dependent on site-specific characteristics. To address these knowledge gaps, we conducted an experiment across six oyster reefs along 1080 km of the Eastern Australian coast with different sediment characteristics. By using in-situ clear and dark incubation chambers, we assessed how benthic metabolism, nutrient and dinitrogen gas (N2) fluxes varied among the following treatments: ‘oysters’, ‘sediments’, and ‘sediments + oysters’ that were used to represent components of the whole reef habitat (i.e. reef matrix vs surrounding sediments vs the interaction among them, respectively), and sites. We found that during dark conditions and at siltier sites, N2 effluxes from oysters can be up to 23 times higher than sediments, while N2 effluxes from chambers with both sediments and oysters were similar to sediment treatments, and lower than oyster treatments. These results can be explained by sediment processes including nutrient assimilation by benthic microalgae and/or lower nutrient diffusion into interstitial space. Additionally, oyster treatments showed an uptake of nitrate (NO3-) that was likely converted into N2, whereas sediment treatments showed an overall release of NO3-. In dark conditions, ammonium (NH4+) fluxes remained consistent across treatments and sites, indicating that any exports from oyster excretion (in those treatments including oysters) were either counterbalanced by or comparable to exports from sediments. This study provides evidence that the crucial contribution of oyster reefs to N removal is dependent on interactions between reef components and environmental factors.

Instrumentation and Data Interpretation in Transportation Geotechnics

AbstractTransportation networks on the eastern coast of Australia are often built on or traverse coastal flood plains and marine clays with unfavourable soil conditions. In the past two decades, a significant number of laboratory investigations were carried out in soft soil improvement using Prefabricated Vertical Drains (PVDs) combined with vacuum-assisted surcharge preloading. In addition, significant research efforts were made to reduce the maintenance costs of railway tracks and increase their longevity by using synthetic inclusions such as geocomposites, geogrids and shock mats. These research outcomes were implemented and validated in practice through several field investigations along the eastern coast of Australia. This paper demonstrates the significance of instrumentation and data interpretation in geotechnical field investigations through 6 case histories. Field trials including Port of Brisbane Land Reclamation, Ballina Bypass Upgrade and Sandgate Rail Separation Projects were presented highlighting innovative ways of monitoring the performance of PVDs with vacuum and non-vacuum surcharge preloading. Also, railway track design improvements using geosynthetic and shock mats were discussed through Bulli and Singleton trial track case studies. Further, the heavy haul track testing facility at Russell Vale, New South Wales, was discussed as an alternative for expensive and time-consuming field trials.

Using intervention analysis to evaluate the trends in release rates of recreational fisheries following extensive management changes

Changes to management of a fisheries resource are often required to ensure ongoing sustainability. However, such changes can sometimes lead to unintended effects such as increased release rates and associated post-release mortality. These effects may be highly variable between species and areas. Recreational fishing management changes were introduced on the west coast of Australia in 2009/10 to recover stocks of demersal scalefish. Key changes included reducing mixed species bag limits across management zones and increasing the minimum size limit for one species in some management zones. The restrictive catch limits resulted in increased release rates of key demersal species. However, whether such increases are significant and sustained over time, and thus of management concern, have not been evaluated. We carried out intervention time series analysis to evaluate the impact of management changes on release rates of four key demersal species for the recreational sector in metropolitan and regional management zones covering ∼8° latitude using an 18-year time series of charter recreational fishery data from July 2002 to January 2020. We observed varying responses in release rates by species and zones, the most common of which were a step increase, a ramp and a temporary increase that decayed. These responses may be related to targeted management changes which influenced fisher behaviour, perceived recreational value of some species and recruitment variation. Our study demonstrates that intervention analysis, which has seen limited use in this context, can assist in evaluating the impact of management changes on different species for recreational fisheries.

Identification and quantification of Acanthamoeba spp. within seawater at four coastal lagoons on the east coast of Australia

Acanthamoeba is an opportunistic free-living heterotrophic protist that is the most predominant amoeba in diverse ecological habitats. Acanthamoeba causes amoebic keratitis (AK), a painful and potentially blinding corneal infection. Major risk factors for AK have been linked to non-optimal contact lens hygiene practices and Acanthamoeba contamination of domestic and recreational water. This study investigated the incidence and seasonal variation of Acanthamoeba spp. within coastal lagoons located on the eastern coast of Australia and then examined the association between Acanthamoeba and water abiotic factors and bacterial species within the water.Water samples were collected from four intermittently closed and open lagoons (ICOLLs) (Wamberal, Terrigal, Avoca and Cockrone) every month between August 2019 to July 2020 except March and April. qPCR was used to target the Acanthamoeba 18S rRNA gene, validated by Sanger sequencing. Water abiotic factors were measured in situ using a multiprobe metre and 16S rRNA sequencing (V3-V4) was performed to characterise bacterial community composition. Network analysis was used to gauge putative associations between Acanthamoeba incidence and bacterial amplicon sequence variants (ASVs).Among 206 water samples analysed, 79 (38.3%) were Acanthamoeba positive and Acanthamoeba level was significantly higher in summer compared with winter, spring, or autumn (p = 0.008). More than 50% (23/45) water samples of Terrigal were positive for Acanthamoeba which is a highly urbanised area with extensive recreational activities while about 32% (16/49) samples were positive from Cockrone that is the least impacted lagoon by urban development. All sequenced strains belonged to the pathogenic genotype T4 clade except two which were of genotype clades T2 and T5. Water turbidity, temperature, intl1 gene concentration, and dissolved O2 were significantly associated with Acanthamoeba incidence (p < 0.05). The ASVs level of cyanobacteria, Pseudomonas spp., Candidatus spp., and marine bacteria of the Actinobacteria phylum and Acanthamoeba 18S rRNA genes were positively correlated (Pearson's r ≥ 0.14). The presence of Acanthamoeba spp. in all lagoons, except Wamberal, was associated with significant differences in the composition of bacterial communities (beta diversity).The results of this study suggest that coastal lagoons, particularly those in urbanised regions with extensive water recreational activities, may pose an elevated risk to human health due to the relatively high incidence of pathogenic Acanthamoeba in the summer. These findings underscore the importance of educating the public about the rare yet devastating impact of AK on vision and quality of life, highlighting the need for collaborative efforts between public health officials and educators to promote awareness and preventive measures, especially focusing lagoons residents and travellers.