Event Abstract Back to Event Drug infused clay nanotubes for combinatorial drug delivery Reid Grimes1 and David K. Mills1 1 Louisiana Tech University, Biomedical Engineering, United States Halloysite clay nanotubes (HNTs) are capable of being vacuum-loaded with a variety of different compounds. In addition, it has also been shown that layers deposited through layer-by-layer self-assembly can effectively coat these tubes. In this project, polyelectrolyte (PE) layers were deposited onto the surface of the HNTs, with the addition of a layer of the drug, Rifampicin sequestered within the layers. SEM images were taken to confirm HNT coating. The tubes were further coated to contain and retard the Rifampicin release. The PE coating structure was composed of two distinct bilayers of poly (sodium 4-styrene sulfonate, PSS) with poly (diallyldimethylammonium chloride, PDM) and poly acrylic acid (PAA) with polyvinylpyrrolidone (PVPON). The foundation of the system was comprised of a series of PSS/PDM multi-layers followed layers of PAA/PVPON. Once the foundation was created, the drug Rifampicin was loaded onto the layers. Then a series of PAA/PVPON layers were deposited followed by a final series of PSS/PDM layers. Samples were tested at pH 7 to ensure that the drug was effectively contained and the PE layers were then broken down and the amount of drug retained determined. The system was shown to be stable at neutral pH, a comparison study examined release/retention between pH 7 and pH 5.6. Samples were allowed to stir for five minutes after which an initial reading was taken using UV-Vis spectroscopy. The samples were then allowed to stir for 48 hours undisturbed, after which another reading was taken. Finally, the sample was allowed to stir for an additional 24hrs, and another reading taken. In addition to testing the capped samples, samples were created that were identical, but lacked PAA/PVPON and PDM/PSS multilayers on top of the drug layer and served as the control. SEM microscopy confirmed that individual HNTs have a coating completely covering their surface. At pH 5.6, samples released Rifampicin throughout the trial. However, the amount of drug released was substantially less than that of the control. In the pH 7 trials, the control continuously released large amounts of Rifampicin. However, the capped samples did not release any of the drugs until much later in the release profile. These results show that layers, composed of PSS/PDM and PAA/PVPON, infused with the drug Rifampicin can be created on the surface of HNTs. Additionally, this study shows that the layers are also pH responsive. Rifampicin was used as a model drug in these trials, however due to the nature of the system, various other drugs can be placed within the structure. Current studies investigate the release profile of the system at five-minute intervals for extended amounts of time. Furthermore, a number of other drugs are being loaded and studied in this system. In conclusion, a method for effectively entrapping a drug in the layers created on the surface of HNTs has been created that is also pH responsive. The author's wish to acknowledge the Louisiana Governor's Initiative for their funding support of this project. Keywords: Drug delivery, nanotube, biofunctionalization, Nano/micro particle Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Drug-eluting devices Citation: Grimes R and Mills DK (2016). Drug infused clay nanotubes for combinatorial drug delivery. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02383 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. 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