ABSTRACT Earthworms are a major component of soil fauna communities with positive effects on soil chemical, biological and physical processes. A study was carried out to investigate the medium-term effects of cultural practices on earthworm communities in an agricultural field. Data were collected in the 2018/2019 cropping season from a six-year-old experiment with tillage system, fertiliser application rate and weeding intensity as the main, sub- and sub-subplots, respectively. Lumbricus (34.4%) and Diplocardia (38.3%) were the dominant genera, while endogeic earthworms (48.4%) dominated the community structure among other earthworm functional groups. Clean weeded plots under the basin planting system had higher Lumbricus abundance (quadruple), genus richness (76.4%) and Shannon diversity index (56.0%) than all other treatments. Inorganic fertiliser application in the conventional tillage system reduced Eisenia abundance and genus evenness by 100.0% and 73.3%, respectively. These results suggest that in minimum tillage systems, frequent hand weeding confers positive effects on earthworms including increased abundance. In conventional tillage systems, application of high doses of inorganic fertiliser is detrimental to earthworm communities. Further studies need to focus on identifying the most sustainable and cost-effective hand weeding frequency for enhanced earthworm diversity and increased crop productivity.