Objectives: The objectives of the research was to evaluate the prevalence of uropathogens, their resistance pattern, and association factors of UTI among diabetic and non-diabetic patients. Material and Methods: A cross-sectional study was conducted during the year Sep-2022 to Jan-2024, among 1048 adult patients from SMIMER (Smimer Hospital and Medical college, Surat) and other private laboratory from Surat area. Using recommended culture methods, Clean-catch midstream urine samples were collected and examined for the presence of uropathogens and their antimicrobial susceptibility pattern. Results: The highest prevalence of uropathogens were Escherichia coli (49%), Klebsiella spp (39.2%) and Candida (fungus) (13.2%). Most of the uropathogens were sensitive to nitrofurantoin, cefixime and amikacine in diabetic and most of the uropathogens were sensitive to meropenem, amikacine and levofloxacin in non-diabetic patients. Whereas resistant to Ampicillin/Sulbactam, Cefuroxime and Co-Trimoxazole (Sulpha/Trimethoprim) in diabetic patients, same as resistant to amikacin and netillin drug in non diabetic patients. Conclusion: The prevalence of UTI and most commonly used antibiotics among diabetic and non-diabetic patients is compared with published paper. Urine analysis and culture should be performed in all diabetic and non diabetic patients. Most common uropathogens is E.coli in both groups.