Japanese horseradish [Wasabia japonica (Miq.) Matsumara or Eutrema wasabi (Siebold) Maxim.], is a vegetable species originated from Japan. Currently, wasabi is grown in many other countries across the world such as: New Zealand, Taiwan, South Korea, Israel, Brazil, Thailand, Columbia, Canada, USA, Great Britain and China. The crop has never been cultivated commercially in Poland. The main aim of the present study was to find out if the crop can be grown commercially in Poland and to establish the chemical contents of roots produced in Poland and Japan. In previous studies, it was noted that wasabi might be grown under polycarbonate cover in Poland. The chemical evaluation included measuring the amount of dry matter, polyphenols, antioxidant potential, reducing substances, total proteins, starch and raw fiber. The roots produced in Poland had more dry matter (30.46%), less total proteins (12.14%) and reducing substances (43.64 mg g-1) than the Japanese ones. Starch and raw fiber contents were on the same level in the roots produced in both countries. The antioxidant potential and polyphenols in the Japanese roots (6.11 mg g-1 and 7.83 mg g-1 respectively)were higher than in the Polish ones (1.45 mg g-1 and 3.25 mg g-1 respectively). In the climatic conditions of Poland, it might be possible to produce wasabi roots under the polycarbonate cover with their chemical contents at least in part similar to the original roots produced in Japan.