The use of a Closed Loop Calibration System (CLCS) for calibration of several portable models of MIRAN infrared gas analyzers has been recommended by the manufacturer. The purpose of this study was to determine the extent of organic vapor loss due to wall adsorption that may result in erroneous light absorbance data. Two MIRAN-1A gas analyzers that had passed the performance and wavelength accuracy tests were connected in series to a bellows pump to form a CLCS. Fourteen organic solvents, with vapor pressure: 0.43-350 mm Hg (at 25°C), were used in the study. Increments (0.5-5 µL) of high purity solvents were introduced into the CLCS via a septum. Simultaneous gas chromatographic (G.C.) analyses were performed on aliquots of gas mixture drawn between consecutive injections of solvent liquid to determine the actual concentrations of organic vapors within the CLCS. Absorbance data were recorded separately for each gas analyzer. Two regression lines were established for each organic vapor: (i) between expected (calculated) concentration vs. absorbance, and (ii) between actual concentration (determined by G.C.) and absorbance. The two regression lines were compared statistically. The differences in absorbance values between the two lines at 1/2, 1, and 2 times the OSHA Permissible Exposure Level (PEL) were insignificant for nine organic solvents with vapor pressure: 15-350 mm Hg (at 25°C). However, the absorbance values from the two lines at the above mentioned concentrations were significantly different for five organic solvents with vapor pressure: 0.43-8.3 mm Hg (25°C). In the latter group, the slopes of the lines: i and ii were almost equal; however, there were significant differences between the intercepts. In the absence of leaks from the CLCS, the unidirectional differences between the intercepts (bii>bi) resulted from the vapor loss due to wall adsorption. There was a significant correlation between the degree of wall loss within the CLCS and the decline in vapor pressure. The extent of errors (overestimation of concentration) caused by using calibration curves established by CLCS were between 4.2 to 29.3% at OSHA PEL. It was concluded that even though, from the control standpoint, such reading errors would not cause any harm to (if not benefit) the workers, the use of CLCS would not be recommended for solvents with vapor pressure lower than 15 mm Hg (at 25°C) if maximum accuracy in measurement is aimed.