Classical Probe Research Articles

Invariant tensions from holography

We revisit the problem of defining an invariant notion of tension in gravity. For spacetimes whose asymptotics are those of a Defect CFT we propose two independent definitions: gravitational tension given by the one-point function of the dilatation current, and inertial tension, or stiffness, given by the norm of the displacement operator. We show that both reduce to the tension of the Nambu-Goto action in the limit of classical thin-brane probes. Subtle normalisations of the relevant Witten diagrams are fixed by the Weyl and diffeomorphism Ward identities of the boundary DCFT. The gravitational tension is not defined for domain walls, whereas stiffness is not defined for point particles. When they both exist these two tensions are in general different, but the examples of line and surface BPS defects in d = 4 show that superconformal invariance can identify them.

Flight Testing Of A Single Point Frequency Sweeping Filtered Rayleigh Scattering Probe

"We present a successful in-flight application of filtered Rayleigh scattering (FRS) for temperature measurement in close proximity to the air plane using a newly developed single point, frequency sweeping probe. The temperature measurement is of relevance in the quantification of the density required for the evaluation of velocity from Pitot-probe measurements to obtain the true air speed (TAS). Laser optical air data sensors have the potential for contactless measurements outside the aerodynamic boundary layer. They are capable of self-diagnosis of fault conditions (e.g. signal loss, increased measurement uncertainty or spectral distortion for FRS) thereby increasing the safety of the aircraft. Compared to classical probes, they are less susceptible potential icing and blockage issues. The FRS-results presented here, are the outcome of two separate flight campaigns, carried out in the Alpine region as well as in the North Sea area. Measurement data provided information on the functionality of the FRS-system under various flight and weather conditions. Furthermore, the implementation of the FRS measurement system in the aircraft is presented; application aspects and improvements are discussed. In particular, the measurement accuracy at the actually achievable measurement rate was analyzed. For straight ahead flights at constant height, temperature mean values were in good agreement with the flight data system of the DLR research aircraft Dassault Falcon 20 (D-CMET). Aiming at in-flight air data measurements, the optical FRS measurement system requires a real-time temperature sample rate of a few Hz. We demonstrate that 2 FRS spectra per second can be acquired by a newly developed method using frequency sweeps for temperature determination. The low signal intensity of cw-laser based FRS is countered using a combined transmitter/receiver optic that focusses the entire cw-laser power into one measuring point. The light backscattered from the probe volume is collected by the same optics, filtered and imaged in a confocal arrangement onto a photomultiplier. An improved and miniaturized version of the presented FRS system could be a part of a future optical air data system (OADS). However, the strength of FRS signal relative to bright ambient conditions (e.g. sunlight, cloud backscatter) must be further improved in order to be able to measure well above cloud cover. The potential for further development is foreseen even if the accuracy required for flight data systems (~0.5 K) has not yet been achieved at a temperature measurement rate of 2 Hz. "

100-km entanglement distribution with coexisting quantum and classical signals in a single fiber

The development of prototype metropolitan-scale quantum networks is underway and entails transmitting quantum information via single photons through deployed optical fibers spanning several tens of kilometers. The major challenges in building metropolitan-scale quantum networks are compensation for polarization fluctuation, high-precision clock synchronization, and compensation for cumulative transmission time fluctuations. One approach addressing these challenges is to copropagate classical probe signals in the same fiber as the quantum signal. Thus, both signals experience the same conditions, and the changes of the fiber can therefore be monitored and compensated. Here, we demonstrate the distribution of polarization-entangled quantum signals copropagating with the White Rabbit precision time protocol classical signals in the same single-core fiber strand at metropolitan-scale distances. Our results demonstrate the feasibility of this quantum-classical coexistence by achieving high-fidelity entanglement distribution between nodes separated by 100 km of optical fiber. This advancement is a significant step towards the practical implementation of robust and efficient metropolitan-scale quantum networks.

Simultaneous electromagnetic field probing system with Y‐shaped separation detection structure

AbstractSimultaneous electromagnetic field probing system (SEMPS) has been popular in recent years. Herein, a simultaneous electromagnetic field probing system with Y‐shaped separation detection structure (SEMPS Y) is first proposed, in which the electric field probing region and the magnetic field probing region of the probe are separated by completely covering the shield along the loop and adding a pin‐shaped metal wire to capture the electric field, which are not the same as the classical dual probe structure. Combined with the non‐rotating asymmetric calibration method (NRACM), a 4‐port vector network analyser (VNA) and a highly symmetric grounded coplanar waveguide (GCPW) calibrator are used to solve the calibration matrix of the asymmetric SEMPS Y. The high symmetry GCPW calibrator is used to generate the standard electromagnetic field for calibration. The results of standing wave measurements show that the SEMPS Y can achieve ultrawideband electromagnetic field measurement of up to 20 GHz. Based on near‐field scanning measurements, SEMP Y can obtain results consistent with the calculation. In addition, when the probe is rotated 90° to invalidate the H‐field input, the decoupling curves of E‐field and H‐field are measured. Results show that the separation detection structure can effectively solve the cross‐coupling problem.

Photothermal spectroscopy on-chip sensor for the measurement of a PMMA film using a silicon nitride micro-ring resonator and an external cavity quantum cascade laser

Abstract Laser-based mid-infrared (mid-IR) photothermal spectroscopy (PTS) represents a selective, fast, and sensitive analytical technique. Recent developments in laser design permits the coverage of wider spectral regions in combination with higher power, enabling for qualitative reconstruction of broadband absorption features, typical of liquid or solid samples. In this work, we use an external cavity quantum cascade laser (EC-QCL) that emits in pulsed mode in the region between 5.7 and 6.4 µm (1770–1560 cm−1), to measure the absorption spectrum of a thin film of polymethyl methacrylate (PMMA) spin-coated on top of a silicon nitride (Si3N4) micro-ring resonator (MRR). Being the PTS signal inversely proportional to the volume of interaction, in the classical probe–pump dual beam detection scheme, we exploit a Si3N4 transducer coated with PMMA, as a proof-of-principle for an on-chip photothermal sensor. By tuning the probe laser at the inflection point of one resonance, aiming for highest sensitivity, we align the mid-IR beam on top of the ring’s area, in a transversal configuration. To maximize the amplitude of the photoinduced thermal change, we focus the mid-IR light on top of the ring using a Cassegrain reflector enabling for an optimal match between ring size and beam waist of the excitation source. We briefly describe the transducer design and fabrication process, present the experimental setup, and perform an analysis for optimal operational parameters. We comment on the obtained results showing that PTS allows for miniaturized robust sensors opening the path for on-line/in-line monitoring in several industrial processes.

Broadband Eddy Current Measurement of the Sheet Resistance of GaN Semiconductors.

Although the classical four-point probe method usually provides adequate results, it is in many cases inappropriate for the measurement of thin sheet resistance, especially in the case of a buried conductive layer or if the surface contacts are oxidized/degraded. The surface concentration of dislocation defects in GaN samples is known to challenge this kind of measurement. For the GaN sample presented in this study, it even totally impaired the ability of this method to even provide results without a prior deposition of gold metallic contact pads. In this paper, we demonstrate the benefits of using a new broadband multifrequency noncontact eddy current method to accurately measure the sheet resistance of a complicated-to-measure epitaxy-grown GaN-doped sample. The benefits of the eddy current method compared to the traditional four-point method are demonstrated. The multilayer-doped GaN sample is perfectly evaluated, which will allow further development applications in this field. The point spread function of the probe used for this noncontact method was also evaluated using a 3D finite element model using CST-Studio Suite simulation software 2020 and experimental measurements.

Northern blotting of endogenous full-length human-specific LINE-1 RNA.

LINE-1 belongs to a family of DNA elements that move to new locations in the genome in a process called "retrotransposition." This is achieved by a copy-and-paste mechanism with the aid of an RNA intermediate. The full-length LINE-1 is responsible for most retrotransposition activity in the human genome. Detecting the active LINE-1 RNA at the endogenous level is challenging due to its small percentage among inactive copies and its different forms of transcripts. Here, we describe a method of designing RNA probes to detect active LINE-1 by northern blotting and use optimized conditions and tools to make the detection practical. This method uses a classical long RNA probe and provides an alternative way to detect LINE-1 RNA using multiple short RNA probes.

Plasma density distribution and its perturbation by probes in axially symmetrical plasma

An analysis of plasma density distributions at arbitrary ion–atom collisionality for one-dimensional axially symmetrical cylindrical and annular plasmas is presented. Perturbations of plasma densities caused by a cylindrical probe are studied for arbitrary ion–atom collisionality. Analytical expressions for the plasma characteristics near the probe for low collisionality have been obtained. The plasma was modeled by the hydrodynamic neutral plasma equations, taking into account ionization, ion inertia, and a non-linear ion frictional force, which dominates the plasma transport at low gas pressures. Significant plasma density depletion around the probe has been observed for a wide range of ion–atom collisionality. The presented results predict underestimation of plasma density obtained from the classical Langmuir probe procedure and should provide a better understanding of electrostatic, magnetic, and microwave probes inserted into plasmas at low gas pressure.

Aggregation of gold nanoclusters in amyloid fibers: a luminescence assay for amyloid fibrillation detection and inhibitor screening.

Amyloid fibrillation is associated with a great variety of human diseases, such as Alzheimer's and Huntington's diseases. A fluorescence assay for amyloid fibrillation detection and inhibitor screening was developed based on the fact that the fluorescence emission of gold nanoclusters (Au NCs) is largely enhanced upon adding amyloids, such as lysozyme amyloid fibers. A good linear relationship exists between the enhanced fluorescence intensity of Au NCs and lysozyme fiber within the concentration range of 0-0.05 mg mL-1. This ultra-sensitive method can detect the protein fiber earlier than thioflavin T (THT), allowing more time for disease treatment. Furthermore, Au NCs have many advantages over the classical probe (i.e., THT), such as large Stokes shifts and low toxicity. We selected ascorbic acid as a representative inhibitor and used this method to screen inhibitors. If inhibitors are added when incubating lysozyme, the lysozyme fibrosis process will be crimped, decreasing the amount of lysozyme fibers.

Coding Intrinsic Disorder into DNA Hybridization Probes Enables Discrimination of Single Nucleotide Variants over Wide and Tunable Temperature Ranges

AbstractDNA hybridization probes are commonly used tools to discriminate clinically important single nucleotide variants (SNVs) but often work at elevated temperatures with very narrow temperature intervals (ΔT). Herein, we investigated the thermodynamic basis of the narrow ΔT both in silico and experimentally. Our study revealed that the high entropy penalty of classic hybridization probe designs was the key attributor for the narrow ΔT. Guided by this finding, we further introduced an entropy‐compensate probe (Sprobe) design by coding intrinsic disorder into a stem‐loop hybridization probe. Sprobe expanded ΔT from less than 10 °C to over 30 °C. Moreover, both ΔT and the optimal reaction temperature can be fine‐tuned by simply altering the length of the loop domain. Sprobe was clinically validated by analyzing EGFR L858R mutation in 36 pairs of clinical tumor tissue samples collected from lung cancer patients, which revealed 100 % clinical sensitivity and specificity. We anticipate that our study will serve as a general guide for designing thermal robust hybridization probes for clinical diagnostics.

Coding Intrinsic Disorder into DNA Hybridization Probes Enables Discrimination of Single Nucleotide Variants over Wide and Tunable Temperature Ranges.

DNA hybridization probes are commonly used tools to discriminate clinically important single nucleotide variants (SNVs) but often work at elevated temperatures with very narrow temperature intervals (ΔT). Herein, we investigated the thermodynamic basis of the narrow ΔT both in silico and experimentally. Our study revealed that the high entropy penalty of classic hybridization probe designs was the key attributor for the narrow ΔT. Guided by this finding, we further introduced an entropy-compensate probe (Sprobe) design by coding intrinsic disorder into a stem-loop hybridization probe. Sprobe expanded ΔT from less than 10 °C to over 30 °C. Moreover, both ΔT and the optimal reaction temperature can be fine-tuned by simply altering the length of the loop domain. Sprobe was clinically validated by analyzing EGFR L858R mutation in 36 pairs of clinical tumor tissue samples collected from lung cancer patients, which revealed 100 % clinical sensitivity and specificity. We anticipate that our study will serve as a general guide for designing thermal robust hybridization probes for clinical diagnostics.

Hormone effects of eighteen bisphenol analogues and their effects on cellular homeostasis and the typical signal pathways

Through the transfer chain of surroundings from feed to the farmed-animals and ultimately the corresponding livestock and poultry products, people are exposed to large amounts of bisphenol analogues (BPs), such as rational emissions from manufacturing plants, feed packaging bags and food packaging contact. Some BPs have been reported to show certain toxicological effects, especially, estrogen and endocrine disrupting effect. With the increasing application of BPs, the problem is becoming more and more serious. We systematically studied the hormonal effects of 18 BPs and their effects on cell homeostasis and classical signaling pathways by using classical E-SCREEN assay, fluorescent probes and western blotting. The results confirmed the estrogen-like effect of 13 BPs and 6 BPs obtained high docking scores (Scores < −9.0) for the three receptors simultaneously with the main interactions of hydrophobic, hydrogen and π-stacking of T-type bonds. BPAP regulates cells via apoptosis and steroid signaling pathway by intracellular ROS and mitochondrial followed the caspase pathway. BPE and BPS were involved in the classical NF-κB and Hippo signaling pathways. All data provides scientific basis for the safety risk assessment of endocrine disrupting and cellular homeostasis evaluation of BPs as chronic environmental pollution.

Second sound resonators and tweezers as vorticity or velocity probes: Fabrication, model, and method.

An analytical model of open-cavity second sound resonators is presented and validated against simulations and experiments in superfluid helium using a new resonator design that achieves unprecedented resolution. The model incorporates diffraction, geometrical misalignments, and flow through the cavity and is validated using cavities operated up to their 20th resonance in superfluid helium. An important finding is that resonators can be optimized to selectively sense either the quantum vortex density carried by the throughflow-as typically done in the literature-or the mean velocity of the throughflow. We propose two velocity probing methods: one that takes advantage of misalignments between the tweezers' plates and other that drives the resonator non-linearly, beyond a threshold that results in the self-sustainment of a vortex tangle within the cavity. A new mathematical treatment of the resonant signal is proposed to adequately filter out parasitic signals, such as temperature and pressure drift, and accurately separate the quantum vorticity signal. This elliptic method consists in a geometrical projection of the resonance in the inverse complex plane. Its effectiveness is demonstrated over a wide range of operating conditions. The resonator model and elliptic method are being utilized to characterize a new design of resonators with high resolution, thanks to miniaturization and design optimization. These second-sound tweezers are capable of providing time-space resolved information similar to classical local probes in turbulence, down to sub-millimeter and sub-millisecond scales. The principle, design, and microfabrication of second sound tweezers are being presented, along with their potential for exploring quantum turbulence.

Emulating Quantum Entangled Biphoton Spectroscopy Using Classical Light Pulses.

We show that for a class of quantum light spectroscopy (QLS) experiments using n = 0, 1, 2, ··· classical light pulses and an entangled photon pair (a biphoton state) where one photon acts as a reference without interacting with the matter sample, identical signals can be obtained by replacing the biphotons with classical-like coherent states of light, where these are defined explicitly in terms of the parameters of the biphoton states. An input-output formulation of quantum nonlinear spectroscopy is used to prove this equivalence. We demonstrate the equivalence numerically by comparing a classical pump-quantum probe experiment with the corresponding classical pump-classical probe experiment. This analysis shows that understanding the equivalence between entangled biphoton probes and carefully designed classical-like coherent state probes leads to quantum-inspired classical experiments that yield equivalent signals and provides insights for the future design of QLS experiments that could provide a true quantum advantage.

Kinematic tests on a docking mechanism for microsatellites

DOCKS is a smart docking system developed within the framework of the Space Rider Observer Cube mission (SROC) but suitable for use in more general scenarios. This paper presents the mechanical design of the system and the kinematic tests performed on it. The system merges a classical probe drogue configuration with a gripper-like design, to manage the connection between the parts, and it is equipped with a suite of sensors to estimate the relative pose of the target. The system is also equipped with a dedicated computer, making it a smart standalone system. A series of kinematic tests have been conducted to validate the capability of the system to passively manage misalignment during the docking manoeuvre. The final tests are presented to assess the maximum load that the system could handle, even when inactive.

Synthesis and solvatochromism of dipodal and tripodal dyes derived from Brooker’s merocyanine

Brooker’s merocyanine (BM) is a classic solvatochromic probe, which exhibits a band in the visible region sensitive to changes in the polarity of the medium. BM shows a reversion in its solvatochromism, which has inspired much research on studies involving different families of dyes. We synthesized four novel dipodal and tripodal solvatochromic dyes (1b–4b) inspired by BM molecular structure. Characterization of the compounds in their protonated form used spectrometric and differential scanning calorimetry techniques. Dyes 1b–4b have an electron–donating phenolate moiety connected through a CHCH conjugated bridge to two or three N–methylpyridinium electron–acceptor groups. The probes exhibit negative solvatochromism in the solvents studied, showing very different colors with the change in the polarity of the solvent. Multiparameter strategies of Kamlet–Abboud–Taft (KAT), Catalán, and a modified KAT fitted the experimental data. The HBD acidity of the solvent represented the main contribution to the solvation of the probes. Performing electronic–structure calculations of the compounds at LC–BLYP/ma–def2–SVP/D4 level and employing water and DMSO as implicit solvents (SMD model) using software ORCA5.0 revealed that the longest–wavelength absorption bands in the dyes are due to a π → π* transition, from the phenolate (electron–donating) group to the N–methylpyridinium (electron–acceptor) groups. The calculations did not reproduce the experimentally observed negative solvatochromism of the dyes in the solvents studied. However, comparing the theoretical and experimental data of the present study with data from the literature provided important information regarding the results obtained.

Engineering a Red Fluorescent Protein Chromophore for Visualization of RNA G-Quadruplexes.

Synthetic red fluorescent protein (RFP) chromophores have emerged as valuable tools for biological imaging and therapeutic applications, but their application in the visualization of endogenous RNA G-quadruplexes (G4s) in living cells has been rarely reported so far. Here, by integrating the group of the excellent G4 dye ThT, we modulate RFP chromophores to create a novel fluorescent probe DEBIT with red emission. DEBIT selectively recognizes the G4 structure with the advantage of strong binding affinity, high selectivity, and excellent photostability. Using DEBIT as a fluorescent indicator, the real-time monitoring of RNA G4 in biological systems can be achieved. In summary, our work expands the application of synthetic RFP chromophores and provides an essential dye category to the classical G4 probes.

Aminoquinolines: Fluorescent sensors to DNA – A minor groove probe. Experimental and in silico studies

An aminoquinoline (AQ4) was developed and proven to be a new and efficient DNA minor groove fluorescent probe. The specificity for DNA minor groove was attested by comparing it with well-established DNA probes such as Hoechst stain, acridine orange, and ethidium bromide. AQ4 was similar to the Hoechst stain, a classic minor groove probe, and opposite to acridine orange and ethidium bromide, the typical intercalating probes. An advantage of AQ4 to the Hoechst stain was the higher fluorescent signal-to-noise ratio (+DNA/-DNA). The interaction with DNA leads to an exclusive fluorescent band centered at 590 nm. The red-shifted fluorescent band is associated with a new absorption band (490 nm), revealing a ground-state complex formation. The complexation was also evidenced by circular dichroism, anisotropy, and fluorescence lifetime. The complex AQ4-DNA was pH dependent, being favored in an acidic medium. This feature was related to the preferential interaction with a protonated form of AQ4, i.e., AQ4(H+), revealing the role of electrostatic forces, which was corroborated by the strong dependence on the ionic strength of the medium and, particularly, on magnesium ions. The complexes were studied by docking and molecular dynamics and confirmed the stability of AQ4(H+). Additionally, 500 ns simulations were performed by adding salts. The presence of the salts leads to the loss of the binding of AQ4(H+) after 52 ns (NaCl) and 142 ns (MgCl2). Experimental and in silico outcomes showed the advantages of the aminoquinoline over the commercial DNA minor groove stain, the Hoechst dye. Hence, we propose its further application in cell-based assays.

Fluorescent head-labelled phospholipid coumarin bioconjugate as a chemical reporter for antioxidant protection in liposomes

The oxygen radical absorbance capacity (ORAC) assay is considered a valuable analytical tool for measuring the protection afforded by an antioxidant compound to a target probe. Fluorescein is the classical probe used in the ORAC assays. In addition to not being a biological target for oxidation, fluorescein acts as a free molecule within the reaction media. Moreover, the probe specific location is the aqueous medium where its oxidation occurs. Hence, fluorescein is not ideal for inferring about the interactions of radicals with biomimetic membrane models within the liquid-lipid interface or deeper in the bilayer system.The aim of the present work was to explore the use of POPE-COUM probe, a 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamine labelled with 3-carboxycoumarin, to report the protection of antioxidants interacting with biomembranes using liposomes as a biomimetic model. The probe was obtained applying an improved bioconjugation by using continuous flow synthesis to afford the desired compound in just 5 min in 80% yield. The lipid peroxidation induced by 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) derived radicals was monitored directly by the fluorescence intensity decay of the POPE-COUM, without resorting to any other reporter, in large unilamellar vesicles (LUVs). The results obtained showed a delay on the oxidation of the probe compared to aqueous phase fluorescein which might indicate that POPE-COUM is not accessible to the attack by the AAPH-derived radicals and is protected towards the inside of the vesicle. Hence, the access to the embedded probe only happens when alteration of the LUV structure occurs. Fluorescence lifetime imaging microscopy studies support the fact that the fluorescent POPE-COUM probe is restricted to the lipidic layers, and no background fluorescence was observed.Ultimately, these outcomes represent the proof-of-concept for the use of POPE-COUM in ORAC assays and its suitability as biomarker of the oxidation state and integrity of cellular membrane biomimetic systems such as liposomes.

Higher-order statistics and intermittency of a two-fluid Hall–Vinen–Bekharevich–Khalatnikov quantum turbulent flow

The Hall–Vinen–Bekharevich–Khalatnikov (HVBK) model is widely used to numerically study quantum turbulence in superfluid helium. Based on the two-fluid model of Tisza and Landau, the HVBK model describes the normal (viscous) and superfluid (inviscid) components of the flow using two Navier–Stokes type of equations, coupled through a mutual friction force term. We derive transport equations for the third-order moments for each component of velocity involving the fourth-order moments, which are classical probes for internal intermittency at any scale, and revealing the probability of rare and strong fluctuations. Budget equations are assessed through direct numerical simulations of the HVBK flow. We simulate a forced homogeneous isotropic turbulent flow with Reynolds number of the normal fluid (based on Taylor's microscale) close to 100. Values from 0.1 to 10 are considered for the ratio between the normal and superfluid densities. For these flows, an inertial range is not discernible and the restricted scaling range approach is used to take into account the finite Reynolds number (FRN) effect. We analyse the importance of each term in budget equations and emphasize their role in energy exchange between normal and superfluid components. Some interesting features are observed: (i) transport and pressure-related terms are dominant, similarly to single-fluid turbulence; and (ii) the mathematical signature of the FRN effect is weak despite the low value of the Reynolds number. The flatness of the velocity derivatives is finally studied through the transport equations and their limit for very small scales, and it is shown to gradually increase for lower and lower temperatures, for both normal fluid and superfluid. This similarity highlights the strong locking of the two fluids. The flatness factors are also found in reasonable agreement with classical turbulence.