Cladonia Stellaris Research Articles

The Key Role of Cyclic Electron Flow in the Recovery of Photosynthesis in the Photobiont during Rehydration of the Lichen Cladonia stellaris

Lichens are poikilohydric organisms and an important part of the ecosystem. They show high desiccation tolerance, but the mechanism of dehydration resistance still needs to be studied. The photosynthesis recovery of the photobiont in rehydrated lichen Cladonia stellaris after 11-year desiccation was investigated by simultaneously monitoring both photosystem I and II (PSI and PSII) activities. The responses of the photochemical efficiency and relative electron transport rate (rETR) of PSI and PSII, and the quantum yield of the cyclic electron flow (CEF) were measured using a Dual-PAM-100 system. PSI recovered rapidly, but PSII hardly recovered in C. stellaris during rehydration. The maximal photochemical efficiency of PSII (Fv/Fm) was generally very low and reached about just 0.4 during the rehydration. These results indicated that PSII had restored little and was largely inactivated during rehydration. The quantum yield of PSI recovered quickly to almost 0.9 within 4 h and remained constant at nearly 1 thereafter. The results showed that the activation of the CEF in the early stages of rehydration helped the rapid recovery of PSI. The quantum yield of the CEF made up a considerable fraction of the quantum yield of PSI during rehydration. A regulated excess energy dissipation mechanism and non-photochemical quenching (NPQ) also recovered. However, the small extent of the recovery of the NPQ was not enough to dissipate the excess energy during rehydration, which may be responsible for the weak activity of PSII during rehydration. The results indicated that both CEF and NPQ were essential during the rehydration of the photobiont in C. stellaris. The methods used in the measurements of chlorophyll a fluorescence and P700+ absorbance changes in this study provided a speedy and simple way to detect the physiological characteristics of the photobionts of lichen during rehydration. This work improves our understanding of the mechanism behind lichen’s desiccation tolerance.

Photosynthesis of widespread lichen species in pine forests of Central Siberia

Lichens and other terrestrial photosynthetic unicellular organisms of the planet consume nearly 14.3 billion tons of atmospheric CO2. Due to climate change, such important components of the forest ground cover as lichens are very vulnerable. This study evaluates the photosynthetic activity in widespread lichens by measuring the indices of net photosynthesis, dark respiration, and prompt fluorescence. Hence, cryptogams of pine forests in Central Siberia near the Zotino tall tower observatory (ZOTTO) are characterized as highly active. Cladonia stellaris (Opiz.) Brodo and Cladonia rangiferina (L.) are the main representatives of ground cover species. The purpose of this study was to determine the photosynthetic activity in dominant species of ground cover lichens during a growing season. We found the seasonal dynamics of photosynthesis with the lowest values being observed in June, and the highest ones in August. Dark respiration peaks in June and is the lowest in September. Fluorescence values are within the range of 6.7 ± 0.3. The species under study that grow on podzol soils in pine forests show fast kinetic activation.

STOCKS OF POTENTIALLY TOXIC ELEMENTS IN THE GROUND COVER OF NORTHERN TAIGA PINE FORESTS UNDER AEROTECHNOGENIC POLLUTION

The paper presents the results of studying the distribution of stocks of potentially toxic elements (Ni, Cu) in subcrown spaces and “windows” of stands in medium-aged pine forests in the background area of the Kola Peninsula and that under environmental pollution by atmospheric emissions of the Severonickel Combine (Monchegorsk, Murmansk Region). On the basis of previously obtained data on the stocks of aboveground biomass of the components of the ground cover, mortmass of plant debris and forest litter and data of chemical analysis by means of atomic absorption spectrometry of leaves of dominant shrub species, aboveground parts of the moss Pleurozium schreberi (Brid.) Mitt, and lichen Cladonia stellaris (Opiz) Pouzar et Vězda, as well as plant waste and organogenic horizon (forest litter) of Al-Fe-humus podzols, Ni and Cu stocks in different components of forest ecosystems were calculated. It was found that the sharp decrease in atmospheric emissions by the Severonickel Combine over the last 20 years has not affected the level of habitat contamination, and the stock of heavy metals in the upper organogenic horizon of Al-Fe-humus podzols remains very high, especially in the impact zone, which does not even allow to start the process of restoration of the ground cover in this area. The stock of heavy metals in the plant waste consistently increases with the increase in the level of forest litter contamination when approaching the source of pollution, but the scale of this increase is significantly smaller in the mortmass compared to the litter. The pattern of distribution of heavy metal stocks in components of ground cover, plant waste and forest litter over the phytocenosis area is fundamentally different in background conditions and under aerotechnogenic pollution, which is due to differences in the accumulation of organic matter in different types of microsites. The ratio of Ni : Cu concentrations and stocks also differs in the soil-vegetation cover in pine forests of the background area and under the influence of aerotechnogenic pollution.

Epiphytic and epigeal lichens as bioindicators of air pollution in the Burabay National Park, Kazakhstan

Abstract. Bukabayeva Z, Abiyev S, Silybayeva B, Assanova U, Sharipkhanova A, Sagdatkyzy B. 2023. Epiphytic and epigeal lichens as bioindicators of air pollution in the Burabay National Park, Kazakhstan. Biodiversitas 24: 2701-2709. Lichens are recognized as a symbiotic association between a fungus (mycobiont) and a chlorophyll-containing partner (photobiont). Because lichens receive all their nutrients from the atmosphere, they are very sensitive to air quality and can be used as bioindicators of pollution by heavy metals, organic compounds and radioactive elements in the air. This study aimed to evaluate the potential of lichens as bioindicators of the heavy metal content in the air in the Burabay National Park, northern Kazakhstan. In the period from 2018 to 2022, we studied the floristic composition of lichens in the national park. To inform air pollution level, we determined the quantitative characteristics and projective cover of an epiphytic lichen Evernia prunastri (L.) Ach. at different heights above ground level, and the heavy-metal content in an epigeal lichen Cladonia alpestris (L.) Rabenh (Syn. Cladonia stellaris (Opiz) Pouzar & V?zda) at different distances from the road edge. We identified 56 species of lichen belonging to 23 genera and 16 families on the roadsides or in the nearby forest. We found that the average number of individuals of E. prunastri decreased as the tree trunk height increased. The largest number of individuals (11.3) was observed at a height of 60 cm, and the smallest (2.5), at a height of 150 cm. The analysis of C. alpestris samples taken at different distances (50, 100, 150, 200 m) from the roadside showed that the concentrations of Pb, Cr, Cd, As, Ga, V, and ?s were high, and for all the elements studied, except for Mn and Be, exceeded Maximum Allowable Concentration. Our study demonstrates that E. prunastri and C. alpestris are sensitive to air pollution from road traffic and can be used as biomonitors of heavy metal pollution in the study area. Because of the ever-increasing anthropogenic pressure on the vegetation of the Burabay National Park, we recommend further research and monitoring of its lichen biota.

Comparative analysis of the effectiveness of bioindication methods in monitoring environmental studies in St Petersburg

The purpose of the research is to evaluate the effectiveness of bioindication methods in determining the state of the environment in St. Petersburg on the example of Vasileostrovsky and Pushkinsky districts. The content of chemical elements in soils, poplar bark Populus balsamifera, Tilia cordata and Betula pendula leaves, moss (Sphagnum angustifolium) and lichen (Cladonia stellaris) traps was studied, the integral index of fluctuating asymmetry of linden leaves T. cordata and birch B. pendula was calculated, soil biotesting using Daphnia magna and Chlorella vulgaris was carried out. It is established that the main sources of pollution of the urban environment are motor transport, the Baltic plant in Vasileostrovsky district and the industrial zones "Shushary" and "Pushkinskaya" in Pushkin district. The territory of the park zone of the Pushkin district can be considered as a conditional background for environmental monitoring. The use of fluctuating asymmetry of tree species leaves in bioindication studies is recommended only in conjunction with the results of chemical analysis. Poplar bark seems to be the most convenient bioindicator for assessing environmental pollution over a long period, the chemical composition of the leaves of tree species (linden T. cordata and birch B. pendula) reflects information about the intake of pollutants during one growing season, analysis of the chemical composition of moss and lichen traps allows you to determine the intake of pollutants in a relatively short period of time and identify sources of pollution. To assess the ecological state of the urban environment on the basis of the Harrington desirability function, an integral bioindication parameter BIP has been developed, taking into account the degree of transformation of individual environmental components under the influence of anthropogenic load: a change in the chemical composition of plants relative to clark (background) values; the manifestation of fluctuating asymmetry of tree species leaves compared to the norm. The effectiveness of BIP application for geoecological assessment of the state of the environment in cities is shown.

Estimating lichen diversity of Arunachal Pradesh, India along with new records for the country

Occurrence 686 species of lichen belonging to 185 genera and 52 families was earlier reported from the state of Arunachal Pradesh, India. In this study ninety four lichen species new to the state are added. This includes eight lichen species (Bunodophoron australe (Laurer) A, Massal, Cladonia stellaris (Opiz) Pouzar & Vězda, Graphis fujianensis Z.F. Jia & J.C. Wei, Graphis norstictica A.W. Archer & Lücking, Pertusaria subcopelandii Jariangpr, Pyrenula cruenta (Mont.) Vain., Ramboldia brunneocarpa Kantvilas & Elix and Usnea glabrata (Ach.) Vain.) which are new records for the country. The 780 lichen species belonging to 18 lichen bio-indicator communities. Graphidioid lichens are the dominant (18%) followed by Parmelioid (13%) and Pyrenulioid (09%) in Arunachal Pradesh. The present study can serve as a baseline for long term monitoring studies using lichen flora of the state as indicator.

Use of lichen species for air pollution biomonitoring: Case of Dar-Chichou forest (Cap-Bon, North-East Tunisia)

In the present work, we investigated the bio-uptake of 4 MTE (Ni, Cd, Pb, Zn) in the thalli of some species of lichens near a road crossing the Dar-Chichou forest (NE- Tunisia). In the absence of previous studies on the lichenic heritage of this forest, the objectives of this work are. first of all to identify for the first time the lichen species present in the forest, to determine the impact of this road pollution on certain physiological parameters of these pioneer species, such as the chlorophyll a and b levels, the content of carotenoids, theperoxidation (H2O2) and the ipoperoxidation (MDA) and on the other hand, to show the bioindicatory and bioaccumulative importance of these lichens in front of certain trace metals (Pb, Cd, Zn and Ni). In this study, 23 lichen species with different thallus was detected. The amounts of MTE in thalli of several lichen species were determined using the flame atomic absorption spectrometry. Significant positive correlations between the production of H 2 O 2 and the MDA content and on the MTE content (Ni, Pb, Zn) indicating a defense against oxidative stress. The presence of Evernia prunastri , Xanthoria parietina , Physcia adscendens and Cladonia stellaris , made it possible to classify the air quality of the Dar-Chichou Forest. P. adscendens present the weakest reaction against oxidative stress (H 2 O 2 ) and seemed the most resistant compared to the two other species. This study was conducted as part of a comprehensive approach combining chemical and biological parameters to assess the effect of road traffic on air quality. It provides the first database in Tunisia referring to MTE contamination and their probable biological effects. This study fills a necessary gap in the literature. • A first listing of lichens in the Tunisian Cap-Bon has been established. • An eco-biological evaluation of the air quality of the Dar-Chichou forest is established. • Impact of MTE on chlorophyll a and b , carotenoids, peroxidation (H2O2) and ipoperoxidation (MDA) levels has been done.

Co-dispersal of symbionts in the lichen Cladonia stellaris inferred from genomic data

We tested the congruence in dispersal patterns of the two main symbionts of the lichen Cladonia stellaris using genotyping-by-sequencing data. Based on 122 samples from eastern Canada, we recovered more than 21000 loci from the photobiont of C. stellaris . We described the population structure and estimate genetic diversity of the photobiont and identified the factors that contribute to explain genetic variation in both lichen partners. We also determined the identity of the dominant photobiont associated to C. stellaris using two molecular markers. Our results showed that C. stellaris is associated with Asterochloris glomerata , A. irregularis , and A. pseudoirregularis . Congruence in the genetic structure of photobiont and mycobiont were confirmed, suggesting co-dispersal of thallus fragments of C. stellaris . Genetic diversity of each symbiont was the factor that explained most of the genetic variation of the other symbiont, whereas geographical location and bioclimatic region seemed to have small or null explanatory power.

Establishing trace element concentrations for lichens and bryophytes in the ring of fire region of the Hudson Bay Lowlands, Ontario, Canada.

Peatlands dominate the landscape of the Hudson Bay Lowlands in Ontario, Canada. Recently, mineral deposits of chromium (Cr), nickel (Ni), and copper (Cu) were discovered in the region and anticipated future industrial mining operations have the potential to impact the environment. Lichens and bryophytes are considered excellent biomonitors and indicators of deposition, deriving their nutrients directly from the atmosphere. Trace element concentrations in lichens and bryophytes have not been reported in the Hudson Bay Lowlands. Here, we seek to determine the baseline trace element concentrations of six non-vascular species (Evernia mesomorpha, Bryoria spp., Cladonia stellaris, Cladonia stygia, Sphagnum fuscum, and Sphagnum capillifolium) common to the region, explore linear relationships of trace elements with iron (Fe) as a signature of particulates with geogenic origin, and calculate trace element enrichment factors. Thalli, foliage, and peat (0-30cm) were collected from 55 locations between 2013 and 2018 and analyzed for trace elements. Thalli and foliar concentrations are among the lowest reported in the broader literature and differ substantially from peat. Fe concentrations were significantly correlated (Pearson's r ≥ 0.8) with aluminum (Al), titanium (Ti), and vanadium (V) in all six species. Enrichment factors show some anthropogenic deposition effects non-vascular organism chemistry. Most trace element concentrations in lichens and bryophytes are indicative of long-range atmospheric transport of dust, but some is attributed to industry, with only minimal inclusions from the local area. Epiphytic lichens are well suited for ongoing atmospheric biomonitoring as industrialization commences.

Bacterial community of reindeer lichens differs between northern and southern lichen woodlands

Lichens cover nearly 7% of the earth’s surface, and in eastern Canada, lichen woodlands occupy over 300 000 km2. Reindeer lichens (genus Cladonia) are the main component of lichen woodlands and they play a crucial role in boreal forest ecology. We study, for the first time, the bacterial community of four species of reindeer lichens from eastern North America’s boreal forests. Using the 16S rRNA gene, we characterize the bacterial community of 189 lichen samples. We aim to analyse the effect of geography and host identity in the bacterial community composition and structure, verify the presence of a common core bacteria, and identify the most abundant core taxa. Our results suggest that host-lichen identity does not determine bacterial community composition and structure in reindeer lichens, but we confirm the influence of geography in shaping the diversity and abundance of bacteria associated with Cladonia stellaris. We also reveal that reindeer lichens share a reduced common core bacteria composed exclusively by Alphaproteobacteria. Northern lichen woodlands exhibit a significantly higher diversity and abundance of bacteria associated with C. stellaris than southern lichen woodlands do. The presence of the species Methylorosula polaris in the core bacteria is evident and may have a particular importance for reindeer lichens.

New locations of rare and endangered lichen species in the Zavolzhye region of the Chuvash Republic

The paper presents new data on rare and endangered lichen species found in the Trans-Volga Region of the Chuvash Republic from 2015 to 2020. Zavolzhye of the Chuvash Republic is the left-bank part of the republic, which is covered with coniferous, coniferous-broadleaved forests. According to the physical and geographical zoning of the Middle Volga region, Zavolzhye Chuvashia is included in the forest zone, province of southern taiga and mixed forests of the Low Volga region, in the mixed forest district, to the Vetluzhsko-Kokshagsky Polessky area, according to botanical and geographical zoning belongs to the Zavolzhsky Lowland-Polesky area of subtaiga forests, according to vegetative zoning to the Zavolzhsky Boreal area. As a result of field studies we found new habitats of 11 rare and endangered lichen species: Agonimia flabelliformis, Cetraria islandica, Cladonia stellaris, Mycobilimbia epixanthoides, Mycobilimbia carneoalbida, Platismatia glauca, Ramalina dilacerata, Usnea lapponica, Usnea dasopoga, Usnea subfloridana, Xanthomendoza ulophyllodes. For each species, the coordinates of the points of growth, distribution features in the republic, ecological and substratum and phytocoenotic confinement are indicated. The information on ecology and distribution of rare lichen species is clarified. Agonimia flabeliformis is a new and rare species for the Chuvash Republic

BIOGEOCHEMICAL EVALUATION OF THE VEGETATION STATUS OF REINDEER PASTURES OF THE NADYM DISTRICT OF THE YAMAL-NENETS AUTONOMOUS DISTRICT

The chemical elemental composition of the most widespread species of wood (Betula pubescens, Larix sibirica, Pinus sylvestris, Salix lanata), shrubs (Vaccinium vitis-idaea, Ledum palustust sl), herbs (Eriophorum angustifolium, Equisetum arvense) and lichens (Cladonia stellaris, Cladonia stygia). The concentrations of Cr, Co, Ni, Cu, Zn, Ga, As, Y, V, Na, Mg, Si, P, S, K, Ca, Ti, Mn, Fe, S obtained using the method of retgenofluorescence energy dispersion analysis.
 The features of the local biogeochemical background of plants are revealed and their geochemical specialization is studied. The greatest difference in the level of accumulation between different plant species was found for Ni, Zn, Ca, Mn, S, and Si. The analysis of the accumulation coefficients of chemical elements in plants relative to the local background level is carried out. Statistical significant differences in the elements accumulation by plants in different bioclimatic zones were revealed for Cu, Fe, Co, Cr, As, Mg, V, Y.
 The studied plants according to environmental safety criteria and the content of normalized micro- and macrocells mainly meet the requirements for fodder plants. The exception is the low content of nutrients Co, Na and K. For the prevention of animal diseases associated with a deficiency of essential elements, it is necessary to optimize the diet of deer by enriching feed with biologically active substances and macro- and microelements.

Distribution of 137Cs in lichens, mosses and pine needles along the transect from the north to the south of Western Siberia

The 137Cs content in mosses (Hylocomium splendens), lichens (Cladonia stellaris) and the needles of the Siberian pine (Pinus sibirica) and the common pine (Pinus sylvestris) along the transect from the north to the south of Western Siberia from N. 67.5° to N. 55° has been investigated. The appearance of 137Cs here is linked to the nuclear weapon tests at Novaya Zemlya. The measurements have shown that at all sampling points south of N. 59.6° there is a very sharp decrease in the 137Cs specific activity in all components of the ecosystem (in mosses and lichens by about 20 times, and in the needles of conifers by 100 times) instead of a smooth decrease. This fact can be explained by the existence of the global atmospheric circulation consisting of three circulation cells in the Northern hemisphere. It is just around N. 60° that the boundary between the Polar cell and the circulation cell of midle latitudes is drawn. At this boundary, the counter surface air flows of these cells (in our case, a contaminated flow from the north and a clean one from the south) collide and generate the upward air flows here that take 137Cs away. In addition, there is water vapor condensation in the upward flows resulting in snowfalls, rains and thunderstorms. And with these precipitation events, large amounts of 137Cs should have fallen out to the north of the Polar cell boundary during the Novaya Zemlya tests. The areas south around of N. 60° have been supposed to remain clean, which is what is being observed.

Разнообразие лиственничных лесов и редколесий западного макросклона Приполярного и Северного Урала

Разнообразие лиственничных лесов и редколесий западного макросклона Приполярного и Северного Урала

Разнообразие горных тундр с преобладанием в напочвенном покрове лишайников в пределах западного макросклона Приполярного и Северного Урала

В статье приведены данные о разнообразии сообществ горных тундр с преобладанием в напочвенном покрове лишайников ключевых участков Приполярного (хребты Малдынырд, Юаснырд, горы Баркова, Еркусей) и Северного (горный массив Тэлпозиз) Урала. Дана характеристика их структуры, видовой насыщенности, приведены списки доминирующего комплекса видов. Проведена NMS-ординация сообществ, позволившая выделить группы описаний, соответствующие синтаксонам, и показавшая высокую степень зависимости распределения выделенных групп от высоты расположения сообществ. Выявлено 12 синтаксонов лишайниковых тундр ранга ассоциации.

Растительность класса Loiseleurio procumbentis–Vaccinietea Eggler ex Schubert 1960 в восточноевропейских тундрах

Растительность класса Loiseleurio procumbentis–Vaccinietea Eggler ex Schubert 1960 в восточноевропейских тундрах

The potential antifeedant activity of lichen-forming fungal extracts against the invasive Spanish slug (Arion vulgaris)

The protection of horticultural crops from slug feeding can be achieved using slug pellets; however, application of molluscicides is not always safe for the environment. There is a need for alternative methods to reduce the palatability of crop plants. Chemical properties of secondary compounds from lichens influence the feeding behaviour of slugs. Liquid extracts of three lichen species (Cladonia rangiferina (L.) F.H. Wigg., Cladonia stellaris (Opiz) Pouzar & Vězda, and Pseudevernia furfuracea (L.) Zopf) were applied to three different crops and tested for their antifeedant properties against an important agricultural pest, the Spanish slug (Arion vulgaris Moquin-Tandon, 1855). All three extracts had specific activity, showing a decrease in grazing intensity as well as slug mass loss after feeding on treated food. Slugs significantly gained mass after feeding under control condition; however, they did not gain mass when fed on extract-treated food. The most effective extract was from P. furfuracea. We propose to use properties of lichen extracts to develop new environmentally friendly molluscicides.

Lichens facilitate seedling recruitment in alpine heath

AbstractQuestionsHow do mat thickness, physical structure and allelopathic properties of terricolous mat‐forming lichens affect recruitment of vascular plants in dwarf‐shrub and lichen heath vegetation?LocationThe mountains of Dovrefjell, central Norway.MethodsIn autumn, seeds of ten vascular plant species were collected and sown in a common garden experiment with mats of six lichen species and bare soil controls as experimental treatments. We recorded growing season soil temperature and moisture, and seedling recruitment and growth after one year. The effect of lichen secondary compounds on germination was tested in a growth chamber experiment and compared to the lichen–plant interactions detected under field conditions.ResultsThe lichen mats buffered extreme soil temperatures and soil drying in dry weather, with soils below the thickest mats (Cladonia stellaris and C. rangiferina) experiencing the lowest temperature fluctuations. Seedling recruitment and seedling growth in the field and seed germination in the lab were species‐specific. Seedling recruitment rates were overall higher within lichen mats than on bare soil, but the c. 6.5‐cm‐thick mats of C. stellaris reduced recruitment of many species. The lab experiment suggested no overall strong effect of lichen allelopathy on seed germination, and effects on seed germination were only moderately correlated with the lichen–plant interactions observed for seedling recruitment in the field.ConclusionsIn harsh environments like alpine dwarf‐shrub and lichen heaths, the presence of lichens and the resulting amelioration of the microclimate seem more important for vascular plant recruitment than are allelopathic effects often reported in lab experiments. We might therefore expect most terricolous lichens, depending on the plant species in focus, to facilitate rather than hamper the early stages of plant recruitment into lichen‐dominated arctic‐alpine heath vegetation.

Influence of tectonic faults on the conditions and properties of some components of a biogeocenosis in a subarctic area

In geoecology, the nature of the effect of tectonic faults on the environment is not well studied. The influence of natural landscapegeochemicalfactors on the state and properties of some components of biota of different hierarchical levels is studied at the intersection oftwo tectonic faults (Velsko-Ust’yanskiy tectonic knot (TK)) in the Arkhangelsk region. Two species of shrubby lichens (Cladonia stellaris Opiz.and Usnea subfloridana Stirt.) and woody plants (spruce – Picea abies L. and Scots pine – Pinus sylvestris L.) were chosen as test systems. Thefield studies were carried out at nine test sites (in the centre, on the periphery and some distance from TK – the background reference point)in different types of forest. The ash content of samples of the lichen Cladonia growing in the centre of the TK (1.12–1.22%) is double that in thecontrol area (0.56–0.58%), and for the lichen Usnea, it is seven times higher (6.82–6.99% at the centre and 0.97–1.09% in the control area). Theash content of tree bark collected at the centre of TK (1.27–1.29%) is double that at the control site (0.56–0.76%). This indicates a significantaccumulation of metals in the vegetation in the TK zone. The accumulation of heavy metals, the low water content of plants, the influenceof geomagnetic fields and other factors provoke excessive generation of active oxygen radicals and plants have various physiological,biochemical and morpho-biometric means of combating their adverse effects. The synergism of the cooperative protective action of lichenmatrix components on oxidative stress is expressed in terms of changes in biochemical parameters. At the centre of the TK, the lichenscontains up to 190 μg g−1 of ascorbic acid, whereas in the control area it does not exceed 130 μg g−1. The content of usninic acid in thecentre is 1.5–2 times higher for the Usnea subfloridana and is 1.5 times higher for Cladonia stellaris compared to the level in the control area.

Perception Evaluation Kit: A Case Study with Materials

This article aims to show the results from the Perception Evaluation Kit workshop: A Case Study with Materials. The study attempts to understand people’s sensorial perception of materials as a stimulus and what constitutes the most appropriate communication format for learning about their characteristics without losing information. Eleven people participated in this activity and, using evaluation tools in a test format, evaluated all the sensory properties of the following materials: Alusion, Cladonia stellaris, and Silkworms. These materials were presented in various interaction formats, called channels: text plus image–C1, video–C2, and physical sample plus audio–C3. Each participant evaluated one material per channel, without repeating samples or interaction formats. The activity was then discussed and, a month later, a question was asked to see what material they remembered best and how they described it. The data obtained were analysed with a two-way ANOVA statistical test to detect and compare the most notable differences. The most relevant results indicate a significant difference depending on the channel used to transmit the information of the materials and their sensory attributes, making it possible to evaluate the choice of the channel or the combination of channels to encourage more effective learning regarding materials