The aim of this study was to examine the diversity, relative abundance and ecology of mosquitoes in district Okara, Pakistan. A total of 2172 adult mosquitoes and 4317 mosquito larvae were collected, in which 13 mosquito species were identified within a single order, a single family and from three genera, Culex, Anopheles, and Aedes were identified. The identified 7 species belonged to the genus Culex, three species to the genus Anopheles and only 3 species belonged to the genus Aedes. The most abundant species was Culex quinquefasciatus with relative abundance of (17.5%), Culex theileri (15%), Culex duttoni (12.01%), Culex pipiens fatigans (10%), Anopheles subpictus (9.2%), and Anopheles annularis (8.13%). In contrast, Culex vagans, Culex sitiens, Anopheles stephensi, Culex tritaeniorhynchus, Aedes aegypti, Aedes albopictus, and Aedes vexans were identified to be the least in number with 5.61%, 5.52%, 4.41%, 4.1%, 3.8%, 3.02%, and 1.7% relative abundance. The Shannon-Weiner diversity index showed that Sardar Chok (H′=1.601) and Company Bagh (H′=1.458) had the greater diversity of mosquitoes, followed by Junior Model School, Green City, and Canal Road, i.e., 1.354, 1.345, and 0.6833. Species evenness has varied among Locations based on diversity, with values of 0.9918 (Sardar Chok), 0.9902 (Canal Road), 0.9679 (Junior Model School), 0.9592 (Green City), and 0.8596 (Company Bagh). The Simpson index (1-D) for Sardar Chok, Company Bagh, Junior Model School, Green City, and Canal Road was 0.7968, 0.7366, 0.7337, 0.7274, and 0.4902 indicating that SC and CB had the maximum Mosquitoes species. According to the results of this study, Okara district is home to a wide variety of mosquito species.
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