The motor unit action potentials (MUPs) in an electromyographic (EMG) signal provide a significant source of information for the assessment of neuromuscular disorders. Since recently there were different types of developments in computer-aided EMG equipment, different methodologies in the time domain and frequency domain has been followed for quantitative analysis of EMG signals. In this study, the usefulness of the different feature extraction methods for describing MUP morphology is investigated. Besides, soft computing techniques were presented for the classification of intramuscular EMG signals. The proposed method automatically classifies the EMG signals into normal, neurogenic or myopathic. Also, multilayer perceptron neural networks (MLPNN), dynamic fuzzy neural network (DFNN) and adaptive neuro-fuzzy inference system (ANFIS) based classifiers were compared in relation to their accuracy in the classification of EMG signals. Concerning the impacts of features on the EMG signal classification, different results were obtained through analysis of the soft computing techniques. The comparative analysis suggests that the ANFIS modelling is superior to the DFNN and MLPNN in at least three points: slightly higher recognition rate; insensitivity to overtraining; and consistent outputs demonstrating higher reliability.