Police as an institution that has many divisions and staff that require an information system. In the investigation, the Bay of Bintuni Polres Recrement Sat has used the investigation e-management application but has not been adequate. This study aims to find out the existence or absence the effect of e-management application from investigation and organizational culture on investigator performance in handling criminal matters. The study was a quantitative with a research sample of 76 people. Testing hypotheses use classical assumption tests and simple regression analysis. The result was first no significant influence between the application of the investigation e-management to the performance of investigators in criminal matter handlers. Second, there was a significant influence between organizational cultures on investigator performance in criminal matter handlers. And the last was a significant influence between the application of investigation e-management and organizational culture together on the performance of investigators in criminal cover handling.