Plants are among the richest sources of bioactive compounds throughout the world for thousands of years and continue to provide new remedies to mankind. Roots of Hemidesmus indicus R. Br. is an important plant drug which is used to cure leprosy, leucoderma, itching, skin disease, asthma, bronchitis, leucorrhoea, dysentery, piles, syphilis, paralysis, urinary disorders and diabetes mellitus. As per Acharya Charaka has mentioned suitable season for collection of Moola i.e. Greeshma and Shishira Rutu. Here an attempt to study to check the potency of root collected as per classical reference. The present study focused on the pharmacognostical, phytochemical investigation as well as HPLC study were performed by taking different solvent extracts of Hemidesmus indicus root. This study highlights the detailed HPLC study on Hemidesmus indicus root by taking different solvent extracts with their increasing polarity which is a referential information for identification parameters and improves our confidence level of acceptability of herbal drugs.