Research of the biocide action of streptomycete-antagonist metabolites on the causative agent of bacterial canker of tomato and on the host-plant fertility is the actual question. Aim. To determine the action of metabolites of Streptomyces globisporus 3-1 and Streptomyces cyanogenus S136 on the phytopathogen Clavibacter michiganensis 102 in vitro and on seed germination, growth and yield of tomatoes in vivo. Methods. Microbiological methods in laboratory conditions and descriptive-comparative analysis of growth and development of tomatoes in hothouse conditions were used. Results. S. globisporus 3-1 and S. cyanogenus S136 metabolites inhibited the growth of the phytopathogen C. michiganensis 102 with the formation of clean zones 45 and 35 mm in vitro, respectively. Pre-sowing treatment with metabolites of S. globisporus 3-1 and S. cyanogenus S136 of tomatoes Lana seeds reduced germination by 27% for both and led to defects in leaf shape and plant sterility, respectively. Treatment by metabolites of both Streptomyces strains of phytopathogen infected seeds did not improve germination, growth, and yield compared to grown plants from infected seeds, though it were visually not defective, sterile and with signs of bacterial canker of tomato. Conclusion. It was found that metabolites of S. globisporus 3-1 and S. cyanogenus S136 had a biocide action on the phytopathogen C. michiganensis 102 in vitro and on Lana tomatoes in hothouse conditions.