Aggregate Classes Research Articles

Hierarchical assembly and environmental enhancement of bacterial ice nucleators

Bacterial ice nucleating proteins (INPs) are exceptionally effective in promoting the kinetically hindered transition of water to ice. Their efficiency relies on the assembly of INPs into large functional aggregates, with the size of ice nucleation sites determining activity. Experimental freezing spectra have revealed two distinct, defined aggregate sizes, typically classified as class A and C ice nucleators (INs). Despite the importance of INPs and years of extensive research, the precise number of INPs forming the two aggregate classes, and their assembly mechanism have remained enigmatic. Here, we report that bacterial ice nucleation activity emerges from more than two prevailing aggregate species and identify the specific number of INPs responsible for distinct crystallization temperatures. We find that INP dimers constitute class C INs, tetramers class B INs, and hexamers and larger multimers are responsible for the most efficient class A activity. We propose a hierarchical assembly mechanism based on tyrosine interactions for dimers, and electrostatic interactions between INP dimers to produce larger aggregates. This assembly is membrane-assisted: Increasing the bacterial outer membrane fluidity decreases the population of the larger aggregates, while preserving the dimers. Inversely, Dulbecco's Phosphate-Buffered Saline buffer increases the population of multimeric class A and B aggregates 200-fold and endows the bacteria with enhanced stability toward repeated freeze-thaw cycles. Our analysis suggests that the enhancement results from the better alignment of dimers in the negatively charged outer membrane, due to screening of their electrostatic repulsion. This demonstrates significant enhancement of the most potent bacterial INs.

Decomposer faecal food web and C sequestration in soil. Can near infrared spectroscopy describe transfers and transformations from fresh organic inputs to protected forms in soil aggregates?

This study sought to characterize the transfer of agricultural organic inputs by macrofauna to the biogenic aggregate compartments of the soil and test the existence of a faecal food web process based on coprophagic feeding behaviours. In an experimental plantain crop field of Colombia, we applied the agroecological FBO (Fertilisation Bio Organique ®) technology, a nucleation technique which consists in planting perennial plants in 1.0 x 0.4 × 0.4 m deep trenches where low- and high-quality organic materials are added in a specific design, and endogeic earthworms are inoculated. We evaluated the effectiveness of Near Infrared Spectroscopy (NIRS) to track this flow of organic matter through different natural stages of decomposition represented by soil macroaggregates produced by soil macroinvertebrates as faecal pellets and casts. After one year, great part of the organic inputs had been either mineralized or incorporated into macroaggregates of different sizes and origins. Using NIRS and physicochemical laboratory analyses, we assessed the quality and quantity of organic matter in the different types of aggregates separated manually according to their origin: large biogenic aggregates (LBA), medium sized biogenic aggregates (MBA) and small biogenic aggregates (SBA); physical aggregates (PA), root aggregates (RA) and residual soil (RS).Earlier studies had shown that these structures were presumably formed by the activity of macroinvertebrates of three different functional groups, Diplopoda and Isopoda litter transformers for SBA, epidendogeic earthworms for MBA and mesohumic endogeic earthworms for LBA, organized in a feeding succession.A Coinertia analysis between showed significant covariation among the two sets of physico chemical (22 variables) and NIR spectral (100 different wavelength absorbances) characterization of 135 samples representative of the three classes of biogenic macroaggregates, physical aggregates and residual non macroaggregated soil, (RV = 0.55; p < 0.001). This analysis clearly separated biogenic structures and ranked them according to their size, from small SBA to medium sized MBA and large LBA. Physical PA aggregates and RS residual soil were projected close to the LBAs in the coinertia factorial plan. Multiple combinatorial data analysis CDA, associated 5 specific wavelength absorbance ranges with aggregate types and residual soil. Along the sequence from small to large biogenic aggregates, residual soil and physical aggregates, wavelengths associated to easily decomposed substrates (in ranges 1708–1716 nm; 1796–1948 and 2164–2316 nm) had progressively decreasing absorbances. Substrates associated to slowly decomposing aromatic, alkane and phenolic substrates either increased (1420–1436) or decreased (1284–1380) along this sequence.These results are compatible with the hypothesis of a progressive transformation and transfer of organic residues first into small biogenic aggregates that are presumably faecal pellets of Isopoda and Diplopoda, then to medium sized biogenic aggregates identified as casts of epiendogeic earthworms and finally to large biogenic aggregates expected to be the casts of the large endogeic earthworms present in these soils. A definitive verification of the hypothesis and the involvement of specific macroinvertebrates will require direct experimentation in controlled laboratory conditions.

Cover cropping enhanced soil aggregation and associated carbon and nitrogen storage in semi-arid silage cropping systems

Wind and water erosion in arid and semi-arid regions significantly degraded soil, reduced soil organic carbon (SOC) storage, and exacerbated the impact of climate change on agricultural productivity. Cover cropping is one of the approaches to improve soil health and reduce climate change impacts, yet its impacts on soil aggregation and associated SOC and nitrogen (N) storage in water-limited environments are poorly understood. This study aimed to evaluate soil aggregate dynamics and aggregate-associated SOC and soil total N in irrigated silage sorghum [Sorghum bicolor L. (Moench)] − corn (Zea mays L.) rotations with various cover crop mixtures: grasses + brassicas + legumes (GBL), grasses + brassicas (GB), grasses + legumes (GL), and no cover crops (NCC). Results showed in the second year of the study that, at 0–0.1 m depth, mean weight diameter and geometric mean diameter of dry aggregates were 22–23 % and 6 % greater, respectively, with cover crops than NCC. At the same depth, cover crop treatments had 15–17 %, 15–16 %, and 13 % greater SOC in 0.25–2, 0.053–0.25, and < 0.053 mm aggregate classes, respectively, compared to NCC. Similarly, at 0–0.1 m depth, N in < 0.053 mm fraction was 8–10 % greater with cover crops than without. In the fourth year of the study, water-stable aggregates (WSA) percent did not differ among treatments, but WSA-associated SOC was 31–37 % and 12–16 % greater with cover crops at 0–0.1 and 0.1–0.2 m depths, respectively, than without. The WSA-associated N at 0–0.1 m was 21–33 % greater with cover crops than without. Despite the inconsistencies in soil aggregate stability results, cover crop-integrated silage systems showed greater SOC and N within aggregate classes and water stable aggregates. This insight is crucial for advancing sustainability in silage production systems, particularly in the context of increasing climate change and variability in erosion-prone soils of arid and semi-arid regions.

Carbon in soil macroaggregates under coffee agroforestry systems: Modeling the effect of edaphic fauna and residue input

Crop diversification tends to favor the soil fauna community, soil aggregation, and consequently soil organic carbon (SOC) stock. Understanding the association between these attributes can help in understanding the dynamics of physical protection of soil organic matter. In this context, our study aimed to answer: (1) how does the edaphic macrofauna community and soil carbon and aggregate classes respond to two types of coffee agroforestry systems (coffee with Grevillea robusta and coffee with banana) and how these responses differ from native ecosystem; (2) how and to what extent are soil aggregation regulated by the complex structural interactions of plant residue input, SOC, and the soil faunal community? The work was conducted in the municipality of Planalto, state of Bahia, Brazil. Three systems were evaluated: agroforestry system of Coffee arabica L. with Grevillea robusta (CG); agroforestry system of Coffee arabica with Musa spp. (CB); and native forest (NF). Four plots were delimited in each system, in which dry fractionation of the soil was performed to obtain aggregates of classes >6, 6–4, 4–2 and < 2 mm. The macrofauna was sampled using the Tropical Soil Biology and Fertility Program method. The labile and total carbon of the soil and aggregates were determined and the carbon management indices were calculated. The CG and CB presented a greater amount of larger size aggregates (> 6, 6–4 and 4–2 mm) than the NF. The CB system provided more favorable conditions for the soil macrofauna. Despite this, both coffee agroforestry systems favored the occurrence of Oligochaeta. The CG was more favorable to maintain labile fractions of organic matter than the CB. The edaphic fauna show a close relationship with the formation of carbon aggregates and stabilization which was directly influenced by continuous input of plant residues in diverse coffee growing systems.

Mulching drive changes in soil microbial community assembly processes and networks across aggregate fractions

Soil microbial community assembly processes and networks in croplands have been widely explored; however, their dynamics and how they regulate winter wheat yield across distinct soil aggregate fractions under the combined effects of mulching and soil horizons have not been comprehensively understood. Therefore, based on a 9-y field experiment, the responses of soil bacterial and fungal community assembly processes and interkingdom association networks to mulching were specifically investigated at the soil aggregation level. Soil properties and microbial biomass were separated into distinct mulching in the topsoil (0–10 cm), and soil water content was considered the most critical factor. The soil bacterial community was affected mainly by mulching and soil horizon compared with the fungal community in microaggregates (<0.25 mm). Notably, the bacterial community displayed more robust stochastic processes than the fungal one, and microbial interkingdom association networks were more complex and stable in micro-than macroaggregates. Soil potential carbon mineralization, pH, and total nitrogen were the dominant properties regulating winter wheat grain yield in combination with microbial community composition, assembly processes, and networks in each soil aggregate class. Wheat yield decreased under straw mulching and was mainly regulated by bacterial community composition and assembly processes. Thus, this study enhanced our understanding of the regulations for wheat yield, which could facilitate soil microbial community management at the aggregation level for sustainable crop production in mulching conservation agroecosystems.

Microbial Community of Subsoiling Have a Stronger Ability to Utilize Carbon Sources Across Aggregate Classes in an Anniversary Rotation

Microbial Community of Subsoiling Have a Stronger Ability to Utilize Carbon Sources Across Aggregate Classes in an Anniversary Rotation

The role of agroecological systems in cation interaction and aggregate formation in drylands of northeastern Brazil

Agroecological production systems are used in dryland agroecosystems. Therefore, it is necessary to verify their contributions, especially with regard to the functionality of soil attributes. From this perspective, this study aimed to evaluate the influence of soil use and management on the structural, hydraulic, and chemical attributes of a Haplic Cambisol in the Apodi Plateau, RN, Brazil. The experimental design adopted was completely randomized, with treatments distributed in a 5×3 factorial arrangement corresponding to five sampling locations (fruit farming, horticulture, agroecological, intercropping of Zea mays and Vigna unguiculata (L.) Walp), and three soil layers (0.00-0.10, 0.10-0.20, and 0.20-0.30 m), in addition to the correlation matrix and principal component analysis as multivariate statistical techniques. The parameters evaluated included soil pH, nutrient levels (available P, K+, Ca2+, and Mg2+), total organic carbon, soil density, total porosity, field capacity, permanent wilting point, available water, aggregation, aggregate stability, weighted mean diameter of aggregates, and the content of organic matter, Ca2+, and Mg2+ in different soil aggregate classes. The study concluded that continuous soil use practices such as intercropping Z. mays and V. unguiculata and horticulture significantly reduce nutrient availability, organic carbon content, moisture, and soil aggregation. However, areas under agroecological management and fruit cultivation, which involve minimal soil disturbance, promote the improvement of these properties.

Derajat Kepadatan Lapis Pondasi Agregat Tanpa Penutup Aspal Menggunakan Metode Sand Cone pada Proyek Rekonstruksi Jalan Kecamatan Serai Serumpun, Kabupaten Tebo di Ruas Jalan Tanjung Aur Seberang-Malako Tinggi

Road infrastructure is very important for the economic progress of Tanjung Aur Seberang village residents. Economic development in Tanjung Aur Village, Tebo Regency has significantly increased traffic levels. If this is not balanced by improving the quality of transportation facilities and infrastructure, then this could cause serious problems. Determining the value of the degree of density of the foundation layer aggregate (soil) class S in obtaining soil stability with safe conditions in accordance with the requirements of density standards is the aim of this research. The methods used are direct tests in the field and in the laboratory. Testing soil density in the field using a sand cone tool follows the provision stated in SNI 03-2828-1992, while testing soil density in the laboratory follows the provisions stated in SNI 1742-2008. Soil density testing intervals vary between 50 meters, 75 meters, 100 meters, 150 meters, 200 meters depending on location conditions. The results sand cone testing was carried out on roads from STA 2+000 to 3+800. In the first test with 9 test points, the field density was obtained below 95%, namely STA 2+100 (94,141%), STA 2 +550 (94,389%) and STA 3 +800 (94,289). Theses results indicate that the required standard degree of field density has not been achieved, so that the base layer of class S aggregate can be added and compaction can be carried out again. And from STA 0 + 005 to 4 + 200 in the second test with 26 test points. Test results using a sand cone tool show that the average water content value is 7.513% with a density reaching 98.846%. and these results were in accordance with applicable standard requirements.

Investigation Of Strength Development In Conventional And High Strength Concrete Under Different Curing Conditions

The composite material obtained by blending cement, aggregate, water and, when necessary, an additive, placing a mixture with carefully adjusted proportions into molds of the desired shape and size without gaps and hardening under appropriate maintenance conditions, is called concrete or traditional concrete. In the 1960s, concretes with compressive strengths of 41 MPa and 52 MPa were used on the market in the USA. In the recent past, concretes with compressive strengths varying between 80 MPa and 100 MPa have been commercially applied in structures made with in-situ concrete and prestressed concrete structural elements. Strength developments of traditional and high-strength concretes under different curing conditions will be examined. For this purpose, central pressure tests were carried out on cube samples produced. For concrete production; For each aggregate class, cement, saturation water, mixed water and high strength concretes; It was prepared by weighing silica fume and superplasticizer. The prepared samples were stored in standard or cold water for 3 days, 7 days, 28 days and 90 days. In this study, the strength development of traditional and high-strength concretes under different curing conditions was examined experimentally. In accordance with the experimental work plan determined for this investigation, concrete cube samples were produced and these samples were subjected to the central pressure test. The concretes produced are in C55, C20 and C12 classes. As a result, although similar behaviors were observed in high-strength and conventional concretes cured in different environments, it was determined that the difference in curing temperature was more effective on the strength development than the inadequacy of curing in water. It should be noted here that it would be beneficial to conduct similar studies on different types of concrete.

Conversion of pure Chinese fir plantation to multi-layered mixed plantation increases organic phosphorus accumulation and transformation within soil aggregates

Evaluation of the impacts of environmental factors and microbial communities on soil organic phosphorus (Po) availability is needed to clarify P cycling and regulate plantations productivity. However, the effects on Po accumulation and transformation of converting pure Chinese fir plantation to multi-layered mixed plantation and their regulatory mechanisms remain unclear. The aim of this study was to examine the impacts of the transformation of pure stand of Chinese fir (Cunninghamia lanceolata; PP) to mixed plantation with multiple tree species (C. lanceolata, Castanopsis hystrix, and Michelia hedyosperma; MP) on Po accumulation and transformation within topsoil (0–10 cm) aggregates in subtropical China. Our results showed that the soil organic carbon (SOC), total nitrogen (TN), ammonium nitrogen (NH4+-N), and available phosphorus (AP), as well as the carbon‑nitrogen ratio of soil (C/Nsoil) and carbon‑phosphorus ratio of soil (C/Psoil) were significantly higher (P < 0.05) of all aggregates in MP than those in PP. The quantities of soil microbial biomass C, N, P (MBC, MBN, and MBP) in all different aggregates were significantly greater (P < 0.05) in MP than in PP. The phospholipid fatty acid contents related to bacteria, fungi, arbuscular mycorrhizal fungi (AMF), and actinomycetes were significantly greater in all aggregate classes (P < 0.05) in MP relative to PP. The activities of all tested C, N, and P hydrolytic enzymes, as well as labile organic P, moderately labile organic P, moderately resistant organic P, and highly resistant organic P contents, were significantly higher of most aggregate size classes in MP. Finally, redundancy analysis (RDA) suggested that Po fractions were primarily affected by the NH4+-N, litterfall biomass (LF), carbon‑nitrogen ratio of litter (C/Nlitter), SOC and fine root biomass (FR). Our findings suggest that converting pure Chinese fir plantations into multi-layered mixed plantations may represent an effective management strategy for promoting organic P accumulation and transformation by regulating soil and microbial properties in degraded soils of Chinese fir plantations.

Soil organic matter components and sesquioxides integrally regulate aggregate stability and size distribution under erosion and deposition conditions in southern China

Water erosion considerably affects the stability and particle size distribution of soil aggregates, but the underlying mechanisms of water erosion remain unclear. To this end, we selected four landscape positions (top-, up-, mid-, and toe-slope) with distinct erosion and deposition characteristics on a typical eroded slope in southern China to conduct experiments- aiming to investigate the main drivers of soil aggregate stability during erosion and deposition processes. Soil samples were collected from 12 sites, and 4 size classes (>2, 2–0.25, 0.25–0.053, and <0.053 mm) of soil aggregates were obtained using the wet sieving method. The composition and stability of the soil aggregates, as well as the contents of organic (organic matter components) and inorganic (iron-aluminum oxides) cementing materials of different particle sizes, were determined. The results indicated that erosion significantly reduced the aggregate stability and the >0.25 mm water-stable aggregate content (WR0.25) (P < 0.05). The mean weight diameter (MWD) and geometric mean diameter (GMD) values of the soil aggregates at the eroding site decreased, and the fractal dimension (FD) increased. Furthermore, erosion markedly reduced the humic acid (HA) and fulvic acid (FA) contents in the bulk soils and soil aggregates, while the HA content showed no obvious difference between the eroding and depositional sites. In addition to the presence of complexed iron/alumina oxides (Fep/Alp), erosion markedly reduced the contents of amorphous (Feo/Alo) and free-form (Fed/Ald) iron/alumina oxides in the bulk soils and size fractions. Moreover, Fed/Ald, Fep and Feo/Alo were present in the microaggregates, while Alp was found in the macroaggregates. Additionally, boosted regression tree (BRT) analysis indicated that FA (36.70 %), Feo (19.00 %), and Ald (12.71 %) were the crucial predictors of soil aggregate stability. These findings further confirm that the organic and inorganic cementing materials in red soils collectively contribute to aggregate stabilization under the impact of erosion. This study facilitates a deeper understanding of the mechanisms governing soil aggregate stability in eroded landscapes, and provides a theoretical basis for biogeochemical cycling processes.

Soil amendment and N fertilization strategies to improve C sequestration and storage in soil aggregates

AbstractManagement can affect soil quality through changes in carbon (C) stock, especially in protected C fractions of soil aggregates. Soil aggregation and C sequestration may be improved with soil correction (liming and gypsum) and nitrogen (N) fertilization, but it is not clear how these factors might interact to affect humic substances. The objective of the study was to evaluate the effects of calcium (Ca), magnesium (Mg), and N fertilizatilizer amendments on the accumulation of C, N, and humic substances in soil aggregates from an Oxisoil in Brazil. The production system was no‐till soybean [Gycine max (L.) Merr] double‐cropped with maize (Zea mays L.) and intercropped with forage grass since 2016. Treatments were no amendment, lime only, and lime + phosphogypsum, factorially arranged with and without annual N fertilization of maize. Soil was collected at 0–10, 10–20, 20–40, and 40–60 cm 6 years after initiation of the experiment. The combination of lime, gypsum, and N led to 13% greater total C stock and 20% greater total N stock within the 0–60 cm soil profile than the control. Levels of fulvic acid (FA) and humic acid (HA) were similar among aggregate classes, but humin was greatest in larger aggregates. Liming plus N decreased FA and HA, but gypsum application mitigated this negative effect. Application of limestone, gypsum, and N increased humin, mainly in the soil surface (0–10 cm). Soil C storage and stabilization was enhanced in large soil aggregates with the combined use of lime, gypsum, and N fertilization in humid tropical soils.

Influence of cover crops on soil aggregate stability, size distribution and related factors in a no-till field

Soil aggregation plays an essential role in maintaining soil structure. A three-year field experiment was undertaken to explore the effects of cover crops on soil aggregate stability indices (mean weight diameter, water-stable aggregates greater than 0.25 mm, and soil erodibility factor), size distribution and their correlations with nine soil physical and chemical properties (soil bulk density, pH, organic carbon, total nitrogen, C/N, calcium, phosphorus, potassium, and cation exchange capacity) at 0–5 and 5–10 cm depths in a Marietta silt loam soil in a no-till field, Northeastern Mississippi. The cover crop treatments included elbon rye (Secale cereale L.), daikon radish (Raphanus sativus ssp. acanthiformis), Austrian winter field peas (Lathyrus hirsutus), and their mixture. The samples were wet-sieved to five size classes of aggregates (> 2, 2–1, 1–0.5, 0.5–0.25, and < 0.25 mm). Results showed that the < 0.25 mm aggregates dominated (50.95–78.02 %) soil aggregate size distribution at 0–5 and 5–10 cm depths. The rye plot soil displayed the greatest values for mean weight diameter (0.58 mm) in the two soil depths. Soil water-stable aggregates greater than 0.25 mm value under peas was significantly higher by 68.61 % compared to the no cover crop treatment at 0–5 cm depth, while soil erodibility factor did not change significantly. Mean weight diameter, water-stable aggregates greater than 0.25 mm, soil erodibility factor, 1–0.5 mm aggregates, and < 0.25 mm aggregates were significantly correlated with soil organic carbon, pH, bulk density, phosphorus, and potassium. The dominant factors driving changes in three aggregate stability indices and size fractions (excluding 2–1 mm aggregates) were bulk density and pH. Furthermore, high pH restricted soil aggregate stability and size distribution. This study demonstrated the positive impact of cover crops, specifically peas and rye, on soil aggregate stability and size distribution. This study will provide valuable information for future research on soil stabilization in crop production management systems.

Long-Term Organic Cultivation in Greenhouses Enhances Vegetable Yield and Soil Carbon Accumulation through the Promotion of Soil Aggregation

The long-term use of fertilizers and pesticides in conventional cultivation has resulted in a decrease in soil productivity and vegetable yields in greenhouses. However, there is little research exploring the changes in soil organic carbon and the microbial community mediated by soil aggregates, or their impacts on soil productivity. This study investigated the properties of soil aggregates, including the levels of organic carbon fractions, microbial community, and enzyme activity with the three aggregate classes: microaggregates (&lt;0.25 mm), small macroaggregates (2–0.25 mm) and large macroaggregates (&gt;2 mm) under conventional cultivation (CC), integrated cultivation (IC), and organic cultivation (OC) in greenhouses. The results showed that (1) OC and IC promoted the formation of small macroaggregates and enhanced aggregate stability compared to CC; (2) SOC in the three size fractions of OC increased by 92.06–98.99% compared to CC; EOC increased by 98.47–117.59%; POC increased by 138.59–208.70%; MBC increased by 104.71–230.61%; and DOC increased by 21.93–40.90%, respectively; (3) organic cultivation significantly increased enzyme activity in all three particle-size aggregates and increased the relative abundance of bacteria in microaggregates as well as the relative abundance of fungi in small macroaggregates. Structural equation model (SEM) analysis revealed that organic farming practices fostered the development of smaller macroaggregates, elevated microbial and enzyme activities within soil aggregates, and facilitated the conversion of soil nutrients and carbon sequestration. Therefore, long-term organic cultivation increases soil carbon content and vegetable yield in greenhouses by increasing the proportion of small aggregates. In conclusion, long-term organic cultivation in greenhouses improves soil structure, increase soil fertility and vegetable yield, and has a positive impact on the environment. Organic cultivation increases soil fertility and contributes to maintaining ecological balance and protecting the environment in greenhouses.

Soil aggregate stability governs field greenhouse gas fluxes in agricultural soils

Agriculture is responsible for 30–50% of the yearly CO2, CH4, and N2O emissions. Soils have an important role in the production and consumption of these greenhouse gases (GHGs), with soil aggregates and the inhabiting microbes proposed to function as biogeochemical reactors, processing these gases. Here we studied, for the first time, the relationship between GHG fluxes and aggregate stability as determined via laser diffraction analysis (LDA) of agricultural soils, as well as the effect of sustainable agricultural management strategies thereon. Using the static chamber method, all soils were found to be sinks for CH4 and sources for CO2 and N2O. The application of organic amendments did not have a conclusive effect on soil GHG fluxes, but tilled soils emitted more CO2. LDA was a useful and improved method for assessing soil aggregate stability, as it allows for the determination of multiple classes of aggregates and their structural composition, thereby overcoming limitations of traditional wet sieving. Organic matter content was the main steering factor of aggregate stability. The presence of persistent stable aggregates and the disintegration coefficient of stable aggregates were improved in organic-amended and no-tilled soils. Predictive modelling showed that, especially in these soils, aggregate stability was a governing factor of GHG fluxes. Higher soil CH4 uptake rates were associated with higher aggregate stability, while CO2 and N2O emissions increased with higher aggregate stability. Altogether, it was shown that sustainable agricultural management strategies can be used to steer the soil's aggregate stability and, both consequently and outright, the soil GHG fluxes, thereby creating a potential to contribute to the mitigation of agricultural GHG emissions.

Radiation drives tertiary lymphoid structures to reshape TME for synergized antitumour immunity.

Radiotherapy (RT) plays a key role in the tumour microenvironment (TME), impacting the immune response via cellular and humoral immunity. RT can induce local immunity to modify the TME. It can stimulate dendritic cell maturation and T-cell infiltration. Moreover, B cells, macrophages and other immune cells may also be affected. Tertiary lymphoid structure (TLS) is a unique structure within the TME and a class of aggregates containing T cells, B cells and other immune cells. The maturation of TLS is determined by the presence of mature dendritic cells, the density of TLS is determined by the number of immune cells. TLS maturation and density both affect the antitumour immune response in the TME. This review summarized the recent research on the impact and the role of RT on TLS, including the changes of TLS components and formation conditions and the mechanism of how RT affects TLS and transforms the TME. RT may promote TLS maturation and density to modify the TME regarding enhanced antitumour immunity.

Carbon Mineralization in Soil Aggregate Classes Under Leguminous Tree Planting in North Fluminense, Brazil

Carbon Mineralization in Soil Aggregate Classes Under Leguminous Tree Planting in North Fluminense, Brazil

Optimal consumption for recursive preferences with local substitution — the case of certainty

We characterize optimal consumption policies in a recursive intertemporal utility framework with local substitution. We establish existence, uniqueness, and a version of the Kuhn–Tucker theorem. The structure of optimal consumption plans is described explicitly for a large class of aggregators. For Epstein-Zin preferences, the solution coincides with the solution of the corresponding time-additive preferences.

Distribution of Soil Organic Carbon Density Fractions in Aggregates as Influenced by Salts and Microbial Community

Understanding soil organic carbon (SOC) at the aggregate level is crucial for soil health in secondary-salinized greenhouse development. Nevertheless, the specific patterns and contributions of ion content and microbial communities on SOC density fractions at the aggregate level remain unclear in secondary-salinized soil. We investigated variations in salts [electrical conductivity (EC) and ions] and microbial communities across various aggregate classes in both a 16-year-use greenhouse and open-field soils. We also examined SOC density fractions, including the light fraction (LF), the heavy fraction of particulate organic matter (POM), and mineral-associated organic matter (MOM) across different aggregates. The findings revealed that a lower Ca2+/K+ along with elevated EC levels (average 2.49 mS cm−1) reduced the macroaggregate percentage in greenhouse compared to open-field conditions, with a lower EC of 0.58 mS cm−1. Bacterial diversity and community composition exhibited no variation across different aggregate sizes at both sites. Conversely, fungal diversity and relative abundance (primarily dominated by Ascomycota of 78.50%) substantially increased in microaggregates (&lt;0.25 mm) compared to macroaggregates (&gt;0.25 mm). Macroaggregates exhibited a higher proportion of LF and MOM (3.3–18.2%, 24.9–34.5%, and 2.9–4.0% for LF, MOM, and POM, respectively) than microaggregates. Correlation and redundancy analyses revealed that fungal diversity, particularly the relative abundance of Ascomycota in aggregates &lt; 0.25 mm, significantly and positively influenced (p &lt; 0.05) the proportion of MOM carbon in terms of the overall SOC (J-type). This study provides valuable insights into the distribution patterns of SOC within the secondary salt-affected soils.

High trophic level organisms and the complexity of soil micro-food webs at aggregate scale regulate carbon accumulation in cropland soils

Enhancing soil organic carbon (SOC) levels via improved fertilization strategies can promote soil health and support sustainable crop production. While bacteria and fungi play a key role in SOC accumulation, the effects of soil micro-food webs that include organisms from higher trophic levels remain uncertain. We sought to narrow this knowledge gap by clarifying the associations between SOC accumulation and soil micro-food webs at the aggregate scale under long-term (18 years) fertilization treatments, which included a no-fertilizer control (Ctrl), synthetic fertilizers (NPK), and partial synthetic nitrogen replacement with pig slurry (OMNPK) or crop straw (RSDNPK). The concentration of SOC was significantly increased by fertilization following the order RSDNPK (13.1 g kg−1) > OMNPK (11.4 g kg−1) > NPK (9.1 g kg−1) > Ctrl (6.4 g kg−1). Nematodes and protists accounted for approximately 20% of all nodes in the multitrophic networks. Fertilization increased network complexity at the aggregate scale by 1–4 times across different sampling dates (P < 0.05). In all aggregate classes, the functionally important (FI) nematodes, protists, and bacterial taxa involved in soil carbon cycle had higher relative abundances under the fertilization treatments than under the Ctrl (P < 0.05). Structural equation modeling further indicated that besides soil microbes, network complexity, FI nematodes and protists were important to SOC accumulation. Compared with NPK, the key moments during which these soil biological attributes strongly regulated SOC under OMNPK and RSDNPK were delayed to late growth stages of maize. In conclusion, the increased network complexity and relative abundance of functionally important organisms from higher trophic levels of the soil micro-food webs at certain periods are vital contributors to effective SOC accumulation under fertilization regimes that include organic amendments.