Coingestion Of Protein Research Articles

Formation of adducts during digestion triggered dietary protein for alleviating cytotoxicity of 2-tert-butyl-1,4-benzoquinone

2-tert-butyl-1,4-benzoquinone (TBBQ), a degradation product of lipid antioxidant Tert-Butylhydroquinone (TBHQ), is a new hazardous compound in foods. This study investigated whether co-ingestion of dietary protein and TBBQ can alleviate the toxicity of TBBQ. The results indicated that soy protein isolate, whey protein isolate, and rice protein significantly reduced the residual amount of TBBQ during simulated gastrointestinal digestion. This result was attributed to the excellent elimination capacity of the released amino acids for TBBQ through formation of adducts. Among 20 amino acids, histidine, lysine, glycine, and cysteine showed better elimination capacity for TBBQ; they can eliminate 92.1%, 89.4%, 86.1%, and almost 100%, respectively, in 5 min at pH 8.0. Further study indicated that amino acids with lower ionization constant exhibited greater TBBQ elimination capacity. In addition, incubation of the cells with 50 μM TBBQ for 12 h decreased the cell viability to 28.95 ± 3.25%; while amino acids intervention was involved in the alleviation of TBBQ cytotoxicity via decreasing ROS. Particularly, cysteine showed 100 times more TBBQ detoxifying capacity than other amino acids. This work could provide a theoretical basis for the potential application of amino acids for detoxifying TBBQ in the food industry.

Effects of co-ingesting glucose and whey protein on blood glucose, plasma insulin and glucagon concentrations, and gastric emptying, in older men with and without type 2 diabetes.

To investigate whether co-ingestion of dietary protein with, or before, carbohydrate may be a useful strategy to reduce postprandial hyperglycaemia in older men with type 2 diabetes (T2D). Blood glucose, plasma insulin and glucagon concentrations were measured for 180 minutes following ingestion of a drink containing 30 g of glucose (G; 120 kcal), 30 g of whey protein (120 kcal), 30 g of glucose plus 30 g of whey protein (GP; 240 kcal), or control (~2 kcal) in older men with T2D (n=10, 77 ± 1 years; 31 ± 1.7 kg/m2 ) and without T2D (n=10, 78 ± 2 years; 27 ± 1.4 kg/m2 ). Mixed model analysis was used. GP versus G markedly reduced the increase in blood glucose concentrations (P < .001) and had a synergistic effect on the increase in insulin concentrations (P < .001), in men both with and without T2D. Glucose concentrations were higher in men with T2D compared with those without T2D, whereas insulin and glucagon concentrations were largely unaffected by the presence of T2D. Gastric emptying was faster in men with T2D than in those without T2D. The ability of whey protein to reduce carbohydrate-induced, postprandial hyperglycaemia is retained in older men with T2D compared with those without T2D, and whey protein supplementation may be a useful strategy in the prevention and management of T2D in older people.

Understanding the effects of nutrition and post-exercise nutrition on skeletal muscle protein turnover: Insights from stable isotope studies

Understanding the effects of nutrition and post-exercise nutrition on skeletal muscle protein turnover: Insights from stable isotope studies

Coingestion of Carbohydrate and Protein on Muscle Glycogen Synthesis after Exercise: A Meta-analysis.

Evidence suggests that carbohydrate and protein (CHO-PRO) ingestion after exercise enhances muscle glycogen repletion to a greater extent than carbohydrate (CHO) alone. However, there is no consensus at this point, and results across studies are mixed, which may be attributable to differences in energy content and carbohydrate intake relative to body mass consumed after exercise. The purpose of this study was determine the overall effects of CHO-PRO and the independent effects of energy and relative carbohydrate content of CHO-PRO supplementation on postexercise muscle glycogen synthesis compared with CHO alone. Meta-analysis was conducted on crossover studies assessing the influence of CHO-PRO compared with CHO alone on postexercise muscle glycogen synthesis. Studies were identified in a systematic review from PubMed and Cochrane Library databases. Data are presented as effect size (95% confidence interval [CI]) using Hedges' g. Subgroup analyses were conducted to evaluate effects of isocaloric and nonisocaloric energy content and dichotomized by median relative carbohydrate (high, ≥0.8 g·kg-1⋅h-1; low, <0.8 g·kg-1⋅h-1) content on glycogen synthesis. Twenty studies were included in the analysis. CHO-PRO had no overall effect on glycogen synthesis (0.13, 95% CI = -0.04 to 0.29) compared with CHO. Subgroup analysis found that CHO-PRO had a positive effect (0.26, 95% CI = 0.04-0.49) on glycogen synthesis when the combined intervention provided more energy than CHO. Glycogen synthesis was not significant (-0.05, 95% CI = -0.23 to 0.13) in CHO-PRO compared with CON when matched for energy content. There was no statistical difference of CHO-PRO on glycogen synthesis in high (0.07, 95% CI = -0.11 to 0.22) or low (0.21, 95% CI = -0.08 to 0.50) carbohydrate content compared with CHO. Glycogen synthesis rates are enhanced when CHO-PRO are coingested after exercise compared with CHO only when the added energy of protein is consumed in addition to, not in place of, carbohydrate.

Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation.

The present study compared the effects of postexercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO)-only supplementation on muscle glycogen metabolism, anabolic cell signaling, and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol/kg dry wt) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after, despite substantial glycogen synthesis (176.1 ± 19.1 and 204.6 ± 27.0 mmol/kg dry wt in CHO and CHO+PROT, respectively; P = 0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5-h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared with CHO supplementation (54.6 ± 11.0 vs. 46.1 ± 9.8 min; P = 0.009). After the performance test, muscle glycogen was equally reduced in CHO+PROT and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389, or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6 ± 7.6 mg nitrogen/kg; P = 0.04) and higher than CHO (-10.7 ± 6.3 mg nitrogen/kg; P = 0.009). CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.NEW & NOTEWORTHY Endurance athletes competing consecutive days need optimal dietary intake during the recovery period. We report that coingestion of protein and carbohydrate soon after exhaustive exercise, compared with carbohydrate only, resulted in better performance the following day. The better performance after coingestion of protein and carbohydrate was not associated with a higher rate of glycogen synthesis or activation of anabolic signaling compared with carbohydrate only. Importantly, nitrogen balance was positive after coingestion of protein and carbohydrate, which was not the case after intake of carbohydrate only, suggesting that protein synthesis contributes to the better performance the following day.

Co-ingestion of carbohydrate and whey protein induces muscle strength and myofibrillar protein accretion without a requirement of satellite cell activation.

Muscle development is controlled by the balance between muscle protein synthesis and protein degradation. Protein supplementation has been widely known to enhance muscle protein synthesis, and carbohydrate supplementation may attenuate protein degradation. The purpose of this study was to compare the effects of whey protein plus carbohydrate (CP), whey protein (WP), and placebo (PLA) supplements on resistance training adaptations. Two-month old rats were trained by ladder climbing every 3 days for 8 weeks. PLA, WP, or CP was given immediately after each exercise session. Non-exercise rats were used as a sedentary control (SED). Total body composition was assessed and blood samples were collected before, middle, and end of training. The flexor hallucis longus (FHL) was excised 24 h after the last exercise session. Following training, maximal carrying capacity was significantly greater in CP than PLA and WP. This improved training performance in CP paralleled an increase in total muscle and myofibrillar protein content. Muscle and fiber cross sectional areas (CSA) were significantly increased by exercise training, with a concomitant increase in myonuclear domain. CP significantly elevated IGF-1 protein expression over SED, but there were no significant differences in myostatin, Pax7, MyoD, or myogenin across treatment groups. There was also no difference in the number of total nuclei in each fiber CSA among groups. Corticosterone levels were significantly elevated in PLA and WP over 8 weeks of training, whereas this change in corticosterone over time was not observed in the CP group. The results suggest that the greater improvement of maximal caring capacity for CP compared with PLA and WP was associated with a greater increase in myofibrillar protein content. Satellite cell activation did not appear to contribute to the observed gains in muscle hypertrophy and strength.

Effect of acute and short-term dietary fat ingestion on postprandial skeletal muscle protein synthesis rates in middle-aged, overweight, and obese men.

Muscle anabolic resistance to dietary protein is associated with obesity and insulin resistance. However, the contribution of excess consumption of fat to anabolic resistance is not well studied. The aim of these studies was to test the hypothesis that acute and short-term dietary fat overload will impair the skeletal muscle protein synthetic response to dietary protein ingestion. Eight overweight/obese men [46.4 ± 1.4 yr, body mass index (BMI) 32.3 ± 5.4 kg/m2] participated in the acute feeding study, which consisted of two randomized crossover trials. On each occasion, subjects ingested an oral meal (with and without fat emulsion), 4 h before the coingestion of milk protein, intrinsically labeled with [1-13C]phenylalanine, and dextrose. Nine overweight/obese men (44.0 ± 1.7 yr, BMI 30.1 ± 1.1 kg/m2) participated in the chronic study, which consisted of a baseline, 1-wk isocaloric diet, followed by a 2-wk high-fat diet (+25% energy excess). Acutely, incorporation of dietary amino acids into the skeletal muscle was twofold higher (P < 0.05) in the lipid trial compared with control. There was no effect of prior lipid ingestion on indices of insulin sensitivity (muscle glucose uptake, pyruvate dehydrogenase complex activity, and Akt phosphorylation) in response to the protein/dextrose drink. Fat overfeeding had no effect on muscle protein synthesis or glucose disposal in response to whey protein ingestion, despite increased muscle diacylglycerol C16:0 (P = 0.06) and ceramide C16:0 (P < 0.01) levels. Neither acute nor short-term dietary fat overload has a detrimental effect on the skeletal muscle protein synthetic response to dietary protein ingestion in overweight/obese men, suggesting that dietary-induced accumulation of intramuscular lipids per se is not associated with anabolic resistance.

Myofibrillar and Mitochondrial Protein Synthesis Rates Do Not Differ in Young Men Following the Ingestion of Carbohydrate with Milk Protein, Whey, or Micellar Casein after Concurrent Resistance- and Endurance-Type Exercise

ABSTRACTBackgroundWhey and micellar casein are high-quality dairy proteins that can stimulate postprandial muscle protein synthesis rates. How whey and casein compare with milk protein in their capacity to stimulate postprandial myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates during postexercise recovery is currently unknown.ObjectiveThe objective of this study was to compare postprandial MyoPS and MitoPS rates after protein-carbohydrate co-ingestion with milk protein, whey, or micellar casein during recovery from a single bout of concurrent resistance- and endurance-type exercise in young healthy men.MethodsIn a randomized, double-blind, parallel-group design, 48 healthy, young, recreationally active men (mean ± SEM age: 23 ± 0.3 y) received a primed continuous infusion of L-[ring-13C6]-phenylalanine and L-[ring-3,5-2H2]-tyrosine and ingested 45 g carbohydrate with 0 g protein (CHO), 20 g milk protein (MILK), 20 g whey protein (WHEY), or 20 g micellar casein protein (CASEIN) after a sequential bout of resistance- and endurance-type exercise (i.e., concurrent exercise). Blood and muscle biopsies were collected over 360 min during recovery from exercise to assess MyoPS and MitoPS rates and signaling through mammalian target of rapamycin complex 1 (mTORC1).ResultsDespite temporal differences in postprandial plasma leucine concentrations between treatments (P < 0.001), MyoPS rates over 360 min of recovery did not differ between treatments (CHO: 0.049% ± 0.003%/h; MILK: 0.059% ± 0.003%/h; WHEY: 0.054% ± 0.002%/h; CASEIN: 0.059% ± 0.005%/h; P = 0.11). When MILK, WHEY, and CASEIN were pooled into a single group (PROTEIN), protein co-ingestion resulted in greater MyoPS rates compared with CHO (PROTEIN: 0.057% ± 0.002%/h; CHO: 0.049% ± 0.003%/h; P = 0.04). MitoPS rates and signaling through the mTORC1 pathway were similar between treatments.ConclusionMyoPS and MitoPS rates do not differ after co-ingestion of either milk protein, whey protein, or micellar casein protein with carbohydrate during recovery from a single bout of concurrent resistance- and endurance-type exercise in recreationally active young men. Co-ingestion of protein with carbohydrate results in greater MyoPS, but not MitoPS rates, when compared with the ingestion of carbohydrate only during recovery from concurrent exercise. This trial was registered at Nederlands Trial Register: NTR5098.

Protein intake in the early recovery period after exhaustive exercise improves performance the following day.

The aim of the present study was to investigate the effect of protein and carbohydrate ingestion during early recovery from exhaustive exercise on performance after 18-h recovery. Eight elite cyclists (V̇o2max: 74.0 ± 1.6 ml·kg-1·min-1) completed two exercise and diet interventions in a double-blinded, randomized, crossover design. Participants cycled first at 73% of V̇o2max (W73%) followed by 1-min intervals at 90% of V̇o2max until exhaustion. During the first 2 h of recovery, participants ingested either 1.2 g carbohydrate·kg-1·h-1 (CHO) or 0.8 g carbohydrate + 0.4 g protein·kg-1·h-1 (CHO + PROT). The diet during the remaining recovery period was similar for both interventions and adjusted to body weight. After an 18-h recovery, cycling performance was assessed with a 10-s sprint test, 30 min of cycling at W73%, and a cycling time trial (TT). The TT was 8.5% faster (41:53 ± 1:51 vs. 45:26 ± 1:32 min; P < 0.03) after CHO + PROT compared with CHO. Mean power output during the sprints was 3.7% higher in CHO + PROT compared with CHO (1,063 ± 54 vs. 1,026 ± 53 W; P = 0.01). Nitrogen balance in the recovery period was negative in CHO and neutral in CHO + PROT (-82.4 ± 11.5 vs. 7.0 ± 15.4 mg/kg; P < 0.01). In conclusion, TT and sprint performances were improved 18 h after exhaustive cycling by CHO + PROT supplementation during the first 2 h of recovery compared with isoenergetic CHO supplementation. Our results indicate that intake of carbohydrate plus protein after exhaustive endurance exercise more rapidly converts the body from a catabolic to an anabolic state than carbohydrate alone, thus speeding recovery and improving subsequent cycling performance.NEW & NOTEWORTHY Prolonged high intensity endurance exercise depends on glycogen utilization and high oxidative capacity. Still, exhaustion develops and effective recovery strategies are required to compete in multiday stage races. We show that coingestion of protein and carbohydrate during the first 2 h of recovery is superior to isoenergetic intake of carbohydrate to stimulate recovery, and improves both endurance time-trial and 10-s sprint performance the following day in elite cyclists.

Effects of acute ingestion of whey protein with or without prior aerobic exercise on postprandial glycemia in type 2 diabetics.

Acute protein co-ingestion or a single bout of aerobic exercise can attenuate postprandial glycemia, but their combined effect has not been investigated in type 2 diabetics. Using a randomised crossover design, male type 2 diabetics (n = 8) [mean (95% CI); age, 55.0 (45.2, 64.8) year; BMI, 33.7 (25.6, 41.8) kg·m- 2; 2h glucose 14.0 (12.5, 15.5) mM] completed (1) 75g oral glucose tolerance test (OGTT) (CON); (2) OGTT supplemented with 0.33 g·kg BM- 1 of whey protein concentrate (PRO); or OGTT supplemented with PRO but preceded by a bout of aerobic cycling exercise (PRO + EX). Postprandial venous blood samples were collected for glucose, insulin, C-peptide and glucagon. Despite a fold-increase of 1.90 (1.26, 2.56; p < 0.05) in postprandial insulin compared to CON, PRO failed to attenuate postprandial glycemia measured by 2h glucose area under the curve. During PRO + EX, plasma glucose was elevated by 1.51 (0.5, 2.5) mM and 1.3 (0.3, 2.3) mM at 15 and 30min, respectively, compared to CON, but was lower by 1.60 (0.6, 2.6) mM and 1.5 (0.5, 2.5) mM at 90 and 120min, respectively (all p < 0.01). The additive effect of exercise and protein ingestion resulted in a fold-increase of 1.67 (1.35, 2.00; p < 0.05) in postprandial glucagon compared to CON. In type 2 diabetics, prior aerobic exercise altered the humoral response to co-ingestion of whey protein with a carbohydrate load, but neither protein ingestion alone nor when preceded by prior exercise attenuated postprandial glycemia.

The Effects of Protein Type and Added Leucine on Myofibrillar Protein Synthesis Following Concurrent Exercise

Protein ingestion increases skeletal muscle protein synthesis (MPS) rates. The effect of protein on MPS rates is enhanced by prior exercise. However, the effect of different types of protein on MPS is unclear and has not been evaluated following concurrent exercise. PURPOSE: The objective of this study was to determine the capacity of total milk protein (MILK), whey (WHEY), micellar casein (CASEIN), and soy protein, without (SOY) and with additional leucine (SOY+LEU) to support postprandial myofibrillar protein synthesis (MyoPS) following concurrent exercise. METHODS: 72 young recreationally active males participated in this parallel group, double-blind, randomized controlled trial consisting of two arms. In arm #1, 48 subjects (23±0.3 y) ingested 45 g of carbohydrate with either 0 g protein (CHO), or 20 g MILK, WHEY, or CASEIN protein. In arm #2, 36 subjects (23±0.5 y) ingested 45 g of carbohydrate with 20 g WHEY, SOY, or SOY+LEU. A primed continuous infusion of L-[ring-13C ]-phenylalanine with blood and muscle biopsies was applied to evaluate postprandial MyoPS over 360 minutes after exercise. RESULTS: In arm #1, MyoPS did not differ between treatments (P=0.12) during the postprandial period after exercise (FSR: CHO=0.051±0.003; MILK=0.061±0.003; WHEY=0.056±0.002; CASEIN=0.062±0.005 %/h). When MILK, WHEY, and CASEIN were collapsed into a single group (PROTEIN), protein-carbohydrate co-ingestion resulted in greater MyoPS rates than carbohydrate alone (FSR: PROTEIN=0.060±0.002; CHO=0.051±0.003 %/h; P=0.042). In arm #2 MyoPS did not differ between treatments (P=0.85) after exercise (FSR: WHEY=0.056±0.002; SOY=0.056±0.004; SOY+LEU=0.058±0.004 %/h). CONCLUSION: Total milk protein, whey, and micellar casein (arm #1) do not differ in their capacity to support postprandial MyoPS rates when co-ingested with carbohydrate following concurrent exercise in young males. Similarly, whey and soy protein, irrespective of leucine enrichment of soy (arm #2), do not differ in their capacity to support postprandial MyoPS after concurrent exercise. Co-ingestion of protein with carbohydrate results in greater postprandial MyoPS rates than carbohydrate alone, and may therefore represent a nutritional strategy to support skeletal muscle remodeling following concurrent exercise.

Co-ingestion of protein or a protein hydrolysate with carbohydrate enhances anabolic signaling, but not glycogen resynthesis, following recovery from prolonged aerobic exercise in trained cyclists.

The effect of carbohydrate (CHO), or CHO supplemented with either sodium caseinate protein (CHO-C) or a sodium caseinate protein hydrolysate (CHO-H) on the recovery of skeletal muscle glycogen and anabolic signaling following prolonged aerobic exercise was determined in trained male cyclists [n = 11, mean ± SEM age 28.8 ± 2.3years; body mass (BM) 75.0 ± 2.3kg; VO2peak 61.3 ± 1.6mlkg-1min-1]. On three separate occasions, participants cycled for 2h at ~ 70% VO2peak followed by a 4-h recovery period. Isoenergetic drinks were consumed at + 0 and + 2h of recovery containing either (1) CHO (1.2gkg-1 BM), (2) CHO-C, or (3) CHO-H (1.04 and 0.16gkg-1 BM, respectively) in a randomized, double-blind, cross-over design. Muscle biopsies from the vastus lateralis were taken prior to commencement of each trial, and at + 0 and + 4h of recovery for determination of skeletal muscle glycogen, and intracellular signaling associated with protein synthesis. Despite an augmented insulin response following CHO-H ingestion, there was no significant difference in skeletal muscle glycogen resynthesis following recovery between trials. CHO-C and CHO-H co-ingestion significantly increased phospho-mTOR Ser2448 and 4EBP1 Thr37/46 versus CHO, with CHO-H displaying the greatest change in phospho-4EBP1 Thr37/46. Protein co-ingestion, compared to CHO alone, during recovery did not augment glycogen resynthesis. Supplementing CHO with intact sodium caseinate or an insulinotropic hydrolysate derivative augmented intracellular signaling associated with skeletal muscle protein synthesis following prolonged aerobic exercise.

Effect of carbohydrate and protein solutions consumed during a moderate-intensity exercise on post-exercise appetite

This study aimed to investigate whether co-ingestion of carbohydrate and protein during exercise affect the post-exercise ad libitum food intake. Twelve healthy active male participants (mean±SD, age: 20±1; height: 176±6cm; weight: 63.6±5.3kg; VO2peak: 51.2±7.1ml/kg/min) completed three main experimental trials in a randomized cross-over design. In each trial, the participants completed 1h of ergometer cycling at 60% VO2peak, followed by 2h recovery. The participants were required to consume one of three solutions every 15min during exercise: distilled water (DW), carbohydrate-electrolyte solution (CE), and carbohydrate-electrolyte-protein solution (CEP). The energy was matched between the two latter solutions. The CHO-to-protein ratio in CEP was 2:1. At the end of recovery period, participants were provided with pizza lunch ad libitum, and the amount consumed was recorded. Several subjective feelings, appetite scores and blood glucose were determined during the experimental trials. No differences were found in either the amount of consumed pizza (DW vs. CE vs. CEP: 607±128 vs. 592±119 vs. 599±125g, P=0.845) or the appetite score before pizza was consumed (DW vs. CE vs. CEP: 14±9 vs. 12±14 vs. 14±10, P=0.357) among the three trials. The blood glucose concentrations during exercise were higher in the CE and CEP trials than in the DW trial. In conclusion, different solutions consumed during a 1h moderate-intensity exercise in the present study did not affect post-exercise appetite.

Co-ingestion of carbohydrate and whey protein increases fasted rates of muscle protein synthesis immediately after resistance exercise in rats.

The objective of the study was to investigate whether co-ingestion of carbohydrate and protein as compared with protein alone augments muscle protein synthesis (MPS) during early exercise recovery. Two months old rats performed 10 repetitions of ladder climbing with 75% of body weight attached to their tails. Placebo (PLA), whey protein (WP), or whey protein plus carbohydrate (CP) was then given to rats by gavage. An additional group of sedentary rats (SED) was used as controls. Blood samples were collected immediately and at either 1 or 2 h after exercise. The flexor hallucis longus muscle was excised at 1 or 2 h post exercise for analysis of MPS and related signaling proteins. MPS was significantly increased by CP compared with PLA (p<0.05), and approached significance compared with WP at 1 h post exercise (p = 0.08). CP yielded a greater phosphorylation of mTOR compared with SED and PLA at 1 h post exercise and SED and WP at 2 h post exercise. CP also increased phosphorylation of p70S6K compared with SED at 1 and 2 h post exercise. 4E-BP1 phosphorylation was inhibited by PLA at 1 h but elevated by WP and CP at 2 h post exercise relative to SED. The phosphorylation of AMPK was elevated by exercise at 1 h post exercise, and this elevated level was sustained only in the WP group at 2 h. The phosphorylation of Akt, GSK3, and eIF2Bε were unchanged by treatments. Plasma insulin was transiently increased by CP at 1 h post exercise. In conclusion, post-exercise CP supplementation increases MPS post exercise relative to PLA and possibly WP, which may have been mediated by greater activation of the mTOR signaling pathway.

The effect of carbohydrate and protein co-ingestion on energy substrate metabolism, sense of effort, and affective responses during prolonged strenuous endurance exercise

This study examined the effect of carbohydrate (CHO) and protein (PRO) co-ingestion on energy substrate metabolism, sense of effort, and affective responses during prolonged strenuous endurance exercise. In a four-stage randomized cross-over design, 10 male endurance runners (age: 27.3±1.4yrs; height: 172.9±1.1cm, weight: 63.5±1.6kg; body fat: 9.0±1.4%; V̇O2max: 62.9±1.8ml/kg/min) ran on a treadmill at 70% of their individual V̇O2max for 90min. There were two CHO and PRO treatments (CA: CHO+alpha-lactalbumin and CW: CHO+whey PRO isolate), one CHO treatment (CC: CHO+CHO), and a placebo control (CON). On each occasion, subjects consumed 5ml/kg according to their body weight (kg) immediately before and 2ml/kg every 15min during exercise. Blood samples were collected at 0min, 30min, 60min and 90min of exercise to measure glucose, lactate, insulin, and cortisol levels. The extent of physical sensation (abdominal discomfort, leg muscle pain), the sense of effort (rating of perceive exertion, RPE), and affective responses (pleasure-displeasure, arousal) were evaluated by numeric scales before, during, and immediately after exercise. Blood glucose and insulin concentrations in the CA, CW, and CC treatments were higher than in the CON at 90min (P<0.05). Muscle pain (evaluated by a single item, 0 to 10 pain intensity scale from “no pain at all” to “extremely unbearable”) was lower following CA ingestion than CON and CW ingestion, at 75min (vs. CON and CW, 1.95±0.61 vs. 3.70±1.00 and 3.60±1.02, P<0.05) and 90min (vs. CON & CW: 2.40±0.76 vs. 4.20±0.99 and 4.05±1.1, P<0.05). RPE (evaluated by a 15-point, 6 to 20 rating scale ranging from “very, very light” to “very, very hard”) following treatment with CA was lower than with CON and CW at 90min (vs. CON and CW: 11.30±1.14 vs. 14.20±1.30 and 13.30±1.24, P<0.05). Compared with CON and CW, CA enhanced the feeling of pleasure (evaluated by feeling scale from −5, “very bad” to +5, “very good”) at 90min (vs. CON and CW, 2.20±0.67 vs. −0.30±0.94 and 0.20±0.91, P<0.05). Co-ingestion of CHO and alpha-lactalbumin protein attenuates muscle pain, and therefore alleviates the sense of effort and enhances affective responses during 90min of strenuous running at 70% V̇O2max.

Postexercise muscle glycogen resynthesis in humans.

Since the pioneering studies conducted in the 1960s in which glycogen status was investigated using the muscle biopsy technique, sports scientists have developed a sophisticated appreciation of the role of glycogen in cellular adaptation and exercise performance, as well as sites of storage of this important metabolic fuel. While sports nutrition guidelines have evolved during the past decade to incorporate sport-specific and periodized manipulation of carbohydrate (CHO) availability, athletes attempt to maximize muscle glycogen synthesis between important workouts or competitive events so that fuel stores closely match the demands of the prescribed exercise. Therefore, it is important to understand the factors that enhance or impair this biphasic process. In the early postexercise period (0-4 h), glycogen depletion provides a strong drive for its own resynthesis, with the provision of CHO (~1 g/kg body mass) optimizing this process. During the later phase of recovery (4-24 h), CHO intake should meet the anticipated fuel needs of the training/competition, with the type, form, and pattern of intake being less important than total intake. Dietary strategies that can enhance glycogen synthesis from suboptimal amounts of CHO or energy intake are of practical interest to many athletes; in this scenario, the coingestion of protein with CHO can assist glycogen storage. Future research should identify other factors that enhance the rate of synthesis of glycogen storage in a limited time frame, improve glycogen storage from a limited CHO intake, or increase muscle glycogen supercompensation.

Effects Of A Nutritive Administration Of Carbohydrates And Protein By Food On Skeletal Muscle Inflammation And Damage After Acute Endurance Exercise

: Scientific Background: Protein ingestion has been recently described to enhance the effects of carbohydrate drinks on skeletal muscle recovery. The aim of this study was to investigate the beneficial effects of a co-ingestion of carbohydrate and protein by food in amateur sportsmen. METHODS: 16 male subjects performed a 10km run at 80% of individual ANS. Immediately after exercise subjects ingest either nothing (control), carbohydrates by eating white bread, or a combination of carbohydrates and protein by eating white bread and a sour milk cheese. Blood samples were taken at different time points. Serum glucose, serum insulin, serum creatine kinase (CK) and the serum levels of IL6, IL 10, MIF and TNF alpha were determined. RESULTS: Blood glucose was increased by endurance exercise. Blood glucose levels decreased rapidly after exercise, here the strongest decrease could be observed in the bread/cheese group. Food uptake results in strong increase of serum insulin not differing between bread and bread/cheese. CK serum levels as a marker for skeletal muscle damage strongly increased 24h after exercise in the control and bread group but not in the bread cheese group. Exercise resulted in an increase of serum IL 6 and MIF which was reduced in the bread/cheese group. Serum IL 10 was increased by bread/cheese ingestion. TNF alpha serum concentration was decreased by exercise. This decrease was antagonized by bread/cheese ingestion. CONCLUSION: Our data demonstrate that uptake of a combination of protein and carbohydrate by food immediately after endurance exercise effect’s physiological responses like blood glucose levels of the individual’s and antagonize the induction of serum CK, a marker of skeletal muscle damage. Moreover this nutritive carbohydrate/protein uptake seems to influence the inflammatory response of the skeletal muscle indicated by reduced concentrations of proinflammatory markers and an increase of anti-inflammatory markers.

Co-ingestion of carbohydrate and pea protein does not enhance muscle recovery after strenuous exercise

OBJECTIVE: To assess the influence of carbohydrate and added pea protein concentrate supplementation on muscle recovery after a military operation called Leader's Reaction Test. METHODS: Twenty-four soldiers from the Brazilian Army were divided into three equal groups (n=8). They received either carbohydrate (0.8.g/kg body weight/h), carbohydrate+carbohydrate (1.0.g/kg body weight/h), or carbohydrate+protein (0.8 g/kg body weight/h of carbohydrate + 0.2 g/kg body weight/h of protein), immediately, 60, and 120 minutes after the Leader’s Reaction Test. Prior, immediately after and 24 hours after the Leader’s Reaction Test, maximal isometric strength and body composition were assessed. Blood samples were also collected for later analysis of concentrations of lactate dehydrogenase and creatine kinase. RESULTS: Twenty-four hours after the Leader's Reaction Test, maximal creatine kinase levels were significantly lower than its levels immediately after (501.00 ± 422.09 versus 275.29 ± 242.08 U/L (carbohydrate); 616.88 ± 291.45 versus 334.57 ± 191.61 U/L (carbohydrate+carbohydrate); and 636.75 ± 340.67 versus 382.88 ± 234.42 U/L (carbohydrate+protein), p=0.004). The maximal isometric strength and lactate dehydrogenase levels were not significantly different during the time trials. CONCLUSION: The present findings suggest that carbohydrate+protein co-ingestion did not improve the recovery of muscle function nor did it attenuate post-exercise muscle damage markers over carbohydrate alone.

Effects of consumption of main and side dishes with white rice on postprandial glucose, insulin, glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 responses in healthy Japanese men

The co-ingestion of protein, fat and fibre with carbohydrate reportedly affects postprandial glucose, insulin and incretin (glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1)) responses. However, the effects of combination dishes with carbohydrate-rich foods at typically eaten amounts remain unclear. The objective of the present study was to evaluate the effects of consuming recommended amounts of side dishes with boiled white rice in the same meal on postprandial plasma glucose, insulin and incretin hormone responses. A total of nine healthy male volunteers consumed four different meals in a random order on separate days. The test meals were as follows: S, white rice; SM, addition of protein-rich main dishes to the S meal; SMF, addition of a fat-rich food item to the SM meal; SMFV, addition of vegetables to the SMF meal. Plasma glucose, GIP and GLP-1 and serum insulin concentrations were determined during a 3h period after consumption of these meals. Postprandial glucose responses were lower after SMFV meal consumption than after consumption of the other meals. The incremental AUC for GIP (0-180min) were largest after consumption of the SMF and SMFV meals, followed by that after SM meal consumption, and was smallest after S meal consumption (P<0·05). Furthermore, we found GIP concentrations to be dose dependently increased by the fat content of meals of ordinary size, despite the amount of additional fat being small. In conclusion, the combination of recommended amounts of main and vegetable side dishes with boiled white rice is beneficial for lowering postprandial glucose concentrations, with an increased incretin response, when compared with white rice alone.

Coingestion of carbohydrate and protein during training reduces training stress and enhances subsequent exercise performance

Researchers have focused primarily on investigating the effects of coingesting carbohydrate (CHO) and protein (PRO) during recovery and, as such, there is limited research investigating the benefits of CHO+PRO coingestion during exercise for enhancing subsequent exercise performance. The aim of this study was to investigate whether coingestion of CHO+PRO during endurance training would enhance recovery and subsequent exercise performance. Ten well-trained male cyclists (aged 29.7 ± 7.5 years; maximal oxygen uptake, 66.2 ± 6 mL·kg(-1)·min(-1)) took part in a randomized, double-blind, cross-over trial. Each trial consisted of a 2.5-h morning training bout during which the cyclists ingested a CHO+PRO or energy-matched CHO beverage followed by a 4-h recovery period and a subsequent performance time trial (total work, 7 kJ·kg(-1)). Blood was collected before and after exercise. Time-trial performance was 1.8% faster in the CHO+PRO trial compared with the CHO trial (p = 0.149; 95% CI, -13 to 87 s; 75.8% likelihood of benefit). The increase in myoglobin level from before the training bout to after the training bout was lower in the CHO+PRO trial (0.74 nmol·L(-1); 95% CI, 0.3-1.17 nmol·L(-1)) compared with the CHO trial (1.16 nmol·L(-1); 95% CI, 0.6-1.71 nmol·L(-1)) (p = 0.018). Additionally, the decrease in neutrophil count over the recovery period was greater in the CHO+PRO trial (p = 0.034), and heart rate (p < 0.022) and rating of perceived exertion (RPE) (p < 0.01) were lower during training in the CHO+PRO trial compared with the CHO trial. Ingesting PRO, in addition to CHO, during strenuous training lowered exercise stress, as indicated by reduced heart rate, RPE, and muscle damage, when compared with CHO alone. CHO+PRO ingestion during training is also likely to enhance recovery, providing a worthwhile improvement in subsequent cycling time-trial performance.