Cloudy Apple Juice Research Articles

COX-2/iNOS regulation during experimental hepatic injury and its mitigation by cloudy apple juice

A number of enzymes and transcription factors have been correlated with disease etiology. In this study, involvement of cyclooxygenase-2 and inducible-nitric oxide synthase is examined during diethylnitrosamine (DEN)-induced hepatic injury and cloudy apple juice (CAJ) supplementation. Liver injury was administered in rats by single dose of DEN (10 ml/kg bwt of 1% DEN), while 10 ml/kg bwt CAJ daily was given after 2 h of latency in DEN-treated animals for two weeks. CAJ was characterized by HPLC and subsequently examined for antioxidant power. During the course of treatment liver function, collagen (hydroxyproline), malondialdehyde, protein oxidation, antioxidant enzymes, ATPases, nitrite levels were investigated along with liver histopathology and electron microscopy. COX-2 and iNOS proteins were also localized in liver specimens. The results demonstrated rich polyphenols and antioxidant activity in CAJ. CAJ supplementation significantly restored liver biochemistry and anatomy as revealed by the refurbished investigated parameters. CAJ treatment also declined COX-2 and iNOS activities in injured animals. Electron microscopy demonstrated rejuvenated hepatocytes, Kupffer cells, RER, mitochondria and nucleus in CAJ supplemented animals. The novel outcomes of this study suggest that CAJ potentiates hepatoprotection by stimulating antioxidant power and regulating the COX-2 and iNOS proteins in the liver during experimental liver injury.

Effect of cold plasma on polyphenol oxidase inactivation in cloudy apple juice and on the quality parameters of the juice during storage

Direct cold plasma treatment has been investigated as an alternative non-thermal technology as a means of maintaining and improving quality of fresh cloudy apple juice. Process variables studied included type of plasma discharge, input voltage and treatment time on polyphenol oxidase (PPO) inactivation. Spark discharge plasma at 10.5 kV for 5 min was the best treatment, with near total inactivation of PPO achieved, although good PPO inactivation was also recorded using shorter treatment times. Residual activity (RA) of PPO was 16 and 27.6% after 5 and 4 min of treatment respectively. This PPO inactivation was maintained throughout the storage trials, but decreased with samples treated for a shorter time. Plasma treatment improved key quality parameters of Golden delicious cloudy apple juice, with retention of critical quality parameters during extended storage trials. Color was the most noticeable change, which was enhanced with retention of a greener color. An increase of 69 and 64% was obtained in the total phenolic content after 4 and 5 min of treatment, respectively. Therefore, cold plasma was demonstrated to be a good alternative to traditional heat treatments for enhanced quality retention of fresh cloudy apple juice and over its storage.

Filter aid selection allows modulating the vanadium concentration in beverages

This study reports a clear-cut relationship of the technological use of specific filter aids with highly variable vanadium levels in beer, wine, and fruit juices. First, the previously reported broad range of vanadium levels was confirmed in 68 commercial beverages by ICP-MS. Since cloudy apple juices exhibited significantly lower vanadium amounts than clear apple juices, filter aids used for clarification were analyzed and found to contain substantial and highly variable amounts of vanadium, particularly in all analyzed diatomite (38–368 mg vanadium per kg filter aid), but not in perlite products (<4 mg/kg). Subsequent pilot-scale precoat filtration experiments (170 L/batch) proved vanadium to be released from diatomite (Kieselguhr), increasing its levels from ca. 2.1–2.6 µg/kg unfiltered to 27–201 µg/kg filtered juice, depending on the use of diatomites high or low in vanadium. Thus, filter aid selection was shown to modulate the vanadium concentrations in clarified beverages.

The effect of organic and conventional fruit cultivation system on mineral composition and sensory properties of cloudy apple juice

The aim was to determine the differences in mineral composition and sensory properties of cloudy apple juice depending on apple cultivation system. Three indigenous Estonian cultivars were chosen on condition that the same cultivars would be grown in organic and conventional orchards at the same geographical location. ’Krameri tuvioun’ and ’Talvenauding’ apples were harvested from old (over 30 years) low maintenance orchard and ‘Krista’ apples from 10-years old experimental orchard. 100 kg of apples per cultivar and cultivation system were stored at +3±2°C for two months and then pressed into juice using Lancman waterpress. Total soluble solids (TSS), titratable acidity (TA) and content of P, K, Ca and Mg were determined from apples and from juice. Descriptive sensory analysis of apple juice was carried out by a trained sensory panel. ’Krameri tuvioun’ and ’Talvenauding’ apples and respective juices from old organic orchard had significantly higher content of P, K and Mg compared to the conventional counterparts. Mineral composition of ’Krista’ apples from younger well- maintained organic orchard and respective juice was not significantly different from conventional ones except for P concentration, which was higher compared to conventional apples. Organic apples from old orchard had higher TSS compared to conventional ones, but this difference was no more significant in juice. Sensory properties of juices were most of all affected by cultivar: ‘Krameri tuvioun’ juice was sweeter and less sour compared to ‘Talvenauding’ and ‘Krista’. Cultivation system did not affect the sweetness, sourness as well as fresh- and apple flavour. Our results indicate that even though the old organic apple orchards do not produce high quality apples for fresh consumption, cloudy apple juice made from those apples is better source of valuable mineral elements for humans than apple juice pressed from conventional or well-maintained organic orchards.

High‐power ultrasound altered the polyphenolic content and antioxidant capacity in cloudy apple juice during storage

The aim was to investigate the influence of high-power ultrasound treatment (HPU) on polyphenolic stability and antioxidant capacity in cloudy apple juice during 7 days of storage at 4°C. HPU (100 W, 30 kHz frequency) was operated at: (i) amplitude 40 versus 80%, (ii) the probe diameter 7 versus 10 mm, and (iii) treatment time of 3, 6, and 9 min. Total phenols (TP), total flavan-3-ols (TFL), and in vitro antioxidant capacity (DPPH and FRAP) were determined spectrophotometrically. Findings revealed that HPU significantly decreased TP, TFL, and antioxidant capacity in the samples. However, results indicated that examined sonication parameters, represented as the probe diameter and treatment time, significantly contributed to the stability of TFL. With respect to shelf-life, the greatest stability of both, polyphenols and antioxidant capacity, was observed in all un-treated samples, whereas significant lower amounts of TP, TFL, DPPH, and FRAP were found in sonicated samples. Practical applications The global fruit juice market is constantly growing due to the increased consumer preferences toward healthier foods. Consumption of fruit juices, like cloudy apple juice, is a convenient way to get more servings of fruits per day for increased health benefits from dieting. Thermal processing of this juice may negatively influence the nutritive and biological value, so high-power ultrasound technology (e.g., nonthermal processing), could be useful tool to ensure stability during cold storage. Therefore, enhanced phenolic and antioxidant stability obtained with innovative nonthermal approach is directly related with economic and nutritive cost-benefits of industrial processing.

Comparative evaluation of flavour and nutritional quality after conventional and microwave-assisted pasteurization of cloudy apple juice

Novel processing technologies have been under study to improve pasteurization and to meet consumer demands for safe foods with fresh-like characteristics. Twelve processing conditions using two heating technologies (microwave and conventional) were carried out in a pilot-scale unit and an evaluation of flavour (sugar, acid and volatile) and nutritional (phenolic compounds and antioxidant capacity) attributes was performed in cloudy apple juice samples with similar holding conditions. Volatile profiles of the microwave-pasteurized juice were more similar to the non-pasteurized juice compared with conventionally pasteurized juices using multivariate analysis. Sugar-acid ratios were not different between fresh and pasteurized juices. In general, changes in the phenolic contents of pasteurized samples were independent of the processing technology, but increased during thermal treatment due to the extraction from the juice material in suspension. Outcomes of this study suggested the potential of microwave processing technology to retain the fresh-like qualities after thermal processing because of the shorter heating time.

Effects of ascorbic acid on patulin in aqueous solution and in cloudy apple juice.

Degradation of the mycotoxin patulin (PAT) and the generation of (less toxic) breakdown products, such as (E/Z)-ascladiol (ASC-E/Z) and desoxypatulinic acid (D-PAT), can occur due to chemical, physical and biological treatments. Our study focused on the chemical degradation of PAT in the presence of ascorbic acid (AA) both for pure PAT standard in acidified aqueous solution and for PAT-contaminated cloudy apple juice (CAJ) (obtained via addition of apple mash produced from apples inoculated with Penicillium expansum). Within this framework, different concentrations of AA were evaluated, as well as the presence/absence of oxygen and different storage temperatures. In order to do so, an in-house methodology allowing a good separation of PAT from its reaction and breakdown products was optimized first. The highest PAT reduction (60%) in CAJ with an initial PAT concentration of 100μg/kg and 0.25% (w/v) AA was achieved after 6days of incubation at 22°C in the presence of oxygen. It was also found that the treatment by AA resulted in the generation of degradation products less toxic than PAT (such as (E/Z)-ASC). In conclusion, AA used to improve numerous product quality aspects (e.g. colour (less browning), nutritional value, etc.) and considered as a safe food additive (Food and Drug Administration (FDA) (1999)) has an effect on PAT degradation. It was shown that such degradation generated less toxic compounds in the presence of oxygen. In view of consumers' safety, fortification of apple juice (and possibly apple-based products) with AA could be helpful within an integrated system to ensure products with low levels of patulin. The optimum conditions for such an approach within a legal and practical point of view need to be further explored.

Enzyme inactivation and evaluation of physicochemical properties, sugar and phenolic profile changes in cloudy apple juices after high pressure processing, and subsequent refrigerated storage

AbstractThe effect of high pressure processing (HPP) (200–600 MPa/5–45°C/1–15 min) on the enzyme activity and some quality parameters of cloudy apple juice during subsequent storage (4°C for 12 weeks) was investigated. Statistical analysis showed that pressure, temperature, and time had a significant effect (p &lt; 0.05) on tissue enzyme activity, decreasing the activity of polyphenol oxidases (PPO) and peroxidases (POD). No significant changes in physicochemical parameters (pH, total soluble solids, sugars, and vitamin C) were observed after HPP treatments. The main polyphenols detected in apple juice were dichydrochalcones, being phloridzin the predominant (48.8 mg/L), and flavanols, with the highest concentration of (−) epicatechin (20.6 mg/L) followed by (+) catechin (6.4 mg/L) and procyanidin B1 (1.9 mg/L). Moreover, the application of HPP treatment significantly decreased gallic acid, all flavanols and dichydrochalcones. The storage time significantly affected total polyphenol content (TPC) and selected phenolic compounds, but the most stable were TPC.Practical applicationsThis study demonstrated that nutritional quality of cloudy not produced from concentrate apple juice after high pressure processing (HPP) and during subsequent cold storage. HPP significantly influenced on tissue oxidoreductive enzymes decreasing their residual activity below 1 and 33%, respectively, for polyphenol oxidases and peroxidases. The results showed that HPP might be a useful method for preservation apple products, resulting higher color stability due to the inhibition of enzymatic reactions during storage time.

Comparing the impact of high pressure, pulsed electric field and thermal pasteurization on quality attributes of cloudy apple juice using targeted and untargeted analyses

Abstract The impact of low-oxygen spiral-filter press technology combined with thermal pasteurization (TP), pulsed electric field (PEF) and high pressure processing (HPP) on cloudy apple juice quality was investigated immediately after the treatments and after 3 weeks of storage at 4 °C. Based on equivalent levels of microbial safety and desired shelf-life, low and high processing intensities were selected: TP (72 °C/15 s; 85 °C/30 s), PEF (12.5 kV/cm, 76.4 kJ/L; 12.3 kV/cm, 132.5 kJ/L), and HPP (400 MPa/3 min; 600 MPa/3 min). High intensity thermal treatment resulted in a bright, yellowish color which was maintained during storage. PPO and POD activities were largely reduced by high intensity PEF and TP yet showed high resistance to HPP. The highest vitamin C content was provided by fresh juice followed by PEF-treated juices. Due to oxidative degradation reactions, vitamin C of all treated samples significantly decreased during storage. Immediately after processing, high cloud stability values were obtained in all samples; however, cloud stability decreased during storage particularly for HPP juices with high residual PME. No significant changes were observed in pH, titratable acidity, organic acid and sugar content which also corresponded to sweet and sour taste. Results from untargeted volatile profiles showed that esters increased after PEF and were better retained after HPP. Contrary to TP treatment where ester degradation reactions occurred together with the formation of off-flavors. Most of the volatiles decreased during storage which could be linked to oxidation and ester hydrolysis reactions. Industrial relevance Being one of the most popular fruit juices consumed worldwide, cloudy apple juice can still undergo quality changes such as color degradation, cloud loss (fast sedimentation) and flavor changes during processing and storage. This study evaluates the potential of low-oxygen spiral-filter press in combination with different preservation technologies to obtain a maximal quality of cloudy apple juice. Results showed that high intensity thermal pasteurization can effectively inactivate quality-degrading enzymes, therefore it is useful to obtain an optimal cloudy apple juice product in terms of color and cloud stability. Although HPP has minimal impact on aroma of the juice, shelf-life of the juice may be limited due to incomplete enzyme inactivation. In the case of PEF treatment, thermal effects may contribute to maintain apple juice quality.

Use of near infrared spectroscopy and chemometrics to evaluate the shelf-life of cloudy sonicated apple juice

Fresh products, such as cloudy apple juice, could be preserved from early spoilage through the application of non-thermal processes such as sonication. However, shelf-life analyses based on microbiological and sensory evaluations are expensive and time consuming. Few studies have applied near infrared spectroscopy to evaluate the quality and decay of apple juices. Here, a feasibility trial was conducted to study the spectral behaviour at 1300–2500 nm combined with chemometric approaches. The shelf-life was monitored during two experiments, a challenge test with juices inoculated with spoilage yeasts (inoculated non-sonicated (INS)) and then submitted to sonication treatments (inoculated sonicated (IS)), and a storage test to evaluate the spoilage on non-inoculated juices (non-inoculated non-sonicated (NINS)) and sonicated non-inoculated juices (non-inoculated sonicated (NIS)). These experiments were investigated at six different refrigeration times 7, 14, 21, 28 and 60 days. Two functions were modelled to describe the behaviours of the first principal component according to the storage time. In agreement with a previous chemical and sensory evaluation, this approach allowed us to highlight shelf-life end points of 7 and 14 days for non-sonicated and sonicated samples, respectively. Three different models were evaluated for classification purposes: (1) sonicated versus non-treated samples, (2) end-point shelf-life evaluation at seven days for the NINS and INS juices and (3) end-point shelf-life discrimination at 14 days for IS and NIS samples. A partial least square-discriminant analysis enabled a group classification with accuracy values ranging from 0.63 to 1.00. The application of a variable importance in projection index to interpret the wavelengths of the spectral features suggests a contribution of organic acids and lipids to the prediction of decay. A canonical discriminant analysis provided a clearer separation of samples according to the storage time, especially in relation to the two time thresholds of 7 and 14 days.

Modeling of rheological properties of cloudy apple juice using master curve

ABSTRACTThe effect of temperature and concentration on the rheological behavior of pasteurized apple juice has been studied using a rotational rheometer at shear rates ranging from 0.5 to 12 s−1. The rheological properties were measured at different conditions changing from 10°C to 60°C and from 15°Brix to 40°Brix. On the basis of the obtained results, flow curves were prepared and consistency coefficients and flow indexes were determined. The superposition technique was applied to model behavior of apple juice. The obtained master curve is very important for the industry because it shows the apple juice in the whole range of temperature and soluble solid content. The influence of temperature on consistency coefficient was well described by the Arrhenius equation with activation energy values between 23.3 and 33.6 kJ⋅mol−1. A single equation was proposed for describing the combined effect of temperature, concentration, and shear rate on shear stress at constant flow behavior index. The model can be useful for the easy comparison of the rheological properties of different fluid food.

Cultivar classification of cloudy apple juices from substandard fruits in China based on aroma profile analyzed by HS-SPME/GC-MS

Aroma profiles of cloudy apple juices from substandard apple with different cultivars were investigated by headspace solid phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). 38 volatile compounds (including esters, alcohols, acids, aldehydes, terpenes, etc.) were identified. 30 Cloudy apple juices samples of 5 cultivars in China were characterized and classified by different chemometric analysis based on volatile compounds. Discrimination models were established based on volatile profiles of cloudy apple juices by chemometric techniques including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and stepwise linear discriminant analysis (SLDA). Satisfactory discriminations were achieved by SLDA based on volatile compounds according to cultivar with prediction accuracy of 93.3%. The results suggested that SLDA could be used to discriminate substandard cloudy apple juices based on the volatile compounds. The obtained results could also provide data basis for selecting substandard apple fruits of different cultivars for juice production with better aroma profile.

124 - Anti-genotoxic effects of fruit and vegetables and their constituents

A diet rich in fruit and vegetables may decrease the risk of cancer. Decrease of endogenous or exogenous genotoxicity by fruit and vegetables may be partly responsible for this effect. Using the Comet assay in a number of in vivo studies, we attempted to identify the fruit and vegetable species, their extracts or isolated compounds able to decrease endogenously or exogenously-induced DNA damage. We found that consumption of apples protected against formation of endogenously oxidized purine bases and increased capacity to protect DNA in lymphocytes in humans. Cloudy apple juice (750 ml, 2 weeks) also showed an increased capacity to protect DNA in humans. Using the1,2-dimethylhydrazine-rat model of carcinogenesis, we demonstrated the anti-genotoxic and anti-carcinogenetic effects of cloudy apple juice but not their fractions. We also observed a significant reduction of irinotecan-induced DNA-strand breaks after a pre-treatment with isolated anthocyans (cyanidin, cyanidin-3-glucoside) and a blackberry extract in rats and demonstrated that daily consumption of tomato oleoresin containing about 15 mg of lycopene decreased endogenous DNA damage. In contrast, 200 g broccoli for 4 weeks or 330 ml carrot juice for 2 weeks had no effect in humans. Also, consumption of 8 servings (about 800 g) of different commonly consumed fruit and vegetables for 4 weeks showed no effect on DNA damage in lymphocytes in humans. These observations indicate that the kind of fruit or vegetable (e.g. apples, blackberries, tomato products) rather than the total amount of different commonly consumed fruit and vegetables is of essential importance for the anti-genotoxic effects in humans.

Effects of Acidity on the Stabilities of Cloudy Apple Juice

Acidity is an important influence factor for juice stability. In this study, the effects of acidityon stability of cloudy apple juice were investigated. The stability indexes, such as, turbidity, cloudvalue, cloud stability, and particle size distribution (PSD) were measured at pH 2.0, 2.5, 3.0 and 3.5,along with the untreated juice (pH 3.8) as control group. The results showed that, pH has significanteffects on turbidity, chrome (C*), cloud value, and D50, D90 in PSD of cloudy apple juice. The juicehad a better stabilization at pH 2.5. At this pH condition, the turbidity, cloud value, cloud stability ofjuice was the best. In addition, at pH 2.5, the color of the juice was better, the particles were smaller,and the particle sizes were more uniform. By this study, a theoretical reference could be provided forimproving stability of cloudy apple juice and extending juice shelf life.

Effect of xanthan gum on typicity and flavour intensity of cloudy apple juice

Effect of xanthan gum on typicity and flavour intensity of cloudy apple juice

Modeling of time-temperature history and enzymatic inactivation of cloudy apple juice in continuous flow microwave assisted pasteurization

Enzyme inactivation can be the target of a pasteurization process of fruit juices. Fresh cloudy apple juice was subjected to continuous flow microwave assisted pasteurization in a pilot scale unit at three processing temperatures (70°C, 80°C and 90°C), two flow rate levels and two heating systems (conventional and focused microwave). A heat transfer model was developed and combined with mean residence time information to provide the average time-temperature history of the product. Inactivation kinetics (first order with two fractions) was used to calculate the integrated lethality. The thermal model was validated with measurements along the product path and the final residual activity was compared with the model prediction. Good results were obtained for polyphenol oxidase (PPO) and peroxidase (POD) but not for pectin methylesterase (PME), which showed to be the most resistant enzyme. Focused microwave heating resulted in a high heating rate with a temperature profile close to the ideal of instantaneous heating, consequently reducing the lethality contribution from the heating step. The results show the importance of modeling and simulation in process analysis.

Enzymatic, physicochemical, nutritional and phytochemical profile changes of apple (Golden Delicious L.) juice under supercritical carbon dioxide and long-term cold storage

The impact of supercritical carbon dioxide (SCCD) (10–60 MPa/45 °C/30 min) and subsequent 10 weeks storage at 4 °C on polyphenol oxidase (PPO), peroxidase (POD) activities, phenolic profile, vitamin C, sugars, physicochemical properties of cloudy apple juices was investigated.No significant changes in sugars and total polyphenols were observed, whereas significant degradation (≈28%) of vitamin C and individual polyphenols (≈18%) was noted after SCCD treatment. After 4 weeks storage only 34% of vitamin C was retained and no vitamin C was detected after this time. Ten weeks of storage caused hydrolysis of sucrose in 15%, whereas degradation of individual polyphenols ranged from 43 to 50% depending on the pressure applied. The highest pressure was applied the highest retention of polyphenols was observed. The lightness of juice significantly increased by 15% after SCCD and decreased during storage. Moreover, the synergistic effect of both enzymes with chlorogenic acid and catechol was found.

Host: Microbiome co-metabolic processing of dietary polyphenols – An acute, single blinded, cross-over study with different doses of apple polyphenols in healthy subjects

Apples are one of the most commonly consumed fruits and their high polyphenol content is considered one of the most important determinants of their health-promoting activities. Here we studied the nutrikinetics of apple polyphenols by UHPLC-HRMS metabolite fingerprinting, comparing bioavailability when consumed in a natural or a polyphenol-enriched cloudy apple juice. Twelve men and women participated in an acute single blind controlled crossover study in which they consumed 250 mL of cloudy apple juice (CAJ), Crispy Pink apple variety, or 250 mL of the same juice enriched with 750 mg of an apple polyphenol extract (PAJ). Plasma and whole blood were collected at time 0, 1, 2, 3 and 5 h. Urine was collected at time 0 and 0–2, 2–5, 5–8, and 8–24 h after juice consumption. Faecal samples were collected from each individual during the study for 16S rRNA gene profiling. As many as 110 metabolites were significantly elevated following intake of polyphenol enriched cloudy apple juice, with large inter-individual variations. The comparison of the average area under the curve of circulating metabolites in plasma and in urine of volunteers consuming either the CAJ or the PAJ demonstrated a stable metabotype, suggesting that an increase in polyphenol concentration in fruit does not limit their bioavailability upon ingestion.Faecal bacteria were correlated with specific microbial catabolites derived from apple polyphenols. Human metabolism of apple polyphenols is a co-metabolic process between human encoded activities and those of our resident microbiota. Here we have identified specific blood and urine metabolic biomarkers of apple polyphenol intake and identified putative associations with specific genera of faecal bacteria, associations which now need confirmation in specifically designed mechanistic studies.

Inactivation Kinetics of Pectin Methylesterase, Polyphenol Oxidase, and Peroxidase in Cloudy Apple Juice under Microwave and Conventional Heating to Evaluate Non-Thermal Microwave Effects

Continuous-flow microwave pasteurization provides important advantages over conventional heat exchangers such as fast volumetric heating, lower tube surface temperature, and possible non-thermal effects that enhance enzymatic and bacterial inactivation. Conventional and microwave-assisted inactivation of pectin methylesterase (PME), polyphenol oxidase (PPO), and peroxidase (POD) in cloudy apple juice were investigated to evaluate non-thermal effects. Experiments were conducted to provide uniform heating with accurate temperature acquisition and similar temperature profiles for conventional and microwave treatments. A two-fraction first-order kinetic model was successfully fitted to the data in a procedure that took into account the whole time-temperature profile instead of assuming isothermal conditions. Predicted inactivation curves for pasteurization at 70 and 80 °C of the cloudy apple juice showed that PME has the highest thermal resistance (residual activity of 30% after 250 s at 80 °C) and that there was no evidence of non-thermal microwave effects on the inactivation of these enzymes.

The potential of kiwifruit puree as a clean label ingredient to stabilize high pressure pasteurized cloudy apple juice during storage

In the fruit juice industry, high pressure (HP) processing has become a commercial success. However, enzymatic browning, cloud loss, and flavor changes during storage remain challenges. The aim of this study is to combine kiwifruit puree and HP pasteurization (600 MPa/3 min) to stabilize cloudy apple juice during storage at 4 °C. A wide range of targeted and untargeted quality characteristics was evaluated using a multivariate approach. Due to high ascorbic acid content and high viscosity, kiwifruit puree allowed to prevent enzymatic browning and phase separation of an apple-kiwifruit mixed juice. Besides, no clear changes in organic acids, viscosity, and particle size distribution were detected in mixed juice during storage. Sucrose of apple and mixed juices decreased with glucose and fructose increasing during storage. The volatile changes of both juices behaved similar, mainly esters being degraded. Sensory evaluation demonstrated consumer preferred the aroma of mixed juice compared to apple juice.