Abstract. Cloud remains one of the largest uncertainties in weather and climate research due to the lack of fine-resolution observations of cloud vertical structure (CVS) on a large scale. In this study, near-global CVS is characterized by high-vertical-resolution twice-daily radiosonde observations from 374 stations over land, which are distributed in Europe, North America, East Asia, Australia, the Pacific Ocean, and Antarctica. To this end, we initially develop a novel method to determine CVS, by combining both the vertical gradients of air temperature and relative humidity (RH) and the altitude-dependent thresholds of RH. It is found that the cloud base heights (CBHs) from radiosondes have a higher correlation coefficient (R= 0.91) with the CBHs from a millimeter-wave cloud radar than those from the ERA5 reanalysis (R= 0.49). Overall, cloudy skies occur 65.3 % (69.5 %) of the time, of which 55.4 % (53.8 %) are one-layer clouds at 00:00 (12:00) UTC. Most multi-layer clouds are two-layer clouds, accounting for 62.2 % (61.1 %) among multi-layer clouds at 00:00 (12:00) UTC. Geographically, one-layer clouds tend to occur over arid regions, whereas two-layer clouds do not show any clear spatial preference. The cloud bases and tops over arid regions are higher compared with humid regions albeit with smaller cloud thickness (CT). Clouds tend to have lower bases and thinner layer thicknesses as the number of cloud layer increases. The global-mean CT, CBH, and cloud top height (CTH) are 4.89 ± 1.36 (5.37 ± 1.58), 3.15 ± 1.15 (3.07 ± 1.06), and 8.04 ± 1.60 (8.44 ± 1.52) km above ground level (a.g.l.) at 00:00 (12:00) UTC, respectively. The occurrence frequency of clouds is bimodal, with lower peaks between 0.5 and 3 km a.g.l. and upper peaks between 6 and 10 km a.g.l. The CBH, CTH, and CT undergo almost the same seasonality; namely, their magnitudes in boreal summer are greater than in boreal winter. As expected, the occurrence frequencies of clouds exhibit pronounced diurnal cycles in different seasons. In boreal summer, clouds tend to form as the sun rises and the occurrence frequencies increase from morning to late afternoon, with the peak in the early afternoon at the altitude of 6–12 km a.g.l., while in boreal winter, clouds have peak occurrence frequencies in the morning. The relations between surface meteorological variables and moisture with CBH are investigated as well, showing that CBHs are generally more significantly correlated with 2 m relative humidity (RH2 m) and 2 m air temperature (T2 m) than with surface pressure and 10 m wind speed. Larger T2 m and smaller RH2 m always correspond to higher CBH. In most cases CBHs are negatively correlated to soil water content. The near-global CVS obtained from high-vertical-resolution radiosondes in this study can provide key data support for improving the accuracy of cloud radiative forcing simulation in climate models.