Clove Oil Nanoemulsion Research Articles

Effect of clove and bamboo essential oil nanoemulsion in extending the shelf life of meat ball

Effect of clove and bamboo essential oil nanoemulsion in extending the shelf life of meat ball

Design of Delivery Systems (Nanoemulsions and Biopolymer Nanoparticles) of Cloves Essential Oil: Preparation, Characterizations, Study the Release, and Antioxidant Activity

Abstract BACKGROUND: Clove oil has a wide range of therapeutic and pharmaceutical applications. It is also used in the manufacture of pesticides and antifungals, as well as many advantages. The study aims to improve the properties of essential oils, especially clove oil, and make them more stable by the design of delivery systems (nanoemulsions and biopolymer nanoparticles). METHODS: Clove oil was mixed with water in the presence of tween-20 as a polysorbate-type nonionic surfactant, and then carboxymethyl cellulose was added with different ratios of glycerin as a cross-linking agent. The formation of the clove nanogel was tested by spectrophotometric assay 2.2.2 Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), dynamic light scattering (DLS), and morphology method field emission scanning electron microscopy (FESEM), also the rate of clove oil release during different periods, and hemolytic and antioxidant activity were tested. RESULTS: The nanoparticles were demonstrated by DLS and FESEM. The polydispersity index value was 0.279, indicating good monodispersity and good stability, while higher release values were obtained when glycerol was not present as a cross-linker. In addition to the higher antioxidant, values of the clove oil nano emulsion (CNE) have a maximum inhibition of 65% at a concentration of 100 μg/mL, whereas clove nano-gel (CNG) at the same concentration exhibited 59.2% inhibition. The calculated IC50 values of CNG and CNE were 6.58 and 3.25 μg/mL, respectively. CONCLUSION: The study proved through the results obtained that nanocomposites help stabilize components, especially volatile oil, which increases their effectiveness.

Modulation efficiency of clove oil nano-emulsion against genotoxic, oxidative stress, and histological injuries induced via titanium dioxide nanoparticles in mice

Titanium dioxide nanoparticles (TiO2-NPs) have found wide applications in medical and industrial fields. However, the toxic effect of various tissues is still under study. In this study, we evaluated the toxic effect of TiO2-NP on stomach, liver, and kidney tissues and the amelioration effect of clove oil nanoemulsion (CLV-NE) against DNA damage, oxidative stress, pathological changes, and the apoptotic effect of TiO2-NPs. Four groups of male mice were subjected to oral treatment for five consecutive days including, the control group, the group treated with TiO2-NPs (50 mg/kg), the group treated with (CLV-NE) (5% of the MTD), and the group treated with TiO2-NPs plus CLV-NE. The results revealed that the treatment with TiO2-NPs significantly caused DNA damage in the liver, stomach, and kidney tissues due to increased ROS as indicated by the reduction of the antioxidant activity of SOD and Gpx and increased MDA level. Further, abnormal histological signs and apoptotic effect confirmed by the significant elevation of p53 expression were reported after TiO2-NPs administration. The present data reported a significant improvement in the previous parameters after treatment with CLV-NE. These results showed the collaborative effect of the oils and the extra role of nanoemulsion in enhancing antioxidant effectiveness that enhances its disperse-ability and further promotes its controlled release. One could conclude that CLV-NE is safe and can be used as a powerful antioxidative agent to assess the toxic effects of the acute use of TiO2-NPs.

Optimization of Clove Oil Nanoemulsions: Evaluation of Antioxidant, Antimicrobial, and Anticancer Properties

Clove essential oil is traditionally used as an anesthetic, analgesic, or insecticide, and recently, its applications as an antimicrobial, antioxidant, or anticancer agent have been explored. Nanoemulsions are thermodynamically unstable dispersions (d < 100 nm) produced by mixing two immiscible phases, which, in many cases, improve the stability and biological activities of functional ingredients for pharmaceutical, cosmetic, or food applications. This research optimized the formation of clove essential oil nanoemulsions by employing response surface methodology. The surfactant concentration was minimized by modifying the percentage of clove oil (0–100%), surfactant content (1–4%), and oil phase content (0–20%). In the optimum conditions, a nanoemulsion (93.19 ± 3.92 nm) was produced using 1.0% surfactant and 2.5% oil phase of which 50.7% was clove essential oil. The optimized nanoemulsion was stable in rapid stability tests (centrifugation, freezing–thawing, and heating–cooling), but its average droplet size increased during storage at different temperatures. The nanoemulsion contains a phenolic content equivalent to 736 mg gallic acid/mL. However, the antioxidant capacity of the essential oil (IC50 = 0.78 µg/mL) was dismissed in the nanoemulsion (IC50 = 2.43 µg/mL). The antimicrobial activity of the nanoemulsion showed strain–dependent behavior with MIC ranging from 0.0468 to 0.75 mg/mL, where E. coli and S. typhimurium were the most susceptible pathogenic bacteria. Finally, nanoencapsulation of clove oil showed higher in vitro cytotoxic activity against Caco–2 cancer cells (227 μg/mL) than free clove essential oil (283 μg/mL), but nanoemulsion (306 μg/mL) was less effective than oil (231 μg/mL) in the HT–29 line. This research shows the potential of clove essential oil nanoemulsions for developing biological therapies to treat diseases.

Antimicrobial, Antibiofilm, and Anticancer Activities of Syzygium aromaticum Essential Oil Nanoemulsion.

In the current study, clove oil nanoemulsion (CL-nanoemulsion) and emulsion (CL-emulsion) were prepared through an ecofriendly method. The prepared CL-nanoemulsion and CL-emulsion were characterized using dynamic light scattering (DLS) and a transmission electron microscope (TEM), where results illustrated that CL-nanoemulsion droplets were approximately 32.67 nm in size and spherical in shape, while CL-nanoemulsion droplets were approximately 225.8 nm with a spherical shape. The antibacterial activity of CL-nanoemulsion and CL-emulsion was carried out using a microbroth dilution method. Results revealed that the preferred CL-nanoemulsion had minimal MIC values between 0.31 and 5 mg/mL. The antibiofilm efficacy of CL-nanoemulsion against S. aureus significantly decreased the development of biofilm compared with CL-emulsion. Furthermore, results illustrated that CL-nanoemulsion showed antifungal activity significantly higher than CL-emulsion. Moreover, the prepared CL-nanoemulsion exhibited outstanding antifungal efficiency toward Candida albicans, Cryptococcus neoformans, Aspergillus brasiliensis, A. flavus, and A. fumigatus where MICs were 12.5, 3.12, 0.78, 1.56, and 1.56 mg/mL, respectively. Additionally, the prepared CL-nanoemulsion was analyzed for its antineoplastic effects through a modified MTT assay for evaluating apoptotic and cytotoxic effects using HepG2 and MCF-7 cell lines. MCF-7 breast cancer cells showed the lowest IC50 values (3.4-fold) in CL-nanoemulsion relative to that of CL-emulsion. Thus, CL-nanoemulsion induces apoptosis in breast cancer cells by inducing caspase-8 and -9 activity and suppressing VEGFR-2. In conclusion, the prepared CL-nanoemulsion had antibacterial, antifungal, and antibiofilm as well as anticancer properties, which can be used in different biomedical applications after extensive studies in vivo.

Benchmark Dose Approach to DNA and Liver Damage by Chlorpyrifos and Imidacloprid in Male Rats: The Protective Effect of a Clove-Oil-Based Nanoemulsion Loaded with Pomegranate Peel Extract.

Pesticides are widely used around the world to increase crop production. They also have negative impacts on animals, humans, and the ecosystem. This is the first report evaluating a novel pomegranate-extract-loaded clove-oil-based nanoemulsion (PELCN) and its potential for reducing oxidative stress and DNA damage, as well as its hepatoprotective effects against imidacloprid (IM) and chlorpyrifos (CPF) toxicity in male rats. The benchmark dose (BMD) approach was also used to study the dose-response toxicity of IM and CPF. IM and CPF were administered daily for 28 days at doses of 14, 28, and 54 mg/kg body weight (bw) of IM and 1, 2, and 4 mg/kg bw of CPF via drinking water. The PELCN was administered orally at a dose of 50 mg/kg bw/day of pomegranate extract, 500 mg/kg bw of the clove oil nanoemulsion, and IM or CPF at high doses in the drinking water. In male rats, IM and CPF caused a reduction in body weight gain and hepatotoxic effects as evidenced by increases in the liver enzymes AST, ALT, and ALP. They caused oxidative damage in the liver of male rats as indicated by the decreased liver activity of the GST, GPX, SOD, and CAT enzymes and decreased serum TAC. IM and CPF produced a significant dose-dependent increase in DNA damage in hepatocyte cells, resulting in moderate to severe liver damage with cells that are more inflammatory and have enlarged sinusoids and compacted nuclei. IM had a higher BMD than CPF for both body and liver weight, suggesting that CPF was more dose-dependently toxic than IM. Albumin was a highly sensitive liver biomarker for IM, while total protein was a biomarker for the CPF-treated rats. GPx was an extremely sensitive biomarker of oxidative stress in the IM treatment, while CAT and GPx were highly sensitive parameters in the CPF-treated rats. Therefore, at comparable doses, CPF has a higher potential to cause liver damage and oxidative stress than IM. The hepatotoxicity of IM and CPF can be mitigated by administering a nanoemulsion containing clove oil and pomegranate extract. The nanoemulsion acts as a protector against the oxidative stress caused by these insecticides, especially at high doses. The nanoemulsion based on clove oil increases the bioavailability and stability of the pomegranate extract, which has antioxidant properties.

Nanoemulsions of Clove Oil Stabilized with Chitosan Oleate—Antioxidant and Wound-Healing Activity

Clove oil (CO) is a powerful antioxidant essential oil (EO) with anti-inflammatory, anesthetic, and anti-infective properties. It can be therefore considered a good candidate for wound-healing applications, especially for chronic or diabetic wounds or burns, where the balance of reactive oxygen species (ROS) production and detoxification is altered. However, EOs require suitable formulations to be efficiently administered in moist wound environments. Chitosan hydrophobically modified by an ionic interaction with oleic acid (chitosan oleate, CSO) was used in the present work to stabilize CO nanoemulsions (NEs). The dimensions of the NE were maintained at around 300 nm as the volume distribution for up to six months, and the CO content did not decrease to under 80% over 4 months, confirming the good stabilizing properties of CSO. The antioxidant properties of the CO NE were evaluated in vitro by a 2,2-diphenil-2-picrylhydrazyl hydrate (DPPH) assay, and in fibroblast cell lines by electron paramagnetic resonance (EPR) using α-phenyl-N-tert-butyl nitrone (PBN) as a spin trap; a protective effect was obtained comparable to that obtained with α-tocopherol treatment. In a murine burn model, the ability of CO formulations to favor macroscopic wound closure was evidenced, and a histological analysis revealed a positive effect of the CO NE on the reparation of the lesion after 18 days. Samples of wounds at 7 days were subjected to a histological analysis and parallel dosage of lipid peroxidation by means of a thiobarbituric acid-reactive substances (TBARS) assay, confirming the antioxidant and anti-inflammatory activity of the CO NE.

Development and evaluation of clove and cinnamon oil-based nanoemulsions against adult fleas (Xenopsyllacheopis)

Adult fleas is one of the important vector borne insects which transmitting many diseases to mammals (cats, dogs, rodents, and humans) and birds worldwide. Because of the impact of climatic changes, the distribution of transmitted vector-borne pathogens may change, as well as the host ranges and the spread of diseases. Rat flea, Xenopsylla cheopis, can transmit Plague, a dangerous infectious disease caused by the bacterium Yersinia pestis. As the widespread use of synthetic insecticides to control fleas for a long time led to the development of insect resistance, and adverse health effects on humans, animals, and the ecosystem. This is the first work for formulating and evaluating clove and cinnamon oil nanoemulsions and studying their effect on adult flea. Clove and cinnamon nanoformulations were prepared and subjected to physicochemical and stability studies. Results showed that the small droplet size of nanoformulations was 158.4 nm and 282.5 nm of clove and cinnamon oils at a ratio of 1:2 of oil: tween 80 with 15 min as the sonication time. Cinnamon nanoemulsions have high insecticidal activity against flea compared to clove nanoemulsions. The LC50 account for 14.50 μg/ml and 26.42 μg/ml with a 39.90% increase in cinnamon nanoemulsion activity compared to clove nanoemulsion, respectively. Cinnamon nanoformulation is solvent-free and did not have any toxic effects on experimental animals and EC50 more than 100 mg/l (EC50 > 100 mg/l) on Vibrio fischeri bacteria. Therefore, it considers an eco-friendly green nano-insecticide for controlling Xenopsylla cheopis flea.

Chemical composition of essential oils from leaf and bud of clove and their impact on the antifungal and mycotoxin inhibitory activities of clove oil-in-water nanoemulsions

The impacts of clove oil (CO) type on the physicochemical properties, antifungal activities, and mycotoxin inhibitory efficacies of CO-in-water nanoemulsions were investigated. By blending 25 wt% of clove oil with 75 wt% of corn oil in oil phase (5 wt%), clove bud oil (CBO)-in-water nanoemulsion and clove leaf oil (CLO)-in-water nanoemulsion showed highly physical stable with the mean particle size < 170 nm. The concentration of major chemical constituents such as eugenol and caryophyllene in nanoemulsion maintained constant during storage time. The antifungal activities of nanoemulsions against two Fusarium graminearum isolates were strongly dependent on the CO concentration. For instance, CLO nanoemulsion displayed an appreciable higher antifungal activity compared to CBO nanoemulsion at a lower CO concentration. Both nanoemulsions could effectively inhibit fungal mycelial growth and spore germination , but in a different antifungal mode of actions. Regarding the mycotoxin inhibitory efficacy, both CO nanoemulsions could effectively inhibit mycotoxin production including deoxynivalenol (DON) and its derivatives: 3-acetyl-deoxynivalenol (3ADON), 15-acetyl-deoxynivalenol (15ADON). • Chemical composition of clove bud oil (CBO) and clove leaf oil (CLO) showed differences. • The physicochemical stability of clove oil (CO)n nanoemulsions were fabricated. • Antifungal activity of CO nanoemulsions was in a dose-dependent manner. • Different antifungal modes of actions between CBO and CLO was observed. • Both CO nanoemulsions could effectively inhibit mycotoxin production.

Efficiency of Clove Oil Nanoemulsion in Modulating Titanium Dioxide-Induced Some Disorders in the lung of the Mice

Efficiency of Clove Oil Nanoemulsion in Modulating Titanium Dioxide-Induced Some Disorders in the lung of the Mice

Production of Clove Oil Nanoemulsion Using Whey Protein- Maltodextrin Conjugates and Chitosan

Clove oil has benefits for both food and pharmaceutical purposes. However its use is limited due to several properties such as susceptibility to oxidation, poor water solubility, react with other components, and toxic at high concentration. The limitation of its usage can be overcome through emulsion technology. Therefore, this study aims to obtain clove oil nanoemulsion through preparation using whey protein-maltodextrin conjugates. These conjugates act as an emulsifier and a first layer, meanwhile chitosan as a stabilizer and also a second layer. The formulation used different chitosan concentrations, at 0.2, 0.4, and 0.6%, then processed using High-Pressure Homogenizer (HPH) in 3 and 5 cycles. The result showed that the concentration of chitosan and the number of HPH cycles affected the clove oil emulsion performance. Furthermore, the formulation using 0.6% chitosan concentration and 5 cycles of HPH produced the best clove oil nanoemulsion in droplet size, polydispersity index, and zeta potential were 147.5, 0.214, and -52.4, respectively. The results of this study provide information on the potential of emulsion formulations using WPM conjugate and chitosan to produce clove oil nanoemulsions that can be utilized in food and pharmaceutical products.

Physico-chemical characterization of ultrasound assisted clove oil-loaded nanoemulsion: As enhanced antimicrobial potential

Clove oil has a high eugenol content, making it an effective antimicrobial essential oil; nevertheless, its low water solubility, high volatility, and organoleptic qualities limit its use in food systems. As a result, we created an antibacterial system using clove oil-in-water nanoemulsion. Clove oil nanoemulsions were produced using whey protein concentrate (0.1–1%) as an emulsifier by ultrasonication and various physico-chemical characteristics (stability, particle size, zeta-potential, and poly dispersity index) were investigated. Mean particle size, zeta potential and polydispersity index of the most stable nanoemulsion were 279.0 ± 8.43 nm, -34.5 ± 0.12 mV, and 0.179 ± 0.012, respectively. Most stable nanoemulsion was fairly stable at different processing parameters such as various pH (3.0 – 7.0), temperature ranges (63 – 121 °C), and ionic strengths (0.1 – 1.0 M NaCl). Finally, antimicrobial activities, such as minimum inhibitory concentration was found with 50 µL, whereas minimum bactericidal concentration was observed to be 90 µL after 8 h contact time, against E. coli and B. subtilis strains.

Physical, morphological and storage stability of clove oil nanoemulsion based delivery system.

Clove oil based Nanoemulsions (NE) were prepared ultrasonically using Tween 80 and soy lecithin as synthetic and natural surfactants, respectively. The developed NEs were characterized for various parameters (particle size, polydispersity index, zeta potential, morphology, viscosity, colour, turbidity and pH) and the comparative effect of both the surfactants at variable levels (oil:tween 80-1:1, 1:2, 1:3 and 1:4 and oil: soy lecithin- 1:1, 1:1.5 and 1:2) was assessed. It was found that the type of surfactant and oil to surfactant ratio significantly affected particle size and stability of NEs. The NE prepared using tween 80 @1:3 had smallest average droplet diameter (40.9 nm). The formulated NEs were stored at 25 °C and 4 °C and analyzed for turbidity, pH and phase separation up to 90 days. Results revealed that the type and concentration of the surfactant significantly influenced the particle size and stability of NEs. NEs prepared using tween 80 were found to be more viscous than those prepared with soy lecithin. The prepared clove oil NEs have important implication to be used as a natural delivery system to increase the shelf life of food products.

Development of Phosphatidylcholine/Tween 80 based biocompatible clove oil-in-water nanoemulsion as a green nanocarrier for controlled herbicide delivery

Recently, the development of ecofriendly and biocompatible agrochemical delivery systems has garnered widespread attention because of their great potential in sustainable agri-food applications. Atrazine (ATZ) is a globally used herbicide used to control weeds, but it suffers from poor aqueous solubility, poor efficacy, and environmental loss. Herein, we report a novel, eco-friendly and biocompatible clove oil-based nanoemulsion as a green nanocarrier to enhance the solubility, bioavailability, and control release of ATZ. Food grade surfactants, Tween 80 and Phosphatidylcholine (PC) were used to formulate clove oil nanoemulsion with size <200 nm using ultrasonic emulsification technique, without any use of organic solvent. The ATZ encapsulation efficiency in NEm was greater than 95%. DLS confirms the nanosize (106 nm) and monodispersity of NEm. HRTEM reveals the spherical morphology of the nanodroplets. FTIR and DSC confirm the successful incorporation of ATZ inside the NEm oil droplet core. ATZ loaded NEm showed excellent thermal and storage stability, low Ostwald ripening rate, slow and sustained herbicide release behavior, which is of vital importance for an herbicide formulation. The release rate was better than commercial ATZ and free ATZ formulations. Results from herbicidal activity assays demonstrate that ATZ NEm exhibited excellent herbicidal activity even at low concentrations as compared to commercial ATZ analogs. In consideration of biocompatible excipients, free of organic solvent, and a simple fabrication process, ATZ loaded clove oil NEm can hold great potential in weed control and sustainable agri-food applications.

Ultrasonication induced synthesis of TPGS stabilized clove oil nanoemulsions and their synergistic effect against breast cancer cells and harmful bacteria

The present work reports the fabrication of TPGS assisted clove oil in water nanoemulsion for the delivery of polyphenolic nutraceuticals i.e. curcumin and resveratrol and the analysis of their synergetic healing potential. The fabrication procedure was undertaken by the use of high energy ultrasonic cavitation technique. The morphological and rheological properties of the fabricated systems were analyzed and optimized using numerous techniques. The current study throws light on the influence of surfactant type and composition on the mixing stability of oil phase. Both curcumin and resveratrol were effectively loaded in the nanoemulsion, and their loading efficiencies were found to be 99.87 and 99.38%, respectively. The study was undertaken with the aim of exploring the versatile therapeutic potential by elevating the established antimicrobial activity of curcumin and resveratrol and testing their prospects against breast cancer cell lines. The results demonstrated the antimicrobial activity of nanoemulsion (without nutraceuticals) itself against gram-positive and gram-negative bacteria owing to the clove oil which would be an asset in accomplishing better results. In combination with curcumin and resveratrol, it exhibited enhanced effect against different bacterial strains thus showcasing an increase in its efficacy. Furthermore, the anti-tumor activity of prepared formulations has been tested on MDA MB −231 and Hep G2 breast cancer cell lines to broaden the horizons of biological applications of the formulated system.

Shelf life extension of muffins coated with cinnamon and clove oil nanoemulsions.

Clove and cinnamon based nanoemulsions (NE) were prepared with different levels of soy lecithin as a surfactant employing ultrasonication treatment. Increase in surfactant levels decreased the particle size (PS), polydispersity index (PDI) and zeta potential in both the NE. Cinnamon and clove based nanoemulsions were optimized at 1.5 and 2.0% SL, respectively based on their PS and PDI value. Nanoemulsions having the highest thermo stability were further selected to coat the muffin samples. NE Coated and uncoated muffins were evaluated for changes in density, moisture content, water activity, weight loss, texture, antioxidant activity, microbial activity and sensory scores during storage. NE coating significantly increased antioxidant activity of muffins by 26.89% (Cinnamon oil NE) and 37.31% (Clove oil NE). Furthermore during storage, coating of NE maintains the texture of the muffins, reduce the weight loss, density and moisture content to a greater extent in comparison to uncoated muffins. Antioxidant activity and total plate count of clove oil NE coated muffins were significantly higher than cinnamon oil NE coated muffins and while both the NE coating also effectively facilitated in extending the shelf life up to 6days without any quality deterioration, which facilitated their use as a functional ingredient.

Antimicrobial behavior and mechanism of clove oil nanoemulsion.

Antimicrobial behavior and mechanism of clove oil nanoemulsion.

Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities

AbstractThe therapeutic properties of clove oil are well known, however, the pungent nature, chemical instability, and low water solubility impose limitations in harnessing its potential. Nanoemulsion of clove oil (CON) was prepared to overcome the above constraints. The non‐ionic surfactants Tween 20, 40, and 80 with hydrophile lipophile balance (HLB) of 16.7, 15.6, and 15, respectively, were used to stabilize CON. The creaming index of CON prepared with Tween 20, 40, and 80 was 22.75, 17.5, and 1.5%, respectively, after 8 days of storage at room temperature. Tween 20 and 40 produced particles &gt;300 nm while Tween 80 produced ~150 nm. Transmission electron microscopic image of spray‐dried CON (with Tween 80) showed particles in the range of 150–190 nm. The in vitro release studies showed 76 and 42% cumulative release of CON and native clove oil (NC), respectively, at pH 7.4. The cellular toxicity of CON was significantly reduced by fourfold compared to NC at a concentration of 60 μg/ml when tested on CaCO2 cells. Similarly, hemolytic activity on red blood cells revealed less than 10% hemolysis. In addition, CON also exhibited higher in‐vitro antioxidant compared to NC as shown by 1, 1‐diphenylpicrylhydrazyl and 2,2′‐azino‐di‐[3‐ethylbenzthiazoline sulfonate (6)] (ABTS) radical scavenging activity.Practical applicationThe study highlights the development of clove oil nanoemulsion using cost‐effective, commercially important polysaccharide (maltodextrin) and surfactant for increased bioactivity. The spray‐dried nanoparticle showed high retention (95%) of oil and small particle size even after 1 month of storage at room temperature. The biological studies signify the compatibility of nanoparticle for its nutraceutical applications because of its low cytotoxicity (fourfold reduction), hemolytic activity, and high anti‐oxidant property. The clove oil nanoparticles can replace the clove oil/microencapsulated particles in food product development due to their high water dispersibility and loading content (25% on dry weight basis).

The effect of carboxymethyl cellulose coating incorporated with clove oil nanoemulsion on quality of shrimp (Litopenaeus vannamei) during refrigerated storage.

The quality of shrimp, as high perishable seafood, can readily be effected by microbial, chemical, and physical alterations during storage. In this study, carboxymethyl cellulose (CMC) coating either alone or incorporated with variable levels of clove oil nanoemulsion (CNE) (10, 20, and 30 mg/ml) was developed to sustain the quality of shrimp during refrigerated storage. Changes in microbial, chemical, textural, color (L*, a*, b*), and sensory parameters of the studied groups evaluated through distinct experimental approaches. Based on our results, the pattern of increase in the aerobic plate count (APC) and psychrotrophic bacterial count (PBC) in different groups were reported as the following order: CMC + CNE < CMC < control. Also, CMC and CNE integration decreased the upward trend of total volatile basic nitrogen (TVB-N), and thiobarbituric acid reactive substances (TBARS) compared to that of the control group during storage at 4°C for 10 days (P<0.05). During the storage period, the decrease of the L* value in the control group was greater than the others; whereas, the lowest a* and b* values were observed in this group (P<0.05). Besides, the textural and sensory properties of coated shrimp were significantly more acceptable (P<0.05). Integration of CNE in CMC coating promisingly improves the quality of shrimp during refrigerated storage.

Inhibitory effect of clove oil nanoemulsion on fumonisin isolated from maize kernels

Fumonisins are an important class of mycotoxin due to its wide distribution, health hazard, and economic significance. In the current study, clove oil nanoemulsion was investigated to reduce the load of fumonisin from the maize kernels. The fabricated nanoemulsion of 103 nm size range efficiently reduced the growth of Fusarium proliferatum sp. and HPLC data confirms the reduction in the load of fumonisin B1 and B2 to the maximum recommended level by European Union. Further, clove oil nanoemulsion was electrosprayed to form a ready to use formulation with a core shell matrix. Denatured whey protein has been used as wall material and 96.25% encapsulation efficiencywas achieved. . The improved antifungal activity on maize kernel and ready-to-use prototype has future possibilities to be scaled up at industrial scale. Thus, the encapsulated material can be used as a preventive measure to reduce the fumonisin load during storage conditions.