Citrus Pulp Research Articles

The Effects of Postharvest Treatments and Sunlight Exposure on the Reproductive Capability and Viability of Phyllosticta citricarpa in Citrus Black Spot Fruit Lesions.

Citrus black spot (CBS) is caused by Phyllosticta citricarpa, which is classified as a quarantine organism in certain countries whose concerns are that CBS-infected fruit may be a pathway for introduction of the pathogen. This study evaluated the reproductive capability and viability of P. citricarpa under simulated conditions in which the whole fruit, peel segments, or citrus pulp with CBS lesions were discarded. Naturally infected ‘Midknight’ Valencia orange and ‘Eureka’ lemon fruit, either treated using standard postharvest sanitation, fungicide, and wax coating treatments or untreated, were placed into cold storage for 5 weeks (oranges at 4 °C and lemons at 7 °C). Thereafter, treated and untreated fruit were incubated for a further 2 weeks at conditions conducive for CBS symptom expression and formation of pycnidia. The ability of pycnidia to secrete viable pycnidiospores after whole fruit and peel segments or peel pieces from citrus pulp were exposed to sunlight at warm temperatures (±28 °C) and ±75% relative humidity levels was then investigated. The combination of postharvest treatments and cold storage effectively controlled CBS latent infections (>83.6% control) and pycnidium formation (<1.4% of lesions formed pycnidia), and the wax coating completely inhibited pycnidiospore release in fruit and peel segments. Pycnidiospores were secreted only from lesions on untreated fruit and peel segments and at low levels (4.3–8.6%) from peel pieces from pulped treated fruit. However, spore release rapidly declined when exposed to sunlight conditions (1.4% and 0% after 2 and 3 days, respectively). The generally poor reproductive ability and viability of CBS fruit lesions on harvested fruit, particularly when exposed to sunlight conditions, supports the conclusion that citrus fruit without leaves is not an epidemiologically significant pathway for the entry, establishment, and spread of P. citricarpa.

Combined rotational and capillary rheomtery to determine slip coefficients and other rheological properties of orange pulp.

The characterization of the rheological properties of orange pulp under typical processing temperatures is needed for the design and optimization of orange pulp processing systems. The flow of orange pulp produced slip at shear rates at ∼1 to 5 s-1 . Rotational rheometry revealed that the flow behavior of orange pulp before slip occurrence followed the Power Law model for concentrations of ∼500 to 800 g/L at 4 to 80°C. The consistency coefficient (K) ranged from 33 to 234 Pa·sn and the flow behavior index n ranged from 0.18 to 0.24. Both, K and n decreased with temperature. While K fitted well an Arrhenius-like model, n best fitted a linear model. As concentration increased K increased linearly, while n was not significantly (P >0.05) affected. The flow without slip was calculated using the Power Law parameters from rotational rheometry and the wall shear stress (σw ) from capillary rheometry for the experimental flow rates. This allowed calculating the corrected slip coefficient βc and obviated the need for pipes with multiple diameters. βc decreased by one order of magnitude when temperature increased from 4 to 50 °C when σw was 0.1kPa. The effect was exacerbated with increased flow rate. Similarly, βc increased by about one order of magnitude when pulp concentration increased from ∼550 to 850 g/L at 80°C. The increase in βc with temperature indicated that the effect of temperature in the consistency of the bulk was different from its effect on the consistency of the liquid phase near the pipe wall. PRACTICAL APPLICATION: Design and optimization of processes equipment and industrial handling systems of orange pulp require detailed knowledge of their rheological (flow) properties. Citrus pulp like fruit pastes and purees produce less friction than one would anticipate when they flow because the liquid fraction acts as a lubricant. This study presents an original method for such characterization and shows that wall slip is greatly affected by temperature and concentration.

Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance

Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows. The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm. The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18). Processing scores of the WPCS were 32.1% (PT6), 53.9% (SP6), 49.0% (SP12), and 40.1% (SP18). Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation). Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis). Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch. Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18). Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6. Cows fed SP6 also produced more milk protein (+36 g/d), lactose (+61 g/d), and total solids (+94 g/d) than cows fed PT6. The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland. However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6. Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time. Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12. Compared with PT6, feeding SP6 raised linear odd-chain FA concentration in milk. Similarly, a reduction of these same FA occurred for SP12 compared with SP6. Cows fed SP6 had greater proportion of milk C14:1 and C16:1 compared with PT6 and SP12. Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen. Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.

Comparative effects of feeding citrus pulp and grape pomace on nutrient digestibility and utilization in steers

Feedlot beef production in tropics and subtropics is being limited by shortage of feed ingredients due to prolonged droughts resulting in high prices of cereal grains and reduced profitability. To alleviate feed shortages and increase profitability for the feedlot industry, it is important to explore underutilized non-conventional resources such as fruit by-products. The current study compared the nutrient intake, digestibility and utilization effects of feeding either 150 g/kg of dried citrus pulp (DCP) or grape pomace (DGP) as alternative dietary fibre sources to 7 months-old Angus steers (281 ± 15.7 kg) under feedlot conditions. Twenty-four steers were assigned to three dietary treatments (8 steers/treatment) in a completely randomised design. Each steer was treated as an experimental unit. Steers were adapted to diets for 21 d followed by 7 d of sample collection during which nutrient intake, digestibility, rumen fermentation parameters, nitrogen retention and microbial N supply were determined. All the data were analysed using the GLIMMIX procedures. Overall, steers fed DGP had the greatest dry matter intake (DM), organic matter (OM) crude protein (CP), ash free neutral detergent fibre (aNDFom), ether extract (EE) and starch intake followed by DCP and control (CON) diets (P ≤ .05). Apparent digestibilities of DM, OM and aNDFom were higher (P ≤ .05) for DCP diet compared to DGP and CON diets. Feeding DCP and DGP diets increased ruminal concentrations of total volatile fatty acids, acetate and isovalerate, and acetate to propionate ratio, and reduced propionate concentrations compared to the CON diet (P ≤ .05). The steers fed the CON diet had the greatest urinary excretions of allantoin, uric acid and total purine derivatives followed by those fed the DCP and DGP diets (P ≤ .05). Nitrogen intake, faecal nitrogen (N), N retention and N efficiency utilization were in the order of DGP > DCP > CON diets (P ≤ .05). Overall, feeding DGP as alternative fibre source to wheat bran improved nutrient intake, retention and efficiency of N utilization but reduced apparent nutrient digestibility compared to DCP. Current finding suggests that DGP may be a better fibre substitute for wheat bran in beef diets than DCP.

Polyunsaturated fatty acid, volatile and sensory profiles of beef from steers fed citrus pulp or grape pomace

The present study compared the effects of feeding dried grape pomace (DGP) or citrus pulp (DCP) at 150 g/kg dry matter compared to a control diet on major polyunsaturated fatty acids (PUFA), volatile and sensory profiles of beef. Feeding DGP or DCP diets to Angus steers for 90 d increased the proportions of C18:2n-6, C20:4n-6, C18:3n-3, total conjugated linoleic acid (CLA), n-3 and n-6 PUFA in muscle. Control-fed beef had greater concentrations of C18:1n-9, total aldehydes, ketones, and alcohols compared to DCP and DGP. Feeding DGP and DCP diets produced less tender beef than control. Overall, finishing steers on diets containing DGP or DCP compared to control increased proportions of total CLA, n-3 and n-6 PUFA, and reduced concentrations of aldehydes, ketones, and alcohols, but did not affect beef sensory attributes except for a slight reduction in tenderness.

Calcium salts of fatty acids with varying fatty acid profiles in diets of feedlot-finished Bos indicus bulls: impacts on intake, digestibility, performance, and carcass and meat characteristics.

We hypothesized that the inclusion of calcium salts of fatty acid (CSFA) into the diets and the fatty acid (FA) profile of the supplements would impact performance and meat characteristics of Bos indicus bulls. Hence, the objective was to evaluate the effects of CSFA profiles on intake, body weight (BW), carcass, and meat characteristics of feedlot-finished B indicus bulls. Fifty-three Nellore bulls [initial BW 315 ± 5.9 kg and 20 ± 2 mo] were used. At the beginning, 6 bulls were randomly chosen and slaughtered for determination of their BW composition, and the remaining 47 bulls were evaluated during a 140-d experimental period. The bulls were placed in individual pens, blocked according to initial BW and randomly allocated to 1 of the 3 following treatments: (1) control diet containing sugarcane bagasse, ground corn, citrus pulp, peanut meal, and mineral-vitamin mix (CON), (2) CON with the addition of 3.3% of CSFA from soybean oil (CSO), or (3) CON with the addition of a mixture of 3.3% of CSFA from palm, soybean, and cottonseed oils (CPSCO). Diets were offered ad libitum and formulated to be isonitrogenous. Bulls supplemented with CSFA had a greater (P < 0.01) final BW, dry matter intake, average daily gain (ADG), feed efficiency (FE), and FA intake vs. CON. Among carcass parameters, CSFA-supplemented bulls had greater (P < 0.01) carcass ether extract concentration vs. CON bulls. When the CSFA profile was evaluated (CSO vs. CPSCO), CPSCO bulls had a better (P ≤ 0.03) FE, carcass ADG, and hot carcass weight (HCW) vs. CSO bulls. The FA intakes differed among CSFA treatments, as the total saturated, palmitic, and oleic FA intakes were greater for CPSCO (P < 0.01), whereas lower intakes of total unsaturated and polyunsaturated FA (P < 0.01) were observed for CPSCO vs. CSO. Samples from the Longissimus muscle contained greater palmitoleic (P = 0.01) and reduced linoleic (P = 0.02) FA concentrations in CSFA-supplemented bulls vs. CON bulls. In agreement with the FA intakes, CPSCO-supplemented bulls had a greater (P ≤ 0.05) unsaturated FA concentration vs. CSO in Longissimus muscle. In summary, CSFA supplementation improved the performance of finishing B. indicus bulls vs. CON. Moreover, the inclusion of CSFA from palm, soybean, and cottonseed oil benefited the FE, carcass ADG, and HCW compared with the inclusion of CSFA from soybean oil, demonstrating the potential of specific FA for improving the performance and meat quality of B. indicus bulls.

Effect of Citrus By-product on Physicochemical Parameters, Sensory Analysis and Volatile Composition of Different Kinds of Cheese from Raw Goat Milk.

The increased use of concentrates to reduce pasture as a feed source in productive systems like Payoya breed goat farms has made it necessary to decrease feeding costs. The inclusion of agro-industry by-products such as dry orange pulp pellets in goat diets has been suggested as a sustainable alternative to cereal-based concentrates. The aim of this work was to assess the influence of diets including dry orange pulp pellets on the quality of cheeses traditionally made from Payoya breed goat milk. We analysed the physicochemical characteristics, sensory properties and volatile compound profiles of 18 artisanal cheeses made from raw Payoya milk. In this study, goats were fed with different concentrations of dry orange pulp; and cheeses were curdled with animal and vegetable coagulants. Slight differences were detected between some cheeses. However, the use of citrus by-products in the Payoya goat diets did not substantially affect the cheeses’ physicochemical properties, olfactory attributes, or volatile profiles. Therefore, dried citrus pulp can be used as a substitute for cereal concentrates without affecting the distinct properties of these ripened raw goat milk cheeses.

Feeding Agro-Industrial By-Products to Light Lambs: Influence on Meat Characteristics, Lipid Oxidation, and Fatty Acid Profile.

Simple SummaryConsumers demand safe and healthy animal products produced with minimal environmental impact. The use of agro-industrial by-products in animal feeding can alleviate pollution caused by their accumulation, but investigations into their effects on animal-product quality are required. We analyzed the influence of replacing 44% of conventional feeds in a high-cereal concentrate (CON) with agro-industrial by-products (BYP concentrate; distillers dried grains with solubles, dried citrus pulp, and exhausted olive cake) on meat quality of fattening light lambs. Two groups of lambs were fed each of the concentrates and barley straw ad libitum from 13.8 to about 26.0 kg of body weight. The pH, chemical composition, color, and texture parameters of the meat were not affected by the type of concentrate. Feeding the BYP concentrate significantly reduced lipid oxidation of meat after 6 days of refrigerated storage, which might be related to the greater content of polyphenols in this concentrate. Compared with CON-fed lambs, the meat from BYP-fed lambs had lower saturated and greater polyunsaturated fatty acid content. In summary, feeding the tested by-products did not change the composition of the meat but increased its shelf-life and improved its fatty acid profile.The aim of this study was to assess the effects of replacing 44% of conventional feeds in a high-cereal concentrate (CON) with by-products (BYP concentrate; 18% corn distillers dried grains with solubles, 18% dried citrus pulp, and 8% exhausted olive cake) on the meat characteristics and fatty acid (FA) profile of fattening light lambs. Two groups of 12 Lacaune lambs were fed concentrate and barley straw ad libitum from 13.8 to 26.0 kg of body weight. There were no differences (p ≥ 0.130) between groups in the pH, chemical composition, color, and texture parameters and in the estimated proportions of pigments in the longissimus dorsi. Feeding the BYP concentrate reduced the concentration of thiobarbituric acid reactive substances (TBARS) in the meat after 6 days of refrigerated storage (unmodified atmosphere), probably due to the greater polyphenol content in this concentrate. Compared with CON-fed lambs, the meat and the subcutaneous fat from BYP-fed lambs had lower saturated and greater polyunsaturated FA content as well as greater n-6/n-3 FA. In summary, feeding a blend of corn distiller dried grains with solubles, dried citrus pulp, and exhausted olive cake did not change the composition of the meat but improved its antioxidant status and FA profile.

Effect of Tomato Pomace, Citrus and Beet Pulp on Productive Performance and Milk Quality of Egyptian Buffaloes.

Using agro-industrial waste in animal diet became a new strategy in the animal feeding system to decrease the cost of nutrition. This study aimed to investigate the effects of usage tomato pomace, citrus and beet pulp in dried form in diets on the performance of lactating buffaloes and milk quality. Fifteen milking Egyptian buffaloes at the second and third seasons of lactation were divided into five groups (3 animals in each) to fed on five experimental rations. The experimental rations were: R1(control group) fed on Concentrate Feed Mixture (CFM1) contains 20% wheat bran+roughage, R2: CFM2 replacement wheat bran 10% Dried Tomato Pomace (DTP) and 10% Citrus Pulp Dried (CPD)+roughages, R3: fed CFM2 with 15 g fibrolytic enzyme/head/day+roughages, R4: Fed CFM3 replacement wheat bran 10% DTP and 10% Dried Beet Pulp (DBP)+roughages and R5: CFM3 with 15 g fibrolytic enzyme/head/day+roughages. Tested by-products observed different effects of nutrients digestibility and nutritive values comparing with control ration. There was no significant difference in fat corrected milk and milk fat among treatments. Polyunsaturated fatty acids especially C18:2c and C18:3n3 were recorded highly significant values with ration four. Rations 4 and 5 led to increased blood albumin, total protein. It could be concluded that tomato pomace, citrus and beet pulp could be used as alternative sources to replace wheat bran in buffalo's rations without adverse effects on milk yield with positive effects on milk quality and fatty acids profile.

Formulation of Orange Juice with Dietary Fibers Enhances Bioaccessibility of Orange Flavonoids in Juice but Limits Their Ability to Inhibit In Vitro Glucose Transport.

The effect of formulating orange juice (OJ) with dietary fibers (DFs) on in vitro bioaccessibility of flavonoids and their ability to inhibit glucose transport in Caco-2 cells were investigated on Valencia orange fruit (OF), OJ, and OJ formulated with 1 and 2.8% DFs. DFs were either orange pomace (P) or commercial pulverized citrus pulp fiber (CF). Juice extraction and formulation with CF led to minimal loss of flavonoids compared to formulation with P (474 μmol/100 g for OF vs 315-368 μmol/100 g for OJ and OJ with CF, and 266-280 μmol/100 g for OJ with P). Addition of DFs led to similar or improved flavonoid bioaccessibility compared to OJ (9.5% in OJ vs 7.9-33.4% with DFs) but higher glucose transport in Caco-2 cells (0.45 μmol/min in OJ alone vs 0.64-0.94 μmol/min with DFs). This paradoxical effect was attributed to potential complexation of flavonoids and DFs, preventing flavonoids from interfering with glucose transport.

Effects of Replacing Extruded Maize by Dried Citrus Pulp in a Mixed Diet on Ruminal Fermentation, Methane Production, and Microbial Populations in Rusitec Fermenters.

Simple SummaryCitrus pulp is the main by-product obtained from citrus processing. The high-moisture content of this by-product makes it rapidly perishable, its accumulation can cause environmental problems, and it causes high disposal costs for citrus processing factories. Therefore, alternative uses for citrus pulp are necessary, its use in ruminant feeding being one of the most feasible ones. In this study, we assessed the effects of replacing extruded maize in a diet for dairy sheep (20% of diet) by dried citrus pulp using an in vitro technique (Rusitec fermenters). Results showed some positive effects of citrus pulp on diet degradability and in vitro fermentation parameters. The growth of ruminal microbes and bacterial diversity were essentially unaffected. Our results indicate that maize in dairy sheep diets can be totally replaced by dried citrus pulp without negatively affecting ruminal fermentation. The use of citrus pulp would reduce the amount of human-edible ingredients used in the diet of dairy sheep.Citrus pulp is a highly abundant by-product of the citrus industry. The aim of this study was to assess the effects of replacing extruded maize (EM; 20% of total diet) by dried citrus pulp (DCP; 20%) in a mixed diet on rumen fermentation and microbial populations in Rusitec fermenters. The two diets contained 50% alfalfa hay and 50% concentrate, and the same protein level. Four Rusitec fermenters were used in a cross-over design with two 13-d incubation runs. After 7-d of diet adaptation, diet disappearance, fermentation parameters, microbial growth, and microbial populations were assessed. Fermenters receiving the DCP showed greater pH values and fiber disappearance (p < 0.001) and lower methane production (p = 0.03) than those fed EM. Replacing EM by DCP caused an increase in the proportions of propionate and butyrate (p < 0.001) and a decrease in acetate (p = 0.04). Microbial growth, bacterial diversity, and the quantity of bacteria and protozoa DNA were not affected by the diet, but the relative abundances of fungi and archaea were greater (p < 0.03) in solid and liquid phases of DCP fermenters, respectively. Results indicate that DCP can substitute EM, promoting a more efficient ruminal fermentation.

Fermentative parameters and aerobic stability of orange peel silage with pelleted citrus pulp

ABSTRACT This study evaluated the effects of pelleted citrus pulp (PCP) added to orange peel on fermentative parameters and aerobic stability of orange peel silages. The treatments were based on different levels of PCP: 0 (control), 10% PCP, 20% PCP, and 30% PCP calculated according to the weight of orange peel (w/w), with five experimental silos per treatment stored for 60 days. Chemical composition, fermentative parameters, microbial population, and dry matter (DM) losses were performed in silages after opening the [...]

Quantification of Morpholine in Peel and Pulp of Apples and Oranges by Gas Chromatography-Mass Spectrometry.

Morpholine salts of fatty acids have been used in wax coatings on the surfaces of fruit and vegetable commodities in China and the United States, etc. However, morpholine usage was prohibited in many other countries because of safety concerns. We optimized analytical methods to determine morpholine in the peel and pulp of fruits and vegetables by gas chromatography-mass spectrometry (GC-MS). This morpholine analysis method was applied to real samples of apples, citrus fruits, and vegetables from Korea, China, and the U.S. The method was validated using apple and citrus fruit peels and pulp. The method detection limit (MDL) was 1.3–3.3 µg/kg. The recovery rates of morpholine were 88.6–107.2% over a fortified level of 10–400 µg/kg. Intra-day and inter-day precisions were 1.4–9.4% and 1.5–2.8%, respectively. The morpholine concentrations were n.d. (not detected)–11.19 and n.d. (not detected)–12.82 µg/kg in apple and citrus peels, respectively. Morpholine was not detected in citrus or apple pulp samples or in vegetable samples.

The Use of Citrus Pulps As an Alternative Feed Sources in Rumınant Feeding and Its Methane Production Capacities

Hatay ilinde yetişen turunçgil posalarının ruminant beslemede alternatif yem kaynağı olarak kullanımı ve metan üretim kapasitesini belirlemek amacıyla; kuru madde (KM), pH, olması gereken pH (OlpH), fleig skoru (FS), ham kül (HK), ham protein (HP), ham yağ (HY), ADF, NDF, net gaz, net metan gazı, metabolik enerji (ME) ve organik madde sindirim derecesi (OMSD) içerikleri belirlenmiştir. Araştırma sonunda pH değerleri 2.72 ile 3.48 arasında bulunan turunçgil silajının düşük değerlere sahip olması nedeniyle uzun süre depolanabilir bir silaj türü olduğu söylenebilir. Turunçgil silajları metan gazı üretim kapasitesi açısından değerlendirildiğinde % 8.17 ile 11.23 arasında değerler almış olup; en düşük metan gazı içeriği kabuksuz greyfurt cinsinden en yüksek metan gazı içeriği kabuklu Washington cinsinden elde edilmiştir. Turunçgil silajlarının yonca, mısır gibi silaj türlerinin metan gazı içeriklerine (%16-18) göre daha düşük metan gazı üretim kapasitesine sahip olması nedeniyle atmosfer’e yayılan sera gazı etkisi bakımından düşük kapasiteli az zararlı bir silaj türü olarak değerlendirilebilir. Metan gazı üretim kapasitesi açısından kabuksuz turunçgil silajlarının kabuklu turunçgil silajlarına göre daha avantajlı olduğunu söylenebilir. Sonuç olarak meyve suyu üretim tesislerinde her yıl gelişi güzel etrafa dökülen binlerce ton turunçgil posaları yem maliyetini düşürmek, kötü koku oluşumunu ve çevre kirliğini önlemek amacıyla uygun şekilde katkı maddesi olmadan silolanıp hayvan beslemede kullanılabilir.

Feeding Microbiome-Targeting Ingredients Increases Fecal Plant-Origin Antioxidants and Anti-Inflammatory Compounds, and Decreases Branched-Chained Amino Acids in Cats

Abstract Objectives Inflammatory and oxidative processes play an important role in the maintenance of digestive health and progression of diseases. This study was performed to evaluate changes in fecal microbiota, metabolites, and post-biotics associated with increasing dietary concentrations of ingredients targeting the gut microbiota. Methods Fifty-six healthy adult cats were fed for four weeks on a complete and balanced adult cat food. After the four weeks, cats were randomly assigned to one of four treatment groups for the next 28-day feeding period: Control─continued feeding of the pretrial food; C + 1─fed a food similar to the control food with 1% enhanced fiber mix; C + 2─fed a food similar to the control food with 2% enhanced fiber mix; and C + 4─fed a food similar to the control food with 4% enhanced fiber mix. The fiber mix contained pecan shells, beet pulp, cranberry pomace, flaxseed, and citrus pulp. Fecal samples were collected after prefeeding, after 10 days on test foods, and at the end of the 28-day feeding period. Fecal samples were analyzed for: microbiota by 16SrRNA amplicon sequencing using V3-V4 hypervariable regions (performed in-house), short chain fatty acids and metabolomics analyzed by Metabolon, Durham, NC. Significance of P &amp;lt; 0.05 was used. Results There was an increase in the bacterium Faecalibacterium in cats eating C + 4% with no significant changes in the other treatment groups. There was a decrease in the abundance of branched chain fatty acids in the C + 4% group with no significant changes in the other treatment groups. The saccharolytic sugar arabinose was increased in both the C + 2% and C + 4% groups. There was an increase in many antioxidant and anti-inflammatory plant compounds and their respective post-biotics in cats fed test foods. Conclusions Increased arabinose concurrent with decreased branched chain fatty acids suggests a shift from proteolytic to saccharolytic metabolism by the GI microbiome. The transformation of fiber polyphenols into sugars, and derived polyphenols into compounds having increased antioxidant and anti-inflammatory potency, suggests that these ingredients nourish and activate the colonic microbiome to improve GI health. Funding Sources Hill's Pet Nutrition, Inc.

Feeding Microbiome-Targeting Ingredients Increases Fecal Butyrate, Plant-Origin Antioxidants, and Anti-Inflammatory Compounds in Dogs

Abstract Objectives Inflammatory and oxidative processes play an important role in the maintenance of digestive health and progression of diseases. This study was performed to evaluate changes in fecal microbiota, metabolites, and post-biotics associated with increasing dietary concentrations of ingredients targeting the gut microbiota. Methods Forty-eight healthy adult dogs were fed for four weeks on a complete and balanced adult dog food. After four weeks, dogs were randomly assigned to one of four treatment groups for the next 28-day feeding period: Control─continuation of the pretrial food; Control + 1─the control food with 1% enhanced fiber mix; Control + 2─the control food with 2% enhanced fiber mix; and Control + 4─the control food with 4% enhanced fiber mix. The fiber mix contained pecan shells, flaxseed, beet pulp, citrus pulp, and cranberry pomace. Fecal samples were collected after prefeeding, after 10 days on test foods, and at the end of the 28-day feeding period. Fecal samples were analyzed for: microbiota by 16SrRNA amplicon sequencing using V3-V4 hypervariable regions (performed in-house), short chain fatty acids (SCFA) and metabolomics (analyzed by Metabolon, Durham, NC. Statistical significance of P &amp;lt; 0.05 was used. Results There was an increase in the fecal SCFA butyrate with Control + 4%, but no significant SCFA changes in the other treatment groups. The saccharolytic sugar arabinose was increased at 10 days, but not at 28 days in the Control + 4% group. There was an increase in many antioxidant and anti-inflammatory dietary plant compounds and postbiotics (dosmetin, eriodictyol, limonin, naringenin, pheophoribide A, hesperidin, hesperetin, ponciretin, secoisolariciresionol diglucoside, secoisolariciresionol enterodiol) by 10 days and through the end of the feeding period. There were no significant changes detected in the microbiome. Conclusions Increased butyrate and arabinose suggest a shift from proteolytic to saccharolytic metabolism in the microbiomes of dogs fed the Control + 4% food. The transformation of fiber polyphenols into sugars, and derived polyphenols into compounds having increased antioxidant and anti-inflammatory potency, suggest that these ingredients nourish and activate the colonic microbiome to improve GI health. Funding Sources Hill's Pet Nutrition, Inc.

Quality indices and sensory attributes of beef from steers offered grass silage and a concentrate supplemented with dried citrus pulp

This study investigated the quality composition, oxidative stability and sensory attributes of beef (longissimus thoracis, LT) from steers offered grass silage and a concentrate supplement in which barley was replaced by 40% and 80% (as-fed basis) of dried citrus pulp (DCP). Dietary treatment did not influence the antioxidant status (α-tocopherol and total phenolic contents) and activities of LT (radical scavenging activity, ferric reducing antioxidant power and iron chelating activity). Feeding DCP significantly increased the proportion of conjugated linoleic acids and polyunsaturated fatty acids in beef. Lipid and colour stability of fresh beef patties stored in modified atmosphere packs (MAP) were unaffected by dietary treatment but feeding 40% DCP reduced (P < .05) lipid oxidation in aerobically-stored cooked beef patties. Beef patties stored in MAP for up to 7 days were assessed by sensory panellists to be juicier for those fed 40% DCP compared to 0% and 80% DCP. Results indicated that substitution of barley with DCP improved the fatty acid profiles of beef without negatively influencing the eating quality of beef.

Supplementation with different non-fiber carbohydrate sources in dairy cow diets with high or low rumen-undegradable protein content

ABSTRACT The objective for this study was to evaluate the supplementation with different non-fibrous carbohydrate (NFC) sources for grazing dairy cows in diets with high or low concentrations of rumen undegradable protein (RUP). Twelve multiparous cows averaging 30kg/d of milk production and 45±23 days in milk in a 4x4 Latin square design with 2 × 2 factorial arrangement were used. The difference in NFC content of the diets was achieved using ground corn (GC) or dried citrus pulp (DCP). Dietary RUP was altered by the partial replacement of soybean meal (LRUP) by roasted whole soybean and corn gluten meal (HRUP). Greater dry matter intake (DMI) was observed in DCP treatments compared to GC treatments (19.2 and 17.7kg/day of DM, respectively). Milk fat concentration was lower for LRUP treatments (3.4%) when compared to HRUP (3.6%). The milk protein concentration was greater for GC treatments (2.9%) in relation to DCP treatments (2.8%). Lower milk ureic nitrogen was observed when DCP diets were fed compared to those with GC (17.8 and 21.2mg/dL, respectively). The use of DCP as CNF source increased dry matter intake as well as milk fat concentration, although no differences in milk production were observed for RUP level.

Wet citrus pulp in finishing diets for feedlot lambs:

Pectin industry generates a citrus pulp residue compounded by peduncle, endocarp, juice vesicles, columella, seeds and tiny fractions of epicarp and mesocarp of citrus fruits, denominated Wet Citrus Pulp (WCP), which has not yet been tested as food for lamb’s nutrition. Thus, this study aimed to determine the effect of partial replacement of ground corn by WCP in high-concentrate diets on the performance and hepatic enzyme concentration of feedlot lambs. Forty-two male lambs (15 Santa Inês and 27 ½ Dorper × Santa Inês), with 24.7 ± 1.5 kg of BW and 60 ± 5 d of age was assigned to a randomized complete block design. Within blocks (n = 14), lambs were randomly assigned to 1 of 3 treatments: 0WCP (control) – diet containing 75.5% ground corn without WCP; 20WCP – diet containing 20% WCP in replacement of ground corn, and 40WCP – diet containing 40% WCP in replacement of ground corn. The experiment lasted 70 days, which was split in 3 experimental periods (14 days of diets’ adaptation and 2 sub-periods of 28 days each). Statistical analyses were performed using the MIXED procedure of the SAS. Orthogonal polynomials for diet response were determined by linear and quadratic effects. There was a quadratic effect for DM, CP, ash, ether extract and NFC intake The highest DM and CP intake was observed for lambs fed 20WCP, however, the control diet increased the ash, ether extract and NFC intake. The increased levels of WCP decreased the ADG and feed efficiency (FE) during the adaptation period, however, did not affect the ADG and FE on periods 1 and 2. Consequently, the increased levels of WCP inclusion decreased linearly the BW. There was no effect of WCP inclusion in diets on Gamma-Glutamyl Transferase (GGT) concentration. However, there was a linear increase for Aspartate Aminotransferase (AST) concentration during the adaptation period, but without difference in other periods. In conclusion, adding up to 40% of WCP in finishing diets for feedlot lambs decrease performance during adaptation period, compromising the final body weight, but without damages effects on liver enzymes.

Chemical, nutritive, fermentation profile and gas production of citrus pulp silages, alone or combined with maize silage

Quality attributes of citrus pulp silages that were ensiled alone and combined with maize silage were determined. Fresh samples of lemon, orange and tangerine pulps, maize plants and their combinations were fermented in glass jars for 90 days at about 20 - 25 °C. Treatments included i) 100% maize silage as control (MS); ii) 100% lemon pulp silage (LPS); iii) 100% orange pulp silage (OPS); iv) 100% tangerine pulp silage (TPS); v) 50% LPS and 50% maize silage (LPS + MS); vi) 50% OPS and 50% MS; and vii) 50% TPS and 50% maize silage (TPS + MS). The pH differed among treatment groups. The highest and lowest pH values were recorded for MS group and the OPS + MS group, respectively (3.84 vs. 3.51). The highest dry matter (DM), crude fibre (CF), neutral detergent fibre (NDF), acid detergent fibre (ADF) and hemicellulose (HEM) were observed for MS (P &lt;0.01). Citrus pulp silages alone had significantly greater total digestible nutrients (TDN), organic matter (OM) and non-fibre carbohydrate (NFC) values than MS and the combined silages (P &lt;0.01). Maize silage had higher lactic acid (LA) (101.2 g/kg DM) and acetic acid (AA) (49.3 g/kg DM) concentrations than the citrus groups (P &lt;0.05). In vitro gas production (TG), methane (CH4), metabolizable energy (ME) and organic matter digestibility (OMD) of the silages were similar (P &gt;0.05). Thus, citrus pulps can be ensiled in spite of their moisture content. However, to increased DM and nutrient content, the citrus pulps should be ensiled with 50% maize.&#x0D; Keywords: fermentation metabolites, methane production, organic matter digestibility