Coarse Cereal Research Articles

Effects of cereal flour types and sourdough on dough physicochemical properties and steamed bread quality.

Coarse cereals have been promoted for their health benefits, and sourdough is used to improve their steamed bread sensory acceptance. However, grains vary in dough physiochemical properties and steamed bread-making performance. This study investigated the effects of yeast and sourdough fermentation on the biochemical, textural, and flavor properties of dough and steamed bread of eight grain types. Results indicated that sourdough dough had a lower pH and higher total titrable acidity compared with yeast group. The texture of sourdough-steamed bread was significantly improved with reduced hardness and enhanced springiness. Microstructure revealed that sourdough resulted in starch surface corrosion and less amylopectin recrystallization. Aldehydes, alcohols, and esters are more dominant in sourdough group than yeast group. Foxtail millet and sorghum steamed breads exhibited the highest performances in texture, flavor, and sensory evaluation. This could provide a theoretical basis for producing coarse cereal products with desirable quality.

Enhancing rutin accumulation in Tartary buckwheat through a novel flavonoid transporter protein FtABCC2

Tartary buckwheat (Fagopyrum tataricum) is an annual coarse cereal from the Polygonaceae family, known for its high content of flavonoid compounds, particularly rutin. But so far, the mechanisms of the flavonoid transport and storage in Tartary buckwheat (TB) remain largely unexplored. This study focuses on ATP-binding cassette transporters subfamily C (ABCC) members, which are crucial for the biosynthesis and transport of flavonoids in plants. The evolutionary and expression pattern analyses of the ABCC genes in TB identified an ABCC protein gene, FtABCC2, that is highly correlated with rutin synthesis. Subcellular localization analysis revealed that FtABCC2 protein is specifically localized to the vacuole membrane. Heterologous expression of FtABCC2 in Saccharomyces cerevisiae confirmed that its transport ability of flavonoid glycosides such as rutin and isoquercetin, but not the aglycones such as quercetin and dihydroquercetin. Overexpression of FtABCC2 in TB hairy root lines resulted in a significant increase in total flavonoid and rutin content (P < 0.01). Analysis of the FtABCC2 promoter revealed potential cis-acting elements responsive to hormones, cold stress, mechanical injury and light stress. Overall, this study demonstrates that FtABCC2 can efficiently facilitate the transport of rutin into vacuoles, thereby enhancing flavonoids accumulation. These findings suggest that FtABCC2 is a promising candidate for molecular-assisted breeding aimed at developing high-flavonoid TB varieties.

Endophytic fungi in buckwheat seeds: exploring links with flavonoid accumulation.

Buckwheat is a famous edible and medicinal coarse cereal which contain abundant of bioactive flavonoids, such as rutin. In this study, the composition and diversity of endophytic fungi in eight different buckwheat seeds were analyzed by high-throughput sequencing of ITS rDNA. Results showed that, the fungal sequences reads were allocated to 272 OTUs, of them, 49 OTUs were shared in eight buckwheat seeds. These endophytic fungi could be classified into 6 phyla, 19 classes, 41 orders, 79 families, 119 genera, and 191 species. At genus level, Alternaria sp. was the domain fungal endophyte. Besides, fungal endophytes belonged to the genera of Epicocum, Cladosporium, Botrytis, Filbobasidium, Stemphylium, and Vishniacozyma were highly abundant in buckwheat seeds. The total flavonoids and rutin contents in tartary buckwheat cultivars (CQ, XQ, CH, K2) were much higher than those in common buckwheat cultivars (HT, T2, T4, T8). For tartary buckwheat cultivars, the total flavonoids and rutin contents were ranging from 2.6% to 3.3% and 0.9% to 1.3%, respectively. Accordingly, the tartary buckwheat samples displayed stronger antioxidant activity than the common buckwheat. Spearman correlation heat map analysis was successfully found that certain fungal species from the genera of Alternaria, Botryosphaeria, Colletorichum and Diymella exhibited significant positive correlation with flavonoids contents. Results of this study preliminary revealed the fungi-plant interaction relationship at secondary metabolite level, and could provide novel strategy for increasing the flavonoids accumulation of buckwheat seeds, as well as improving their quality.

New strategies to address world food security and elimination of malnutrition: future role of coarse cereals in human health.

As we face increasing challenges of world food security and malnutrition, coarse cereals are coming into favor as an important supplement to human staple foods due to their high nutritional value. In addition, their functional components, such as flavonoids and polyphenols, make them an important food source for healthy diets. However, we lack a systematic understanding of the importance of coarse cereals for world food security and nutritional goals. This review summarizes the worldwide cultivation and distribution of coarse cereals, indicating that the global area for coarse cereal cultivation is steadily increasing. This paper also focuses on the special adaptive mechanisms of coarse cereals to drought and discusses the strategies to improve coarse cereal crop yields from the perspective of agricultural production systems. The future possibilities, challenges, and opportunities for coarse cereal production are summarized in the face of food security challenges, and new ideas for world coarse cereal production are suggested.

An economic analysis of crop diversification and dynamics of cropping pattern in Karnataka, India

The extent and nature of crop diversification and dynamics of cropping pattern in Karnataka state has been analyzed using secondary data collected from different publish sources of Directorate of Economics and Statistics (DES), Bangalore, Karnataka from a period of 1998–99 to 2020–21 (i.e., 23 years). Composite Entropy Index (CEI), double log model and Markov chain analysis were applied to assess the determinants of crop diversification and dynamics of cropping pattern in Karnataka. Results of C.E.I indicates that Kodagu, Dakshina kannada, Yadgir, Udupi and Shivamogga has low crop diversification. Although Karnataka’s crop diversification values show a positive trend but its coefficient value is almost zero. It shows that there is a threat for Karnataka’s crop diversification. The transition probability matrix diagram indicated that although cereals and millet groups occupied a larger share in terms of area under crops, it has a negative net gain in the probability of area retention. Double log model results revealed that the variable like MSP of coarse cereal, percentage of gross irrigated area to net irrigated area, credit inflow in to agricultural sector by the scheduled commercial bank and length of national highways are influencing the crop diversification positively. Therefore, the establishment of fundamental infrastructure, such as reliable irrigation water supply, access to credit, and well-developed transportation networks, is a crucial prerequisite for creating favorable conditions to promote agricultural development and crop diversification. These parameters have been identified as influential factors that shape the nature and extent of crop diversification. By addressing these infrastructure needs, it becomes possible to foster an environment conducive to agricultural growth and facilitate the process of diversification.

Precooking Technology and Product Development of Coarse Cereals

AbstractThe purpose of this study is to develop instant coarse cereal porridge to improve the convenience of coarse cereals by precooking. The effects of cooking time on the rehydration rate and gelatinization degree of coix seeds, red beans, lotus seeds, wheat kernels, and glutinous rice are investigated using the atmospheric pressure precooking process. Cooking time and quality variations of the coarse cereals are analyzed before and after precooking. The optimal cooking times for coix seeds, red beans, lotus seeds, wheat kernels, and glutinous rice under atmospheric pressure cooking are 110, 65, 40, 40, and 35 minutes (abbr. min), respectively. The rehydration rates are 171.5%, 190%, 170.67%, 224%, and 182.67%, respectively, and the gelatinization degrees are 90%, 74.54%, 80.1%, 92.85%, and 96.97%, respectively. The cooking times of pre‐cooked and pressed coix seeds, red beans, lotus seeds, wheat kernels, and glutinous rice are 10, 8, 12, 10, and 6 min, respectively. Compared to coarse cereals without precooking, the cooking time is reduced by 6.6, 6.5, 3, 4, and 2.3 times, respectively. Instant porridge prepared by combining precooked coarse cereals and dried fruits and vegetables can be consumed after soaking in boiling water for 20 min or boiling for 10 min.

Is India Moving Away from Coarse Cereals? Examining Evidence from Trends in Rice, Wheat and Coarse Cereals

India’s agricultural production of cereals since the 1950s mainly comprises of rice, wheat and coarse cereals. Data shows that coarse cereals despite having maximum area under cultivation post independence and a high growth rate of production, have registered a declining trend in the recent decades. With global warming becoming a threat to monsoon- dependent grains like rice and wheat, India needs to start focusing more on coarse cereals. They are not only climate resilient using one-third the amount of water as compared to rice and wheat, but are also nutritionally superior to these grains. Data analysis for top five rice, wheat and coarse cereal producing states show that the decadal growth rates and yield per hectare have been declining for rice and wheat. Detailed inter-state comparisons are done to evaluate various aspects of these foodgrains. Finally, a fixed effects regression model is estimated to analyze the effect of area, annual rainfall, fertilizer consumption and MSP on the productivity of coarse and fine cereals, results show coarse cereals to be less dependent on rainfall and fertilizer use.

A Novel R2R3-MYB Transcription Factor FtMYB22 Negatively Regulates Salt and Drought Stress through ABA-Dependent Pathway.

Tartary buckwheat (Fagopyrum tataricum Gaertn.) is a coarse cereal with strongly abiotic resistance. The MYB family plays a regulatory role in plant growth, development, and responses to biotic and abiotic stresses. However, the characteristics and regulatory mechanisms of MYB transcription factors in Tartary buckwheat remain unclarified. Here, this study cloned the FtMYB22 gene from Tartary buckwheat, and investigated its involvement in responding to individual water deficit and salt stress in Arabidopsis. Sequence analysis highlighted that the N-termini of FtMYB22 contained two highly conserved SANT domains and one conserved domain from the SG20 subfamily. Nucleus-localized FtMYB22 did not have individual transcriptional activation activity. Water deficiency and salt stress induced the high expression of the GUS gene, which was driven by the promoter of FtMYB22. Yeast stress experiments showed that the overexpression of FtMYB22 significantly reduced the growth activity of transgenic yeast under water deficit or salt stress. Consistently, the overexpression of FtMYB22 reduced the salt and water deficit stress resistance of the transgenic plants. In addition, physiological parameters showed that transgenic plants had lower proline and antioxidant enzyme activity under stress conditions. Compared to the wild-type (WT), transgenic plants accumulated more malondialdehyde (MDA), H2O2, and O2−; they also showed higher ion permeability and water loss rates of detached leaves under stress treatments. Notably, FtMYB22 was involved in plant stress resistance through an ABA-dependent pathway. Under stress conditions, the expression of RD29A, RD29B, PP2CA, KIN1, COR15A, and other genes in response to plant stress in transgenic lines was significantly lower than that in the WT (p < 0.05). Furthermore, yeast two-hybrid assay showed that there was a significant interaction between FtMYB22 and the ABA receptor protein RCAR1/2, which functioned in the ABA signal pathway. Altogether, FtMYB22, as a negative regulator, inhibited a variety of physiological and biochemical reactions, affected gene expression and stomatal closure in transgenic plants through the ABA-dependent pathway, and reduced the tolerance of transgenic Arabidopsis to water deficiency and salt stress. Based on these fundamental verifications, further studies would shed light on the hormone signal response mechanism of FtMYB22.

Metabolomics Reveals Nutritional Diversity among Six Coarse Cereals and Antioxidant Activity Analysis of Grain Sorghum and Sweet Sorghum.

Coarse cereals are rich in dietary fiber, B vitamins, minerals, secondary metabolites, and other bioactive components, which exert numerous health benefits. To better understand the diversity of metabolites in different coarse cereals, we performed widely targeted metabolic profiling analyses of six popular coarse cereals, millet, coix, buckwheat, quinoa, oat, and grain sorghum, of which 768 metabolites are identified. Moreover, quinoa and buckwheat showed significantly different metabolomic profiles compared with other coarse cereals. Analysis of the accumulation patterns of common nutritional metabolites among six coarse cereals, we found that the accumulation of carbohydrates follows a conserved pattern in the six coarse cereals, while those of amino acids, vitamins, flavonoids, and lipids were complementary. Furthermore, the species-specific metabolites in each coarse cereal were identified, and the neighbor-joining tree for the six coarse cereals was constructed based on the metabolome data. Since sorghum contains more species-specific metabolites and occupies a unique position on the neighbor-joining tree, the metabolite differences between grain sorghum 654 and sweet sorghum LTR108 were finally compared specifically, revealing that LTR108 contained more flavonoids and had higher antioxidant activity than 654. Our work supports an overview understanding of nutrient value in different coarse cereals, which provides the metabolomic evidence for the healthy diet. Additionally, the superior antioxidant activity of sweet sorghum provides clues for its targeted uses.

Effects of morphology and rheology of starch nanoparticles prepared from various coarse cereals on emulsifying ability

Starch nanoparticles (SNPs) were prepared by rapid nanoprecipitation using coarse cereal starches, including quinoa, waxy proso millet, non-waxy proso millet, and maize starches (amylose content, 2.20 %–13.96 %). Morphology and dynamic light scattering analyses showed that the particle size of SNPs ranged from 190.35 nm to 310.25 nm. Ultrasonic pretreatment reduced the particle size. Quinoa and waxy proso millet SNPs swelled to flocculent, whereas non-waxy proso millet and maize SNPs showed spherical particles. Quinoa and waxy proso millet SNPs did not completely nucleate after nanoprecipitation. Quinoa SNPs formed a stable network in water, showing great viscoelastic behavior with higher G′ and G′′. Subsequently, the SNPs suspensions were used to prepare emulsions. The quinoa SNPs-based Pickering emulsion was the smallest and most uniform (12.96 μm), with great stability. These results suggested that the quinoa SNPs suspension with a stable network could be used as an efficient emulsifier for stabilizing Pickering emulsions.

In Search of the Determinants of Dairy Production in an Emerging Market

In view of the growing importance of the dairy sector, especially for the livelihood of the rural population and the increasing demand for milk by the Indian population, higher yield of milk animals and milk availability have become the focal point of attention. This study aims to identify the determinants of dairy production in India by examining the relationship of crossbreed and buffalo populations with the quantity of milk production across different Indian states. Fifteen major Indian states were included in this study and relevant secondary data from 2001 to 2019 was taken for analysis. The sources of statistical data are BAHS (GOI), Statistical abstract, NDDB website, etc. Coarse cereal, fodder crops, grazing land, artificial insemination and veterinary services have an influence on the total milk production of the Indian states, and the significance of these impacts were tested by pooled OLS regression analysis. Crossbreed cows, indigenous cows, buffalo and goat populations were tested with the Spearman’s rho correlation test, and these variables were also found to have a positive correlation with the quantity of total milk production (TMP). The study was further extended to compare the population strength of the crossbreed and buffalo, their growth rate and ultimately the status of average milk production across the major Indian states during the period 2001 to 2019. Uttar Pradesh, Rajasthan, Gujarat, Punjab and Andhra Pradesh were found to have healthy production of milk. However, Bihar and Rajasthan were found as promising states. States like Orissa, Kerala, Uttarakhand and West Bengal were found to have certain points of concern.

Foodgrains Production in India: Trend and Decompositions Analysis

The present study was undertaken to analyze the trends in area, production and productivity of major food grain crops for the period 1995-96 to 2015-16 obtaining data from the Directorate of Economics and Statistics, Ministry of Agriculture, Government of India, New Delhi. Foodgrains are cultivated in India in an area of 123.22 million hectares with a production of 251.57 million tonnes. The results of the study revealed that the food grains production in the country has increased at a rate of 1.73 per cent per annum which was contributed by negligible increase in area and productivity during the study period. The production-wise scenario of foodgrains has indicated that, major cereals like rice, wheat and maize showed consistent improvement, but pulses production was not consistent. As a coarse cereal, maize stands in an important position next to rice and wheat, due to its multiple uses with yield increases from about 1595 Kg per ha during 1995-96 to 2563 Kg per ha during 20015-16. The computed growth trend for total food grains in India was positive (0.008) and significant (P<0.01) for production and growth for productivity was positive (0.006) and significant (P<0.01).

Dietary Supplementation of Foxtail Millet Ameliorates Colitis-Associated Colorectal Cancer in Mice via Activation of Gut Receptors and Suppression of the STAT3 Pathway.

Coarse cereal intake has been reported to be associated with reduced risk of colorectal cancer. However, evidence from intervention studies is absent and the molecular basis of this phenomenon remains largely unexplored. This study sought to investigate the effects of foxtail millet and rice, two common staple grains in Asia, on the progression of colitis-associated colorectal cancer (CAC) and define the mechanism involved. In total, 40 BALB/c mice were randomized into four groups. The Normal and azoxymethane/dextran sodium sulfate (AOM/DSS) groups were supplied with an AIN-93G diet, while the millet- and rice-treated groups were supplied with a modified AIN-93G diet. Compared to the AOM/DSS-induced CAC mice supplemented with rice, an increased survival rate, suppressed tumor burden, and reduced disease activity index were observed in the millet-treated group. The levels of IL-6 and IL-17 were decreased in the millet-treated group compared to both the AOM/DSS and AOM/DSS + rice groups. Millet treatment inhibited the phosphorylation of STAT3 and the related signaling proteins involved in cell proliferation, survival and angiogenesis. These beneficial effects were mediated by the activation of gut receptors AHR and GPCRs via the microbial metabolites (indole derivates and short-chain fatty acids) of foxtail millet. Moreover, millet-treatment increased the abundance of Bifidobacterium and Bacteroidales_S24-7 compared to the rice-treated mice. This study could help researchers to develop better dietary patterns that work against inflammatory bowel disease (IBD) and for CAC patients.

Barley diseases and their management- an Indian perspective

Barley is an important coarse cereal, cultivated in Rabi season, particularly in the states of Uttar Pradesh, Rajasthan, Madhya Pradesh, Bihar, Punjab, Haryana, Himachal Pradesh and Jammu & Kashmir. Currently, it covers an area of about 0.66 million hectares under rainfed and irrigated crop. Seventy percent produce is used for cattle and poultry feed, 25% in industries for manufacturing malt and malt extracts and rest 5% for human consumption. The straw is also used for animal feed, bedding and to cover roofs of houses. Barley grains demand is increasing continuously because of its various uses and high nutritive value. Therefore, a substantial yield gain will be needed over the next several decades. A number of biotic and abiotic factors pose a challenge to increase production of barley. Barley diseases prominently rusts, net blotch, spot blotch, Septoria speckled leaf blotch, stripe disease, powdery mildew, barley yellow dwarf, and molya disease are the major biotic constraints in enhancing the barley grain production. Other diseases like black point and smuts, are important from the industrial point of view because these deteriorate the quality of malt and beer. This review seeks to provide an overview of different barley diseases and their management. Doi.org/10.25174/2249-4065/2018/83844

Effects of four coarse cereals on blood glucose levels in rats with STZ-induced hyperglycemia

ABSTRACT This study aims to compare the protection effects of different varieties of oats, buckwheat, barley and foxtail millet against streptozotocin (STZ)-induced hyperglycemia in rats. Eight varieties of each coarse cereal were selected from the main production areas of China. The contents of resistant starch in oats, buckwheat, barley and foxtail millet were 31.41 ± 5.16%, 31.55 ± 10.34%, 19.77 ± 4.13% and 43.54 ± 9.01%, respectively. These four coarse cereals were added to diets of rats at 15%. The results showed that five varieties of buckwheat (Chitianqiao No. 1, Xinong No. 9976, Taixing, Chuanqiao No. 1 and Heifeng No. 1) significantly decreased the blood glucose levels (P < .05) with the highest total phenolic content. Two varieties of barley (Supi No. 3 and Zangqing No. 2000) and two varieties of foxtail millet (Yugu No. 18 and Ansai) significantly reduced the blood glucose levels in vivo (P < .05).

A R2R3-MYB transcription factor gene, FtMYB13, from Tartary buckwheat improves salt/drought tolerance in Arabidopsis

Abiotic stress causes various negative impacts on plants, such as water loss, reactive oxygen species (ROS) accumulation and decreased photosynthesis. R2R3-MYB transcription factors (TFs) play crucial roles in the response of plants to abiotic stress. However, their functions in Tartary buckwheat, a strongly abiotic and resistant coarse cereal, haven't been fully investigated. In this paper, we report that a R2R3-MYB from Tartary buckwheat, FtMYB13, is not an activator of transcriptional activity but is located in the nucleus. Moreover, compared to the wild type (WT), transgenic Arabidopsis overexpressing FtMYB13 had a lower sensitivity to ABA and caused improved drought/salt tolerance, which was attributed to the higher proline content, greater photosynthetic efficiency, higher transcript abundance of some stress-related genes and the smaller amount of reactive oxygen species (ROS) and malondialdehyde (MDA) in the transgenic lines compared to WT. Consequently, our work indicates that FtMYB13 is involved in mediating plant responses to ABA, as well as salt and drought.

Overexpression of Fagopyrum tataricum FtbHLH2 enhances tolerance to cold stress in transgenic Arabidopsis

bHLH transcription factors play important roles in the abiotic stress response in plants, but their characteristics and functions in Tartary buckwheat (Fagopyrum tataricum), a traditional coarse cereal with a strong stress tolerance, haven't been sufficiently studied. Here, we found that the expression of a bHLH gene, FtbHLH2, was induced significantly by cold treatments in Tartary buckwheat seedlings. Subcellular localization indicated that FtbHLH2 localized in nucleus. Its overexpression in Arabidopsis increased tolerance to cold. The Arabidopsis plants overexpressing FtbHLH2 displayed higher root length and photosynthetic efficiency, and had lower malondialdehyde (MDA) and reactive oxygen species (ROS) after cold treatment compared to wild type (WT) plants. Meanwhile, the expression levels of some stress-related genes in transgenic plants were remarkably higher than that in wild type under normal and/or stress conditions. Furthermore, transgenic Arabidopsis lines with the FtbHLH2 promoter had higher GUS activity after cold stress. On the whole, the results suggest that FtbHLH2 may play a positive regulatory in cold stress of Tartary buckwheat.

Effects of explosion puffing on the nutritional composition and digestibility of grains

ABSTRACTThe objective of this study was to investigate the effects of explosion puffing on the nutritional composition, physical properties, and digestibility of grains including millet, barley, black rice, rice, glutinous rice, and wheat. Explosion puffing of grains resulted in the nutritional composition with higher total sugar content and lower moisture, starch and fat contents. Although the protein content scarcely changed comparing with the untreated grains, the solubility declined significantly. Moreover, explosion puffing could significantly improve the physical properties including the water absorption index, water solubility index and gelatinization degree. The in vitro digestion experiment was employed to investigate the influences of explosion puffing on the starch hydrolysis rate and free amino acid content, and it was indicated that the digestibility of the starch and protein in grains was highly improved. The results demonstrated that explosion puffing is a suitable technique to improve the physical properties and digestibility of grains, which provided the theoretic foundation for the use of explosion puffing technology in coarse cereal processing.

Gluten free approach in fat and sugar amended biscuits: A healthy concern for obese and diabetic individuals

Flour premix of copra meal 15% with germinated foxtail millet 85% blended with amaranth in 60:40 proportions to get gluten free, calorie deficient composite mix for biscuits. Attempts were made for obese and diabetics as target population with maltodextrin and acesulfame-k to find the extent of possible partial replacement of bakery fat and table sugar, respectively. It was found that substitution feasibility to 48% fat and 47% sugar lie within the acceptable limits of physical and sensorial properties comparable to control sample. Successful calorie reduction in fat and binary (fat and sugar) amended biscuits were achieved to 364.58 kcal/g and 315.97 kcal/g, respectively, as in comparison with control (471.01 kcal/g). Low anti-nutrients (phytate and tannin) and increased polyphenols in biscuits is a function of germinated foxtail millet. Acrylamide development also restricted to 0.193 µg/g in sugar replaced and 0.32 µg/g in sugar and fat replaced biscuits comparable to control (0.5 µg/g). Practical applications Copra as by-product from coconut industry is rich in fiber and protein but still restricted to as livestock feed till date. Its utilization for human is itself a novel approach. Standardized inclusion of copra meal to selected nutritious gluten free grains reveals a healthy budgetary option. Further, intentional substitution of dense calorie compounds (fat and sugar) with their calorie deficit counterpart to target obese and diabetic along with celiac is investigated. Owe to protein richness of selected coarse cereal varieties and copra, huge malnourished population may easily access pseudo cereal and coarse cereal based composite food with enhanced health attributes.

Multiple hybrid de novo genome assembly of finger millet, an orphan allotetraploid crop

Finger millet (Eleusine coracana (L.) Gaertn) is an important crop for food security because of its tolerance to drought, which is expected to be exacerbated by global climate changes. Nevertheless, it is often classified as an orphan/underutilized crop because of the paucity of scientific attention. Among several small millets, finger millet is considered as an excellent source of essential nutrient elements, such as iron and zinc; hence, it has potential as an alternate coarse cereal. However, high-quality genome sequence data of finger millet are currently not available. One of the major problems encountered in the genome assembly of this species was its polyploidy, which hampers genome assembly compared with a diploid genome. To overcome this problem, we sequenced its genome using diverse technologies with sufficient coverage and assembled it via a novel multiple hybrid assembly workflow that combines next-generation with single-molecule sequencing, followed by whole-genome optical mapping using the Bionano Irys® system. The total number of scaffolds was 1,897 with an N50 length >2.6 Mb and detection of 96% of the universal single-copy orthologs. The majority of the homeologs were assembled separately. This indicates that the proposed workflow is applicable to the assembly of other allotetraploid genomes.