Problems with glycemic and lipid changes such as diabetes mellitus and dyslipidemias are increasingly affecting the population and involve the risk of developing cardiovascular and metabolic disorders. Persistent hyperglycemia is associated with chronic micro and macrovascular complications, increased morbidity, reduced quality of life, and increased mortality rate. Therefore, it is important to perform routine laboratory tests in order to diagnose such disorders early, which can be observed through laboratory markers. The purpose of this study was to analyze the tests requested for the evaluation of the lipid and glycemic profile of patients attended at the Ana Bezerra University Hospital, located in Santa Cruz, Rio Grande do Norte. This retrospective documentary epidemiological study sought to analyze the amount of tests required for the evaluation of the lipid and glycemic profile in the database of the Laboratory of Clinical Analysis of the Hospital Universitário Ana Bezerra from June 2017 to June 2018. During this period, 17660 exams were requested to evaluate the glycemic profile and 34266 tests to evaluate the lipid profile. Fasting plasma glucose and total cholesterol were the most requested.