Study regionChao Phraya River Basin (CPRB), the predominant basin located in Thailand. Study focusThis study aims to ascertain the difference between spatial bias heterogeneity of streamflow in large river basins such as CPRB for a robust analysis. The upstream major dams and the outlet of the basin were examined with two-step bias correction and compared with a more practical bias correction only at the outlet of the basin. The former clarified that, due to the large effect of downstream bias, the upstream bias effect was considered negligible thus the two approaches resulted in similar future projections in the CPRB. Through this comparison, streamflow bias in the past and future climate experiments was corrected considering its spatial characteristics for robust assessments of quantitative impacts of climate change. New hydrological insights for the regionA + 4 K warmer climate will increase the frequency of the 2011 flood in CPRB and enhance 100-year flood peak discharge by 1.1–1.6 times than the past climate (1961–2010). The future flood in the basin, which starts predominantly in September in the present climate, is likely to begin in September and August equally with a prolonged duration of floods around 10–50 days. The study region is likely expected to experience elevated flood volume, earlier flood occurrence, and longer flood duration which indicates that forthcoming floods will be more rigorous.