Abstract. Over the years, the problem of dissipation rate of turbulent kinetic energy (TKE) in stable stratification remained unclear because of the practical impossibility to directly measure the process of dissipation that takes place at the smallest scales of turbulent motion. Poor representation of dissipation causes intolerable uncertainties in turbulence-closure theory and thus in modelling stably stratified turbulent flows. We obtain a theoretical solution to this problem for the whole range of stratifications from neutral to limiting stable; and validate it via (i) direct numerical simulation (DNS) immediately detecting the dissipation rate and (ii) indirect estimates of dissipation rate retrieved via the TKE budget equation from atmospheric measurements of other components of the TKE budget. The proposed formulation of dissipation rate will be of use in any turbulence-closure models employing the TKE budget equation and in problems requiring precise knowledge of the high-frequency part of turbulence spectra in atmospheric chemistry, aerosol science, and microphysics of clouds.