Coal Tit Research Articles

Lifespan, lifetime reproductive performance and paternity loss of within-pair and extra-pair offspring in the coal tit Periparus ater

The hypothesis that females of socially monogamous species obtain indirect benefits (good or compatible genes) from extra-pair mating behaviour has received enormous attention but much less generally accepted support. Here we ask whether selection for adult survival and fecundity or sexual selection contribute to indirect selection of the extra-pair mating behaviour in socially monogamous coal tits (Periparus ater). We tracked locally recruited individuals with known paternity status through their lives predicting that the extra-pair offspring (EPO) would outperform the within-pair offspring (WPO). No differences between the WPO and EPO recruits were detected in lifespan or age of first reproduction. However, the male WPO had a higher lifetime number of broods and higher lifetime number of social offspring compared with male EPO recruits, while no such differences were evident for female recruits. Male EPO recruits did not compensate for their lower social reproductive success by higher fertilization success within their social pair bonds. Thus, our results do not support the idea that enhanced adult survival, fecundity or within-pair fertilization success are manifestations of the genetic benefits of extra-pair matings. But we emphasize that a crucial fitness component, the extra-pair fertilization success of male recruits, has yet

Sequential use of holes by birds breeding in a natural boreal forest in Mongolia

Capsule Wood Nuthatch Sitta europaea and Coal Tit Periparus ater had a high tendency to reuse nest-sites, and holes in living trees were more frequently reused than holes in dead trees. Aims Patterns of sequential nest-hole use are important in understanding the inter- and intraspecific relationships of hole-nesting birds. We studied the hole reuse at both guild and species level, and examined the characteristics of reused holes. Methods The fieldwork was carried out in a strictly protected area in Mongolia. Occupied nest-holes were searched for all hole-nesting species in two subsequent years. The characteristics of nest-holes and nest trees were recorded. Results Of 105 holes occupied in 2002, 40 (38%) were reused in the following year. The reuse rate for holes previously used by non-excavators was 48%, and subsequent occupation was almost exclusively by the same species. Reuse rates for previous woodpecker and Willow Tit Poecile montanus holes were 31% and 16%, respectively, and the subsequent users were more frequently a different species. Nuthatches and Coal Tits had the highest propensity to reuse holes. Holes located in living parts of trees were reused more often than those in dead wood. Conclusion The tendency to reuse holes could be influenced by the ability to excavate, the ability to compete for nest-sites, and the quality and abundance of preferred hole type of each bird species. The high reuse rate of certain holes would indicate good qualities and/or more limited availability of such holes. The more stable microclimate and greater protection from predation might account for the frequent reuse of holes in living wood.

Variation in the magnitude of sexual size dimorphism in nestling Coal Tits (Periparus ater)

The magnitude of sexual size dimorphism can be affected by sex differences in environmental sensitivity early in ontogeny that result in differential growth rates of male and female nestlings. Here, the larger sex might either be more sensitive because of higher food demands or less sensitive due to greater competitive ability. When environmental conditions deteriorate during the breeding season, this “environmental stress” hypothesis predicts differential seasonal declines in the performance of male and female offspring. Based on a sample of molecularly sexed Coal Tit (Periparus ater) nestlings from 2 years, we investigated sexual size dimorphism in body mass, condition (i.e. size-corrected mass), tarsus and wing length and whether its magnitude changed from early to late broods. Male offspring were heavier, larger (in terms of tarsus and wing length) and had higher size-corrected mass than their female nest mates (the same was evident in adult breeders). In 2002 (the year with the longer effective breeding season), body mass and condition declined with progressing hatching date and this effect was significantly more pronounced in male than in female nestlings. There was also a seasonal decline in male wing length, while female wing length remained relatively constant, which resulted in males having shorter wings than females in late broods. Tarsus length was unaffected by time of breeding, except that the difference between males and females was relatively smaller in late (i.e. second) broods in 2002. While these results are in accordance with the idea of an increased environmental sensitivity of the larger males, confounding effects of sex-differential hatching order cannot be ruled out.

Between-species correlations in the number of migrants at Ulmethöchi (Switzerland)

We analysed the number of autumn migrants at a bird ringing station over 41 years in the Jura mountains of Switzerland. For 12 irruptive or potentially irruptive bird species, the correlations between their numbers per year were calculated and the species were clustered accordingly. We found high correlations in the number of migrants between the Coal Tit Periparus ater, Great Tit Parus major and Blue Tit Cyanistes caeruleus. Most correlations of passage number between species pairs changed dramatically over time. Only Blue Tit and Coal Tit showed continuously high correlation in this respect. The variation and changes over time in between-species correlations in the number of migrants needs more attention.

Hyper-dispersed cache distributions reduce pilferage: a laboratory study

L. H. Male and T. V. Smulders (correspondence), Newcastle University, Centre for Behaviour and Evolution, Henry Wellcome Building,Framlington Place, Newcastle upon Tyne, NE21 4HH, UK. E-mail: tom.smulders@ncl.ac.ukMany animals use hoarding as a long-term strategy to ensure a food supply at times of shortage. We suggest that long-term scatter hoarders, whose caches are vulnerable to potentially high pilferage, should hoard in ways to reduce cache loss.This could be achieved by manipulating the density and dispersal patterns of caches to reduce the foraging efficiency ofpilferers. This study explores the effect of distribution patterns on cache loss in the laboratory. We recorded the discoveryof food items in different dispersal patterns by two bird species: coal tits Periparus ater (a hoarder) and great tits Parusmajor (a non-hoarder). Hyper-dispersed distributions reduced foraging efficiency because both species used systematiclocal search patterns. This study shows that hyper-dispersed distributions would be advantageous to hoarding animals toreduce cache loss.

Food diversity and niche-overlap of sympatric tits (Great Tit, Parus major, Blue Tit, Cyanistes caeruleus and Coal Tit Periparus ater) in the Hyrcanian Plain forests

This study was carried out between early summer 1998 and late summer 1999 in the Hyrcanian Plain forests, the southern Caspian Sea woodlands. In total, the gizzard contents of 241 specimens of the Great Tit (Parus major Linnaeus, 1758), Blue Tit (Cyanistes caeruleus Linnaeus, 1758), and Coal Tit (Periparus ater (Linnaeus, 1758)) were collected so that the percentages of grit, plants and animal materials could be estimated and the plant and invertebrate species consumed identified. The data were tested by a series of Mann-Whitney U, ANOVA and Kruskal-Wallis tests. To survey niche overlap of food materials between species and season, the symmetrical overlap index developed by Pianka (1973) was used. The most important invertebrates (maximum of percentage per season) in the diet of P. major were Coleoptera (36.5%) and Lepidoptera (33.6%), for C. caeruleus Coleoptera (34.5%) and Araneae (31.2%), and for Pe. ater Coleoptera (38.2%) and Araneae (35.6%). The most important plants consumed by P. major were Sorghum halepense (68.7%) and Ficus carica (65.6%), by C. caeruleus were F. carica (67.5%) and Alnus glutinosa (39.4%), and by Pe. ater were Lonicera spp. (82.9%). There was a greater degree of overlap and competition for animal food, but plant feeding overlap among the three tit species was low. Two species, P. major and C. caeruleus, showed high feeding overlap for animal items (0.92), whereas Pe. ater and C. caeruleus had no significant feeding overlap. The Coal Tit had a greater tendency to forage for vegetable matter and P. major had a greater tendency to forage for animal matter. Analysis of gizzard contents of P. major and Pe. ater showed that, despite the high degree of feeding overlap, the extent of plant materials consumed indicated no significant competition between the two species. Because Pe. ater is present mostly in autumn and winter, this study cannot establish any degree of competition and food-niche overlap during the breeding season between this and other tit species.

Ousting of the Common Redstart (Aves: Turdidae: Phoenicurus phoenicurus) from its nests by the bumblebee Bombus niveatus vorticosus (Hymenoptera: Apidae)

During a study of bird nesting in SW. Anatolia, 125 man-made nest boxes have been installed, 3 m high, in the trees. These nest boxes have been occupied by several bird species (Phoenicurus phoenicurus, Parus major, Parus ater, Sitta krueperi, Certhia brachydactyla). Of the 48 Redstart nests, 19 (40 %) had been successfully invaded by the bumblebee Bombus niveatus vorticosus. This invasion occurred at different times in the nest building cycle: - during the building of the nest, - during the incubation or - after the hatching of the young. Once installed in the nest of the Redstart, the bumblebee does not directly attack the bird, but disturbs it by continuously rearranging the nesting material and by covering the bird’s brood. Eventually, the bird deserts its nest and brood. The Redstart does not display any agonistic behaviour towards the bumblebee. Nests of other bird species are never invaded.

Recent changes in bird populations in British broadleaved woodland

This paper presents estimated national and regional changes in numbers of breeding woodland birds in predominantly broadleaved woodland in Britain between the mid 1980s and 2003–04, derived from the Repeat Woodland Bird Survey (RWBS). A total of 405 sites across Britain for which historical data on bird populations were available were re‐surveyed by BTO and RSPB fieldworkers during the RWBS. Changes were estimated separately for sites surveyed by the RSPB (n = 252) and BTO (n = 153) because of differences between the two groups in the field methods used in the original surveys. Changes between the 1960s and 1970s and 2003–04 were also estimated with the BTO data set only. The BTO and RSPB sites provide geographically complementary coverage of Britain and together provide much more widespread coverage than did the Common Bird Census (CBC), the former national monitoring scheme. Nationally, between the 1980s and 2003–04, six species declined significantly and three increased significantly based on the RSPB data, whereas eight declined and 13 increased according to the BTO data, with good correspondence between the changes recorded by the two data sets (only seven of 34 species showing trends differing in direction). Eight species showed large national declines (> 25%) according to both data sets: Garden Warbler Sylvia borin, Lesser Redpoll Carduelis cabaret, Lesser Spotted Woodpecker Dendrocopos minor, Spotted Flycatcher Muscicapa striata, Tree Pipit Anthus trivialis, Willow Tit Poecile montanus, Willow Warbler Phylloscopus trochilus and Wood Warbler P. sibilatrix. Eleven species showed large national increases (> 25%) in both data sets: Blackcap Sylvia atricapilla, Blue Tit Cyanistes caeruleus, Common Chiffchaff Phylloscopus collybita, Coal Tit Periparus ater, Great Spotted Woodpecker Dendrocopos major, Great Tit Parus major, Green Woodpecker Picus viridis, Goldcrest Regulus regulus, European Robin Erithacus rubecula, Eurasian Treecreeper Certhia familaris and Winter Wren Troglodytes troglodytes. Analysis of the longer‐term changes using the BTO data set showed significant declines between the 1960s and 2003–04 in seven species and increases in 12 species. The large declines detected by the national monitoring schemes (CBC/BBS) for Lesser Spotted Woodpecker, Lesser Redpoll, Spotted Flycatcher, Tree Pipit, Willow Tit, Willow Warbler and Wood Warbler were all confirmed by the RWBS but the declines of the Common Bullfinch Pyrrhula pyrrhula, Marsh Tit Poecile palustris and Mistle Thrush Turdus viscivorus were not clearly supported. The declines detected by the RWBS for Garden Warbler, Pied Flycatcher Ficedula hypoleuca and Common Redstart Phoenicurus phoenicurus were not evident from the national monitoring schemes. Most of the increases detected by the monitoring schemes were confirmed by the RWBS. However, the large increases detected by the RWBS for Coal Tit, Goldcrest, Great Tit and Eurasian Treecreeper were unexpected. All long‐distance migrants have declined whilst medium‐distance migrants (Blackcap and Common Chiffchaff) have increased. Also, rare and specialist woodland species have fared less well than common and generalist species.

Male age predicts extrapair and total fertilization success in the socially monogamous coal tit

An important step to a better understanding of the adaptive significance of extrapair mating behavior in socially monogamous species is to uncover the identity of extrapair sires (EPS). Here, we used a combination of multilocus DNA fingerprinting and microsatellite analysis to identify EPS in the socially monogamous coal tit (Periparus ater), a passerine bird with high rates of extrapair paternity. We then analyzed how fertilization success was related to male age in 2 consecutive first brood periods based on knowledge of the exact age of the majority of territorial males. EPS were significantly older compared with the males they cuckolded. Furthermore, extrapair and, as a consequence, also total fertilization success were positively related to male age, while within-pair success was not. Interestingly, fertilization success did not increase linearly with male age but leveled off for older age classes and was most parsimoniously described by the inverse term of male age. Results were consistent over the 2 years, while the demography of the study population differed with respect to the age distribution of territorial males. Furthermore, we also show that individual males increased their extrapair fertilization (EPF) success with age indicating that cross-sectional analyses were not confounded by cohort effects. Together with the results from other species, these findings suggest that male age (or a strong correlate thereof) is a major determinant of EPF success in several socially monogamous bird species.

The effects of latitude and day length on fattening strategies of wintering coal tits Periparus ater (L.): a field study and aviary experiment

1. Cyclic daily fattening routines are very common in wintering small wild birds, and are thought to be the consequence of a trade-off between different environmental and state-dependent factors. According to theory, these trajectories should range from accelerated (i.e. mass increases exponentially towards dusk) when mass-dependent predation costs are the most important cause of mortality risk, to decelerated (i.e. the rate of mass gain is highest at dawn and decreases afterward) when starvation is the greater risk. 2. We examine if geographically separate populations of coal tits, wintering in Scotland and central Spain under contrasting photoperiods, show differences in their strategies of daily mass regulation. We describe population differences in wild birds under natural conditions, and experimentally search for interpopulation variation in diurnal body mass increase under common, manipulated, photoperiod conditions (LD 9 : 15 h vs. 7 : 17 h), controlling for temperature, food availability, predator pressure and foraging arena. 3. Winter diurnal mass gain of wild coal tits was more delayed towards the latter part of the daylight period in central Spain (i.e. the locality with longer winter days) than in Scotland. In both localities, the pattern was linked to the average mass at dawn, with mass increasing more rapidly in lighter birds. However, under the controlled photoperiod situation the pattern of daily mass gain was similar in both populations. Diurnal body mass gain was more accelerated at the end of the day, and the increase in body mass in the first hour of the day was considerably lower under the long (9 h) than under the short (7 h) photoperiod in both populations. 4. Wintering coal tits show patterns of mass gain through the day that are compatible with current theories of the costs and benefits of fat storage, with birds at lower latitudes (with longer winter days) having a greater tendency to delay mass gain until late in the day. The experimental study revealed that these patterns are plastic, with birds responding directly to the photoperiod that they experience, suggesting that they are continually making fine-scale adjustments to energy reserves on the basis of both inherent (e.g. state-dependent) and extrinsic cues.

Importance of Sitka Spruce Picea sitchensis seed and garden bird-feeders for Siskins Carduelis spinus and Coal Tits Periparus ater

Capsule Siskins and Coal Tits were present in gardens more often in years of poor Sitka Spruce cone crops, with synchrony across the UK. Aims To examine how the relative abundances of Siskins and Coal Tits in a garden in western Scotland varied annually with the size of the spruce cone crop, and to determine whether this pattern was similar nationally. Methods A ten-year mist-netting data set from Tarbet, Argyll and Bute was used to test the hypothesis that some species utilize supplementary food in gardens more often in years with poor cone crops than in years when crops are large. Annual peaks in the relative abundances of four species (two that ate conifer seed and two that did not) in Tarbet were correlated with a Sitka Spruce masting index. BTO Garden BirdWatch data from six regions of the UK were correlated with the Tarbet data, the cone index and each other to determine the extent of synchrony. Results Both Siskins and Coal Tits were present more often in the Tarbet garden in years with poor cone crops, with relative abundances significantly negatively correlated with cone crop for both species (r = −0.739 and −0.832, respectively). Correlations between regions using BTO data showed abundances of species in gardens to be broadly similar across the UK. Independent scores of cone abundance in Kielder and Galloway were correlated. Correlations between BTO data, cone index and Tarbet garden data were mostly high and almost all in the same direction, also indicating a degree of synchrony in cone production across the UK. Conclusion Both Siskins and Coal Tits appear to be influenced by changes in cone abundance, switching to feed on supplementary food in gardens more often in years with few cones than in mast years. While such a relationship has been shown previously for Siskins, for Coal Tits this is a novel result.

Spatial memory of four species of parus: Performance in an open-field analogue of a radial maze

The performance of four species of tit (marsh tit, Parus palustris; coal tit, P. ater; blue tit, P. caeruleus; and great tit, P. major) and one finch (greenfinch, Carduelis choris) was compared in ...

Complex Responses of Songbirds to Soil Acidification of Managed Beech Forests in Central Europe

Many forest ecosystems have been extensively degraded by acidifying pollutants over the last decades. There is also widespread concern about population decline of a number of songbirds due to acidification-driven calcium limitation. We hypothesize that the carrying capacity of forests decreases with reduced soil calcium content. We analyzed songbird assemblage/habitat relationships by territory mapping at 30 sites in a total of five managed beech forests. The sites harbored 38 songbird species. A canonical correspondence analysis showed that soil acidification, represented by soil calcium content, had a significant influence on the species assemblages of the 14 most frequent songbirds. The vertical complexity of the canopy additionally influenced the assemblages. Multiple regressions revealed that the density of the territories of song thrush (Turdus philomelos) and nuthatch (Sitta europaea) were positively correlated to calcium content. Blackbirds (Turdus merula) seemed to compensate for the negative effect of acidification by selecting breeding sites close to forest edges to forage in less acidified agricultural habitats. In contrast, the territorial density of robin (Erithacus rubecula), chaffinch (Phylloscopus collybita) and coal tit (Parus ater) increased with increasing soil acidification. Contrary to our hypothesis, the overall carrying capacity of songbird was not reduced in forests with acidified soils. However, the nesting success of song thrush and blackbird, as determined by the number of fledglings, was higher in the forest with the highest calcium content when compared to the forest with the lowest calcium content. We conclude that some species are severely affected by acidification, whereas others seem to have evolved successful strategies to meet their calcium demand even in acidified habitats.

Calibration of a molecular clock in tits (Paridae)—Do nucleotide substitution rates of mitochondrial genes deviate from the 2% rule?

The ongoing debate on the reliability of avian molecular clocks is actually based on only a small number of calibrations carried out under different assumptions with respect to the choice and constraints of calibration points or to the use of substitution models. In this study, we provide substitution rate estimates for two mitochondrial genes, cytochrome b and the control region, and age estimates for lineage splits within four subgenera of tits (Paridae: Parus, Cyanistes, Poecile and Periparus). Overall sequence divergence between cytochrome b lineages covers a range of 0.4–1.8% per million years and is thus consistent with the frequently adopted approximation for a sequence divergence between avian lineages of 1.6–2% per my. Overall rate variation is high and encompasses the 2% value in a 95% CI for model corrected data. Mean rate estimates for cytochrome b range between 1.9 and 8.9 × 10 −3 substitutions per site per lineage. Local rates differ significantly between taxonomic levels with lowest estimates for haplotype lineages. At the population/subspecies level mean sequence divergence between lineages matches the 2% rule best for most cytochrome b datasets (1.5–1.9% per my) with maximum estimates for small isolated populations like those of the Canarian P. teneriffae complex (up to 3.9% per my). Overall rate estimates for the control region range at similar values like those for cytochrome b (2.7–8.8 × 10 −3, 0.5–1.8% per my), however, within some subgenera mean rates are higher than those for cytochrome b for uncorrected sequence data. The lowest rates for both genes were calculated for coal tits of subgenus Periparus (0.04–0.6% per my). Model-corrected sequence data tend to result in higher rate estimates than uncorrected data. Increase of the gamma shape parameter goes along with a significant decrease of rate and partly age estimates, too. Divergence times for earliest deep splits within tit subgenera Periparus and Parus were dated to the mid Miocene at 10–14 my bp. Most recent splits between east and west Palearctic taxa of blue, willow and great tits were dated to the Pliocene/Pleistocene boundary with the earliest estimates based on model-corrected trees. Relaxation of the Messinian calibration point leads to more recent divergence times for North African coal and blue tit populations during the mid Pliocene. Despite a relatively broad age constraint for the split between Nearctic and Palearctic Poecile due to the Pliocene re-opening of the Bering Strait, the split between chickadees and willow tits is dated considerably earlier than in former studies to the upper bound of the age constraint at 7.4 my BP.

Effects of the winter beechmast crop on bird occurrence in British gardens

Capsule Woodland birds were significantly less likely to occur in gardens in years of high beechmast crop. Aim To test the hypothesis that woodland species that feed on beechmast will have significantly lower occurrence rates at garden feeders in mast years. Methods Weekly winter occurrence rates at garden feeders between 1970/71 and 1999/2000 for 40 species were analysed in relation to annual beechmast abundance, classified into low, medium and high years. A repeated-measures logistic regression model was used to assess whether beechmast abundance explained further significant variation additional to underlying seasonal and annual trends. Results Seven species that commonly feed on beechmast showed significantly lower occurrence in gardens in years of highest beechmast abundance: Great Spotted Woodpecker Dendrocopos major, Woodpigeon Columba palumbus, Great Tit Parus major, Coal Tit Periparus ater, Nuthatch Sitta europaea, Jay Garrulus glandarius and Chaffinch Fringilla coelebs. Blackbird Turdus merula and Siskin Carduelis spinus, which showed similar significant patterns, are likely to take beechmast as elements of their diet. Pied Wagtail Motacilla alba was the only insectivorous species to show significant effects, but occurrence was lowest in years of intermediate beechmast abundance. For the latter species, this may have been due to confounding effects of temperature, but there were no such confounding effects of either temperature, or the number of bird feeders provided in gardens, for the other nine species. Conclusion Use of artificial food sources by birds in gardens is influenced by resources in the surrounding countryside, suggesting that food provided in gardens may play a significant part in the population dynamics of these species, that population monitoring without consideration of the garden habitat may be deficient, and that volunteer-based garden bird recording may provide data that can be used as an indicator of changes in the wider countryside.

Memory for food caches: not just for retrieval

Many animals use hoarding as a long-term strategy to ensure a food supply at times of shortage. Hoarders employ strategies that enhance their ability to relocate caches such as remembering where caches are located. Long-term scatterhoarders, whose caches have potentially high pilferage rates, should also hoard in a way to reduce potential cache pilferers’ ability to find caches. Previous studies have demonstrated that this could be achieved by hyperdispersing caches to reduce the foraging efficiency of pilferers. This study investigates whether coal tits (Parusater) indeed place their caches away from existing ones. In our experiment, birds hoarded food in 3 conditions: when caches from a previous storage session were still present, when caches from a previous storage session were not present anymore because the bird had retrieved them, and when caches from a previous storage session had been removed by the experimenter. We show that coal tits hoard away from existing caches and that they do not use cues from extant caches to do this. This evidence is consistent with the use of memory for the locations of previous caches when deciding where to place new caches. This finding has important implications for our understanding of the selective pressures that have shaped spatial memory in food-hoarding birds. Key words: cache dispersal, coal tits, cognitive ecology, food-hoarding birds, Parus ater. [Behav Ecol]

Polyandry in coal tits Parus ater: fitness consequences of putting eggs into multiple genetic baskets

Females of many species mate with multiple males within a single reproductive cycle. One hypothesis to explain polyandry postulates that females benefit from increasing within-brood genetic diversity. Two mechanisms may render sire genetic diversity beneficial for females, genetic bet-hedging vs. non-bet-hedging. We analysed whether females of the socially monogamous coal tit (Parus ater) benefit via either of these mechanisms when engaging in extra-pair (i.e. polyandrous) mating. To obtain a measure of within-brood genetic diversity as a function of paternal genetic contributions, we calculated a sire diversity index based on the established Shannon-Wiener Index. In 246 broods from two consecutive years, sire genetic diversity had no effect on either the mean or the variance in brood fitness measured as offspring recruitment within 4 years after birth. The hypothesis that benefits of increasing sire diversity contribute to selection for female extra-pair mating behaviour in P. ater was therefore not supported.

Memory decay and cache site preferences in hoarding coal tits — a laboratory study

Summary Many animals use hoarding as a long-term strategy to ensure a food supply at times of shortage. This study explores the respective roles of memory and site preferences in cache recovery by coal tits (Periparus ater). We compared the retrieval accuracy or foraging efficiency of the cacher itself (‘caching coal tit’), a naive conspecific (‘pilfering coal tit’) and a non-hoarding heterospecific (‘pilfering great tit’; Parus major) after six different retention intervals. Our experiment shows that the persistence of the coal tits’ memory is up to 4 weeks in the laboratory. Species specific storage and foraging site preferences enhance cache recovery after longer intervals. We find no evidence for individual-specific preferences. Pilfering great tits are capable of learning the coal tits’ hoarding preferences. This ability may affect coal tits’ hoarding behaviour in more natural conditions as they frequently forage in mixed flocks with great tits.

Haemosporidian infection in passerine birds from Lower Saxony

Blood samples from 94 coal tits (Parus ater), 56 great tits (Parus major) and 219 pied flycatchers (Ficedula hypoleuca), caught between 1993 and 2002 at two localities in Lower Saxony, Germany, were examined for haemosporidian infection by parasite-specific polymerase chain reaction (PCR). A simple PCR targeting the 18 SSU rRNA gene of the parasites was used for rapid screening of the samples and generated a total infection prevalence of 20.6% (76/369): 6.8% (n = 15) of the pied flycatchers, 19.1% (n = 18) of the coal tits and 76.8% (n = 43) of the great tits were infected. The positive specimens were re-examined by a cytochrome b gene-directed nested PCR producing significantly longer DNA fragments (approx. 520 bp) that were sequenced and analysed against GenBank-deposited nucleotide sequences. In various numbers (once to 30 times), a total of 13 parasitic DNA sequences differing from 2.9 to 8.5% (13–45 nucleotides) were demonstrated in the three bird species. Due to similarities of 98–100% with GenBank entries, 11 sequences could be assigned to Plasmodium sp. and two to the genus Haemoproteus. In summary, 57 birds were infected with Plasmodium and 19 with Haemoproteus, corresponding to 15.4 and 5.1% of all birds examined, and to 75 and 25% of all birds tested positive. As the only defined species, Haemoproteus majoris was identified in 17 great tits.

First record of Diplotriaena henryi Blanc, 1919 from the coal tit, Parus ater with new report from the great tit, Parus major in the Middle East

Abstract Seven males and thirteen female Diplotriaena henryi Blanc 1919 were collected from body cavities (heart, sternum, and chest) of tits (one Parus major and three Parus ater) in plain woodland of Noor, in Northern Iran.