Objectives: This study aimed to assess the knowledge of nursing students regarding Standard Precautions at Duy Tan University in 2022. Participants and methods: A cross-sectional design was employed, involving 272 second, third, and fourth-year nursing students who participated in clinical internships from February and July 2022. A 56-item questionnaire based on the Ministry of Health’s SPs guidelines was used to evaluate students’ knowledge. Results: The findings revealed that the overall knowledge level of SPs among nursing students was 60.3%, with significant differences across academic years. Fourth-year students demonstrated superior knowledge compared to second- and third-year students. Factors such as the number of internship facilities, academic performance, participation in SPs-related programs, and the diversity of information sources were found to significantly influence students’ knowledge of SPs. The study identified knowledge gaps, particularly in personal protective equipment use, medical instrument handling, and environmental sanitation. Conclusions: It is recommended that nursing curricula include more comprehensive and systematic training on SPs and infection control practices, incorporating both theoretical and hands-on components, to improve students’ competency. Moreover, educational institutions should leverage digital media and organize regular SPs seminars to enhance students’ knowledge and preparedness for clinical practice. This research highlights the need for continuous education and training to ensure nursing students are well-equipped to prevent infections and promote patient safety in healthcare settings.
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